Bulletin of Entomological Research最新文献

{"title":"Transcriptomic insights into Smoothened-dependent modulation of olfactory receptor genes in <i>Apis mellifera ligustica</i>: Implications for olfactory signal processing.","authors":"Lina Guo, Yu Zhang, Jue Wang, Diandian Yu, Yuan Guo","doi":"10.1017/S0007485325100229","DOIUrl":"https://doi.org/10.1017/S0007485325100229","url":null,"abstract":"<p><p>To investigate the effects of activating/inhibiting AmelSmo on the olfactory genes and signalling pathways of Apis mellifera ligustica, as well as the potential regulatory mechanisms involved. Transcriptomic sequencing was performed on Apis mellifera ligustica antennae using Illumina HiSeq platform following administration of cyclopamine (inhibitor) and purmorphamine (agonist). Differential gene expression analysis, coupled with GO and KEGG pathway annotations, facilitated the identification of olfactory receptor genes. The reliability of transcriptome data was subsequently validated through quantitative real-time-polymerase chain reaction analysis. Transcriptomic analysis revealed 12,356 differentially expressed genes (DEGs) between inhibitor and control groups, with 276 genes showing significant differential expression. Similarly, 12,356 DEGs were identified between the agonist and control groups, among which 672 genes exhibited significant differential expression. The GO annotation revealed that the DEGs in the inhibitor group and the agonist group were mainly enriched in the biological process such as cellular process, metabolic process, and biological regulation; in cellular component, enrichment was mainly observed in cell, cell part, and organelle; and in molecular function, the main enrichment was in binding and catalytic activity. KEGG pathway analysis indicated that DEGs from both groups were primarily enriched in signal transduction pathways. Among the DEGs, three olfactory receptor genes were identified in the inhibitor group: odorant receptor 19, odorant receptor 22, and odorant receptor 5. The agonist group exhibited two olfactory receptor genes: odorant receptor 109 and odorant receptor 26. All these olfactory receptor genes demonstrated downregulated expression patterns. Transcriptomic sequencing analysis identified five olfactory receptor genes. The changes in gene expression levels suggest that the activation or inhibition of AmelSmo may regulate the expression of olfactory receptors via the Hedgehog signalling pathway. It is speculated that AmelSmo may play a regulatory role in the olfactory system of bees.</p>","PeriodicalId":9370,"journal":{"name":"Bulletin of Entomological Research","volume":" ","pages":"1-9"},"PeriodicalIF":1.6,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144658470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potential use of the egg parasitoid <i>Trichogramma pretiosum</i> in the management of <i>Rachiplusia nu</i>.","authors":"Nathalia C Andrade, Weidson P Sutil, Viviane S Alves, Adeney de Freitas Bueno","doi":"10.1017/S0007485325100254","DOIUrl":"https://doi.org/10.1017/S0007485325100254","url":null,"abstract":"<p><p>Outbreaks of <i>Rachiplusia nu</i> have occurred on soybean in Brazil as the first species resistant to the <i>Bt</i> soybean expressing only Cry1Ac protein, triggering a significant increase in insecticide use on the crop. This threatens one of the most important benefits of adopting <i>Bt</i> soybean cultivars - the reduction of chemicals. Therefore, this research studied the biology and parasitism capacity of <i>Trichogramma pretiosum</i> at 20, 25, and 30 ± 2 °C on <i>R. nu</i> eggs in order to evaluate the potential of releasing this egg parasitoid in soybean to manage <i>R. nu</i>. Parasitoid exhibited high biological performance on the <i>R. nu</i> eggs as observed in the lifetime parasitism of 24.9, 46.4, and 34.4 <i>R. nu</i> eggs at 20, 25, and 30 °C, respectively, and 100% emergence in both biology and parasitism capacity experiments. The sex ratio was statistically lower at 20 °C (0.4947), but at all studied temperatures, the production of female descendants was equal (sex ratio of 0.4947 at 20 °C) or higher (sex ratio of 0.6666 at 25 °C and 0.6524 at 30 °C). All other evaluated parameters were similar to previously positive recorded observations for <i>T. pretiosum</i> on other soybean pests, such as <i>Anticarsia gemmatalis</i> and <i>Chrysodeixis includens</i>, against which the parasitoid has already been commercially released in the fields as a biocontrol option. Therefore, <i>T. pretiosum</i> might also be released in soybean as an egg parasitoid against <i>R. nu</i>, what needs to be confirmed in future field trials.</p>","PeriodicalId":9370,"journal":{"name":"Bulletin of Entomological Research","volume":" ","pages":"1-6"},"PeriodicalIF":1.6,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144648656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Host-parasitoid dynamics of <i>Pieris brassicae</i> and <i>Cotesia vestalis</i>: sustainable pest control in cruciferous crops.","authors":"Sadia Maalik, Sajida Mushtaq, Naheed Bano, Moazama Batool, Nazia Ehsan, Ayesha Hafeez, Mehmooda Munazir, Tauseef Anwar, Huma Qureshi, Muhammad Aqib Idrees, Wajid Zaman, Hameed Alsamadany, Khalid H Alamer, Nazih Y Rebouh, Konul Ahmadova","doi":"10.1017/S0007485325100187","DOIUrl":"10.1017/S0007485325100187","url":null,"abstract":"<p><p>Pieris brassicae (Linnaeus, 1758) (Lepidoptera: Pieridae), commonly known as the cabbage butterfly, is a major herbivorous pest causing significant damage to Brassica crops, which are widely cultivated for edible parts and economic uses such as oilseed production. Conventional chemical-based pest control methods pose risks to environmental and human health, prompting interest in sustainable alternatives like biological control using parasitoids. This study evaluated crop damage by P. brassicae larvae and the potential of the larval parasitoid Cotesia vestalis (Haliday, 1834) (Hymenoptera: Braconidae) in managing pest populations. Larvae and fertilized eggs of P. brassicae were collected and reared on four host diets (cabbage, cauliflower, turnip, and generic brassica) under controlled laboratory conditions. Larval growth, feeding behavior, and development were assessed, along with a feeding index for dietary efficiency. Parasitisation was introduced by exposing larvae to adult C. vestalis, and host-parasitoid interactions were statistically analyzed using one-way ANOVA and regression models. Results revealed that larvae had the highest feeding preference and weight gain on brassica, while turnip-fed larvae consumed the least. Parasitisation by C. vestalis significantly suppressed larval development, with a strong correlation between parasitisation rates and reduced host fitness. These findings highlight C. vestalis as a promising biological control agent for P. brassicae, offering an effective and eco-friendly alternative to chemical pesticides. Further research should focus on optimizing C. vestalis mass-rearing protocols and release strategies tailored to diverse Brassica cropping systems to promote sustainable pest management.</p>","PeriodicalId":9370,"journal":{"name":"Bulletin of Entomological Research","volume":" ","pages":"1-9"},"PeriodicalIF":1.6,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144599460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potential risks of glyphosate-based herbicide spraying on parental and offspring generations of <i>Chrysopa pallens</i> (Neuroptera: Chrysopidae).","authors":"Qiankun Du, Dan Wang, Xianpeng Song, Hongyan Hu, Yongpan Shan, Changcai Wu, Yajie Ma, Xiangliang Ren, Yan Ma","doi":"10.1017/S0007485325100175","DOIUrl":"10.1017/S0007485325100175","url":null,"abstract":"<p><p>Glyphosate, the most prevalent pesticide and widely used herbicide globally, has seen much research on its potential ecological toxicity. Glyphosate-based herbicide (GBH) is directly sprayed in the field, exposing predators to the chemical through contaminated prey or direct contact. While the consequences of ingesting glyphosate have been explored, the specific impact of GBH spraying on <i>Chrysopa pallens</i> (Neuroptera: Chrysopidae) remains unclear. In this study, life tables were constructed to evaluate the potential effects of different stages of exposure to GBH on both the parents (F₀) and offspring (F₁) of <i>C. pallens</i>, and the expression of genes related to the insulin signalling pathway and vitellogenin (<i>Vg1</i>) was detected by reverse transcription-quantitative polymerase chain reaction. The results revealed that medium (10 mL/L) and high (20 mL/L) concentrations of GBH adversely affected the development and longevity of the F₀ and F₁ generation of <i>C. pallens</i> larvae. Notably, high concentrations of GBH significantly reduced the fecundity of the F₀ and suppressed <i>Vg1</i> transcription at both medium and high concentrations. While GBH treatment of <i>C. pallens</i> adults showed no harmful effects on the longevity, fecundity, population parameters, and the transcription levels of genes involved in insulin signalling and <i>Vg1</i> in the F₀. Nevertheless, it altered the developmental duration of the F₁. Therefore, spraying GBH may lead to reduced fecundity and inhibit the <i>Vg1</i> transcription, posing potential risks to both parental and offspring generations of <i>C. pallens</i>. These findings offer valuable insights into the proper utilisation of GBH.</p>","PeriodicalId":9370,"journal":{"name":"Bulletin of Entomological Research","volume":" ","pages":"1-11"},"PeriodicalIF":1.6,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144574870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Temporal and spatial patterns of <i>Bactrocera dorsalis</i> (Diptera: Tephritidae) populations in its southern limits of distribution: effects of climate and landscape on its range expansion.","authors":"Aruna Manrakhan, Eleni Verykouki, Leani Serfontein, Eitan Goldshtein, Rooikie R Beck, Darren J Kriticos, Anna M Szyniszewska, Karol Kozyra, Nikolaos T Papadopoulos, David Nestel","doi":"10.1017/S0007485325100230","DOIUrl":"10.1017/S0007485325100230","url":null,"abstract":"<p><p><i>Bactrocera dorsalis</i> (Hendel) (Diptera: Tephritidae) is an invasive tropical pest that is currently expanding in its geographical range into temperate regions. This study examined the temporal and spatial dynamics of <i>B. dorsalis</i> along an altitudinal gradient in Mpumalanga Province, South Africa, in its southernmost adventive limit, which experiences a temperate climate with dry winters. Populations were monitored from October 2020 to October 2022 at three sites with altitudes ranging from 452 to 1 741 m above sea level. At each site, clusters of attractant-based traps (methyl eugenol and three-component Biolure) were set up and serviced at least monthly. We analysed the effects of climate, time of the year, altitude, and landscape on <i>B. dorsalis</i> abundance. Single population peaks of <i>B. dorsalis</i> were recorded from mid-summer to autumn in all sites, with higher prevalence at the low-altitude site. In the low- and mid-altitude sites, catches were recorded year-round, while at the high-altitude site, there were no catches for four to six months after onset of winter. Higher <i>B. dorsalis</i> catches were recorded as temperatures increased and precipitation decreased. Catches were higher in commercial orchards and home gardens compared to abandoned orchards. These findings provide valuable information for improving simulation models of <i>B. dorsalis</i> distribution and population growth that can be used to inform the management of this pest.</p>","PeriodicalId":9370,"journal":{"name":"Bulletin of Entomological Research","volume":" ","pages":"1-16"},"PeriodicalIF":1.6,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144574871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The synergistic effect of <i>Metarhizium anisopliae</i> CQMa421 and deltamethrin improved the insecticidal ability against to <i>Bactrocera dorsalis</i>.","authors":"Jinyuan Zhao, Chen Shen, Yangjunlu Shen, Lijuan Liu, Wenkai Yan, Yujia Guan, Renwen Zheng, Sihan Lu, Qingfeng Tang","doi":"10.1017/S0007485325100217","DOIUrl":"10.1017/S0007485325100217","url":null,"abstract":"<p><p><i>Bactrocera dorsalis</i> (Diptera: Tephritidae) is a highly invasive and destructive quarantine pests worldwide. To improved biological control efficiency, reduce chemical pesticides use, and optimise the application of <i>Metarhizium anisopliae</i> (Hypocreales: Clavicipitaceae) against <i>B. dorsalis</i>. This study evaluated the combined toxicity of <i>M. anisopliae</i> with deltamethrin and chlorpyrifos. The biocompatibility of <i>M. anisopliae</i> CQMa421 with these pesticides was assessed based on spore germination, mycelial growth, and sporulation. Additionally, the effects of combined treatments on detoxification enzyme and related gene expression in <i>B. dorsalis</i> were investigated. The results indicated that the virulence effect of <i>M. anisopliae</i> CQMa421 against <i>B. dorsalis</i> adults was time-dependent and dose-dependent. Deltamethrin showed good compatibility with <i>M. anisopliae</i> CQMa421, achieving 100% mortality at 1 × 10⁸ CFU/mL by 84 hours. Different concentrations of deltamethrin can promote the mycelial growth and sporulation of <i>M. anisopliae</i> CQMa421. The toxicity effect of deltamethrin and chlorpyrifos combined with <i>M. anisopliae</i> CQMa421 on <i>B. dorsalis</i> adults was better than that of single-agent treatment, and the co-toxicity factor of 5 mg/L deltamethrin and 1 × 10⁸ CFU/mL <i>M. anisopliae</i> CQMa421 was 24.81, which synergistically affected on <i>B. dorsalis</i> control. Enzyme activity assays and qRT-PCR results revealed that the combination treatment differentially activated and enhanced the activities of AChE, CarE, GST, CAT, and SOD. Meanwhile, <i>BdCarE</i> was significantly inhibited and upregulating <i>BdGSTD7, BdGSTS1, BdCYP4ae</i>1, <i>BdPOD, BdPOD1</i>, and <i>BdCAT</i> genes. In conclusion, the combination of deltamethrin and <i>M. anisopliae</i> CQMa421 enhanced the insecticidal efficacy against <i>B. dorsalis</i>, significantly affected the activity of related detoxification enzymes. Provided a robust basis for integrating biological and chemical control strategies to manage <i>B. dorsalis</i> more effectively.</p>","PeriodicalId":9370,"journal":{"name":"Bulletin of Entomological Research","volume":" ","pages":"1-9"},"PeriodicalIF":1.6,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144552314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transgenerational effect of <i>Cordyceps fumosorosea</i> (Wize) on demographic parameters and detoxifying enzymes of <i>Spodoptera frugiperda</i> (Smith).","authors":"Samina Rafique, Shoaib Freed, Hira Sabir, Momina Azhar, Afifa Naeem, Rizwan Ahmed","doi":"10.1017/S0007485325100242","DOIUrl":"10.1017/S0007485325100242","url":null,"abstract":"<p><p><i>Spodoptera frugiperda</i> is a notorious pest that has been recorded attacking over 353 crop species worldwide. Excessive insecticide exposure can lead to resistance and has adverse impacts on the environment and beneficial organisms. Long-lasting pest control methods like entomopathogenic fungi may be used to prevent the negative impact of synthetic insecticides. In the current research, effectiveness of <i>Cordyceps fumosorosea</i> was analysed by applying a sub-lethal dose (LC₁₅ = 2.09 × 10⁶ spores mL^-1) and a lethal dose (LC₅₀ = 2.17 × 10⁷ spores mL^-1) on filial (F₀) and first filial (F₁) generations of <i>S. frugiperda</i> to estimate both lethal and sub-lethal effects. The LC₁₅ was used to keep a significant proportion of larvae alive to permit determination of sublethal effects on <i>S. frugiperda.</i> After treatment with the LC₁₅ and LC₅₀, <i>S. frugiperda</i> progeny displayed shorter larval duration, lower fecundity, and shorter adult female and male longevities relative to untreated controls. Likewise, the adult pre-oviposition period, total pre-oviposition period, oviposition days (<i>O_d</i>), net reproductive rates (<i>Ro</i>), and mean generation times (<i>T</i>) were reduced in fungal-infected groups. Furthermore, activities of key detoxifying enzymes, i.e. acetylcholinesterase (AChE), glutathione <i>S</i>-transferases (GST), and esterases (EST), were also evaluated. Substantial differences of AChE, GST, and EST (24.3, 18.34, and 10.09 µmol/min/mg protein), respectively, were observed at the LC₅₀ in contrast to the LC₁₅ and controls. The current study showed pathogenicity of <i>C. fumosorosea</i>, which negatively affected insect development and changed the activities of detoxifying enzymes, thereby increasing the effectiveness and eco-friendly management of <i>S. frugiperda</i>.</p>","PeriodicalId":9370,"journal":{"name":"Bulletin of Entomological Research","volume":" ","pages":"1-12"},"PeriodicalIF":1.6,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144539052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterisation and expression analysis of extracellular superoxide dismutases of <i>Protohermes xanthodes</i> navás (Megaloptera: Corydalidae) in response to sublethal chlorpyrifos exposure.","authors":"Xi Wen, Yun Lu, Jie Yang, Yue Zhang, Xingrui Huang, Jie Zou, Mengqing Zhang, Xihui Wang, Wei Zhao, Xinglong Huang, Zhengwei Wu","doi":"10.1017/S0007485325100151","DOIUrl":"10.1017/S0007485325100151","url":null,"abstract":"<p><p>The extracellular matrices, such as the haemolymph, in insects are at the centre of most physiological processes and are protected from oxidative stress by the extracellular antioxidant enzymes. In this study, we identified two secreted superoxide dismutase genes (<i>PxSOD3</i> and <i>PxSOD5</i>) and investigated the oxidative stress induced by chlorpyrifos (CPF) in the aquatic insect <i>Protohermes xanthodes</i> (Megaloptera: Corydalidae). PxSOD3 and PxSOD5 contain the signal peptides at the N-terminus. Structure analysis revealed that PxSOD3 and PxSOD5 contain the conserved CuZn-SOD domain, which is mainly composed of β-sheets and has conserved copper and zinc binding sites. Both PxSOD3 and PxSOD5 are predicted to be soluble proteins located in the extracellular space. After exposure to different concentrations of sublethal CPF, MDA content in <i>P. xanthodes</i> larvae were increased in a dose-dependent manner; SOD and CAT activities were also higher in CPF-treated groups than that in the no CPF control, indicating that sublethal CPF induces oxidative stress in <i>P. xanthodes</i> larvae. Furthermore, <i>PxSOD3</i> and <i>PxSOD5</i> expression levels and haemolymph SOD activity in the larvae were downregulated by sublethal CPF at different concentrations. Our results suggest that the PxSOD3 and PxSOD5 are putative extracellular antioxidant enzymes that may play a role in maintaining the oxidative balance in the extracellular space. Sublethal CPF may induce oxidative stress in the extracellular space of <i>P. xanthodes</i> by reducing the gene expression and catalytic activity of extracellular SODs.</p>","PeriodicalId":9370,"journal":{"name":"Bulletin of Entomological Research","volume":" ","pages":"1-9"},"PeriodicalIF":1.6,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144526509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inheritance patterns of resistance to sodium channel blocker insecticides in <i>Spodoptera frugiperda</i> (Lepidoptera: Noctuidae).","authors":"Ingrid Schimidt Kaiser, Aline Sartori Guidolin, Rubens Hideo Kanno, Fernando Semmelroth de Assunção E Amaral, Carolina Pacchioni Monteiro, Celso Omoto","doi":"10.1017/S0007485325100163","DOIUrl":"10.1017/S0007485325100163","url":null,"abstract":"<p><p>The use of sodium channel blocker insecticides (SCBIs) has been one of the tools for managing the resistance of fall armyworm <i>Spodoptera frugiperda</i> (J.E. Smith, 1797) (Lepidoptera: Noctuidae) to insecticides. In this study, we selected resistant strains of <i>S. frugiperda</i> to the SCBIs indoxacarb (Indoxacarb-R) and metaflumizone (Metaflumizone-R), under laboratory conditions, to evaluate the inheritance of resistance, cross-resistance to insecticides targeting voltage-gated sodium channels, and verify the absence of the F1845Y and V1848I mutations. The LC₅₀ values of the susceptible (SUS) and the Indoxacarb-R strains to indoxacarb were 3.72 and 114.43 µg mL^-1 respectively, and for the SUS and the Metaflumizone-R strains to metaflumizone were 4.57 and 3,141.96 µg mL^-1, respectively, with resistance ratios of approximately 30-fold to indoxacarb and >600-fold to metaflumizone. The resistance of <i>S. frugiperda</i> to both insecticides was characterised as autosomal, incompletely recessive, and polygenic. Cross-resistance between indoxacarb and metaflumizone was detected. Moreover, Indoxacarb-R and Metaflumizone-R strains showed lower susceptibility to the pyrethroid insecticide lambda-cyhalothrin, possibly due to multiple resistance. The partial sequencing of the <i>S. frugiperda</i> sodium channel gene did not confirm the association of F1845Y and V1848I mutations with <i>S. frugiperda</i> resistance to indoxacarb and metaflumizone. These results will be important for implementing proactive insect resistance management programmes to preserve the lifetime of SCBIs in controlling <i>S. frugiperda</i>.</p>","PeriodicalId":9370,"journal":{"name":"Bulletin of Entomological Research","volume":" ","pages":"1-10"},"PeriodicalIF":1.6,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144526510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High flavonoid-producing tomato reduces whitefly phloem-feeding efficiency by inducing reactive oxygen species accumulation and callose deposition.","authors":"Yujie Li, Tianyu Huang, Fengbo Yang, Rong Zhang, Weina Gu, Peng Lu, Hong Tong, Yuting Yang, Zhixiong Zhou, Qingjun Wu, Youjun Zhang, Qi Su","doi":"10.1017/S0007485325100205","DOIUrl":"https://doi.org/10.1017/S0007485325100205","url":null,"abstract":"<p><p>The whitefly <i>Bemisia tabaci</i> (Hemiptera: Aleyrodidae) is economically one of the most threatening pests in tomato cultivation, which not only causes direct damage but also transmits many viruses. Breeding whitefly-resistant tomato varieties is a promising and environmentally friendly method to control whitefly populations in the field. Accumulating evidence from tomato and other model systems demonstrates that flavonoids contribute to plant resistance to herbivorous insects. Previously, we found that high flavonoid-producing tomato line deterred whitefly oviposition and settling behaviours, and was more resistant to whiteflies compared to the near-isogenic low flavonoid-producing tomato line. The objective of the current work is to describe in detail different aspects of the interaction between the whitefly and two tomato lines, including biochemical processes involved. Electrical penetration graph recordings showed that high flavonoid-producing tomato reduced whitefly probing and phloem-feeding efficiency. We also studied constitutive and induced plant defence responses and found that whitefly induced stronger reactive oxygen species accumulation through NADPH oxidase in high flavonoid-producing tomato than in low flavonoid-producing tomato. Moreover, whitefly feeding induced the expression of callose synthase genes and resulted in callose deposition in the sieve elements in high flavonoid-producing tomato but not in low flavonoid-producing tomato. As a consequence, whitefly feeding on high flavonoid-producing tomato significantly decreased uptake of phloem and reduced its performance when compared to low flavonoid-producing tomato. These results indicate that high flavonoid-producing tomato provides phloem-based resistance against whitefly infestation and that the breeding of such resistance in new varieties could enhance whitefly management.</p>","PeriodicalId":9370,"journal":{"name":"Bulletin of Entomological Research","volume":" ","pages":"1-9"},"PeriodicalIF":1.6,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144504889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}