Biophysics reports最新文献_第4页

Real-time analysis of nanoscale dynamics in membrane protein insertion via single-molecule imaging. 利用单分子成像技术实时分析膜蛋白插入的纳米级动态。

Biophysics reports Pub Date : 2024-12-31 DOI: 10.52601/bpr.2024.240024

Chenguang Yang, Dongfei Ma, Shuxin Hu, Ming Li, Ying Lu

引用次数: 0

A rapid and reproducible method for generating germ-free Drosophila melanogaster. 一种快速、可复制的产生无菌黑腹果蝇的方法。

Biophysics reports Pub Date : 2024-12-31 DOI: 10.52601/bpr.2024.240025

An-Qi Li, Sha-Sha Li, Hui-Lin Li, Lan-Lan Zhong, Guo-Bao Tian, Xin-Yuan Zhao, Qiao-Ping Wang

引用次数: 0

Thirty years of Ca²⁺ spark research: digital principle of cell signaling unveiled. 三十年 Ca2+ 火花研究：揭示细胞信号传递的数字原理。

Biophysics reports Pub Date : 2024-10-31 DOI: 10.52601/bpr.2024.240031

Fujian Lu, Pengcheng Yang, Donghui Zhang, Xianhua Wang, Heping Cheng

引用次数: 0

A bright cyan fluorescence calcium indicator for mitochondrial calcium with minimal interference from physiological pH fluctuations. 线粒体钙的亮青色荧光钙指示剂，受生理 pH 值波动的干扰极小。

Biophysics reports Pub Date : 2024-10-31 DOI: 10.52601/bpr.2024.240001

Wenjia Gu, Yuqin Yang, Yuqing Wang, Jia Li, Wanjie Li, Xiaoyan Zhang, Hao Dong, Youjun Wang

{"title":"A bright cyan fluorescence calcium indicator for mitochondrial calcium with minimal interference from physiological pH fluctuations.","authors":"Wenjia Gu, Yuqin Yang, Yuqing Wang, Jia Li, Wanjie Li, Xiaoyan Zhang, Hao Dong, Youjun Wang","doi":"10.52601/bpr.2024.240001","DOIUrl":"10.52601/bpr.2024.240001","url":null,"abstract":"Genetically Encoded Calcium (Ca2+) indicators (GECIs) are indispensable tools for dissecting intracellular Ca2+ signaling and monitoring cellular activities. Mitochondrion acts as a Ca2+ sink and a central player for maintaining Ca2+ homeostasis. Accurately monitoring Ca2+ transients within the mitochondrial matrix that undergo constant pH fluctuations is challenging, as signals of most currently available GECIs suffer from artifacts induced by physiological pH variations. Multiplexed monitoring of optophysiology is also hindered by the limited availability of GECIs with cyan fluorescence. Based on the bright variant of cyan fluorescence protein (CFP), mTurquoise2, we developed a GECI designated as TurCaMP. Results from molecular dynamics simulations and ab initio calculations revealed that the deprotonation of the chromophore may be responsible for the Ca2+-dependent changes in TurCaMP signals. TurCaMP sensors showed inverse response to Ca2+ transients, and their responses were not affected by pH changes within the range of pH 6-9. The high basal fluorescence and insensitivity to physiological pH fluctuations enabled TurCaMP to faithfully monitor mitochondrial Ca2+ responses with a high signal-to-noise ratio. TurCaMP sensors allow simultaneous multi-colored imaging of intracellular Ca2+ signals, expanding the possibility of multiplexed monitoring of Ca2+-dependent physiological events.","PeriodicalId":93906,"journal":{"name":"Biophysics reports","volume":"10 5","pages":"315-327"},"PeriodicalIF":0.0,"publicationDate":"2024-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11554577/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142635763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Simultaneous detection of dynamic calcium signaling and ERK activity in living cells. 同时检测活细胞中的动态钙信号和 ERK 活性。

Biophysics reports Pub Date : 2024-10-31 DOI: 10.52601/bpr.2023.230038

Liting Zhang, Yan Mo, Shimin Mo, Ming Xia, Chaoliang Wei

{"title":"Simultaneous detection of dynamic calcium signaling and ERK activity in living cells.","authors":"Liting Zhang, Yan Mo, Shimin Mo, Ming Xia, Chaoliang Wei","doi":"10.52601/bpr.2023.230038","DOIUrl":"10.52601/bpr.2023.230038","url":null,"abstract":"Calcium (Ca2+) is a universal second messenger in eukaryotic cells, and extracellular regulated protein kinases (ERK) are the core component of the mitogen-activated protein kinase (MAPK) signaling cascade. Both are involved in numerous physiological and pathological processes, such as organogenesis, tumorigenesis, proliferation, migration and apoptosis. Over the past decade, it has been found that calcium signaling can regulate the ERK activity through multiple mechanisms, and conversely, ERK signaling transduction can also affect the triggering and intensity of calcium signaling. However, there are few reports on how to perform real-time synchronous detection of these two signals. Here we described a method for dynamically and synchronously recording calcium signals and ERK activity in living cells, utilizing stable expression of multiple genetically-encoded probes and multi-channel synchronous detection technology using confocal microscopy. The protocol can be useful to address the spatiotemporal encoding dynamic mechanism of calcium signaling and ERK activity in single or multiple cells, and to reveal the interaction and causal characteristics of these two signals.","PeriodicalId":93906,"journal":{"name":"Biophysics reports","volume":"10 5","pages":"304-314"},"PeriodicalIF":0.0,"publicationDate":"2024-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11554583/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142633920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

β-adrenergic regulation of Ca²⁺ signaling in heart cells. β肾上腺素能调节心脏细胞中的 Ca2+ 信号传导。

Biophysics reports Pub Date : 2024-10-31 DOI: 10.52601/bpr.2024.240906

Bo Yang, Shi-Qiang Wang, Hua-Qian Yang

引用次数: 0

Ca²⁺ homeostasis: a potential target for cancer therapies. Ca2+ 稳态：癌症疗法的潜在靶点。

Biophysics reports Pub Date : 2024-10-31 DOI: 10.52601/bpr.2024.230023

Min Su, Shanliang Zheng, Hao Liu, Tie-Shan Tang, Ying Hu

引用次数: 0

Oligomeric rearrangement may regulate channel activity. 低聚物重排可调节通道活性。

Biophysics reports Pub Date : 2024-10-31 DOI: 10.52601/bpr.2023.230018

Yue Ren, Xue Yang, Yuequan Shen

引用次数: 0

The rectification of heterotypic Cx46/Cx50 gap junction channels depends on intracellular magnesium. 异型 Cx46/Cx50 间隙连接通道的整流取决于细胞内的镁。

Biophysics reports Pub Date : 2024-10-31 DOI: 10.52601/bpr.2024.240015

Honghong Chen, Donglin Bai

{"title":"The rectification of heterotypic Cx46/Cx50 gap junction channels depends on intracellular magnesium.","authors":"Honghong Chen, Donglin Bai","doi":"10.52601/bpr.2024.240015","DOIUrl":"10.52601/bpr.2024.240015","url":null,"abstract":"Gap junction (GJ) intercellular communication is crucial in many physiological and pathological processes. A GJ channel is formed by head-to-head docking of two hexameric hemichannels from two neighboring cells. Heterotypic GJ channels formed by two different homomeric connexin hemichannels often display rectification properties in the current-voltage relationship while the underlying mechanisms are not fully clear. Here we studied heterotypic Cx46/Cx50 GJs at a single GJ channel level. Our data showed unitary Cx46/Cx50 GJ channel conductance (γj) rectification when 5 mmol/L Mg2+ was included in the patch pipette solution, while no γj rectification was observed when no Mg2+ was added. Including 5 mmol/L Mg2+ in pipette solution significantly decreased the γj of homotypic Cx46 GJ with little change in homotypic Cx50 γj. A missense point variant in Cx46 (E43F) reduced the Mg2+-dependent reduction in γj of Cx46 E43F GJ, indicating that E43 might be partially responsible for Mg2+-dependent decrease in γj of Cx46. A comprehensive understanding of Mg2+ modulation of GJ at the individual channel level is useful in understanding factors in modulating GJ-mediated intercellular communication in health and diseases.","PeriodicalId":93906,"journal":{"name":"Biophysics reports","volume":"10 5","pages":"336-348"},"PeriodicalIF":0.0,"publicationDate":"2024-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11554582/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142633962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Golgi Ca²⁺ stores, and original contributions by Prof. Shao Bai Xue. 高尔基体的 Ca2+ 储存，以及邵白雪教授的原创性贡献。

Biophysics reports Pub Date : 2024-10-31 DOI: 10.52601/bpr.2023.230015

Zong Jie Cui

引用次数: 0