Biophysics reports最新文献

Intracellular calcium imaging for agonist screening. 细胞内钙显像用于激动剂筛选。

Biophysics reports Pub Date : 2025-06-30 DOI: 10.52601/bpr.2024.240026

Haojie Wang, Bo Yang, Liangzhu Mo, Hua-Qian Yang

引用次数: 0

Neuronal synaptic architecture revealed by cryo-correlative light and electron microscopy. 低温相关光镜和电镜显示的神经元突触结构。

Biophysics reports Pub Date : 2025-06-30 DOI: 10.52601/bpr.2024.240035

Pei Wang, Buyun Tian, Xiaojun Xu, Huiqin Luan, Yan Zhang, Wenhao Sun, Liqiao Hu, Yuanyuan Li, Yuchen Yao, Weixing Li, Shuli Zhang, Xia Li, Wei Feng, Wei Ji, Yanhong Xue

引用次数: 0

Modulating mitochondrial dynamics in CMT2A: a multifaceted platform for drug discovery and evaluation. 调节线粒体动力学在CMT2A：药物发现和评估的多方面平台。

Biophysics reports Pub Date : 2025-06-30 DOI: 10.52601/bpr.2024.240037

Yang Liu, Chen Yan, Borui Cao, Dejun Kong, Jiaqi Li, Wenlei Li, Yingjie Guo, Zhongyang Yuan, Yumiao Gao, Yubo Zhang, Ran Sui, Guo Chen, Xiaojiang Hao, Quan Chen

{"title":"Modulating mitochondrial dynamics in CMT2A: a multifaceted platform for drug discovery and evaluation.","authors":"Yang Liu, Chen Yan, Borui Cao, Dejun Kong, Jiaqi Li, Wenlei Li, Yingjie Guo, Zhongyang Yuan, Yumiao Gao, Yubo Zhang, Ran Sui, Guo Chen, Xiaojiang Hao, Quan Chen","doi":"10.52601/bpr.2024.240037","DOIUrl":"10.52601/bpr.2024.240037","url":null,"abstract":"Mitochondrial dynamics, encompassing fusion and fission processes, plays a crucial role in regulating mitochondrial distribution, motility, and material exchange within cells, particularly in the nervous system. Mitofusin-2 (MFN2), a GTPase localized to the outer mitochondrial membrane, mediates mitochondrial fusion through dimerization and conformational changes. Mutations in MFN2 are causal for Charcot-Marie-Tooth disease type 2A (CMT2A), an inherited peripheral neuropathy for which no curative treatment currently exists. Herein, we have developed a comprehensive mitochondrial drug-screening and evaluation platform to facilitate the identification of potential therapeutic candidates. This work builds upon our previous research with S89, a small molecule agonist derived from spiramine alkaloids that promotes mitochondrial fusion by interacting with endogenous MFN1 and effectively mitigates axonal degeneration in CMT2A patient-derived motor neurons. This platform integrates three sequential stages of assessment: (1) initial screening in Mfn knockout mouse embryonic fibroblasts (MEFs) to identify compounds capable of reversibly rescuing mitochondrial fragmentation; (2) evaluation in primary neuronal cultures derived from CMT2A mouse dorsal root ganglia and cortex to assess the compounds' efficacy in restoring mitochondrial morphology, axonal transport, and neurite outgrowth; and (3) final assessment in CMT2A patient-derived induced pluripotent stem cell (iPSC)-differentiated motor neurons to determine the candidates' therapeutic potential in human peripheral nervous system cells. This multi-tiered approach facilitates rapid compound screening with increasing physiological relevance, enhancing the efficiency and translational potential of identifying therapeutic candidates for CMT2A.","PeriodicalId":93906,"journal":{"name":"Biophysics reports","volume":"11 3","pages":"143-155"},"PeriodicalIF":0.0,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12213711/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144562346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An integrative approach for imaging and quantitative analysis of gut microbiota growth in vivo using fluorescent D-amino acid labeling and fluorescence in situ hybridization. 利用荧光d -氨基酸标记和荧光原位杂交技术对体内肠道微生物群生长进行成像和定量分析的综合方法。

Biophysics reports Pub Date : 2025-06-30 DOI: 10.52601/bpr.2024.240044

Yingjun Zhou, Liyuan Lin, Wei Wang

{"title":"An integrative approach for imaging and quantitative analysis of gut microbiota growth in vivo using fluorescent D-amino acid labeling and fluorescence in situ hybridization.","authors":"Yingjun Zhou, Liyuan Lin, Wei Wang","doi":"10.52601/bpr.2024.240044","DOIUrl":"10.52601/bpr.2024.240044","url":null,"abstract":"The profound influence of gut microbiota on human health has been well-recognized; however, substantial gaps remain in our understanding of the highly diverse and dynamic processes of microbial growth and activities in the gut. Conventional methods, which primarily rely on DNA sequencing, provide limited insights into these aspects. This paper presents a protocol that integrates fluorescent D-amino acid (FDAA) metabolic labeling with fluorescence in situ hybridization (FISH) for imaging and quantitatively analyzing the in vivo growth of gut microbiota. By administering two FDAAs sequentially through mouse gavage, we label the peptidoglycan of gut bacteria in their native environment, allowing the labeling signals on bacterial cell walls to serve as markers of cellular proliferation and division. We have also demonstrated that the intensity of FDAA labeling directly correlates with the metabolic activity of gut bacteria. Additionally, FISH is employed to distinguish specific bacterial taxa of interest via fluorescence microscopy or flow cytometry. This integrative method greatly enhances our capacity to visualize and measure the in vivo growth and metabolic states of various gut bacteria, thereby illuminating the previously obscured \"dark matter\" in the gut ecosystem.","PeriodicalId":93906,"journal":{"name":"Biophysics reports","volume":"11 3","pages":"172-179"},"PeriodicalIF":0.0,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12213662/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144562341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synovial sarcoma extracellular vesicles induce fatty liver. 滑膜肉瘤细胞外囊泡诱发脂肪肝。

Biophysics reports Pub Date : 2025-06-30 DOI: 10.52601/bpr.2024.240041

Tao Ren, Zhiheng Zhou, Huirong Hong, Bohong Cen, Jun Xiao, Guofen Chen, Yang Zhang, Jianlong Li

{"title":"Synovial sarcoma extracellular vesicles induce fatty liver.","authors":"Tao Ren, Zhiheng Zhou, Huirong Hong, Bohong Cen, Jun Xiao, Guofen Chen, Yang Zhang, Jianlong Li","doi":"10.52601/bpr.2024.240041","DOIUrl":"10.52601/bpr.2024.240041","url":null,"abstract":"Synovial sarcoma leads to pathological changes in multiple organs. To investigate the mechanism by which synovial sarcoma induces fatty liver through extracellular vesicles (EVs), the synovial sarcoma SW-982 cells were orthotopically implanted, or SW-982 derived EVs were extracted and used to \"educate\" nude mice. Liver tissues were then subjected to H&E and Oil-Red O (ORO) staining, and qPCR analysis. EVs were characterized using TEM and Nanosight. The bio-distribution of EVs in vivo was verified using the fluorescent dye Burgundy staining, followed by Odyssey imaging. Immunofluorescence (IF) and flow cytometry were used to confirm cellular uptake of EVs. Rab27a knockdown (KD) efficiency was validated by Western blot, and lipid droplet deposition in the liver from mice bearing with SW-982-Rab27a-KD cells was observed by staining with ORO. After three weeks of orthotopic implantation of SW-982 cells in nude mice, qPCR and H&E showed no tumor metastasis in liver tissues, while ORO staining revealed lipid deposition in the liver, and EVs diameters were confirmed by Nanosight and TEM to be approximately 141 nm in size. In vivo, EVs were taken up by liver Kupffer cells (KCs). After \"educating\" nude mice with EVs, lipid deposition in the liver was observed. In rescue experiments, Rab27a knockdown reduced EV secretion from the tumor, and KC inactivation led to decreased lipid deposition in the liver. It is shown that synovial sarcoma EVs mediate fatty liver through Kupffer cells.","PeriodicalId":93906,"journal":{"name":"Biophysics reports","volume":"11 3","pages":"209-216"},"PeriodicalIF":0.0,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12213661/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144562265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring diet-microbiota interactions and therapeutic nutrition management in inflammatory bowel disease. 探讨炎症性肠病的饮食-微生物群相互作用和治疗性营养管理。

Biophysics reports Pub Date : 2025-06-30 DOI: 10.52601/bpr.2024.240050

Xinran Wang, Yiran Wang, Lulu Sun

{"title":"Exploring diet-microbiota interactions and therapeutic nutrition management in inflammatory bowel disease.","authors":"Xinran Wang, Yiran Wang, Lulu Sun","doi":"10.52601/bpr.2024.240050","DOIUrl":"10.52601/bpr.2024.240050","url":null,"abstract":"Intestinal bowel disease (IBD) is a chronic, early-onset, recurrent gastrointestinal immune-related disease that has become globalized. Although the combination of genetic, environmental, and immunological factors leads to intestinal inflammation and barrier damage, the etiology of IBD is not clearly defined. In recent years, diet-microbiota interactions have been widely studied for their potential in pathogenesis and treatment for IBD. Meanwhile, the significant efficacy of exclusive enteral nutrition (EEN) has been observed in clinical practice with modulation in gut microbiota, but the specific mechanisms and optimization measures remain challenging. Therefore, we first describe the development of existing microbial research techniques and the perspectives that can be broadened. We then synthesize findings on how dietary components impact IBD progression and treatment through microbiota. Finally, based on correlating clinical and basic experiments, we summarize the current status and potential mechanisms of EEN for treating IBD, especially the contradictory points encountered in its application.","PeriodicalId":93906,"journal":{"name":"Biophysics reports","volume":"11 3","pages":"180-197"},"PeriodicalIF":0.0,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12213664/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144562344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assaying antigen-specific T cell trans-endothelial migration in vitro with the Transwell system: application in tumor immunology. 用Transwell系统测定抗原特异性T细胞体外跨内皮迁移：在肿瘤免疫学中的应用。

Biophysics reports Pub Date : 2025-06-30 DOI: 10.52601/bpr.2024.240032

Ruochan Zhang, Longchao Liu

引用次数: 0

Correction to: Non-invasive micro-test technology and applications. 非侵入性微检测技术及应用。

Biophysics reports Pub Date : 2025-06-30 DOI: 10.52601/bpr.2025.250901

引用次数: 0

Electrophysiological analysis of cardiac K_ATP channel. 心脏KATP通道电生理分析。

Biophysics reports Pub Date : 2025-04-30 DOI: 10.52601/bpr.2024.240023

Jianyi Huo, Hua-Qian Yang

引用次数: 0

Proteomic analysis of B cells in peripheral lymphatic system reveals the dynamics during the systemic lupus erythematosus progression. 外周淋巴系统B细胞的蛋白质组学分析揭示了系统性红斑狼疮进展过程中的动力学。

Biophysics reports Pub Date : 2025-04-30 DOI: 10.52601/bpr.2024.240045

Liming Sun, Yuanyuan Yin, Yuqing Cao, Chunlei Chen, Yutong Guo, Zeming Cai, Jiarui Wu, Qingrun Li

{"title":"Proteomic analysis of B cells in peripheral lymphatic system reveals the dynamics during the systemic lupus erythematosus progression.","authors":"Liming Sun, Yuanyuan Yin, Yuqing Cao, Chunlei Chen, Yutong Guo, Zeming Cai, Jiarui Wu, Qingrun Li","doi":"10.52601/bpr.2024.240045","DOIUrl":"https://doi.org/10.52601/bpr.2024.240045","url":null,"abstract":"In this study, we conducted a comprehensive proteomic analysis of B cells from the spleen, mesenteric lymph nodes (mLN), and peripheral blood mononuclear cells (PBMC) in a time-course model of systemic lupus erythematosus (SLE) using female MRL/lpr mice. By combining fluorescence-activated cell sorting (FACS) and 4D-Data-Independent Acquisition (4D-DIA) mass spectrometry, we quantified nearly 8000 proteins, identifying significant temporal and tissue-specific proteomic changes during SLE progression. PBMC-derived B cells exhibited early proteomic alterations by Week 9, while spleen-derived B cells showed similar changes by Week 12. We identified key regulatory proteins, including BAFF, BAFFR, and NFKB2, involved in B cell survival and activation, as well as novel markers such as CD11c and CD117, which have previously been associated with other immune cells. The study highlights the dynamic reprogramming of B cell proteomes across different tissues, with distinct contributions to SLE pathogenesis, providing valuable insights into the molecular mechanisms underlying B cell dysregulation in lupus. These findings offer potential therapeutic targets and biomarkers for SLE.","PeriodicalId":93906,"journal":{"name":"Biophysics reports","volume":"11 2","pages":"129-142"},"PeriodicalIF":0.0,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12035744/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144051958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0