Electrochemical science advances最新文献

{"title":"Facile and Rapid Fabrication of a Glutamate Sensor via Electropolymerization of o-Aminophenol","authors":"Kyoko Sugiyama,&nbsp;Fumiya Sato,&nbsp;Yuto Kunitastu,&nbsp;Kota Honda,&nbsp;Yusuke Hirabayashi,&nbsp;Yasufumi Takahashi,&nbsp;Katsuhiko Sato","doi":"10.1002/elsa.70022","DOIUrl":"https://doi.org/10.1002/elsa.70022","url":null,"abstract":"<p>Microfluidic analysis enables precise fluid manipulation and high-time-resolution measurements in microscale environments, and has attracted considerable attention as an analytical platform suitable for detecting spatiotemporally fluctuating biomolecules such as neurotransmitters. Glutamate is a major excitatory neurotransmitter in the central nervous system, and its real-time quantification is essential for understanding neural function and pathological mechanisms. In this study, aiming for application to microfluidic analysis, glutamate oxidase was immobilised within an electropolymerized poly(o-aminophenol) film, allowing facile and highly reproducible fabrication of enzyme-modified electrodes. When the response characteristics were evaluated using platinum electrodes of 3 mm and 25 µm in diameter, the microelectrode exhibited a high current density derived from radial diffusion and yielded a steady-state response. The limit of detection and limit of quantification obtained using the 3 mm electrode were 3.22 and 10.7 µM, respectively. Furthermore, the fabricated electrode showed no measurable response toward coexisting substances such as glutamine, aspartic acid, GABA, and glucose, indicating high substrate specificity, and the maximum response was obtained at pH 7.4. These results demonstrate that enzyme immobilisation via electropolymerization is a practical method suitable for integration with microelectrodes and microfluidic devices, and is expected to contribute to in situ glutamate monitoring as well as future applications in clinical diagnostic systems.</p>","PeriodicalId":93746,"journal":{"name":"Electrochemical science advances","volume":"6 2","pages":""},"PeriodicalIF":4.1,"publicationDate":"2026-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://chemistry-europe.onlinelibrary.wiley.com/doi/epdf/10.1002/elsa.70022","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147686990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Facile and Rapid Fabrication of a Glutamate Sensor via Electropolymerization of o-Aminophenol","authors":"Kyoko Sugiyama,&nbsp;Fumiya Sato,&nbsp;Yuto Kunitastu,&nbsp;Kota Honda,&nbsp;Yusuke Hirabayashi,&nbsp;Yasufumi Takahashi,&nbsp;Katsuhiko Sato","doi":"10.1002/elsa.70022","DOIUrl":"https://doi.org/10.1002/elsa.70022","url":null,"abstract":"<p>Microfluidic analysis enables precise fluid manipulation and high-time-resolution measurements in microscale environments, and has attracted considerable attention as an analytical platform suitable for detecting spatiotemporally fluctuating biomolecules such as neurotransmitters. Glutamate is a major excitatory neurotransmitter in the central nervous system, and its real-time quantification is essential for understanding neural function and pathological mechanisms. In this study, aiming for application to microfluidic analysis, glutamate oxidase was immobilised within an electropolymerized poly(o-aminophenol) film, allowing facile and highly reproducible fabrication of enzyme-modified electrodes. When the response characteristics were evaluated using platinum electrodes of 3 mm and 25 µm in diameter, the microelectrode exhibited a high current density derived from radial diffusion and yielded a steady-state response. The limit of detection and limit of quantification obtained using the 3 mm electrode were 3.22 and 10.7 µM, respectively. Furthermore, the fabricated electrode showed no measurable response toward coexisting substances such as glutamine, aspartic acid, GABA, and glucose, indicating high substrate specificity, and the maximum response was obtained at pH 7.4. These results demonstrate that enzyme immobilisation via electropolymerization is a practical method suitable for integration with microelectrodes and microfluidic devices, and is expected to contribute to in situ glutamate monitoring as well as future applications in clinical diagnostic systems.</p>","PeriodicalId":93746,"journal":{"name":"Electrochemical science advances","volume":"6 2","pages":""},"PeriodicalIF":4.1,"publicationDate":"2026-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://chemistry-europe.onlinelibrary.wiley.com/doi/epdf/10.1002/elsa.70022","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147686989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electrosynthesized Fe-based, Cu-based and Fe-Cu Metal-Organic Framework Systems for Supercapacitor Applications","authors":"T. Campeol Marinho,&nbsp;P. Herrasti,&nbsp;A. Gómez-Avilés","doi":"10.1002/elsa.70021","DOIUrl":"https://doi.org/10.1002/elsa.70021","url":null,"abstract":"<p>A novel electrochemical method was successfully developed for the synthesis of monometallic MIL-100(Fe), HKUST-1 and Fe-Cu metal-organic framework (MOF) systems under mild conditions and short reaction times. X-ray diffraction confirmed the successful formation of the parent frameworks, showing strong agreement with simulated patterns from the literature. Structural analysis of the Fe-Cu MOF systems revealed the coexistence of Fe-based and Cu-based MOF phases, forming physical phase mixtures. Textural characterisation by N₂ adsorption-desorption measurements revealed high surface areas of 1,044 m²·g⁻¹ for MIL-100(Fe), 781 m²·g⁻¹ for HKUST-1 and up to 800 m²·g⁻¹ for selected Fe-Cu MOF systems. The electrochemical performance of MIL-100(Fe), HKUST-1 and Fe-Cu(3.75/15) system was evaluated using galvanostatic charge-discharge and cyclic voltammetry in a three-electrode configuration with conductive catalytic inks. All materials displayed hybrid supercapacitor behaviour, combining electric double-layer capacitance and pseudocapacitive contributions. The Fe-Cu MOF system (Fe-Cu(3.75/15)) demonstrated enhanced electrochemical performance, achieving a specific capacitance of 1,073 F·g⁻¹, an energy density of 205 Wh·kg⁻¹ at 1 A·g⁻¹, and a power density of 3,632 W·kg⁻¹ at 5 A·g⁻¹. The improved performance is attributed to the coexistence of Fe- and Cu-based MOF phases, which promote complementary redox activity and charge storage mechanisms. These results highlight the potential of electrochemically synthesised MOF phase mixtures as promising materials for high-performance supercapacitor applications.</p>","PeriodicalId":93746,"journal":{"name":"Electrochemical science advances","volume":"6 2","pages":""},"PeriodicalIF":4.1,"publicationDate":"2026-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://chemistry-europe.onlinelibrary.wiley.com/doi/epdf/10.1002/elsa.70021","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147686386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electrosynthesized Fe-based, Cu-based and Fe-Cu Metal-Organic Framework Systems for Supercapacitor Applications","authors":"T. Campeol Marinho,&nbsp;P. Herrasti,&nbsp;A. Gómez-Avilés","doi":"10.1002/elsa.70021","DOIUrl":"https://doi.org/10.1002/elsa.70021","url":null,"abstract":"<p>A novel electrochemical method was successfully developed for the synthesis of monometallic MIL-100(Fe), HKUST-1 and Fe-Cu metal-organic framework (MOF) systems under mild conditions and short reaction times. X-ray diffraction confirmed the successful formation of the parent frameworks, showing strong agreement with simulated patterns from the literature. Structural analysis of the Fe-Cu MOF systems revealed the coexistence of Fe-based and Cu-based MOF phases, forming physical phase mixtures. Textural characterisation by N₂ adsorption-desorption measurements revealed high surface areas of 1,044 m²·g⁻¹ for MIL-100(Fe), 781 m²·g⁻¹ for HKUST-1 and up to 800 m²·g⁻¹ for selected Fe-Cu MOF systems. The electrochemical performance of MIL-100(Fe), HKUST-1 and Fe-Cu(3.75/15) system was evaluated using galvanostatic charge-discharge and cyclic voltammetry in a three-electrode configuration with conductive catalytic inks. All materials displayed hybrid supercapacitor behaviour, combining electric double-layer capacitance and pseudocapacitive contributions. The Fe-Cu MOF system (Fe-Cu(3.75/15)) demonstrated enhanced electrochemical performance, achieving a specific capacitance of 1,073 F·g⁻¹, an energy density of 205 Wh·kg⁻¹ at 1 A·g⁻¹, and a power density of 3,632 W·kg⁻¹ at 5 A·g⁻¹. The improved performance is attributed to the coexistence of Fe- and Cu-based MOF phases, which promote complementary redox activity and charge storage mechanisms. These results highlight the potential of electrochemically synthesised MOF phase mixtures as promising materials for high-performance supercapacitor applications.</p>","PeriodicalId":93746,"journal":{"name":"Electrochemical science advances","volume":"6 2","pages":""},"PeriodicalIF":4.1,"publicationDate":"2026-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://chemistry-europe.onlinelibrary.wiley.com/doi/epdf/10.1002/elsa.70021","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147686293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stereolithographic Hydrogel Microfluidic Platform for Monitoring Cellular Respiration via Scanning Electrochemical Microscopy","authors":"Taiyo Kanno,&nbsp;Yoshinobu Utagawa,&nbsp;Yusuke Kanno,&nbsp;Fei Li,&nbsp;Hiroya Abe,&nbsp;Hitoshi Shiku,&nbsp;Kosuke Ino","doi":"10.1002/elsa.70020","DOIUrl":"10.1002/elsa.70020","url":null,"abstract":"<p>Hydrogel microfluidic devices have garnered significant interest as cell culture platforms owing to the efficient diffusion of nutrients through the hydrogel. Stereolithography, a versatile fabrication technique, has been widely adopted for the rapid prototyping of various microfluidic devices. Although evaluating cellular activity within microchannels during in vitro assays is essential, integrating electrochemical sensors into hydrogel devices remains challenging as these materials often cannot withstand conventional electrode fabrication processes. To address this limitation, scanning electrochemical microscopy was employed to assess cellular activity within hydrogel microfluidic devices, thereby eliminating the requirement for internal electrode fabrication. Initially, a novel hydrogel microfluidic design was developed, featuring accessible regions and thin hydrogel films. As a proof of concept, the respiratory activity of MCF-7 cells within a hydrogel microchannel was measured. This strategy is expected to facilitate future cell-based drug-screening applications.</p>","PeriodicalId":93746,"journal":{"name":"Electrochemical science advances","volume":"6 2","pages":""},"PeriodicalIF":4.1,"publicationDate":"2026-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://chemistry-europe.onlinelibrary.wiley.com/doi/epdf/10.1002/elsa.70020","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147562809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimizing Fire Suppression Strategies for Lithium-Ion Battery Thermal Runaway: A Comparative Study of Foam-Based Extinguishing Protocols","authors":"Zhongbin Fei,&nbsp;Jihua Zhang,&nbsp;Huanhuan Guo,&nbsp;Renjie Yang","doi":"10.1002/elsa.70019","DOIUrl":"https://doi.org/10.1002/elsa.70019","url":null,"abstract":"<p>The rapid adoption of electric vehicles (EVs) has intensified the focus on lithium-ion battery (LIB) fire safety, particularly the risks posed by thermal runaway (TR). This study evaluates the performance of a novel foam-based fire extinguishing agent under two distinct application protocols: Protocol 1, employing intermittent short-duration sprays, and Protocol 2, involving an initial prolonged spray followed by intermittent applications. Within the two tested configuration–protocol pairs, the intermittent-spray protocol achieved 2.75 times greater extinguishing efficiency than the prolonged-spray protocol, while conserving resources and providing sustained cooling. Soft-pack LIBs, with their layered structure, facilitated deeper foam penetration, resulting in faster cooling (2.8°C/s) and effective smoke suppression. In contrast, hard-shell LIBs, characterized by their rigid design, exhibited slower cooling (1.10°C/s) and prolonged smoke dissipation due to limited foam diffusion. These findings emphasize the importance of tailoring suppression strategies to battery design and highlight the superior performance of intermittent foam application. This work provides a framework for optimizing fire safety protocols in large LIB storage systems with freely accessible battery packs and offers configuration-specific insights rather than a full protocol ranking.</p>","PeriodicalId":93746,"journal":{"name":"Electrochemical science advances","volume":"6 1","pages":""},"PeriodicalIF":4.1,"publicationDate":"2026-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://chemistry-europe.onlinelibrary.wiley.com/doi/epdf/10.1002/elsa.70019","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146136121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High-Power Alkali-Free Direct Formate Fuel Cell Enabled by Optimized Ionomer Loading With a Cation-Exchange Membrane","authors":"Yiming Wang,&nbsp;Fahimah Abd Lah Halim,&nbsp;Madihah Miskan,&nbsp;Kakeru Fujiwara,&nbsp;Yugo Osaka,&nbsp;Akio Kodama,&nbsp;Takuya Tsujiguchi","doi":"10.1002/elsa.70017","DOIUrl":"https://doi.org/10.1002/elsa.70017","url":null,"abstract":"<p>Direct formate fuel cells (DFFCs) provide a safe liquid-fuel pathway for renewable energy storage, yet achieving high performance under alkali-free conditions remains challenging due to limitations in ion transport and catalyst-layer structure. Here, for the first time, a cation-exchange membrane (CEM) was combined with cationic ionomers (CI) in both catalyst layers to establish a fully alkali-free configuration, and the effects of ionomer loading were systematically examined. Optimizing the anode ionomer content to ionomer-to-carbon (I/C) ratio of 0.83 produced a well-balanced liquid–catalyst–ionomer triple-phase boundary and improved reaction kinetics. Fuel-composition analysis revealed that Na⁺ transport across the CEM accounted for only 20%–30% of the theoretical value, indicating that proton transport dominates charge compensation under alkali-free operation. At the cathode, reducing CI content enhanced oxygen transport by thinning the ionomer film and increasing access to catalytic sites, achieving a peak power density of 92 mW·cm⁻²—over twice that of previously reported alkali-free Na-ion-conducting DFFCs. Although lower ionomer loading increased HCOO⁻ crossover and accelerated voltage decay, these results demonstrate that appropriate CI tuning in both electrodes effectively balances oxygen transport, crossover and ion conduction, thereby enabling substantially improved performance in alkali-free DFFCs without external alkali additives.</p>","PeriodicalId":93746,"journal":{"name":"Electrochemical science advances","volume":"6 1","pages":""},"PeriodicalIF":4.1,"publicationDate":"2026-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://chemistry-europe.onlinelibrary.wiley.com/doi/epdf/10.1002/elsa.70017","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146135776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"K-MODEL: Kinetic Modelling and Electrochemical Mechanism of Dimethylamine Borane Oxidation","authors":"Huize Xue,&nbsp;Milad Torabfam,&nbsp;Jingfei Peng,&nbsp;Hamza Javed,&nbsp;Ella Joasil,&nbsp;Omowunmi Sadik","doi":"10.1002/elsa.70016","DOIUrl":"https://doi.org/10.1002/elsa.70016","url":null,"abstract":"&lt;p&gt;Here, we present K-MODEL (Kinetic Modelling framework for Electrochemical Mechanisms), a practical methodology that integrates electrochemical voltammetry, kinetic parameter extraction and simulation to unravel the electrochemical oxidation mechanism of dimethylamine borane (DMAB). DMAB is a key reducing agent used in hydrogen storage, pharmaceuticals, electroless plating and semiconductor fabrication, yet its reaction mechanism remains only partially understood. Determining kinetic and thermodynamic parameters is essential for understanding redox processes and optimizing electrochemical systems, but such data are often inconsistent or unavailable in literature. In this study, a combination of cyclic voltammetry (CV), chronoamperometry (CA) and hydrodynamic voltammetry (HDV), together with the self-developed open-source tool &lt;i&gt;Envismetrics&lt;/i&gt;, was used to determine essential parameters including the diffusion coefficient (&lt;span&gt;&lt;/span&gt;&lt;math&gt;\u0000 &lt;semantics&gt;\u0000 &lt;mi&gt;D&lt;/mi&gt;\u0000 &lt;annotation&gt;$D$&lt;/annotation&gt;\u0000 &lt;/semantics&gt;&lt;/math&gt;), standard rate constant (&lt;span&gt;&lt;/span&gt;&lt;math&gt;\u0000 &lt;semantics&gt;\u0000 &lt;msub&gt;\u0000 &lt;mi&gt;k&lt;/mi&gt;\u0000 &lt;mn&gt;0&lt;/mn&gt;\u0000 &lt;/msub&gt;\u0000 &lt;annotation&gt;${{k}_0}$&lt;/annotation&gt;\u0000 &lt;/semantics&gt;&lt;/math&gt;) and formal potential (&lt;span&gt;&lt;/span&gt;&lt;math&gt;\u0000 &lt;semantics&gt;\u0000 &lt;msub&gt;\u0000 &lt;mi&gt;E&lt;/mi&gt;\u0000 &lt;mi&gt;f&lt;/mi&gt;\u0000 &lt;/msub&gt;\u0000 &lt;annotation&gt;${{E}_f}$&lt;/annotation&gt;\u0000 &lt;/semantics&gt;&lt;/math&gt;). Simulations carried out using KISSA-1D software confirmed the experimental findings and identified a three-step continuous oxidation mechanism for DMAB. The formal potentials were determined as &lt;span&gt;&lt;/span&gt;&lt;math&gt;\u0000 &lt;semantics&gt;\u0000 &lt;mrow&gt;\u0000 &lt;mo&gt;−&lt;/mo&gt;\u0000 &lt;mn&gt;0.84&lt;/mn&gt;\u0000 &lt;mspace&gt;&lt;/mspace&gt;\u0000 &lt;mi&gt;V&lt;/mi&gt;\u0000 &lt;/mrow&gt;\u0000 &lt;annotation&gt;$ - 0.84;{mathrm{V}}$&lt;/annotation&gt;\u0000 &lt;/semantics&gt;&lt;/math&gt; (&lt;span&gt;&lt;/span&gt;&lt;math&gt;\u0000 &lt;semantics&gt;\u0000 &lt;msub&gt;\u0000 &lt;mi&gt;E&lt;/mi&gt;\u0000 &lt;mrow&gt;\u0000 &lt;mi&gt;f&lt;/mi&gt;\u0000 &lt;mn&gt;1&lt;/mn&gt;\u0000 &lt;/mrow&gt;\u0000 &lt;/msub&gt;\u0000 &lt;annotation&gt;${{E}_{{mathrm{f1}}}}$&lt;/annotation&gt;\u0000 &lt;/semantics&gt;&lt;/math&gt;), &lt;span&gt;&lt;/span&gt;&lt;math&gt;\u0000 &lt;semantics&gt;\u0000 &lt;mrow&gt;\u0000 &lt;mn&gt;0.12&lt;/mn&gt;\u0000 &lt;mspace&gt;&lt;/mspace&gt;\u0000 &lt;mi&gt;V&lt;/mi&gt;\u0000 &lt;/mrow&gt;\u0000 &lt;annotation&gt;$0.12;{mathrm{V}}$&lt;/annotation&gt;\u0000 &lt;/semantics&gt;&lt;/math&gt; (&lt;span&gt;&lt;/span&gt;&lt;math&gt;\u0000 &lt;semantics&gt;\u0000 &lt;msub&gt;\u0000 &lt;mi&gt;E&lt;/mi&gt;\u0000 &lt;mrow&gt;\u0000 &lt;","PeriodicalId":93746,"journal":{"name":"Electrochemical science advances","volume":"6 1","pages":""},"PeriodicalIF":4.1,"publicationDate":"2026-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://chemistry-europe.onlinelibrary.wiley.com/doi/epdf/10.1002/elsa.70016","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146136574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Perspective on the Technology Status of Chemical Hydrogen Carriers for Ship-Based Energy Import","authors":"Andreas Peschel,&nbsp;Peter Wasserscheid","doi":"10.1002/elsa.70015","DOIUrl":"https://doi.org/10.1002/elsa.70015","url":null,"abstract":"<p>The transport of hydrogen for the import of renewable energy will play an important role for the highly industrialized regions in Europe to meet their energy needs. As the storage and transportation of neat hydrogen comes with considerable downsides with respect to energy density and infrastructure compatibility, chemical hydrogen carriers are a promising concept to enable large-scale hydrogen import. This perspective discusses the advantages, limitations and current technology readiness levels of chemical hydrogen carriers for intercontinental transport by ship. In terms of rapid scale-up and market entry, ammonia and methanol are the most promising short-term options. If the market for chemical hydrogen carriers grows as expected, it is likely that chemical hydrogen carriers optimized for certain applications will coexist, optimizing the individual value chains.</p>","PeriodicalId":93746,"journal":{"name":"Electrochemical science advances","volume":"6 1","pages":""},"PeriodicalIF":4.1,"publicationDate":"2026-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://chemistry-europe.onlinelibrary.wiley.com/doi/epdf/10.1002/elsa.70015","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146136678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Urea Biosensor Based on a Field-Effect Capacitor Modified With a Stacked Weak Polyelectrolyte/Enzyme Bilayer","authors":"Astghik S. Tsokolakyan,&nbsp;Vardan A. Hayrapetyan,&nbsp;Derenik K. Petrosyan,&nbsp;Melanie Welden,&nbsp;Heiko Iken,&nbsp;Michael J. Schöning,&nbsp;Mkrtich A. Yeranosyan,&nbsp;Arshak Poghossian","doi":"10.1002/elsa.70013","DOIUrl":"https://doi.org/10.1002/elsa.70013","url":null,"abstract":"<p>Urea is formed from the metabolism of proteins and used as a biomarker for diagnosing and monitoring various medical conditions. In this work, a urea biosensor based on an electrolyte-insulator-semiconductor capacitor (EISCAP) modified with a stacked polyelectrolyte polyallylamine hydrochloride (PAH)/urease bilayer prepared by the layer-by-layer (LbL) technique is presented for the first time. The LbL formation of the PAH/urease bilayer was monitored with an underlying charge-sensitive Al/p-Si/SiO₂/Ta₂O₅ EISCAP using convenient capacitive-voltage and constant-capacitance mode measurements. Urea-sensitive EISCAP biosensors were electrochemically characterised in buffer solutions and artificial urine (AU) samples spiked with various concentrations of urea between 0.1 mM and 50 mM. The biosensors exhibited urea sensitivities of ca. 35.4 mV/dec and 32.1 mV/dec in buffer and AU solutions, respectively. Finally, local surface pH changes as a function of urea concentration have been evaluated. The obtained findings demonstrate the potential of PAH/urease-modified EISCAPs for non-invasive urea biomarker detection in urine samples at homecare or in-field settings.</p>","PeriodicalId":93746,"journal":{"name":"Electrochemical science advances","volume":"6 1","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://chemistry-europe.onlinelibrary.wiley.com/doi/epdf/10.1002/elsa.70013","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145964402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}