ACS ES&T water最新文献_第7页

Field Evaluation of pBI143 Genetic Marker for Microbial Source Tracking in Urban Surface Waters pBI143遗传标记在城市地表水微生物源追踪中的现场评价

IF 4.5

ACS ES&T water Pub Date : 2026-09-11 Epub Date: 2026-08-25 DOI: 10.1021/acsestwater.6c00364

Huimin Zhang, Ocean Thakali, Sajina Paudel, Mustafa Ali, Abhilasha Shrestha, Metasebia Gebrewold, Leah Clarke, Warish Ahmed, Samendra P. Sherchan

{"title":"Field Evaluation of pBI143 Genetic Marker for Microbial Source Tracking in Urban Surface Waters","authors":"Huimin Zhang, Ocean Thakali, Sajina Paudel, Mustafa Ali, Abhilasha Shrestha, Metasebia Gebrewold, Leah Clarke, Warish Ahmed, Samendra P. Sherchan","doi":"10.1021/acsestwater.6c00364","DOIUrl":"https://doi.org/10.1021/acsestwater.6c00364","url":null,"abstract":"This study evaluated the sewage-associated plasmid pBI143usingtwo independent surface water data sets: long-term monitoring fromLake Pontchartrain, New Orleans (2016–2017), and short-termurban sampling at Chicago beaches (2025). pBI143 was compared withsewage-associated Carjivirus (formerlycrAssphage) and, where available, enterococci, turbidity, and humanadenovirus (HAdV). Conditional probability analysis was used to estimateconfidence that sewage pollution was present given pBI143 detection.In New Orleans, pBI143 was detected in 88.3% of samples (53/60), comparedwith 35.0% (21/60) of Carjivirus. All Carjivirus-positive samples werealso pBI143-positive. In Chicago, pBI143 and Carjivirus were detected in 66.7% (24/36) and 52.8% (19/36) of samples, respectively,with partial overlap. pBI143 was positively correlated with Carjivirus in New Orleans (Spearman ρ = 0.545, p < 0.0001), whereas the association was weaker in Chicago(ρ = 0.332, p = 0.048). Agreement analysisindicated weak to moderate concordance between pBI143 and Carjivirus in both waters, emphasizing marker complementarity.Conditional probability analysis indicated high confidence of sewagepollution given pBI143 detection, with posterior probabilities of98.4–99.1% in New Orleans and 94.3–96.6% in Chicago.These results indicate that pBI143 is a sensitive sewage-associatedmarker that complements Carjivirus forMST in surface waters.","PeriodicalId":93847,"journal":{"name":"ACS ES&T water","volume":"6 9","pages":"5750–5759"},"PeriodicalIF":4.5,"publicationDate":"2026-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148950645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Copper Ion Removal Method and Engineering Dosing Prediction Model Based on Chlorella–Actinomycetes Symbiotic System 基于小球藻-放线菌共生系统的铜离子去除方法及工程投加预测模型

IF 4.5

ACS ES&T water Pub Date : 2026-09-11 Epub Date: 2026-08-10 DOI: 10.1021/acsestwater.6c00303

Ying Chen, Shengyuan Hu, Ziyi Li, Yifeng Wu, Dandan Zhu, Qiguang Zhu

{"title":"Copper Ion Removal Method and Engineering Dosing Prediction Model Based on Chlorella–Actinomycetes Symbiotic System","authors":"Ying Chen, Shengyuan Hu, Ziyi Li, Yifeng Wu, Dandan Zhu, Qiguang Zhu","doi":"10.1021/acsestwater.6c00303","DOIUrl":"https://doi.org/10.1021/acsestwater.6c00303","url":null,"abstract":"To address the lack of systematic ratio-screening logicand engineeringdecision-making basis in existing microalgae-bacteria symbiotic systemsfor heavy metal wastewater treatment, the biologicalremoval systems were constructed using different ratios of Chlorella sp. and Streptomyces sp. to treat Cu2+-containing wastewater in this research.Cu2+ concentrations were determined by DDTC colorimetricspectrophotometry to calculate removal efficiency, and the optimalend point time for Cu2+ removal was investigated underdifferent algae-bacteria ratios. The results showed that, within theadvantageous region of \"more algae, less bacteria\", theoptimal operableinoculation volume ratio of Chlorella sp. to Streptomyces sp. was furtheridentified as 3:1. On this basis, a predictive model based on theparticle swarm optimization-support vector regression (PSO-SVR) algorithmwas introduced to solve the engineering dosing volume problems, inwhich the initial concentration and target compliance days were regardedas the inputs and the required dosing volume as the output. The resultsdemonstrated that the model exhibited good generalization performance(R2 = 0.9025) and was further transformedinto a visualized \"concentration–time frame-dosage\"decisionheatmap. This study confirmed the synergistic advantage of the Chlorella/Streptomyces system in Cu2+ removaland provided visual references and model support for compliance-baseddosing decisions.","PeriodicalId":93847,"journal":{"name":"ACS ES&T water","volume":"6 9","pages":"5723–5733"},"PeriodicalIF":4.5,"publicationDate":"2026-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148950884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

How Are Micronanoplastics (MNPs) Collected and Identified in Aquatic Environments? A Pathway Review from Sampling to Spectral─Artificial Intelligence 如何在水生环境中收集和鉴定微塑料（MNPs）？从采样到光谱的路径回顾──人工智能

IF 4.5

ACS ES&T water Pub Date : 2026-09-11 Epub Date: 2026-07-29 DOI: 10.1021/acsestwater.6c00708

Xue Rong, Yitong Han, Lianzhen Li, Yanhao Wang, Xilin She

{"title":"How Are Micronanoplastics (MNPs) Collected and Identified in Aquatic Environments? A Pathway Review from Sampling to Spectral─Artificial Intelligence","authors":"Xue Rong, Yitong Han, Lianzhen Li, Yanhao Wang, Xilin She","doi":"10.1021/acsestwater.6c00708","DOIUrl":"https://doi.org/10.1021/acsestwater.6c00708","url":null,"abstract":"Theextensive use of plastic products has established micronanoplastics(MNPs) as a critical emerging contaminant in the global water environment,posing significant threats to ecological integrity and human health.This review synthesizes current methodologies for sampling, pretreatment,and identification of MNPs in water environments and evaluates theirrespective advantages and limitations. Spectroscopic techniques, massspectrometry, chromatography, thermal analysis, and scanning electronmicroscopy form the core analytical toolkit for MNP identification.Each method exhibits distinct strengths in its sensitivity, spatialresolution, and applicability. Additionally, we analyzed the applicationof artificial intelligence (AI) to microplastic sampling and identification.Researchers believe that integrating AI can significantly enhancecollection efficiency and improve the accuracy of spectral analysisin sample identification. Future studies should further advance thedevelopment of AI in MNP research to facilitate accurate classificationand risk assessment in aquatic systems.","PeriodicalId":93847,"journal":{"name":"ACS ES&T water","volume":"6 9","pages":"5566–5583"},"PeriodicalIF":4.5,"publicationDate":"2026-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148950926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unveiling the Dual-Coupling Mechanism of Pipe Bursts: Integrating Transient Hydraulics, Geometric Stress, and Adaptive Parallel Computing 揭示管道爆裂的双重耦合机制：整合瞬态水力、几何应力和自适应并行计算

IF 4.5

ACS ES&T water Pub Date : 2026-09-11 Epub Date: 2026-06-25 DOI: 10.1021/acsestwater.6c00491

Feiyu Duan, Tianwei Mu, Bo Zhou, Haoqiang Tan, Manhong Huang

{"title":"Unveiling the Dual-Coupling Mechanism of Pipe Bursts: Integrating Transient Hydraulics, Geometric Stress, and Adaptive Parallel Computing","authors":"Feiyu Duan, Tianwei Mu, Bo Zhou, Haoqiang Tan, Manhong Huang","doi":"10.1021/acsestwater.6c00491","DOIUrl":"https://doi.org/10.1021/acsestwater.6c00491","url":null,"abstract":"Pipe burst risk assessment is frequently hindered bythe inabilityto capture nonlinear interactions between transient hydraulics andcomplex network geometries. This disconnect often leaves failuresin ostensibly safe low-pressure zones unexplained. This study proposesa high-fidelity Computational-Geometric-Physical framework to resolvethis challenge. First, to overcome computational bottlenecks, we presenta Topology-Adaptive Spectral Partitioning and Geometric Extraction(TAS-PGE) that accelerates large-scale simulations by 1.51×.This efficiency enables our core innovation: the Geometric-HydraulicDual Coupling Model (GH-DCSIM). It reveals a critical mechanism wheretransient waves and acute bends amplify stress far beyond static predictions.Crucially, we prove that traditional static methods underestimaterisks in U-bends by 2.9-fold (Safety Factor 2.00 vs 0.68), misidentifyingimminent failures as safe. Finally, a variable wave speed mechanismincorporating gas–liquid interaction eliminates physical distortions.To establish physical applicability, we constructed a comprehensiveLiterature Benchmark Database, comprising real-world accident reconstructions,design limit states, and synthetic parametric scenarios. Independentvalidation on 34 real-world cases confirms the framework’srobustness, with the mean burst-risk ratio significantly exceedingthe failure limit of 1.0 (mean R = 2.80; 95% CI:[1.847, 3.911]). This work shifts the paradigm from pressure-dominatedassessment to multiphysics coupling, providing a rigorous basis forpredictive maintenance.","PeriodicalId":93847,"journal":{"name":"ACS ES&T water","volume":"6 9","pages":"5885–5897"},"PeriodicalIF":4.5,"publicationDate":"2026-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148950725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Statewide Assessment of TFA, PFAS, and AOF in Alabama Surface Waters: Influence of Precipitation and Wastewater Effluent 阿拉巴马州地表水中TFA、PFAS和AOF的全州评估：降水和废水排放的影响

IF 4.5

ACS ES&T water Pub Date : 2026-09-11 Epub Date: 2026-08-29 DOI: 10.1021/acsestwater.6c00546

Sarah K. Ortbal, Md Abdullah Al Masud, Mahlet Melaku Kebede, Aaron Rak, Leigh G. Terry

{"title":"Statewide Assessment of TFA, PFAS, and AOF in Alabama Surface Waters: Influence of Precipitation and Wastewater Effluent","authors":"Sarah K. Ortbal, Md Abdullah Al Masud, Mahlet Melaku Kebede, Aaron Rak, Leigh G. Terry","doi":"10.1021/acsestwater.6c00546","DOIUrl":"https://doi.org/10.1021/acsestwater.6c00546","url":null,"abstract":"Per- and polyfluoroalkylsubstances (PFAS), a diverseclass ofsynthetic organic chemicals, are widely detected in wastewater treatmentplant (WWTP) effluents and receiving surface waters. This study evaluated40 PFAS, including trifluoroacetic acid (TFA), and adsorbable organicfluorine (AOF) in surface waters upstream and downstream of WWTPs,as well as in lakes across Alabama, United States. No significantdifferences were observed between upstream and downstream samples,indicating limited influence of PFAS in WWTP effluent on receivingwaters relative to overall streamflow. Samples collected followingprecipitation ≥2.5 mm exhibited significantly lower PFOA, PFOS,and Σ39 PFAS concentrations due to contaminant dilution. Incontrast, samples collected following precipitation ≥2.5 mmexhibited significantly higher TFA and AOF concentrations, highlightingprecipitation-driven inputs as a source pathway. Spearman correlationwas conducted to relate PFAS concentrations with anthropogenic data.TFA was frequently detected at high concentrations (mean: 773.0 ng/L).The majority of organofluorine in surface waters (96.4%) was not capturedby PFAS measured using EPA Method 1633. These findings demonstratethe widespread occurrence of PFAS, including TFA, and unidentifiedorganofluorine in Alabama surface waters and underscore the importanceof integrating targeted and bulk organofluorine measurements to bettercharacterize contaminant burdens and source pathways.","PeriodicalId":93847,"journal":{"name":"ACS ES&T water","volume":"6 9","pages":"5918–5931"},"PeriodicalIF":4.5,"publicationDate":"2026-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148950934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction of “Polyhalogenated Carbazoles in Sediments of Lower Laurentian Great Lakes and Regional Perspectives” “下劳伦森五大湖沉积物中多卤代咔唑的校正及区域展望”

IF 4.5

ACS ES&T water Pub Date : 2026-09-11 Epub Date: 2026-08-26 DOI: 10.1021/acsestwater.6c01100

An Li, Shanshan Zhou, Huan He, Jiehong Guo, Karl J. Rockne, Neil C. Sturchio, John P. Giesy

引用次数: 0

Highly Efficient Removal of Phosphate by Film-like Purifier Prepared via Dopamine-Assisted Fixation of Lanthanum Hydroxide onto Bacterial Cellulose 用多巴胺辅助氢氧化镧固定细菌纤维素制备的膜状净化器高效去除磷酸盐

IF 4.5

ACS ES&T water Pub Date : 2026-09-11 Epub Date: 2026-08-12 DOI: 10.1021/acsestwater.5c01288

Yuqian Jia, Tian Wang, Fiona Zhu, Youzhu Pan, Minggui Yu, Yanyang Zhang, Fei Liu, Liuguan Ding, Jieshu Qian

{"title":"Highly Efficient Removal of Phosphate by Film-like Purifier Prepared via Dopamine-Assisted Fixation of Lanthanum Hydroxide onto Bacterial Cellulose","authors":"Yuqian Jia, Tian Wang, Fiona Zhu, Youzhu Pan, Minggui Yu, Yanyang Zhang, Fei Liu, Liuguan Ding, Jieshu Qian","doi":"10.1021/acsestwater.5c01288","DOIUrl":"https://doi.org/10.1021/acsestwater.5c01288","url":null,"abstract":"Eutrophication caused by excess phosphate is a growingthreat toaquatic ecosystems, driving the need for efficient and practical removaltechnologies. Lanthanum (La)-based adsorbents are of significant interestdue to their high affinity for phosphate, structural tunability, andefficient metal utilization. In this study, we developed a flexible,film-like composite adsorbent by uniformly anchoring La(OH)3 nanoparticles onto a 3D bacterial cellulose nanofiber network usinga dopamine-assisted approach. The resulting composite achieves nearlycomplete phosphate removal (∼100%) at concentrations below10 mg/L, while retaining high efficiency over a broad pH range andin complex aqueous conditions. Notably, it also exhibits exceptionalLa utilization and a phosphate adsorption capacity higher than mostpreviously reported powder-based adsorbents. The flexible film-likearchitecture offers the critical practical advantages of easy handlingand separation from treated water. This work demonstrates a rationalintegration strategy for fabricating a flexible film adsorbent thatachieves high La utilization efficiency and practical operabilityfor targeted low-concentration phosphate removal in water treatment.","PeriodicalId":93847,"journal":{"name":"ACS ES&T water","volume":"6 9","pages":"5650–5659"},"PeriodicalIF":4.5,"publicationDate":"2026-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148950621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification and Quantification of a Predicted Iodinated Disinfection Byproduct in Chlorinated Waters–Dichloroiodoacetic Acid 氯化水中可预测的碘消毒副产物二氯碘乙酸的鉴定与定量

IF 4.5

ACS ES&T water Pub Date : 2026-09-11 Epub Date: 2026-08-20 DOI: 10.1021/acsestwater.6c00463

Jinbo Chen, Shinya Echigo, Kai He, Yuto Tada, Klon D. C. Hinneh, Sadahiko Itoh

{"title":"Identification and Quantification of a Predicted Iodinated Disinfection Byproduct in Chlorinated Waters–Dichloroiodoacetic Acid","authors":"Jinbo Chen, Shinya Echigo, Kai He, Yuto Tada, Klon D. C. Hinneh, Sadahiko Itoh","doi":"10.1021/acsestwater.6c00463","DOIUrl":"https://doi.org/10.1021/acsestwater.6c00463","url":null,"abstract":"Identifyingunknown iodinated disinfection byproducts(I-DBPs)is critical for drinking water safety because of their high toxicity.This study identified and quantified a previously predicted yet analyticallychallenging I-DBP, dichloroiodoacetic acid (DCIAA), which was overlookedduring electrospray ionization because of in-source fragmentation.The molecular formula and structure of DCIAA were confirmed by usinghigh-resolution mass spectrometry full-scan analysis and product ionscan characterization of the chlorinated products of the organic iodine-containingprecursors. After isolating and purifying DCIAA generated throughthe chlorination of 2-iodophenol at pH 8.0, the compound was quantifiedusing inductively coupled plasma mass spectrometry and subsequentlyused as a standard. DCIAA was stored in a 10 mM phosphate buffer solution(pH 7.0) and remained stable under simulated drinking water distributionsystem conditions. The quantification method was developed using multiplereaction monitoring with optimized precursor/product ion transitionsat m/z 208.8/126.9. Weak anion-exchangecartridges exhibited optimal recovery for the solid-phase extractionof DCIAA. DCIAA concentrations ranged from undetectable to 38.8 ng/Lin real tap water samples in Japan and from 129.8 to 463.1 ng/L inchlorinated raw water prepared in the laboratory, revealing the occurrenceand importance of DCIAA in drinking water.","PeriodicalId":93847,"journal":{"name":"ACS ES&T water","volume":"6 9","pages":"5829–5838"},"PeriodicalIF":4.5,"publicationDate":"2026-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148950780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Driving Factors of Macroplastic Dynamics in Rivers: A Global Review 河流宏观塑性动力学的驱动因素：全球综述

IF 4.5

ACS ES&T water Pub Date : 2026-09-11 Epub Date: 2026-08-25 DOI: 10.1021/acsestwater.6c00608

Rose Boahemaa Pinto, Tim H. M. van Emmerik, Martine van der Ploeg, Giulia Cesarini

{"title":"Driving Factors of Macroplastic Dynamics in Rivers: A Global Review","authors":"Rose Boahemaa Pinto, Tim H. M. van Emmerik, Martine van der Ploeg, Giulia Cesarini","doi":"10.1021/acsestwater.6c00608","DOIUrl":"https://doi.org/10.1021/acsestwater.6c00608","url":null,"abstract":"Understanding riverine macroplastic dynamics is critical,as riversare the main pathways transferring land-based plastics to the ocean,contributing to global marine pollution. This review synthesizes currentresearch on the environmental factors controlling macroplastic dynamicsin rivers, including hydrometeorological (e.g., rainfall, discharge),anthropogenic (e.g., mismanaged plastic waste, population density),and river characteristics (geographical features, e.g., riparian vegetation,slope, and man-made, e.g., dam, weir). A systematic literature searchidentified 193 studies, which were classified into ten relationshiptypes for cross-study comparison. Our analysis reveals a strong researchbias toward hydrometeorological drivers (53.7%), with fewer studiesaddressing anthropogenic or river characteristics (23.1% each). Environmentaldrivers are investigated unevenly across macroplastic transport phases,with hydrometeorological variables primarily linked to movement, anthropogenicfactors to input, and river characteristics to both movement and accumulation.However, these relationships remain inconsistent across studies dueto methodological and environmental differences. Importantly, a largeproportion of studies rely on qualitative or descriptive approaches,with limited use of statistical testing to quantitatively evaluatedriver–transport relationships. Geographic imbalances werealso evident, with most research focused on Europe and Asia. Thisreview highlights the need for more quantitative, evidence-based researchto establish globally applicable links between environmental factorsand riverine macroplastic pollution.","PeriodicalId":93847,"journal":{"name":"ACS ES&T water","volume":"6 9","pages":"5538–5552"},"PeriodicalIF":4.5,"publicationDate":"2026-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148950682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Framework for Developing a Simple Method of Analyzing Environmental Bacterial Cells: Combining Single-Round DNA Aptamer Selection with Gold Nanoparticle Sensor Development for Legionella pneumophila Detection 开发一种简单的环境细菌细胞分析方法的框架：将单轮DNA适体选择与金纳米颗粒传感器开发相结合用于嗜肺军团菌检测

IF 4.5

ACS ES&T water Pub Date : 2026-09-11 Epub Date: 2026-08-12 DOI: 10.1021/acsestwater.6c00237

Koji Matsunaga, Yuiko Tasaki-Handa, Yota Suzuki, Taihei Murakami, Kanji Nakao, Hisashi Satoh, Shingo Saito

{"title":"Framework for Developing a Simple Method of Analyzing Environmental Bacterial Cells: Combining Single-Round DNA Aptamer Selection with Gold Nanoparticle Sensor Development for Legionella pneumophila Detection","authors":"Koji Matsunaga, Yuiko Tasaki-Handa, Yota Suzuki, Taihei Murakami, Kanji Nakao, Hisashi Satoh, Shingo Saito","doi":"10.1021/acsestwater.6c00237","DOIUrl":"https://doi.org/10.1021/acsestwater.6c00237","url":null,"abstract":"Monitoring pathogenic microorganisms in public and environmentalwaters is important for protecting ecosystems and human health; however,current detection methods are expensive, labor-intensive, and time-consuming.Although DNA aptasensors can potentially overcome these limitations,conventional aptamer selections often fail to secure high-affinityaptamer sequences, besides being complicated and lengthy. In thisstudy, we propose a single-round polymer-enhanced capillary transientisotachophoresis/gold nanoparticle (SR-PectI/AuNP) framework for developingsimple, highly sensitive DNA aptasensors. This framework comprisestwo key components: SR-PectI selection, which enables semicomprehensivescreening of family-level high-affinity aptamer candidates for bacterialtargets, and sensor development based on colorimetric changes in aptamer-conjugatedAuNPs. Notably, the entire SR-PectI/AuNP framework is completed withina short duration. In this proof-of-concept study, we targeted Legionella pneumophila (Lp); sevenhigh-affinity aptamers for Lp (1.0 ≤ Kd ≤ 14 nmol L–1; 9-foldlower than those of previously reported Lp aptamers)were successfully acquired via SR-PectI selection, followed by sensordevelopment using AuNPs. This enabled a detection limit of 1.1 ×102 cell equivalents for Lp, lower thanthat for commonly used immunosensors, within 40 min. The SR-PectI/AuNPframework for environmental bacterial species provides a comprehensiveapproach for developing simple analytical methods for threshold-basedscreening.","PeriodicalId":93847,"journal":{"name":"ACS ES&T water","volume":"6 9","pages":"5710–5722"},"PeriodicalIF":4.5,"publicationDate":"2026-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148950611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0