Bulletin of Entomological Research最新文献

{"title":"Evidence of circulating recombinants between deformed wing virus and <i>Varroa destructor</i> virus-1 in honey bee colonies in Türkiye.","authors":"Murat Şevik, Aykut Zerek, İpek Erdem, Mehmet Yaman","doi":"10.1017/S000748532400052X","DOIUrl":"https://doi.org/10.1017/S000748532400052X","url":null,"abstract":"<p><p>Deformed wing virus (DWV), which is an important honey bee virus transmitted by <i>Varroa destructor (V. destructor)</i>, causes colony losses in honey bee colonies. This study aimed to investigate the prevalence and genetic diversity of DWV in honey bees in Türkiye and to determine the role of <i>V. destructor</i> in the transmission of the genetic variants of DWV. Honey bee samples were collected from 62 apiaries, by simple random sampling, during March 2022 and April 2023. The presence of <i>V. destructor</i> in collected bee samples was examined using a stereo microscope. Real-time RT-PCR was used for the detection of DWV-A and DWV-B (<i>Varroa destructor</i> virus-1 (VDV-1)) viruses. Genetic characterisation of the positive samples was conducted by sequencing polyprotein genomic region. Considering the <i>V. destructor</i> infestation rate of 3% as relevant, out of the 62 apiaries examined, 17 (27.4%) were positive. However, DWV-A and VDV-1 specific RNA was not detected in <i>V. destructor</i> samples. VDV-1 specific RNA was detected in 6.5% (4/62) of the apiaries, whereas DWV-A was not detected in the sampled apiaries. Phylogenetic analysis showed that isolates detected in this study were located in a separate cluster from previously characterised DWV-A and VDV-1 isolates. According to RDP4 and GARD analyses, DWV-VDV-1 recombination breakpoints were detected in field isolates. To the best our knowledge, this is the first report of the presence of VDV-1-DWV recombinants in Türkiye. Further studies are needed to determine the impact of VDV-1-DWV recombinants and their virological and antigenic properties.</p>","PeriodicalId":9370,"journal":{"name":"Bulletin of Entomological Research","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142495573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptomic analysis of the gonads of <i>Locusta migratoria</i> (Orthoptera: Acrididae) following infection with <i>Paranosema locustae</i>.","authors":"Xuewei Kong, Xinrui Guo, Jun Lin, Hui Liu, Huihui Zhang, Hongxia Hu, Wangpeng Shi, Rong Ji, Roman Jashenko, Han Wang","doi":"10.1017/S0007485324000592","DOIUrl":"https://doi.org/10.1017/S0007485324000592","url":null,"abstract":"<p><p><i>Paranosema locustae</i> is an environmentally friendly parasitic predator with promising applications in locust control. In this study, transcriptome sequencing was conducted on gonadal tissues of <i>Locusta migratoria</i> males and females infected and uninfected with <i>P. locustae</i> at different developmental stages. A total of 18,635 differentially expressed genes (DEGs) were identified in female ovary tissue transcriptomes, with the highest number of DEGs observed at 1 day post-eclosion (7141). In male testis tissue transcriptomes, a total of 32,954 DEGs were identified, with the highest number observed at 9 days post-eclosion (11,245). Venn analysis revealed 25 common DEGs among female groups and 205 common DEGs among male groups. Gene ontology and Kyoto Encyclopaedia of Genes and Genome analyses indicated that DEGs were mainly enriched in basic metabolism such as amino acid metabolism, carbohydrate metabolism, lipid metabolism, and immune response processes. Protein-protein interaction analysis results indicated that <i>L. migratoria</i> regulates the expression of immune- and reproductive-related genes to meet the body's demands in different developmental stages after <i>P. locustae</i> infection. Immune- and reproductive-related genes in <i>L. migratoria</i> gonadal tissue were screened based on database annotation information and relevant literature. Genes such as <i>Tsf</i>, <i>Hex1</i>, <i>Apolp-III</i>, <i>Serpin</i>, <i>Defense</i>, <i>Hsp70</i>, <i>Hsp90</i>, <i>JHBP</i>, <i>JHE</i>, <i>JHEH1</i>, <i>JHAMT</i>, and <i>VgR</i> play important roles in the balance between immune response and reproduction in gonadal tissues. For transcriptome validation, <i>Tsf</i>, <i>Hex1</i>, and <i>ApoLp-III</i> were selected and verified by quantitative real-time polymerase chain reaction (qRT-PCR). Correlation analysis revealed that the qRT-PCR expression patterns were consistent with the RNA-Seq results. These findings contribute to further understanding the interaction mechanisms between locusts and <i>P. locustae</i>.</p>","PeriodicalId":9370,"journal":{"name":"Bulletin of Entomological Research","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142495575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Making sense of chromosome polymorphisms in two leptysmine grasshoppers.","authors":"Pablo C Colombo","doi":"10.1017/S0007485324000579","DOIUrl":"https://doi.org/10.1017/S0007485324000579","url":null,"abstract":"<p><p>The touchstone of the 'New Synthesis' was population cytogenetics -rather than genetics - due to the abundant polymorphic inversions in the genus Drosophila. Grasshoppers were not a material of choice because of their conservative karyotypes. However, nowadays seven species of Acrididae were described for polymorphic centric fusions, five of them in South-America. <i>Leptysma argentina</i> and the likely biocontrol of water-hyacinth <i>Cornops aquaticum</i> are semiaquatic Leptysminae (Acrididae: Orthoptera), polymorphic for centric fusions, supernumerary segments and a B-chromosome. We sought to demonstrate the operation of natural selection on them, by detecting: (I) latitudinal clines; (II) regression on environmental variables; (III) deviation from null models, such as linkage equilibrium; (IV) seasonal variation; (V) comparison between age classes and (VI) selection component analyses. All of them were confirmed in <i>L. argentina</i>, just (I) and (II) in <i>C</i>. <i>aquaticum.</i> Furthermore, the relationship between karyotype, phenotype and recombination was confirmed in both species. Karyotype-phenotype relationship may be due to the body enlargement the fusions are associated with, along with a latitudinal transition in voltinism. Karyotype-related recombination reduction in both species may help explain all fusion clines, although there is probably more than one factor at work. No effects were noticed for a supernumerary segment in <i>L. argentina</i>, but it is ubiquitous and certainly non-neutral. <i>C. aquaticum</i> is poised for introduction in South-Africa as a biocontrol of water-hyacinths; the recent discovery of four more segment polymorphisms may imply more chromosomal markers to make sense of its genetic system.</p>","PeriodicalId":9370,"journal":{"name":"Bulletin of Entomological Research","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142495574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reproductive response of the predator <i>Tenuisvalvae notata</i> (Mulsant) (Coleoptera: Coccinellidae) to temperatures outside their ideal thermal range.","authors":"Enggel Beatriz S Carmo, Christian S A Silva-Torres, Jorge Braz Torres","doi":"10.1017/S0007485324000476","DOIUrl":"https://doi.org/10.1017/S0007485324000476","url":null,"abstract":"<p><p>Global warming has driven changes in the biology and fitness of organisms that need to adapt to temperatures outside of their optimal range to survive. This study investigated aspects of reproduction and survival of the lady beetle <i>Tenuisvalvae notata</i> (Mulsant) (Coleoptera: Coccinellidae) subjected to temperatures that varied from its optimal (28°C) to a gradual decrease (12, 14, 16, and 18°C) and increase (32, 34, 35, and 36°C) over time at a rate of 1°C/day. Fertility, fecundity, oviposition period, and survival were determined. There was a significant reduction in fertility and fecundity at temperatures below 18°C and above 34°C, whereas survival was reduced only above 34°C. Additionally, we evaluated that fecundity was the lowest when females were kept at low temperature, and when males were kept under high temperature. Therefore, if the <i>T. notata</i> remained for a long period under exposure to temperatures outside the ideal range, then the species could present different reproductive responses for each sex to high and low temperatures. This factor must be considered when releasing natural enemies into an area to understand the effect of temperature on the decline of a local population a few generations after release.</p>","PeriodicalId":9370,"journal":{"name":"Bulletin of Entomological Research","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142458592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling symbiotic bacterial communities in insects feeding on the latex-rich plant <i>Ficus microcarpa</i>.","authors":"Waleed Afzal Naveed, Qian Liu, Congcong Lu, Xiaolei Huang","doi":"10.1017/S0007485324000439","DOIUrl":"https://doi.org/10.1017/S0007485324000439","url":null,"abstract":"<p><p>The diversity and health of insects that feed on plants are closely related to their mutualistic symbionts and host plants. These symbiotic partners significantly influence various metabolic activities in these insects. However, the symbiotic bacterial community of toxic plant feeders still needs further characterisation. This study aims to unravel bacterial communities associated with the different species of insect representing three insect orders: Thysanoptera, Hemiptera, and Lepidoptera, along with their predicted functional role, which exclusively feeds on latex-rich plant species <i>Ficus microcarpa</i>. By using 16S rRNA gene high-throughput sequencing, the analysis was able to define the major alignment of the bacterial population, primarily comprising <i>Proteobacteria</i>, <i>Firmicutes</i>, <i>Bacteroidota</i>, <i>Actinobacteriota</i>, and <i>Acidobacteriota</i>. Significant differences in symbiotic organisms between three insect groups were discovered by the study: hemipterans had <i>Burkholderia</i> and <i>Buchnera</i>, and lepidopterans had <i>Acinetobacter</i>. At the same time, <i>Pseudomonas</i> was detected in high abundance in both lepidopteran and thysanopteran insects. Furthermore, these symbionts exhibit consistent core functions, potentially explaining how different insects can consume the same host plant. The identified core functions of symbionts open avenues for innovative approaches in utilising these relationships to develop environment-friendly solutions for pest control, with broader implications for agriculture and environmental conservation.</p>","PeriodicalId":9370,"journal":{"name":"Bulletin of Entomological Research","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142458593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lethal and sublethal concentrations spirodiclofen stress may increase the adaptation of <i>Panonychus citri</i> (Acari: Tetranychidae).","authors":"Hongyan Wang, Haifeng Wang, Kexin Wen, Tao Xie, Shigan Luo, Jiawei Wu, Bin Xia","doi":"10.1017/S0007485324000087","DOIUrl":"https://doi.org/10.1017/S0007485324000087","url":null,"abstract":"<p><p><i>Panonychus citri</i> is one of the most destructive pests in citrus orchards, exhibiting varying degrees of tolerance to numerous insecticides, such as spirodiclofen. To effectively manage pests, this study explores the response of <i>P. citri</i> to spirodiclofen stress from the perspectives of life history, enzymatic parameters, and reproduction. The effects of two concentrations (LC₃₀ and LC₅₀) of spirodiclofen on the biological parameters of <i>P. citri</i> were evaluated by the life table method. The results showed that the development duration, fecundity, oviposition days, and lifespan were shortened, though the pre-oviposition period of two treatments was prolonged in comparison with the control. A significant decrease was recorded in the net reproductive rate (<i>R</i>₀) and the mean generation time (<i>T</i>) for the two treatments. Nevertheless, the intrinsic rate of increase (<i>r</i>) and the rate of increase (<i>λ</i>) were not significantly affected in the LC₃₀ treatment, whereas they declined in the LC₅₀ treatment. The enzyme activity assay resulted in higher activities of catalase (CAT), superoxide dismutase (SOD), peroxidase (POD), and carboxylesterase (CarE), among the treatments than the control. In contrast, the treatments recorded lower cytochromeP450 (CYP450) and Glutathione S-transferase (GST) activities than the control. Furthermore, the study detected that relative mRNA expression of Vitellogenin (Vg) and Vitellogenin receptor (VgR) for two treatments were lower than the control. In summary, two concentrations of spirodiclofen inhibited progeny growth and fecundity of <i>P. citri</i>. Additionally, the results of this study may support further research on tolerance of <i>P. citri</i> in response to spirodiclofen stress.</p>","PeriodicalId":9370,"journal":{"name":"Bulletin of Entomological Research","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142458591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and validation of reference genes for RT-qPCR analysis in <i>Sclerodermus guani</i> (Hymenoptera: Bethylidae).","authors":"Rina Zhao, Xiaomeng Guo, Ling Meng, Baoping Li","doi":"10.1017/S0007485324000427","DOIUrl":"https://doi.org/10.1017/S0007485324000427","url":null,"abstract":"<p><p>Gene expression studies in organisms are often conducted using reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR), and the accuracy of RT-qPCR results relies on the stability of reference genes. We examined ten candidate reference genes in <i>Sclerodermus guani</i>, a parasitoid wasp that is a natural enemy of long-horned beetle pests in forestry, including <i>ACT, EF1α, Hsc70, Hsp70, SRSF7, α-tubulin, RPL7A, 18S, 28S,</i> and <i>SOD1</i>, regarding variable biotic and abiotic factors such as body part, life stage, hormone, diet, and temperature. Data were analysed using four dedicated algorithms (ΔCt, BestKeeper, NormFinder, and geNorm) and one comparative tool (RefFinder). Our results showed that the most stable reference genes were <i>RPL7A</i> and <i>EF1α</i> regarding the body part, <i>SRSF7</i> and <i>Hsc70</i> regarding the diet, <i>RPL7A</i> and <i>α-tubulin</i> regarding the hormone, <i>SRSF7</i> and <i>RPL7A</i> regarding the life stage, and <i>SRSF7</i> and <i>α-tubulin</i> regarding temperature. To ascertain the applicability of specific reference genes, the expression level of the target gene (<i>ACPase</i>) was estimated regarding the body part using the most stable reference genes, <i>RPL7A</i> and <i>EF1α</i>, and the least stable one, <i>SOD1</i>. The highest expression level of <i>ACPase</i> was observed in the abdomen, and the validity of <i>RPL7A</i> and <i>EF1α</i> was confirmed. This study provides, for the first time, an extensive list of reliable reference genes for molecular biology studies in <i>S. guani</i>.</p>","PeriodicalId":9370,"journal":{"name":"Bulletin of Entomological Research","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2024-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142380082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of dietary restriction on development, mating, and reproduction in the natural predator <i>Pardosa pseudoannulata</i>.","authors":"Xiaoming Wang, Wei Li, Li Song, Zuojun Xie, Jie Liu, Yao Zhao, Yu Peng","doi":"10.1017/S0007485324000415","DOIUrl":"https://doi.org/10.1017/S0007485324000415","url":null,"abstract":"<p><p>Dietary restriction-influenced biological performance is found in many animal species. <i>Pardosa pseudoannulata</i> is a dominant spider species in agricultural fields and is important for controlling pests. In this study, three groups - a control group (CK group), a re-feeding group (RF group), and a dietary restriction group (RT group) - were used to explore development, mating, reproduction, and the expression levels of <i>Vg</i> (vitellogenin) and <i>VgR</i> (vitellogenin receptor) genes in the spider. The findings indicated that when subjected to dietary restriction, the carapace size, weight of the spiderlings, and weight of the adults exhibited a decrease. Furthermore, the preoviposition period and egg stage were observed to be prolonged, while the number of spiderlings decreased. It was also observed that re-feeding reduced cannibalism rates and extended the preoviposition period. Dietary restriction also affected the expression of the <i>Vg</i>-<i>3</i> gene in the spider. These results will contribute to the understanding of the impact of dietary restriction in predators of pest control, as well as provide a theoretical foundation for the artificial rearing and utilisation of the dominant spider in the field.</p>","PeriodicalId":9370,"journal":{"name":"Bulletin of Entomological Research","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2024-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142380083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Predicting the geographical potential distribution of species <i>Opisina arenosella</i> Walker in China under different climate scenarios based on the MaxEnt model.","authors":"Zhiling Wang, Zhihang Zhuo, Yaqin Peng, Danping Xu","doi":"10.1017/S0007485324000464","DOIUrl":"https://doi.org/10.1017/S0007485324000464","url":null,"abstract":"<p><p>As global warming increases with the frequency of extreme weather, the distribution of species is inevitably affected. Among them, highly damaging invasive species are of particular concern. Being able to effectively predict the geographic distribution of invasive species and future distribution trends is a key entry point for their control. <i>Opisina arenosella</i> Walker is an invasive species, and its ability to live on the backs of foliage and generate canals to hide adds to the difficulty of control. In this paper, the current and future distributions of <i>O. arenosella</i> under three typical emission scenarios in 2050 and 2090 are projected based on the MaxEnt model combining 19 bioclimatic variables. Filter through the variables to find the four key environment variables: BIO 1, BIO 6, BIO 11 and BIO 4. The results show that <i>O. arenosella</i> is distributed only in the eight provinces of Tibet, Yunnan, Fujian, Guangxi, Taiwan, Guangdong, Hong Kong and Hainan in the southeastern region. Its high suitability area is concentrated in Taiwan and Hainan. In the long run, highly suitable areas will continue to increase in size, while moderately suitable areas and poorly suitable areas will decrease to varying degrees. This paper aims to provide theoretical references for the control of <i>O. arenosella</i>.</p>","PeriodicalId":9370,"journal":{"name":"Bulletin of Entomological Research","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2024-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142361124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"NADPH-cytochrome P450 reductase involved in the lambda-cyhalothrin susceptibility on the green mirid bug <i>Apolygus lucorum</i>.","authors":"Congai Zhen, Rui Wu, Yao Tan, Ansheng Zhang, Lei Zhang","doi":"10.1017/S0007485324000488","DOIUrl":"https://doi.org/10.1017/S0007485324000488","url":null,"abstract":"<p><p>NADPH-cytochrome P450 reductase (CPR) is crucial for the detoxification process catalysed by cytochrome P450, which targets various exogenous xenobiotics, as well as pesticides. In our research, we successfully obtained the complete cDNA sequence of <i>Apolygus lucorum</i>'s CPR (<i>AlCPR</i>) using reverse transcription PCR along with rapid amplification of cDNA ends technology. Bioinformatics analysis exhibited that the inferred amino acid sequence of AlCPR is characteristic of standard CPRs, featuring an N-terminal membrane anchor and three conserved FMN, FAD and NADP binding sites. Phylogenetic result revealed that AlCPR was positioned within the Hemiptera cluster, showing a close evolutionary relationship with the CPR of <i>Cimex lectularius</i>. The real-time quantitative PCR results demonstrated widespread expression of <i>AlCPR</i> across various life stages and tissues of <i>A. lucorum</i>, with the most prominent expression in adults and the abdominal region. Injecting double-stranded RNA of <i>AlCPR</i> only significantly increased the lambda-cyhalothrin susceptibility in lambda-cyhalothrin-resistant strain rather than the susceptible strain. These findings suggest a potential link between AlCPR and the P450-dependent defence mechanism against lambda-cyhalothrin in <i>A. lucorum.</i></p>","PeriodicalId":9370,"journal":{"name":"Bulletin of Entomological Research","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2024-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142361123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}