HC-A solution limb perfusion alleviates liver damage induced by limb ischemia–reperfusion injury in pigs

IF 3.4 Q1 Health Professions

Animal models and experimental medicine Pub Date : 2025-01-26 DOI:10.1002/ame2.12517

Bowen Zhang, Weixiang Ni, Qinglong Cai, Huitao Ji, Junhao Du, Weixuan Hong, Junwei Fang, Lie Wang, Yafeng Qi, Chunhong Xiao

{"title":"HC-A solution limb perfusion alleviates liver damage induced by limb ischemia–reperfusion injury in pigs","authors":"Bowen Zhang, Weixiang Ni, Qinglong Cai, Huitao Ji, Junhao Du, Weixuan Hong, Junwei Fang, Lie Wang, Yafeng Qi, Chunhong Xiao","doi":"10.1002/ame2.12517","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Background</h3>\n \n <p>The aim of the study was to explore a feasible method for alleviating limb ischemia–reperfusion injury (LI/RI) through the use of a high-concentration citrate solution (HC-A solution) for limb perfusion (LP).</p>\n </section>\n \n <section>\n \n <h3> Methods</h3>\n \n <p>Eighteen pigs were divided into three groups: the Sham group, LI/RI group, and HCA group. The Sham group underwent exposure of the iliac artery and vein. The LI/RI group underwent tourniquet placement and clamping of the iliac artery and vein to simulate LI/RI. The HCA group received HC-A solution LP for 30 min through the left iliac artery below the level of blood flow occlusion based on the LI/RI group. Oxidative stress markers and inflammatory response markers were compared among the three groups.</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>Compared to the LI/RI group, the HCA group showed significantly lower levels of serum creatine kinase (CK), lactate dehydrogenase (LDH), malondialdehyde (MDA), tumor necrosis factor-α (TNF-α), aspartate aminotransferase (AST), and alanine aminotransferase (ALT), and significantly greater activities of serum superoxide dismutase (SOD) (<i>p</i> < 0.05). There were no significant differences in serum interleukin-6 (IL-6) or in muscle MDA, SOD, TNF-α, and IL-6 between the HCA group and the LI/RI group (<i>p</i> > 0.05). Compared to the LI/RI group, MDA, TNF-α, and IL-6 levels in the liver were significantly lower in the HCA group (<i>p</i> < 0.05), while SOD activities were not significantly different (<i>p</i> > 0.05). Histopathological examination revealed reduced skeletal muscle and liver damage in the HCA group compared to the LI/RI group.</p>\n </section>\n \n <section>\n \n <h3> Conclusions</h3>\n \n <p>HC-A solution LP can alleviate liver damage caused by LI/RI in pigs.</p>\n </section>\n </div>","PeriodicalId":93869,"journal":{"name":"Animal models and experimental medicine","volume":"8 3","pages":"421-428"},"PeriodicalIF":3.4000,"publicationDate":"2025-01-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/ame2.12517","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Animal models and experimental medicine","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/ame2.12517","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"Health Professions","Score":null,"Total":0}

引用次数: 0

Abstract

Background

The aim of the study was to explore a feasible method for alleviating limb ischemia–reperfusion injury (LI/RI) through the use of a high-concentration citrate solution (HC-A solution) for limb perfusion (LP).

Methods

Eighteen pigs were divided into three groups: the Sham group, LI/RI group, and HCA group. The Sham group underwent exposure of the iliac artery and vein. The LI/RI group underwent tourniquet placement and clamping of the iliac artery and vein to simulate LI/RI. The HCA group received HC-A solution LP for 30 min through the left iliac artery below the level of blood flow occlusion based on the LI/RI group. Oxidative stress markers and inflammatory response markers were compared among the three groups.

Results

Compared to the LI/RI group, the HCA group showed significantly lower levels of serum creatine kinase (CK), lactate dehydrogenase (LDH), malondialdehyde (MDA), tumor necrosis factor-α (TNF-α), aspartate aminotransferase (AST), and alanine aminotransferase (ALT), and significantly greater activities of serum superoxide dismutase (SOD) (p < 0.05). There were no significant differences in serum interleukin-6 (IL-6) or in muscle MDA, SOD, TNF-α, and IL-6 between the HCA group and the LI/RI group (p > 0.05). Compared to the LI/RI group, MDA, TNF-α, and IL-6 levels in the liver were significantly lower in the HCA group (p < 0.05), while SOD activities were not significantly different (p > 0.05). Histopathological examination revealed reduced skeletal muscle and liver damage in the HCA group compared to the LI/RI group.

Conclusions

HC-A solution LP can alleviate liver damage caused by LI/RI in pigs.

Abstract Image

查看原文本刊更多论文

HC-A溶液肢体灌注可减轻猪肢体缺血再灌注损伤引起的肝损伤。

背景：本研究旨在探讨高浓度柠檬酸溶液（HC-A溶液）用于肢体灌注（LP）减轻肢体缺血再灌注损伤（LI/RI）的可行方法。方法：18头猪随机分为Sham组、LI/RI组和HCA组。假手术组行髂动静脉暴露术。LI/RI组通过放置止血带和夹紧髂动静脉来模拟LI/RI。HCA组在LI/RI组的基础上，在血流闭塞水平以下通过左髂动脉给予HC-A溶液LP 30 min。比较三组小鼠氧化应激指标和炎症反应指标。结果：与LI/RI组相比，HCA组血清肌酸激酶（CK）、乳酸脱氢酶（LDH）、丙二醛（MDA）、肿瘤坏死因子-α (TNF-α)、天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）水平显著降低（p < 0.05），血清超氧化物歧化酶（SOD）活性显著升高（p < 0.05）。与LI/RI组比较，HCA组肝脏组织中MDA、TNF-α、IL-6水平显著降低（p 0.05）。组织病理学检查显示，与LI/RI组相比，HCA组骨骼肌和肝脏损伤减少。结论：HC-A LP溶液可减轻LI/RI对猪肝脏的损害。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊