Biophysics reports最新文献_第4页

β-adrenergic regulation of Ca²⁺ signaling in heart cells. β肾上腺素能调节心脏细胞中的 Ca2+ 信号传导。

Biophysics reports Pub Date : 2024-10-31 DOI: 10.52601/bpr.2024.240906

Bo Yang, Shi-Qiang Wang, Hua-Qian Yang

引用次数: 0

Ca²⁺ homeostasis: a potential target for cancer therapies. Ca2+ 稳态：癌症疗法的潜在靶点。

Biophysics reports Pub Date : 2024-10-31 DOI: 10.52601/bpr.2024.230023

Min Su, Shanliang Zheng, Hao Liu, Tie-Shan Tang, Ying Hu

引用次数: 0

Oligomeric rearrangement may regulate channel activity. 低聚物重排可调节通道活性。

Biophysics reports Pub Date : 2024-10-31 DOI: 10.52601/bpr.2023.230018

Yue Ren, Xue Yang, Yuequan Shen

引用次数: 0

The rectification of heterotypic Cx46/Cx50 gap junction channels depends on intracellular magnesium. 异型 Cx46/Cx50 间隙连接通道的整流取决于细胞内的镁。

Biophysics reports Pub Date : 2024-10-31 DOI: 10.52601/bpr.2024.240015

Honghong Chen, Donglin Bai

{"title":"The rectification of heterotypic Cx46/Cx50 gap junction channels depends on intracellular magnesium.","authors":"Honghong Chen, Donglin Bai","doi":"10.52601/bpr.2024.240015","DOIUrl":"10.52601/bpr.2024.240015","url":null,"abstract":"Gap junction (GJ) intercellular communication is crucial in many physiological and pathological processes. A GJ channel is formed by head-to-head docking of two hexameric hemichannels from two neighboring cells. Heterotypic GJ channels formed by two different homomeric connexin hemichannels often display rectification properties in the current-voltage relationship while the underlying mechanisms are not fully clear. Here we studied heterotypic Cx46/Cx50 GJs at a single GJ channel level. Our data showed unitary Cx46/Cx50 GJ channel conductance (γj) rectification when 5 mmol/L Mg2+ was included in the patch pipette solution, while no γj rectification was observed when no Mg2+ was added. Including 5 mmol/L Mg2+ in pipette solution significantly decreased the γj of homotypic Cx46 GJ with little change in homotypic Cx50 γj. A missense point variant in Cx46 (E43F) reduced the Mg2+-dependent reduction in γj of Cx46 E43F GJ, indicating that E43 might be partially responsible for Mg2+-dependent decrease in γj of Cx46. A comprehensive understanding of Mg2+ modulation of GJ at the individual channel level is useful in understanding factors in modulating GJ-mediated intercellular communication in health and diseases.","PeriodicalId":93906,"journal":{"name":"Biophysics reports","volume":"10 5","pages":"336-348"},"PeriodicalIF":0.0,"publicationDate":"2024-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11554582/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142633962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Golgi Ca²⁺ stores, and original contributions by Prof. Shao Bai Xue. 高尔基体的 Ca2+ 储存，以及邵白雪教授的原创性贡献。

Biophysics reports Pub Date : 2024-10-31 DOI: 10.52601/bpr.2023.230015

Zong Jie Cui

引用次数: 0

Assaying sarcoplasmic reticulum Ca²⁺-leak in mouse atrial myocytes. 测定小鼠心房肌细胞肌质网 Ca2+ 泄漏。

Biophysics reports Pub Date : 2024-10-31 DOI: 10.52601/bpr.2023.230044

Fan Xu, Jing-Jing Li, Eric Yang, Yi Zhang, Wenjun Xie

{"title":"Assaying sarcoplasmic reticulum Ca2+-leak in mouse atrial myocytes.","authors":"Fan Xu, Jing-Jing Li, Eric Yang, Yi Zhang, Wenjun Xie","doi":"10.52601/bpr.2023.230044","DOIUrl":"10.52601/bpr.2023.230044","url":null,"abstract":"More and more studies have suggested an essential role of sarcoplasmic reticulum (SR) Ca2+ leak of atrial myocytes in atrial diseases such as atrial fibrillation (AF). The increasing interest in atrial Ca2+ signaling makes it necessary to develop a more accurate approach for Ca2+ measurement in atrial myocytes due to obvious differences between atrial and ventricular Ca2+ handling. In the present study, we proposed a new approach for quantifying total SR Ca2+ leak in atrial myocytes with confocal line-scan Ca2+ images. With a very precious approximation of the histogram of normalized line-scan Ca2+ images by using a modified Gaussian distribution, we separated the signal pixel components from noisy pixels and extracted two new dimensionless parameters, F signals and R signals, to reflect the summation of signal pixels and their release components, respectively. In the presence of tetracaine blocking SR Ca2+ leak, the two parameters were very close to 0, and in atrial myocytes under normal conditions, the two parameters are well positive correlative with Ca2+ spark frequency and total signal mass, the two classic readouts for SR Ca2+ leak. Consistent with Ca2+ Spark readouts, the two parameters quantified a significant increase of SR Ca2+ leak in atrial myocytes from mice harboring a leaky type 2 ryanodine receptor mutation (RyR2-R2474S+/-) compared to the WT group. Collectively, this study proposed a simple and effective approach to quantify SR Ca2+ leak in atrial myocytes, which may benefit research on calcium signaling in atrial physiology and diseases.","PeriodicalId":93906,"journal":{"name":"Biophysics reports","volume":"10 5","pages":"297-303"},"PeriodicalIF":0.0,"publicationDate":"2024-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11554581/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142633514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Modeling sarcoplasmic reticulum Ca²⁺ in rat cardiomyocytes. 建立大鼠心肌细胞肌浆网 Ca2+ 模型

Biophysics reports Pub Date : 2024-10-31 DOI: 10.52601/bpr.2024.240012

Yutong Su, Yongshen Liang, Menghao Xu, Beibei Gao, Siyuan Zhang, Eric Yang, Shuai Yin, Da Li, Zhangqin Huang, Wenjun Xie

{"title":"Modeling sarcoplasmic reticulum Ca2+ in rat cardiomyocytes.","authors":"Yutong Su, Yongshen Liang, Menghao Xu, Beibei Gao, Siyuan Zhang, Eric Yang, Shuai Yin, Da Li, Zhangqin Huang, Wenjun Xie","doi":"10.52601/bpr.2024.240012","DOIUrl":"10.52601/bpr.2024.240012","url":null,"abstract":"The sarcoplasmic reticulum (SR) primarily serves as the intracellular Ca2+ store in cardiac myocytes, mediating cellular function under cardiac physiology and diseases. However, the properties of cardiac SR Ca2+ have not yet been fully determined, particularly in rats and mice, which are the most commonly used experimental species in studies on cardiac physiology and diseases. Here, we developed a spatially detailed numerical model to deduce Ca2+ movements inside the junctional SR (jSR) cisternae of rat cardiomyocytes. Our model accurately reproduced the jSR Ca2+ kinetics of local and global SR Ca2+ releases reported in a recent experimental study. With this model, we revealed that jSR Ca2+ kinetics was mostly determined by the total release flux via type 2 ryanodine receptor (RyR2) channels but not by RyR2 positioning. Although Ca2+ diffusion in global SR was previously reported to be slow, our simulation demonstrated that Ca2+ diffused very quickly inside local jSR cisternae and the decrease in the diffusion coefficient resulted in a significant reduction of jSR Ca2+ depletion amplitude. Intracellular Ca2+ was typically experimentally detected with fluorescence dye. Our simulation revealed that when the dynamical characteristics of fluorescence dye exerted a minimal effect on actual Ca2+ mobility inside jSR, the reaction rate of the dye with Ca2+ could significantly affect apparent jSR Ca2+ kinetics. Therefore, loading a chemical fluorescence dye with fast kinetics, such as Fluo-5N, into SR is important for Ca2+ measurement inside SR. Overall, our model provides new insights into deciphering Ca2+ handling inside nanoscopic jSR cisternae in rat cardiomyocytes.","PeriodicalId":93906,"journal":{"name":"Biophysics reports","volume":"10 5","pages":"328-335"},"PeriodicalIF":0.0,"publicationDate":"2024-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11554579/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142633823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ca²⁺ sparks and beyond. Ca2+ 火花及其他

Biophysics reports Pub Date : 2024-10-31 DOI: 10.52601/bpr.2024.240909

Shi-Qiang Wang

引用次数: 0

Single-cell transcriptomics reveals neural stem cell trans-differentiation and cell subpopulations in whole heart decellularized extracellular matrix. 单细胞转录组学揭示了全心脱细胞细胞外基质中神经干细胞的跨分化和细胞亚群。

Biophysics reports Pub Date : 2024-08-31 DOI: 10.52601/bpr.2024.240011

Xiaoning Yang, Yuwei Zhao, Wei Liu, Zhongbao Gao, Chunlan Wang, Changyong Wang, Siwei Li, Xiao Zhang

{"title":"Single-cell transcriptomics reveals neural stem cell trans-differentiation and cell subpopulations in whole heart decellularized extracellular matrix.","authors":"Xiaoning Yang, Yuwei Zhao, Wei Liu, Zhongbao Gao, Chunlan Wang, Changyong Wang, Siwei Li, Xiao Zhang","doi":"10.52601/bpr.2024.240011","DOIUrl":"https://doi.org/10.52601/bpr.2024.240011","url":null,"abstract":"The whole heart decellularized extracellular matrix (ECM) has become a promising scaffold material for cardiac tissue engineering. Our previous research has shown that the whole heart acellular matrix possesses the memory function regulating neural stem cells (NSCs) trans-differentiating to cardiac lineage cells. However, the cell subpopulations and phenotypes in the trans-differentiation of NSCs have not been clearly identified. Here, we performed single-cell RNA sequencing and identified 2,765 cells in the recellularized heart with NSCs revealing the cellular diversity of cardiac and neural lineage, confirming NSCs were capable of trans-differentiating into the cardiac lineage while maintaining the original ability to differentiate into the neural lineage. Notably, the trans-differentiated heart-like cells have dual signatures of neuroectoderm and cardiac mesoderm. This study unveils an in-depth mechanism underlying the trans-differentiation of NSCs and provides a new opportunity and theoretical basis for cardiac regeneration.","PeriodicalId":93906,"journal":{"name":"Biophysics reports","volume":"10 4","pages":"241-253"},"PeriodicalIF":0.0,"publicationDate":"2024-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11399890/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142303327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Met1-linked ubiquitination in cell signaling regulation. 细胞信号调节中与 Met1 链接的泛素化。

Biophysics reports Pub Date : 2024-08-31 DOI: 10.52601/bpr.2024.230030

Yanmin Guo, Yuqin Zhao, Yu-Sheng Cong

引用次数: 0