Biophysics reports最新文献_第3页

Characterization of auditory sensation in C. elegans. 秀丽隐杆线虫听觉感觉的表征。

Biophysics reports Pub Date : 2024-12-31 DOI: 10.52601/bpr.2024.240027

Can Wang, Elizabeth A Ronan, Adam J Iliff, Rawan Al-Ebidi, Panagiota Kitsopoulos, Karl Grosh, Jianfeng Liu, X Z Shawn Xu

{"title":"Characterization of auditory sensation in C. elegans.","authors":"Can Wang, Elizabeth A Ronan, Adam J Iliff, Rawan Al-Ebidi, Panagiota Kitsopoulos, Karl Grosh, Jianfeng Liu, X Z Shawn Xu","doi":"10.52601/bpr.2024.240027","DOIUrl":"10.52601/bpr.2024.240027","url":null,"abstract":"Research using the model organism nematode C. elegans has greatly facilitated our understanding of sensory biology, including touch, olfaction, taste, vision and proprioception. While hearing had long been considered to be restricted to vertebrates and some arthropods, we recently discovered that C. elegans is capable of sensing and responding to airborne sound in a frequency and sound source-size-dependent manner. C. elegans auditory sensation occurs when airborne sound physically vibrates their external cuticle (skin) to activate the sound-sensitive mechanosensory FLP/PVD neurons via nicotinic acetylcholine receptors (nAChRs), triggering aversive phonotaxis behavior. Here, we report stepwise methods to characterize these three features of C. elegans auditory sensation, including sound-evoked skin vibration, neuronal activation, and behavior. This approach provides an accessible platform to investigate the cellular and molecular mechanisms underlying auditory sensation and mechanotransduction mechanisms in C. elegans.","PeriodicalId":93906,"journal":{"name":"Biophysics reports","volume":"10 6","pages":"351-363"},"PeriodicalIF":0.0,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11693501/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142933888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mechanism and application of mesenchymal stem cells and their secreting extracellular vesicles in regulating CD4⁺T cells in immune diseases. 间充质干细胞及其分泌胞外囊泡在免疫疾病中调节CD4+T细胞的机制及应用

Biophysics reports Pub Date : 2024-12-31 DOI: 10.52601/bpr.2024.240005

Zehua Lin, Weisong Cai, Yuechen Sun, Baoai Han, Yifan Hu, Zuhong He, Xiong Chen

{"title":"Mechanism and application of mesenchymal stem cells and their secreting extracellular vesicles in regulating CD4+T cells in immune diseases.","authors":"Zehua Lin, Weisong Cai, Yuechen Sun, Baoai Han, Yifan Hu, Zuhong He, Xiong Chen","doi":"10.52601/bpr.2024.240005","DOIUrl":"https://doi.org/10.52601/bpr.2024.240005","url":null,"abstract":"Mesenchymal stem cells (MSCs) show significant promise in treating immune diseases due to their ability to differentiate into various cell types and their immunomodulatory properties. However, the mechanisms by which MSCs regulate CD4+T cells, essential for immune responses, are not yet fully understood. This study aims to provide a comprehensive overview of how MSCs and their secreted extracellular vesicles (EVs) modulate CD4+T cells in immune diseases. We begin by discussing the immunomodulatory properties of MSCs and the factors contributing to their effectiveness. Following this, we explore how MSCs interact with CD4+T cells through various pathways, including the secretion of soluble factors, direct cell-cell contact, and EV-mediated communication. A key focus is on the therapeutic potential of MSC-derived EVs, which are rich in bioactive molecules such as proteins, lipids, and nucleic acids. These molecules can regulate the phenotype and function of CD4+T cells. The challenges and future perspectives in utilizing MSCs and EVs for immune-disease therapy are also addressed. Overall, this research aims to enhance our understanding of the mechanisms behind MSC-mediated regulation of CD4+T cells and provide insights into the potential use of MSCs and EVs as therapeutic tools in immune diseases. In summary, understanding how MSCs and their EVs control CD4+T cells can offer valuable perspectives for developing innovative immunotherapeutic approaches. Leveraging the immunomodulatory capacity of MSCs and EVs holds promise for managing immune-related disorders.","PeriodicalId":93906,"journal":{"name":"Biophysics reports","volume":"10 6","pages":"403-415"},"PeriodicalIF":0.0,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11693500/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142933943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Special issue on "calcium signaling". “钙信号”特刊。

Biophysics reports Pub Date : 2024-12-31 DOI: 10.52601/bpr.2024.240061

Zong Jie Cui

引用次数: 0

Current status of FAP-directed cancer theranostics: a bibliometric analysis. fap导向癌症治疗的现状：文献计量学分析。

Biophysics reports Pub Date : 2024-12-31 DOI: 10.52601/bpr.2024.240022

Dan Ruan, Simin Wu, Xuehua Lin, Liang Zhao, Jiayu Cai, Weizhi Xu, Yizhen Pang, Qiang Xie, Xiaobo Qu, Haojun Chen

{"title":"Current status of FAP-directed cancer theranostics: a bibliometric analysis.","authors":"Dan Ruan, Simin Wu, Xuehua Lin, Liang Zhao, Jiayu Cai, Weizhi Xu, Yizhen Pang, Qiang Xie, Xiaobo Qu, Haojun Chen","doi":"10.52601/bpr.2024.240022","DOIUrl":"https://doi.org/10.52601/bpr.2024.240022","url":null,"abstract":"Fibroblast activation protein (FAP) is a key molecule in the field of oncology, with significant impacts on tumor diagnosis and treatment. Importantly, it has paved the way for the development of radiotracers for quinoline-based FAP inhibitors (FAPIs), which are currently among the most promising radiotracers for PET imaging in cancer. We performed a bibliometric analysis of scientific publications related to FAP and FAPI-based radiotracers, which included the quantification and visualization of current research trends and prospects based on various bibliometric indicators. In our survey of FAP-related studies in the Web of Science Core Collection databases, R and VOSviewer were used for visualization and bibliometric analyses based on country, institute, author, journal, and keywords. We also examined the methodology, radionuclide type, imaging instruments, and major diseases associated with studies on FAPI-based radiotracers. The results revealed 2,664 FAP-related publications from 1992 to the present. Germany, the USA, and China dominated paper publications, multinational collaborations, and societal impacts on FAP research. Southwest Medical University was the most productive institute, while Haberkorn Uwe authored the most cited papers and the highest H-index. The European Journal of Nuclear Medicine and Molecular Imaging and the Journal of Nuclear Medicine were the most influential periodicals. Keywords \"FAP\", \"68Ga-FAPI\", and \"PET/CT\" emerged as the most significant in this field. This study may help elucidate current research trends, hotspots, and directions for future research.","PeriodicalId":93906,"journal":{"name":"Biophysics reports","volume":"10 6","pages":"388-402"},"PeriodicalIF":0.0,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11693499/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142933941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A prognostic model for acute myeloid leukemia based on ferroptosis-related lncRNA and immune infiltration analysis. 基于凋亡相关lncRNA和免疫浸润分析的急性髓系白血病预后模型

Biophysics reports Pub Date : 2024-12-31 DOI: 10.52601/bpr.2024.240029

Shuhan Liu, Yingli Chen, Qianzhong Li, Zhiyu Fan, Menglan Li, Pengyu Du

{"title":"A prognostic model for acute myeloid leukemia based on ferroptosis-related lncRNA and immune infiltration analysis.","authors":"Shuhan Liu, Yingli Chen, Qianzhong Li, Zhiyu Fan, Menglan Li, Pengyu Du","doi":"10.52601/bpr.2024.240029","DOIUrl":"https://doi.org/10.52601/bpr.2024.240029","url":null,"abstract":"Acute myeloid leukemia (AML) is a rare tumor that invades the blood and bone marrow, it is rapidly progressive, highly aggressive, and difficult to cure. Studies have shown that long non-coding RNA (lncRNA) and ferroptosis play important roles in AML. However, few studies have been done on ferroptosis-related lncRNA for AML. To investigate the role of ferroptosis-related lncRNA in AML prognosis, we screened the differentially expressed genes related to ferroptosis and lncRNA. Ferroptosis-related lncRNA associated with AML prognosis was obtained by Pearson correlation analysis. By using univariate Cox analysis, least absolute shrinkage and selection operator (LASSO) analysis, and multivariate Cox analysis, the ten prognostic genes were used for constructing the prognostic model. The model was then validated using a Kaplan-Meier analysis and Cox regression analysis. The ROC results have shown that the model could better predict AML survival. We identified some mutated genes that may affect the poor prognosis based on the somatic mutation analysis. The enrichment pathway analysis of prognostic genes revealed that these genes were mainly enriched in some immune pathways and cancer pathways. By immune infiltration analysis, we found that high-risk patients may respond better to immunotherapy.","PeriodicalId":93906,"journal":{"name":"Biophysics reports","volume":"10 6","pages":"377-387"},"PeriodicalIF":0.0,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11693502/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142933832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Real-time analysis of nanoscale dynamics in membrane protein insertion via single-molecule imaging. 利用单分子成像技术实时分析膜蛋白插入的纳米级动态。

Biophysics reports Pub Date : 2024-12-31 DOI: 10.52601/bpr.2024.240024

Chenguang Yang, Dongfei Ma, Shuxin Hu, Ming Li, Ying Lu

引用次数: 0

A rapid and reproducible method for generating germ-free Drosophila melanogaster. 一种快速、可复制的产生无菌黑腹果蝇的方法。

Biophysics reports Pub Date : 2024-12-31 DOI: 10.52601/bpr.2024.240025

An-Qi Li, Sha-Sha Li, Hui-Lin Li, Lan-Lan Zhong, Guo-Bao Tian, Xin-Yuan Zhao, Qiao-Ping Wang

引用次数: 0

Thirty years of Ca²⁺ spark research: digital principle of cell signaling unveiled. 三十年 Ca2+ 火花研究：揭示细胞信号传递的数字原理。

Biophysics reports Pub Date : 2024-10-31 DOI: 10.52601/bpr.2024.240031

Fujian Lu, Pengcheng Yang, Donghui Zhang, Xianhua Wang, Heping Cheng

引用次数: 0

A bright cyan fluorescence calcium indicator for mitochondrial calcium with minimal interference from physiological pH fluctuations. 线粒体钙的亮青色荧光钙指示剂，受生理 pH 值波动的干扰极小。

Biophysics reports Pub Date : 2024-10-31 DOI: 10.52601/bpr.2024.240001

Wenjia Gu, Yuqin Yang, Yuqing Wang, Jia Li, Wanjie Li, Xiaoyan Zhang, Hao Dong, Youjun Wang

{"title":"A bright cyan fluorescence calcium indicator for mitochondrial calcium with minimal interference from physiological pH fluctuations.","authors":"Wenjia Gu, Yuqin Yang, Yuqing Wang, Jia Li, Wanjie Li, Xiaoyan Zhang, Hao Dong, Youjun Wang","doi":"10.52601/bpr.2024.240001","DOIUrl":"10.52601/bpr.2024.240001","url":null,"abstract":"Genetically Encoded Calcium (Ca2+) indicators (GECIs) are indispensable tools for dissecting intracellular Ca2+ signaling and monitoring cellular activities. Mitochondrion acts as a Ca2+ sink and a central player for maintaining Ca2+ homeostasis. Accurately monitoring Ca2+ transients within the mitochondrial matrix that undergo constant pH fluctuations is challenging, as signals of most currently available GECIs suffer from artifacts induced by physiological pH variations. Multiplexed monitoring of optophysiology is also hindered by the limited availability of GECIs with cyan fluorescence. Based on the bright variant of cyan fluorescence protein (CFP), mTurquoise2, we developed a GECI designated as TurCaMP. Results from molecular dynamics simulations and ab initio calculations revealed that the deprotonation of the chromophore may be responsible for the Ca2+-dependent changes in TurCaMP signals. TurCaMP sensors showed inverse response to Ca2+ transients, and their responses were not affected by pH changes within the range of pH 6-9. The high basal fluorescence and insensitivity to physiological pH fluctuations enabled TurCaMP to faithfully monitor mitochondrial Ca2+ responses with a high signal-to-noise ratio. TurCaMP sensors allow simultaneous multi-colored imaging of intracellular Ca2+ signals, expanding the possibility of multiplexed monitoring of Ca2+-dependent physiological events.","PeriodicalId":93906,"journal":{"name":"Biophysics reports","volume":"10 5","pages":"315-327"},"PeriodicalIF":0.0,"publicationDate":"2024-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11554577/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142635763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Simultaneous detection of dynamic calcium signaling and ERK activity in living cells. 同时检测活细胞中的动态钙信号和 ERK 活性。

Biophysics reports Pub Date : 2024-10-31 DOI: 10.52601/bpr.2023.230038

Liting Zhang, Yan Mo, Shimin Mo, Ming Xia, Chaoliang Wei

{"title":"Simultaneous detection of dynamic calcium signaling and ERK activity in living cells.","authors":"Liting Zhang, Yan Mo, Shimin Mo, Ming Xia, Chaoliang Wei","doi":"10.52601/bpr.2023.230038","DOIUrl":"10.52601/bpr.2023.230038","url":null,"abstract":"Calcium (Ca2+) is a universal second messenger in eukaryotic cells, and extracellular regulated protein kinases (ERK) are the core component of the mitogen-activated protein kinase (MAPK) signaling cascade. Both are involved in numerous physiological and pathological processes, such as organogenesis, tumorigenesis, proliferation, migration and apoptosis. Over the past decade, it has been found that calcium signaling can regulate the ERK activity through multiple mechanisms, and conversely, ERK signaling transduction can also affect the triggering and intensity of calcium signaling. However, there are few reports on how to perform real-time synchronous detection of these two signals. Here we described a method for dynamically and synchronously recording calcium signals and ERK activity in living cells, utilizing stable expression of multiple genetically-encoded probes and multi-channel synchronous detection technology using confocal microscopy. The protocol can be useful to address the spatiotemporal encoding dynamic mechanism of calcium signaling and ERK activity in single or multiple cells, and to reveal the interaction and causal characteristics of these two signals.","PeriodicalId":93906,"journal":{"name":"Biophysics reports","volume":"10 5","pages":"304-314"},"PeriodicalIF":0.0,"publicationDate":"2024-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11554583/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142633920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0