Bulletin of Entomological Research最新文献_第2页

Genome-wide identification of neuropeptides from Plutella xylostella (Lepidoptera: Plutellidae) and elucidating the essential roles of adipokinetic hormone in lipid mobilisation and food intake. 小菜蛾神经肽的全基因组鉴定及脂肪动力学激素在脂质动员和食物摄取中的重要作用。

IF 1.7 3区农林科学

Bulletin of Entomological Research Pub Date : 2026-09-15 DOI: 10.1017/S0007485326101254

Mei Fang, Jin-Hai Zhu, Jia-Ni Zhu, Jia-Xuan Xu, Si-Xian Gan, Zhen-Guo Yang, Qiu-Li Hou

{"title":"Genome-wide identification of neuropeptides from Plutella xylostella (Lepidoptera: Plutellidae) and elucidating the essential roles of adipokinetic hormone in lipid mobilisation and food intake.","authors":"Mei Fang, Jin-Hai Zhu, Jia-Ni Zhu, Jia-Xuan Xu, Si-Xian Gan, Zhen-Guo Yang, Qiu-Li Hou","doi":"10.1017/S0007485326101254","DOIUrl":"https://doi.org/10.1017/S0007485326101254","url":null,"abstract":"The diamondback moth, Plutella xylostella (L.) (Lepidoptera: Plutellidae), is a major pest of cruciferous crops and has developed high levels of resistance to many widely used insecticides. Neuropeptides are involved in regulating essential insect biological processes, which could be considered potential target genes for pest control. In this study, a total of 45 neuropeptide precursor genes were identified in the genome of P. xylostella, including two adipokinetic hormone genes (PxAKH1 and PxAKH2). The qPCR analysis showed that PxAKH genes were highly expressed in third- and fourth-instar larvae and exhibited high expression levels in the fat body, head, and gut of P. xylostella larvae. Additionally, short-term starvation stimulated food consumption in P. xylostella, which was consistent with the significant induction of PxAKH1 within 6 h. Subsequently, RNA interference (RNAi) was utilised to investigate the functions of PxAKH1 in P. xylostella, and the injection of dsPxAKH1 significantly suppressed target gene expression, achieving a maximum silencing efficiency of 73.03%. Notably, knockdown of PxAKH1 increased triacylglycerol levels from 23.67 ± 0.56 mg/g in the dsGFP control to 25.84 ± 0.64 mg/g and enhanced lipid storage, while markedly reducing food intake from 221.33 ± 9.10 mm2 to 123.33 ± 18.80 mm2 in P. xylostella larvae. In summary, these findings demonstrate that PxAKH1 plays a critical role in regulating lipid mobilisation and food intake in P. xylostella, indicating its potential as an RNAi-based target for pest control.","PeriodicalId":9370,"journal":{"name":"Bulletin of Entomological Research","volume":" ","pages":"1-14"},"PeriodicalIF":1.7,"publicationDate":"2026-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148966499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Direct and prey-mediated vitamin supplementation reshape predator-prey dynamics in Amblyseius swirskii (Acari: Phytoseiidae). 直接和猎物介导的维生素补充重塑了威氏钝绥螨（蜱螨目：植物绥螨科）的捕食者-猎物动态。

IF 1.7 3区农林科学

Bulletin of Entomological Research Pub Date : 2026-09-14 DOI: 10.1017/S000748532610131X

Marziye Jahanbazi, Arash Zibaee, Yaghoub Fathipour

{"title":"Direct and prey-mediated vitamin supplementation reshape predator-prey dynamics in Amblyseius swirskii (Acari: Phytoseiidae).","authors":"Marziye Jahanbazi, Arash Zibaee, Yaghoub Fathipour","doi":"10.1017/S000748532610131X","DOIUrl":"https://doi.org/10.1017/S000748532610131X","url":null,"abstract":"Micronutrient supplementation has been proposed as a strategy to improve factitious diets for predatory mites, yet the influence of nutrient delivery routes on predator performance remains poorly understood. We evaluated the demographic responses of Amblyseius swirskii (Athias-Henriot) to vitamins B complex, C, and E provided either directly through an artificial dry diet or indirectly via two astigmatid prey mites differing in nutritional suitability, Carpoglyphus lactis L. and Tyrophagus putrescentiae (Schrank). Direct supplementation through a vitamin-enriched dry diet resulted in severe developmental impairment and complete reproductive failure, with fewer than 10% of individuals reaching adulthood and no oviposition observed. In contrast, supplementation of the same diet with mixed pollen restored development and reproduction, supporting stable population performance over 20 generations. Prey-mediated vitamin delivery supported sustained demographic performance in both prey systems, including the nutritionally less suitable T. putrescentiae. Population growth parameters remained stable across generations, with intrinsic rates of increase (r) ranging from 0.129 to 0.142 day-1 on the pollen-supplemented dry diet, 0.140 to 0.152 day-1 on T. putrescentiae, and 0.176 to 0.185 day-1 on C. lactis. No evidence of cumulative negative generational effects was detected under indirect supplementation. These findings indicate that predator responses to micronutrient supplementation depend strongly on the mode of nutrient delivery and suggest that trophic mediation may play an important role in stabilising nutrient utilisation in predatory mite rearing systems. The study provides new insight by demonstrating that micronutrient delivery route, rather than vitamin supplementation alone, is a key determinant of predator demographic performance.","PeriodicalId":9370,"journal":{"name":"Bulletin of Entomological Research","volume":" ","pages":"1-13"},"PeriodicalIF":1.7,"publicationDate":"2026-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148963118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Temporal regulation of miRNA 92a and miRNA 279d on PaEno1 expression and dynamic responses of metal-stress-related genes to cadmium exposure in Propsilocerus akamusi (Diptera: Chironomidae) larvae. microrna 92a和microrna 279d对赤色拟盲蝽幼虫PaEno1表达的时间调控及金属胁迫相关基因对镉暴露的动态响应

IF 1.7 3区农林科学

Bulletin of Entomological Research Pub Date : 2026-09-14 DOI: 10.1017/S000748532610128X

Jing Xue, Yimei Chen, Ping Li, Yuexia Zhang, Xianyun Zheng

{"title":"Temporal regulation of miRNA 92a and miRNA 279d on PaEno1 expression and dynamic responses of metal-stress-related genes to cadmium exposure in Propsilocerus akamusi (Diptera: Chironomidae) larvae.","authors":"Jing Xue, Yimei Chen, Ping Li, Yuexia Zhang, Xianyun Zheng","doi":"10.1017/S000748532610128X","DOIUrl":"https://doi.org/10.1017/S000748532610128X","url":null,"abstract":"Previous studies have indicated that Eno1 may play an important role in Cadmium (Cd) resistance. However, the potential regulatory mechanisms of Eno1 in response to Cd2+ stress are still limited. In this study, we hypothesised that Cd-induced miRNA alteration could effectively regulate PaEno1 (Eno1 in Propsilocerus akamusi (Diptera: Chironomidae)) under Cd2+ stress. By integrating Illumina miRNA sequencing, real-time quantitative Polymerase chain reaction (PCR) (RT-qPCR) validation, PaEno1 target prediction and in vivo functional assays, we verified that PaEno1 is a putative target of miR-92a and miR-279d, which potentially mediate the post-transcriptional regulation of PaEno1 under Cd2⁺ stress. RT-qPCR revealed significant downregulation of miR-92a and miR-279d by 2.825-4.225-fold upon long-term Cd exposure. Agomir/antagomir-mediated overexpression and knockdown of the two miRNAs exerted no significant impact on larval survival (Log-rank test, p > 0.05). The hazard ratios were 0.715 and 0.609 for miR-279d (agomir and antagomir), and 0.810 and 0.869 for miR-92a, respectively. The 95% confidence intervals spanned 0.266-1.921 and 0.217-1.712 for miR-279d agomir and antagomir, and 0.399-1.642 and 0.434-1.740 for miR-92a agomir and antagomir, with all intervals crossing the null value of 1. Agomir-induced upregulation of miR-92a and miR-279d dynamically altered the expression of PaEno1, HbV, HbVII, alpha-esterase, and carboxylesterase. Overall, miR-92a and miR-279d act as temporal post-transcriptional regulators of PaEno1 rather than key modulators of Cd tolerance. This work underscores the complexity of miRNA-regulated networks in P. akamusi responding to Cd stress.","PeriodicalId":9370,"journal":{"name":"Bulletin of Entomological Research","volume":" ","pages":"1-12"},"PeriodicalIF":1.7,"publicationDate":"2026-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148963134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular characterization of vitellogenin and its receptor with CRISPR-based sgRNA validation in the legume pod borer, Maruca vitrata (Geyer) (Lepidoptera: Crambidae). 利用基于crispr的sgRNA验证豆科荚螟（Maruca vitrata Geyer）卵黄蛋白原及其受体的分子特征。

IF 1.7 3区农林科学

Bulletin of Entomological Research Pub Date : 2026-09-10 DOI: 10.1017/S0007485326101308

Chalapathi Pradeep, Parvathy Madhusoodanan Sujatha, Somakalahalli Narasimhappa Nagesha, Ramasamy Asokan, Ashok Karuppannasamy

{"title":"Molecular characterization of vitellogenin and its receptor with CRISPR-based sgRNA validation in the legume pod borer, Maruca vitrata (Geyer) (Lepidoptera: Crambidae).","authors":"Chalapathi Pradeep, Parvathy Madhusoodanan Sujatha, Somakalahalli Narasimhappa Nagesha, Ramasamy Asokan, Ashok Karuppannasamy","doi":"10.1017/S0007485326101308","DOIUrl":"https://doi.org/10.1017/S0007485326101308","url":null,"abstract":"Maruca vitrata, the legume pod borer, causes yield losses of up to 80% in grain legumes. Increasing insecticide resistance and environmental concerns necessitate sustainable pest management alternatives. In the present study, the complete vitellogenin (Vg) coding sequence (CDS), a key reproductive gene involved in oogenesis and embryonic development, was cloned and molecularly characterised from M. vitrata. The assembled Vg CDS (∼5.3 kb) shared 99.04% sequence identity with the reported M. vitrata Vg sequence (MG799570.1). Phylogenetic analysis demonstrated close evolutionary association with related Lepidopteran species, while protein domain analysis identified three conserved domains, namely LPD_N, DUF1943, and VWD. Among these, the single exon-encoded LPD_N domain was selected as the target region for CRISPR/Cas9-mediated editing. Homology models of Vg and vitellogenin receptor (VgR) (Global Model Quality Estimation (GMQE): 0.58 and 0.51) showed a favourable interaction by protein-protein docking (score: -295.66). Three single-guide RNAs (sgRNAs) were designed, synthesised through in-vitro transcription, and evaluated using in vitro cleavage assays. sgRNA1 targeting the LPD_N domain and sgRNA2 targeting the signal peptide region exhibited efficient site-specific cleavage activity, whereas sgRNA3 failed to induce cleavage because of an unfavourable secondary structure that likely impaired Cas9-sgRNA complex formation. Overall, this study provides the first CRISPR-oriented functional characterisation and sgRNA validation of the M. vitrata Vg gene, together with structural characterisation of VgR and Vg-VgR interaction analysis, providing preliminary molecular resources for future CRISPR/Cas9 studies and supporting future embryo microinjection and heritable genome editing for sustainable management of M. vitrata.","PeriodicalId":9370,"journal":{"name":"Bulletin of Entomological Research","volume":" ","pages":"1-13"},"PeriodicalIF":1.7,"publicationDate":"2026-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148918367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Parasitic capacity of Chelonus bifoveolatus Szépligeti (Hymenoptera: Braconidae) on fall armyworm reared on maize, millet, and sorghum. 双斑切茧蜂（膜翅目：茧蜂科）对玉米、谷子和高粱上饲养的秋粘虫的寄生能力。

IF 1.7 3区农林科学

Bulletin of Entomological Research Pub Date : 2026-08-10 DOI: 10.1017/S0007485326101266

Simsin Fatoumata Ganou, Florence Sankara, Aïchatou Nadia Christelle Dao, Fernand Sankara, Marc Kenis

{"title":"Parasitic capacity of Chelonus bifoveolatus Szépligeti (Hymenoptera: Braconidae) on fall armyworm reared on maize, millet, and sorghum.","authors":"Simsin Fatoumata Ganou, Florence Sankara, Aïchatou Nadia Christelle Dao, Fernand Sankara, Marc Kenis","doi":"10.1017/S0007485326101266","DOIUrl":"https://doi.org/10.1017/S0007485326101266","url":null,"abstract":"Several species of parasitoids associated with the fall armyworm, Spodoptera frugiperda, have been identified in Burkina Faso, among which Chelonus bifoveolatus, an egg-larval parasitoid, is the most abundant. This study was conducted in the region of Bobo-Dioulasso, Western Burkina Faso, from April 2023 to February 2024 under ambient laboratory conditions. The objective was to investigate the biological parameters of C. bifoveolatus reared on S. frugiperda on different host plants and at different periods of the year. Egg masses laid on maize, millet, and sorghum leaves were exposed daily to female parasitoids. The parameters studied included parasitism rate, cocoon formation rate, adult emergence rate, post-embryonic and cocoon development duration, sex-ratio, and lifespan. Parasitism rates varied between 48% and 80%, the pupation rate between 48.00% and 99.43% and the emergence rate between 27.60% and 84.91%. Post-embryonic development lasted between 14.18 ± 2.05 and 28.52 ± 2.87 days, while cocoon development ranged from 9.23 ± 1.18 to 21.29 ± 1.63 days. The sex ratio favoured males, with at least two males for every female. The adult lifespan ranged from 10.22 ± 3.74 to 13.34 ± 4.60 days. In general, performances did not vary much with host plants but were better during the rainy season than during the dry season. This study provides important information about C. bifoveolatus with a view to its use as a biological control agent of the fall armyworm.","PeriodicalId":9370,"journal":{"name":"Bulletin of Entomological Research","volume":" ","pages":"1-9"},"PeriodicalIF":1.7,"publicationDate":"2026-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148700612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular cloning and expression under cold stress of two heat shock proteins from Dastarcus helophoroides (Fairmaire) (Coleoptera: Bothrideridae). 两种热休克蛋白的克隆及其在冷胁迫下的表达（鞘翅目：双蓟马科）。

IF 1.7 3区农林科学

Bulletin of Entomological Research Pub Date : 2026-07-30 DOI: 10.1017/S0007485326101229

Yanchen Wang, Defu Chi

{"title":"Molecular cloning and expression under cold stress of two heat shock proteins from Dastarcus helophoroides (Fairmaire) (Coleoptera: Bothrideridae).","authors":"Yanchen Wang, Defu Chi","doi":"10.1017/S0007485326101229","DOIUrl":"https://doi.org/10.1017/S0007485326101229","url":null,"abstract":"Heat shock proteins (HSPs) maintain cellular homeostasis and regulate immune responses across species. HSP90 stabilises and activates regulatory proteins, while HSP70 facilitates protein folding and prevents aggregation. In this study, the complete cDNA sequences of Dastarcus helophoroides - HSP70, HSP90, and β-actin (used as a reference for real-time quantitative polymerase chain reaction) - were obtained using rapid amplification of cDNA ends-polymerase chain reaction, and the expression of HSP70 and HSP90 under cold stress was analysed. The full-length cDNA of HSP90 contains a 2346-bp open reading frame (ORF) encoding 781 amino acids with a molecular weight (MW) of 89.6 kDa. The HSP70 cDNA contains a 1911-bp ORF encoding 636 amino acids (MW: 69.7 kDa), and the β-actin cDNA contains a 1131-bp ORF encoding 376 amino acids (MW: 41.7 kDa). Cold stress significantly affected HSP expression: HSP90 expression peaked at -15 °C, with a 9.45-fold increase compared to the control (P < 0.05), whereas HSP70 expression increased markedly at -10 °C, with a 60.42-fold increase compared to the control (P < 0.05). These findings have important biological implications for predicting insect performance under fluctuating thermal environments and for optimising low-temperature storage and release strategies of D. helophoroides in biological control programmes.","PeriodicalId":9370,"journal":{"name":"Bulletin of Entomological Research","volume":" ","pages":"1-10"},"PeriodicalIF":1.7,"publicationDate":"2026-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148618048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bionomics, morphometrics, and seasonal incidence of Eublemma amabilis Moore (Lepidoptera: Noctuidae) infesting lac insect under Assam conditions. 在阿萨姆邦条件下，侵染紫虱的绿脓杆菌（鳞翅目：夜蛾科）的生物学、形态计量学和季节性发病率。

IF 1.7 3区农林科学

Bulletin of Entomological Research Pub Date : 2026-07-27 DOI: 10.1017/S0007485326101205

Binita Borah, Purnima Das, Ushasri Banna, Surajit Kalita, Priyanka Saikia, Vaibhav D Lohot, Mohansundaram Arumugam

{"title":"Bionomics, morphometrics, and seasonal incidence of Eublemma amabilis Moore (Lepidoptera: Noctuidae) infesting lac insect under Assam conditions.","authors":"Binita Borah, Purnima Das, Ushasri Banna, Surajit Kalita, Priyanka Saikia, Vaibhav D Lohot, Mohansundaram Arumugam","doi":"10.1017/S0007485326101205","DOIUrl":"https://doi.org/10.1017/S0007485326101205","url":null,"abstract":"This study examined the bio-ecology and seasonal incidence of Eublemma amabilis infesting Kerria lacca and K. chinensis during 2019-2021 at Assam Agricultural University, Jorhat. The pre-oviposition period of E. amabilis was shorter in summer (4.74 ± 1.42 days) than in winter (6.80 ± 1.22 days). Fecundity was higher in summer, with females laying 70.4 ± 8.52 eggs compared to 38.4 ± 8.52 eggs in winter, with hatching success of 83.66 ± 10.32% and 67.81 ± 7.62%, respectively. Incubation periods were 5.90 ± 0.70 days (summer) and 8.80 ± 0.45 days (winter). Larval development lasted 24.80 ± 3.56 days in summer and 32.40 ± 4.39 days in winter. Pupation required 6-8 days in summer and 8-11 days in winter. The hypognathous head bore green compound eyes. Antennae measured 2.89 ± 0.11 mm with 36-40 segments. Forewings measured 5.14 ± 2.30 mm (male) and 7.22 ± 0.57 mm (female), while hindwings measured 4.45 ± 0.09 mm (male) and 6.94 ± 0.15 mm (female). Peak predator activity occurred during July-August (summer) and January-February (winter). Population incidence showed a positive association with temperature and relative humidity, with an increase in larval abundance under warm and humid conditions. These findings provide baseline information and could contribute to the development of weather-based forecasting models to support timely management of E. amabilis and strengthen lac insect conservation.","PeriodicalId":9370,"journal":{"name":"Bulletin of Entomological Research","volume":" ","pages":"1-12"},"PeriodicalIF":1.7,"publicationDate":"2026-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148590537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Interspecific variation in the fruit infestation level by Anastrepha fraterculus and Ceratitis capitata in northwestern Argentina mirrors the types of land use and host plant origin. 在阿根廷西北部地区，长尾角霉和头角霉侵染水平的种间变异反映了土地利用类型和寄主植物来源。

IF 1.7 3区农林科学

Bulletin of Entomological Research Pub Date : 2026-06-25 DOI: 10.1017/S0007485326101199

Analía Benavidez, Pablo Schliserman, Mariano Ordano

{"title":"Interspecific variation in the fruit infestation level by Anastrepha fraterculus and Ceratitis capitata in northwestern Argentina mirrors the types of land use and host plant origin.","authors":"Analía Benavidez, Pablo Schliserman, Mariano Ordano","doi":"10.1017/S0007485326101199","DOIUrl":"https://doi.org/10.1017/S0007485326101199","url":null,"abstract":"Invasive fruit flies have the potential to exert a profound influence on agroecosystems, given their capacity to displace native species and modify host-use dynamics. In northwestern Argentina, Anastrepha fraterculus and Ceratitis capitata are the most significant fruit fly pests. This study synthesised infestation data by A. fraterculus and C. capitata on host plant species in northwestern Argentina, and explored how land use and altitude modulate infestation patterns. The findings demonstrated that A. fraterculus exhibited significantly higher and more variable infestation levels, particularly on native hosts belonging to the families Myrtaceae and Juglandaceae. Infestation levels exhibited significant variation among the landscape units, increasing with altitude. Conversely, C. capitata exhibited lower and more homogeneous infestation levels, with no substantial disparities related to host plant origin or landscape unit, although a notable non-linear relationship with elevation was observed. The findings indicate ecological niche partitioning between the two species and highlight the necessity of integrating host use, environmental gradients, and landscape context into fruit fly management. By integrating biotic and abiotic drivers of infestation across multiple environmental contexts, our systematic study provides a novel, landscape-scale perspective on the ecology of both native and invasive fruit flies, suggesting practical implications for improving pest surveillance and control strategies through habitat-based approaches tailored to species-specific ecology.","PeriodicalId":9370,"journal":{"name":"Bulletin of Entomological Research","volume":" ","pages":"1-8"},"PeriodicalIF":1.7,"publicationDate":"2026-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148315700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Modulating effect of plant growth-promoting rhizobacteria on wheat-induced resistance to Schizaphis graminum. 植物促生根瘤菌对小麦抗裂蚜的调控作用。

IF 1.7 3区农林科学

Bulletin of Entomological Research Pub Date : 2026-06-22 DOI: 10.1017/S0007485326101072

Sabura Mirzamohamadi, Mojtaba Hosseini

{"title":"Modulating effect of plant growth-promoting rhizobacteria on wheat-induced resistance to Schizaphis graminum.","authors":"Sabura Mirzamohamadi, Mojtaba Hosseini","doi":"10.1017/S0007485326101072","DOIUrl":"https://doi.org/10.1017/S0007485326101072","url":null,"abstract":"The green bug aphid, Schizaphis graminum, is one of the most serious pests of wheat which cause considerable yield loss in wheat fields. At present, beneficial microorganisms such as plant growth-promoting rhizobacteria (PGPR) need to be incorporated into wheat production to diminish the high risk of heavily insecticide application for environment and health hazards. This study aimed to assess the influence of several PGPR strains, Pseudomonas geniculata, Azospirillum oryzae, Pseudomonas brassicacearum, and Azotobacter chroococcum, applied as seed treatments of wheat, on the life table parameters and foraging behaviour of the green aphid, S. graminum. We investigated the physiological and biochemical responses of wheat plants under aphid stress to elucidate the induced resistance conferred by PGPRs. Preference tests revealed that plants treated with A. chroococcum were the least favoured by aphids at 24 h post-infestation. Furthermore, biological parameters of the aphids exhibited significant variation across treatments, with the highest values recorded in the control group and the lowest in plants treated with A. chroococcum. The intrinsic rate of increase (rm) of the aphids was diminished following the application of PGPR, with the lowest rm observed in the A. chroococcum treatment. The highest concentrations of proteins, carbohydrates, total phenols, and antioxidant enzymes were detected in infested plants treated with A. chroococcum 72 h post-infestation. According to our results, A. chroococcum emerged as the most effective treatment for enhancing both antixenosis and antibiosis resistance in wheat against S. graminum, suggesting its significant potential for integration into pest management strategies in wheat cultivation.","PeriodicalId":9370,"journal":{"name":"Bulletin of Entomological Research","volume":" ","pages":"1-12"},"PeriodicalIF":1.7,"publicationDate":"2026-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148293492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular monitoring of insecticide resistance in Aphis gossypii Glover (Hemiptera: Aphididae) from different crops in Greece, using novel ddPCR diagnostics. 希腊不同作物棉蚜（半翅目：蚜科）对杀虫剂抗性的分子监测。

IF 1.7 3区农林科学

Bulletin of Entomological Research Pub Date : 2026-06-19 DOI: 10.1017/S0007485326101175

John Margaritopoulos, Konstantinos Mavridis, Aris Ilias, Kyriaki Maria Papapostolou, Anthi Tsingene, Panagiotis Skouras, Nikolaos Papadopoulos, John Vontas

{"title":"Molecular monitoring of insecticide resistance in Aphis gossypii Glover (Hemiptera: Aphididae) from different crops in Greece, using novel ddPCR diagnostics.","authors":"John Margaritopoulos, Konstantinos Mavridis, Aris Ilias, Kyriaki Maria Papapostolou, Anthi Tsingene, Panagiotis Skouras, Nikolaos Papadopoulos, John Vontas","doi":"10.1017/S0007485326101175","DOIUrl":"https://doi.org/10.1017/S0007485326101175","url":null,"abstract":"Aphis gossypii Glover is a highly polyphagous pest causing significant economic losses worldwide. In Greece, while the species is a primary threat to various crops, comprehensive molecular data regarding its insecticide resistance status remain scarce. This study, the first time in Greece, developed and implemented droplet digital PCR (ddPCR) to detect and quantify the frequency of key resistance mutations (R81T, S431F, A302S, L1014F/M918L, and A2666V - resistance to nicotinic acetylcholine receptor competitive modulators, dimethyl carbamates, organophosphates and carbamates, pyrethroids, and keto-enols, respectively) in field populations from citrus, cotton, and cucurbits plantations between 2021 and 2025. Our results identified four out of the six target mutations (L1014F and A2666V mutations were not found in any of the samples). Cucurbit populations exhibited the highest resistance levels, with S431F being fixed in seven populations and reaching a total resistance allele frequency (RAF) of 75%. The mutations A302S and M918L were also prevalent in cucurbits (total RAF of 40% and 49%, respectively), while the neonicotinoid-associated R81T mutation was detected at lower frequencies (total RAF of 9%). In contrast, citrus and cotton populations showed lower resistance diversity, with citrus populations possessing only the S431F mutation. The high resistance frequencies in cucurbits likely reflect intense selection pressure from specific insecticides used in these crops. Given the predominantly asexual reproduction of A. gossypii in Greece, the persistence of resistant genotypes poses a significant challenge. The ddPCR diagnostics developed here provide highly sensitive tools for monitoring target-site resistance, supporting effective insecticide resistance management.","PeriodicalId":9370,"journal":{"name":"Bulletin of Entomological Research","volume":" ","pages":"1-6"},"PeriodicalIF":1.7,"publicationDate":"2026-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148276009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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