Animal models and experimental medicine最新文献

{"title":"Animal models for abdominal aortic aneurysms: Where we are and where we need to go","authors":"Kangli Tian,&nbsp;Fizza Malik,&nbsp;Sihai Zhao","doi":"10.1002/ame2.12572","DOIUrl":"10.1002/ame2.12572","url":null,"abstract":"<p>The mortality rate of patients with abdominal aortic aneurysm (AAA) after rupture is extremely high, and this disease has become an important disease endangering the health of the Chinese population. Methods used to model AAA include intraluminal pressurized elastase infusion, chronic infusion of angiotensin II (Ang II) via an osmotic pump, periarterial application of calcium chloride, vascular grafting, and gene modification. AAA models induced by elastase and Ang II are the two most widely used animal models. In the elastase-induced model, because intraluminal infusion is transient, with the cessation of initial stimulation, the aneurysm lesion tends to be stable and rarely ruptures. The model induced by Ang II infusion often presents with a typical aortic dissection with a false lumen, whereas clinical AAA patients do not necessarily have dissection. Currently, the treatment of AAA in clinical practice remains endovascular, and there is a lack of pharmacological therapy, which is also related to the fact that the pathogenic mechanism has not been fully elucidated. Smoking, old age, male sex, and hypertension are the main risk factors for AAA, but these risk factors have not been fully investigated in the current modeling methods, which may affect the clinical translational application of research results based on animal models. Therefore, this article reviews the most commonly used AAA modeling methods, comments on their applications and limitations, and provides a perspective on the development of novel animal models.</p>","PeriodicalId":93869,"journal":{"name":"Animal models and experimental medicine","volume":"8 3","pages":"573-577"},"PeriodicalIF":0.0,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ame2.12572","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143191610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Building an international platform for the research, development, and dissemination of new knowledge in the field of laboratory animal science, technology, and experimental medicine","authors":"Muhammad Iqbal Choudhary","doi":"10.1002/ame2.12570","DOIUrl":"https://doi.org/10.1002/ame2.12570","url":null,"abstract":"<p>Dear Colleagues,</p><p>It is my distinct honor to serve as the Honorary Chief Editor of <i>AMEM</i>, an international open-access journal, dedicated to serve as a platform for international exchange, and the translation of research outcomes in the field of life sciences. <i>AMEM</i> strives to establish itself as a high-level international academic exchange platform that brings together scientific wisdom from around the world and offers a prestigious journal for presenting research findings, and disseminating state of the art knowledge in the vibrant fields of life sciences.</p><p><i>AMEM</i>'s strong academic foundation and extensive international influence are evident from its inclusion in esteemed databases, such as ESCI, MEDLINE, Scopus, PMC, and DOAJ, with a Q2 ranking in JCI and JCR, and widespread indexing by academic search engines including Google Scholar. The articles published in <i>AMEM</i> cover various frontier areas of animal model preparation, laboratory animal science, and experimental medicine, featuring special columns on medical artificial intelligence, stem cells and regenerative medicine, neurodegenerative diseases, and cardiovascular and cerebrovascular disease research, making it one of the key platform for dissemination of new and novel research, and developments to global readers. I am sure the untiring and sincere efforts of the Editorial team and contributing authors will help the <i>AMEM</i> to leap forward to greater heights.</p><p>Here, I sincerely invite researchers in various fields of life sciences to contribute original articles to <i>AMEM</i>. Whether you specialize in the development and utilization of experimental animal resources or are focused on experimental medical research on cancers, neurodegenerative diseases, and more, <i>AMEM</i> is an ideal choice for showcasing your research findings and promoting academic excellence. We greatly appreciate that your scholarly contributions will play a pivotal role in advancing experimental animal, and experimental medicine fields.</p><p>I sincerely hope that researchers worldwide will join hands with the <i>AMEM</i> team in our shared mission of showcasing the latest research findings and academic excellence from the platform of <i>AMEM</i>. Our team will make sure that your research breakthroughs reach every corner of the world.</p><p>Finally, I wish all of you continued success, and look forward to witnessing the sustained growth in the stature and outreach of the <i>AMEM</i>.</p><p>Thank you all!</p><p><i>AMEM</i> Honrary Editor-in-Chief </p>","PeriodicalId":93869,"journal":{"name":"Animal models and experimental medicine","volume":"8 1","pages":"4"},"PeriodicalIF":0.0,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ame2.12570","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143248810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of an atherosclerosis rabbit model to evaluate the hemodynamic impact of extracorporeal circulation","authors":"Anna Kathrin Assmann,&nbsp;Jan Buschmann,&nbsp;Sinje Reimers,&nbsp;Aleyna Karakas,&nbsp;Elvira Weber,&nbsp;Hug Aubin,&nbsp;Artur Lichtenberg,&nbsp;Alexander Assmann","doi":"10.1002/ame2.12556","DOIUrl":"10.1002/ame2.12556","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Aortic atherosclerosis increases the risk of embolic events under extracorporeal circulation (ECC). To evaluate the hemodynamic impact of ECC on atheromatous plaques, an atherosclerosis animal model, which is also eligible for ECC, is required.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Twenty-nine New Zealand White rabbits received a pro-atherosclerotic diet (group diet, <i>n</i> = 10), a pro-atherosclerotic diet and additional intraaortic balloon insufflation injury (group BI, <i>n</i> = 9), or served as controls (<i>n</i> = 10). After 3 or 6 months, aortic explants were analyzed by (immuno-)histology and RT-PCR.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Blood serum analyses revealed increased cholesterol-levels in groups diet and BI compared to controls (3 months: <i>p</i> = 0.03 each, 6 months: <i>p</i> &lt; 0.0001 each). Aortic inflammatory infiltration was significantly enhanced in groups diet (CD3 at 3 months: <i>p</i> &lt; 0.0001, 6 months: <i>p</i> = 0.02; CD68 at 3 months: <i>p</i> = 0.01) and BI (CD3 at 3 months: <i>p</i> &lt; 0.0001, 6 months: <i>p</i> = 0.03; CD68 at 3 months: <i>p</i> = 0.04, 6 months: <i>p</i> = 0.02). Increased intima hyperplasia occurred in both groups (<i>p</i> &lt; 0.0001 each). Macroscopic analyses after 3 and 6 months showed ubiquitous lumen-narrowing aortic plaques. Calcification of the intima and media was increased in groups diet (intima: <i>p</i> &lt; 0.0001 at 3 and 6 months; media at 3 months: <i>p</i> &lt; 0.0001, 6 months: <i>p</i> = 0.01) and BI (intima: <i>p</i> &lt; 0.0001 at 3 and 6 months; media at 3 months: <i>p</i> &lt; 0.0001, 6 months: <i>p</i> = 0.02). Extensive lipid accumulation was found in the intima in both treatment groups (<i>p</i> &lt; 0.0001 each).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>A rabbit model with high aortic calcific plaque burden—diet-induced with no implicit need of an additional intimal injury by an intraaortic balloon insufflation due to comparable outcome—exhibiting multiple pathophysiological aspects of human atherosclerosis has been designed and thoroughly characterized. It is suitable for use in future studies on the interaction between atherosclerotic plaques and the arterial blood flow under ECC.</p>\u0000 </section>\u0000 </div>","PeriodicalId":93869,"journal":{"name":"Animal models and experimental medicine","volume":"8 3","pages":"523-533"},"PeriodicalIF":0.0,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ame2.12556","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143257524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Targeting intracellular autophagic process for the treatment of post-stroke ischemia/reperfusion injury","authors":"Jun Hu,&nbsp;Zekai Hu,&nbsp;Jiayi Xia,&nbsp;Yeping Chen,&nbsp;Dennis Cordato,&nbsp;Qi Cheng,&nbsp;Jie Wang","doi":"10.1002/ame2.12528","DOIUrl":"10.1002/ame2.12528","url":null,"abstract":"<p>Cerebral ischemia/reperfusion (I/R) injury is an important pathophysiological condition of ischemic stroke that involves a variety of physiological and pathological cell death pathways, including autophagy, apoptosis, necroptosis, and phagoptosis, among which autophagy is the most studied. We have reviewed studies published in the past 5 years regarding the association between autophagy and cerebral I/R injury. To the best of our knowledge, this is the first review article summarizing potential candidates targeting autophagic pathways in the treatment of I/R injury post ischemic stroke. The findings of this review may help to better understand the pathogenesis and mechanisms of I/R events and bridge the gap between basic and translational research that may lead to the development of novel therapeutic approaches for I/R injury.</p>","PeriodicalId":93869,"journal":{"name":"Animal models and experimental medicine","volume":"8 3","pages":"389-404"},"PeriodicalIF":0.0,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ame2.12528","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143257532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Embarking on a new journey, exploring infinite possibilities—Welcoming the 2025 edition of Animal Models and Experimental Medicine","authors":"Chuan Qin","doi":"10.1002/ame2.12571","DOIUrl":"https://doi.org/10.1002/ame2.12571","url":null,"abstract":"&lt;p&gt;Against the backdrop of a new era that commands the attention of scholars worldwide, and on a scientific research journey filled with both hope and challenges, &lt;i&gt;Animal Models and Experimental Medicine&lt;/i&gt; (&lt;i&gt;AMEM&lt;/i&gt;), as a beacon guiding innovation and rigorous research in the field of animal models and experimental medicine, has once again shone brightly in 2024. With an impact factor of 3.8, a JCI and JCR Q2 ranking, and a CiteScore of 6.3, the journal has demonstrated a continuous rise in its influence and academic status.&lt;/p&gt;&lt;p&gt;In 2024, the number of submissions doubled to over 400, covering 59 countries and regions, with full-text views exceeding 350 000, spanning 216 countries and regions. Numerous manuscripts were cited by top international journals. To promote discipline development and exchange, we expanded content to 190 pages and organized six special issues covering cutting-edge fields such as stem cells, cardiovascular diseases, and bone regeneration. We not only provided a broad stage for groundbreaking research but also built an efficient, cutting-edge, and welfare-ethical academic exchange platform for scholars worldwide, facilitating international academic communication and dissemination of frontier knowledge.&lt;/p&gt;&lt;p&gt;Furthermore, we successfully convened an editorial board meeting, bringing together experts and scholars from 18 countries and regions to delve into issues such as journal development plans and optimization of peer review processes, aiming to better serve the scientific community. Based on this, we decided to change the publication frequency of the journal to monthly starting in 2025, providing scholars with more timely and comprehensive academic information through a more frequent publication cycle.&lt;/p&gt;&lt;p&gt;Notably, in response to critical medical health issues, we will launch a special issue on oncology in 2025, aiming to gather the latest research findings in the field of oncology from both domestic and international perspectives, providing robust scientific support for cancer prevention and treatment. Meanwhile, we will strengthen the study, promotion, and implementation of standards in the field of laboratory animals, enhancing the scientific and normative use of laboratory animals to safeguard the healthy development of experimental medicine.&lt;/p&gt;&lt;p&gt;Here, we also sincerely solicit ideas and contributions from practitioners in the field of laboratory animal science and technology and experimental medicine from around the world, covering articles on scientific research management, welfare ethics, and laboratory animal resources. We look forward to scholars sharing valuable experiences and resources to promote resource sharing and utilization, jointly advancing the progress and development of laboratory animal science and technology. Additionally, we will establish a special column to introduce outstanding research teams and leading enterprises, aiming to deepen industry exchanges. Professionals are welcome to ","PeriodicalId":93869,"journal":{"name":"Animal models and experimental medicine","volume":"8 1","pages":"3"},"PeriodicalIF":0.0,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ame2.12571","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143248811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study of fluoroquinolones resistance in rifampicin-resistant tuberculosis patients in Beijing: Characteristics, trends, and treatment outcomes","authors":"Wang Nenhan,&nbsp;Tian Lili,&nbsp;Zhao Yanfeng,&nbsp;Chen Shuangshuang,&nbsp;Tao LiYing,&nbsp;Li Qiao,&nbsp;Li Chuanyou,&nbsp;Dai Xiaowei","doi":"10.1002/ame2.12505","DOIUrl":"10.1002/ame2.12505","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>China is a high-burden country for multidrug-resistant tuberculosis/rifampin-resistant tuberculosis (MDR/RR-TB). Fluoroquinolones (FQs) are key drugs for the treatment of patients with MDR/RR-TB. However, research on the resistance of FQs in Beijing is limited.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>We collected clinical isolates from all patients with pulmonary TB in Beijing from January 2016 to December 2021, conducted drug-sensitivity tests and sequencing for levofloxacin (LFX) and moxifloxacin (MFX), and collected the treatment plans and outcomes of the patients.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>A total of 8512 clinical isolates were collected from patients with pulmonary TB, and 261 RR-TB strains were screened. The proportions of drug-sensitive and drug-resistant strains significantly differed by age group and treatment history. The rates of LFX and MFX resistance were 27.6% (72/261) and 36.4% (95/261), respectively. The detection rates of MDR-TB and pre-extensively drug-resistant TB (pre-XDR-TB) were 73.2% (191/261) and 36.4% (95/261), respectively, and the trends were significant (<i>χ</i>² trend = 9.995, <i>p</i> = 0.002; <i>χ</i>² trend = 12.744, <i>p</i> = 0.026). Among the 261 RR-TB strains, 14.9% (24/261) were sensitive to LFX but resistant to MFX. Among the four patients with LFX-resistant TB who received LFX treatment failed in three patients(Fisher's exact test, <i>p</i> = 0.009). The common mutation sites were 94 and 90 in gyrA. A novel mutation Ala90Ser was discovered.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>FQs resistance trends in RR-TB patients in Beijing are striking. Strains showed incomplete cross-resistance to LFX and MFX. Testing for FQs resistance and developing a reasonable treatment plan are recommended. Attention should be given to the changing trends in MDR-TB and pre-XDR-TB.</p>\u0000 </section>\u0000 </div>","PeriodicalId":93869,"journal":{"name":"Animal models and experimental medicine","volume":"8 5","pages":"906-915"},"PeriodicalIF":0.0,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ame2.12505","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143257530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The miR-6779/XIAP axis alleviates IL-1β-induced chondrocyte senescence and extracellular matrix loss in osteoarthritis","authors":"Zongchao Li,&nbsp;Aonan Dai,&nbsp;Xiaoxiang Fang,&nbsp;Kexing Tang,&nbsp;Kun Chen,&nbsp;Peng Gao,&nbsp;Jingyue Su,&nbsp;Xin Chen,&nbsp;Shengwu Yang,&nbsp;Zhenhan Deng,&nbsp;Liangjun Li","doi":"10.1002/ame2.12529","DOIUrl":"10.1002/ame2.12529","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Osteoarthritis (OA) is a long-term degenerative joint disease worsening over time. Aging and chondrocyte senescence contribute to OA progression. MicroRNAs have been confirmed to regulate different cellular processes. They contribute to OA pathology and may help to identify novel biomarkers and therapies for OA.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>This study used bioinformatics and experimental investigations to analyze and validate differentially expressed miRNAs in OA that might affect chondrocyte apoptosis and senescence.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>miR-6779 was found to be significantly down-regulated in OA. Seventy-six of the predicted and miR-6779 targeted genes and the OA-associated disease genes overlapped, and these were enriched in cell proliferation, cell apoptosis, and cell cycle. miR-6779 overexpression remarkably attenuated IL-1β effects on chondrocytes by reducing MMP3 and MMP13 levels, promoting cell apoptosis, suppressing cell senescence, and increasing caspase-3, caspase-9 and reducing P16 and P21 levels. miR-6779 targeted inhibition of X-linked inhibitor of apoptosis protein (XIAP) expression. XIAP knockdown partially improved IL-1β-induced chondrocyte senescence and dysfunction. Lastly, when co-transfected with a miR-6779 agomir, the XIAP overexpression vector partially attenuated the effects of miR-6779 overexpression on chondrocytes; miR-6779 improved IL-1β-induced senescence and dysfunction in chondrocytes through targeting XIAP.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>miR-6779 is down-regulated, and XIAP is up-regulated in OA cartilage and IL-1β-treated chondrocytes. miR-6779 inhibits XIAP expression, thereby promoting senescent chondrocyte cell apoptosis and reducing chondrocyte senescence and ECM loss through XIAP.</p>\u0000 </section>\u0000 </div>","PeriodicalId":93869,"journal":{"name":"Animal models and experimental medicine","volume":"8 4","pages":"662-673"},"PeriodicalIF":0.0,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ame2.12529","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143191613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pharmacokinetic analysis on compatibility of Atractylodes macrocephala and Paeoniae radix herb pair ameliorates functional constipation model rats using microdialysis with ultra-performance liquid chromatography","authors":"Xiaoting Wang,&nbsp;Xiaojun Li,&nbsp;Rui Zhang,&nbsp;Yin Hong,&nbsp;Jiaqi Guan","doi":"10.1002/ame2.12550","DOIUrl":"10.1002/ame2.12550","url":null,"abstract":"&lt;div&gt;\u0000 \u0000 \u0000 &lt;section&gt;\u0000 \u0000 &lt;h3&gt; Background&lt;/h3&gt;\u0000 \u0000 &lt;p&gt;In a previous study, we found that Atractylodes macrocephala and Paeoniae radix (AM-PR) was useful for the alleviation of functional constipation (FC). However, the precise mechanism underlying the compatibility between AM and PR in the treatment of FC remains uncertain. This study aims to analyze the pharmacokinetic differences in the active ingredients in the blood of rat models with FC when applied individually and in combination with AM-PR. It also seeks to compare the changes in the content of the active ingredient when applied individually and in combination with in vitro AM-PR, further in-depth investigation into its material foundation in terms of pharmacokinetics, as well as the composition of the substance.&lt;/p&gt;\u0000 &lt;/section&gt;\u0000 \u0000 &lt;section&gt;\u0000 \u0000 &lt;h3&gt; Methods&lt;/h3&gt;\u0000 \u0000 &lt;p&gt;Blood microdialysis samples were collected using microdialysis technology. These samples from rats with FC were compared after administration of AM, PR, and AM-PR. The concentration of the main active ingredients was determined using the Ultra Performance Liquid Chromatography-Tunable Ultraviolet (UPLC-TUV) method. The concentration of the main active ingredients of the decoction compatibility before and after combining AM-PR was also determined using the UPLC-TUV method.&lt;/p&gt;\u0000 &lt;/section&gt;\u0000 \u0000 &lt;section&gt;\u0000 \u0000 &lt;h3&gt; Results&lt;/h3&gt;\u0000 \u0000 &lt;p&gt;Our findings reveal that upon combination, the time to maximum concentration (&lt;i&gt;T&lt;/i&gt;&lt;sub&gt;max&lt;/sub&gt;) of isochlorogenic acid A (ICA-A) and ataridolide II (ATR-II) &lt;i&gt;T&lt;/i&gt;&lt;sub&gt;max&lt;/sub&gt; was prolonged, terminal elimination half-life (&lt;i&gt;T&lt;/i&gt;&lt;sub&gt;1/2&lt;/sub&gt;) was reduced, and maximum plasma concentrations (&lt;i&gt;C&lt;/i&gt;&lt;sub&gt;max&lt;/sub&gt;) increased. The &lt;i&gt;T&lt;/i&gt;&lt;sub&gt;max&lt;/sub&gt; of ataridolide III (ATR-III) remained consistent, whereas its &lt;i&gt;T&lt;/i&gt;&lt;sub&gt;1/2&lt;/sub&gt; and C&lt;sub&gt;max&lt;/sub&gt; were significantly reduced. Conversely, for peoniflorin (PAE), &lt;i&gt;T&lt;/i&gt;&lt;sub&gt;max&lt;/sub&gt; occurred sooner, &lt;i&gt;T&lt;/i&gt;&lt;sub&gt;1/2&lt;/sub&gt; was shortened, and &lt;i&gt;C&lt;/i&gt;&lt;sub&gt;max&lt;/sub&gt; increased. The &lt;i&gt;T&lt;/i&gt;&lt;sub&gt;max&lt;/sub&gt; for albiflorin (ALB) remained consistent, whereas &lt;i&gt;T&lt;/i&gt;&lt;sub&gt;1/2&lt;/sub&gt; and &lt;i&gt;C&lt;/i&gt;&lt;sub&gt;max&lt;/sub&gt; witnessed significant increases. The area under the moment curve (AUMC) (0–&lt;i&gt;t&lt;/i&gt;) and AUMC (0–∞) of PAE, ALB, ICA-A, ATR-II experienced an increase after AM-PR administration in rats, attributable to the heightened &lt;i&gt;C&lt;/i&gt;&lt;sub&gt;max&lt;/sub&gt;. In comparison to individual herb administration, the &lt;i&gt;T&lt;/i&gt;&lt;sub&gt;max&lt;/sub&gt; of ALB was advanced in combination, the &lt;i&gt;T&lt;/i&gt;&lt;sub&gt;max&lt;/sub&gt; of PAE remained unchanged, and the &lt;i&gt;T&lt;/i&gt;&lt;sub&gt;max&lt;/sub&gt; of ICA-A and ART-II was delayed, with an increased area under the concentration–time curve (AUC) (0–&lt;i&gt;t&lt;/i&gt;), indicating enhanced &lt;i&gt;C&lt;/i&gt;&lt;sub","PeriodicalId":93869,"journal":{"name":"Animal models and experimental medicine","volume":"8 5","pages":"874-885"},"PeriodicalIF":0.0,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ame2.12550","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143048657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"HC-A solution limb perfusion alleviates liver damage induced by limb ischemia–reperfusion injury in pigs","authors":"Bowen Zhang,&nbsp;Weixiang Ni,&nbsp;Qinglong Cai,&nbsp;Huitao Ji,&nbsp;Junhao Du,&nbsp;Weixuan Hong,&nbsp;Junwei Fang,&nbsp;Lie Wang,&nbsp;Yafeng Qi,&nbsp;Chunhong Xiao","doi":"10.1002/ame2.12517","DOIUrl":"10.1002/ame2.12517","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>The aim of the study was to explore a feasible method for alleviating limb ischemia–reperfusion injury (LI/RI) through the use of a high-concentration citrate solution (HC-A solution) for limb perfusion (LP).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Eighteen pigs were divided into three groups: the Sham group, LI/RI group, and HCA group. The Sham group underwent exposure of the iliac artery and vein. The LI/RI group underwent tourniquet placement and clamping of the iliac artery and vein to simulate LI/RI. The HCA group received HC-A solution LP for 30 min through the left iliac artery below the level of blood flow occlusion based on the LI/RI group. Oxidative stress markers and inflammatory response markers were compared among the three groups.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Compared to the LI/RI group, the HCA group showed significantly lower levels of serum creatine kinase (CK), lactate dehydrogenase (LDH), malondialdehyde (MDA), tumor necrosis factor-α (TNF-α), aspartate aminotransferase (AST), and alanine aminotransferase (ALT), and significantly greater activities of serum superoxide dismutase (SOD) (<i>p</i> &lt; 0.05). There were no significant differences in serum interleukin-6 (IL-6) or in muscle MDA, SOD, TNF-α, and IL-6 between the HCA group and the LI/RI group (<i>p</i> &gt; 0.05). Compared to the LI/RI group, MDA, TNF-α, and IL-6 levels in the liver were significantly lower in the HCA group (<i>p</i> &lt; 0.05), while SOD activities were not significantly different (<i>p</i> &gt; 0.05). Histopathological examination revealed reduced skeletal muscle and liver damage in the HCA group compared to the LI/RI group.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>HC-A solution LP can alleviate liver damage caused by LI/RI in pigs.</p>\u0000 </section>\u0000 </div>","PeriodicalId":93869,"journal":{"name":"Animal models and experimental medicine","volume":"8 3","pages":"421-428"},"PeriodicalIF":0.0,"publicationDate":"2025-01-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ame2.12517","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143048723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antiviral effects and mechanism of Qi pi pill against influenza viruses.","authors":"Chengcheng Zhang, Jing Gao, Meiyue Dong, Carolina Q Sacramento, Ping Li, Xiangyu Lian, Lingyuan Fan, Lijun Rong, Ruikun Du, Jingzhen Tian, Qinghua Cui","doi":"10.1002/ame2.12511","DOIUrl":"https://doi.org/10.1002/ame2.12511","url":null,"abstract":"<p><strong>Background: </strong>Qi pi pill (QPP), which contains Renshen, Baizhu, Fuling, Gancao, Chenpi, Shanyao, Lianzi, Shanzha, Liushenqu, Maiya, and Zexie, was recommended for preventing and treating COVID-19 in Shandong Province (China). However, the mechanism by which QPP treats infectious diseases remains unclear. This study aims to investigate the therapeutic effect of QPP in vitro and on acute influenza infection in mice, exploring its mechanism of action against influenza A virus (IAV).</p><p><strong>Methods: </strong>The in vitro activity of QPP was assessed using dose-response curve analysis and titer reduction assay, and its antiviral mechanism was identified in vitro by real-time polymerase chain reaction (PCR), time-of-addition, and enzymatic assays. The antiviral efficacy of QPP was further evaluated in vivo using BALB/c mice infected with IAV. At the same time, each single Chinese herbal medicine in QPP was evaluated to preliminarily identify those with antiviral effects.</p><p><strong>Results: </strong>In vitro results showed that QPP exhibited a higher potency antiviral effect against both influenza A and B viruses, inhibiting viral RNA replication and release by targeting RNA-dependent RNA polymerase and neuraminidase. Additionally, QPP significantly decreased the expression of inflammatory cytokines in A549 cells. In vivo study revealed that QPP significantly reduced the lung index and viral load in lung tissue of mice infected with IAV. Renshen, Gancao, Zexie, and Lianzi were the Chinese herbal medicines from QPP that showed anti-IAV activity.</p><p><strong>Conclusion: </strong>The antiviral activity of QPP targets IAV replication and release, cytokine modulation in host cells, and provides protection in mice with acute influenza infection.</p>","PeriodicalId":93869,"journal":{"name":"Animal models and experimental medicine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-01-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143048613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}