Journal of chromatography open最新文献

{"title":"Optimization of the ionic liquid-based dispersive liquid–liquid microextraction combined with high-performance liquid chromatography coupled with diode array detection for the determination of multiclass pesticide residues in water samples","authors":"Bezuayehu Tadesse Negussie ,&nbsp;Simiso Dube ,&nbsp;Mathew Muzi Nindi ,&nbsp;Asmamaw Tesfaw","doi":"10.1016/j.jcoa.2025.100231","DOIUrl":"10.1016/j.jcoa.2025.100231","url":null,"abstract":"<div><div>An expeditious and effective analytical methodology, predicated on ionic liquid-based dispersive liquid–liquid microextraction (IL-DLLME) integrated with high-performance liquid chromatography coupled with diode array detection (HPLC-DAD), has been devised for the quantification of multiclass pesticides in aquatic samples. This methodology employs 1-Hexyl-3-methylimidazolium hexafluorophosphate ([C₁₀H₁₉N₂][PF₆]), 1-Octyl-3-methylimidazolium hexafluorophosphate ([C₁₂H₂₃N₂][PF₆]), and 1‑butyl‑3-methylimidazolium hexafluorophosphate ([C₈H₁₅N₂][PF₆]) as the extraction solvent and utilizes methanol as the disperser solvent. Critical parameters influencing the efficacy of the extraction, encompassing the type and volume of extraction and disperser solvents, sample pH, and vortex conditions, were meticulously optimized. Under optimal conditions, the methodology exhibited commendable linearity (0.9974 ≤ R² ≤ 0.9996), minimal limits of detection (0.1–1.3 µg/L) and quantification (0.3–3.9 µg/L), as well as satisfactory precision (relative standard deviation (RSD) ≤ 9.6 %). The methodology was adeptly applied to the analysis of tap water, groundwater, and river water samples, yielding recoveries spanning from 85 % to 105 %. The findings substantiate that IL-DLLME represents an auspicious technique for the extraction and quantification of multiclass pesticides in intricate aqueous matrices.</div></div>","PeriodicalId":93576,"journal":{"name":"Journal of chromatography open","volume":"8 ","pages":"Article 100231"},"PeriodicalIF":0.0,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144272211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “From multimodal liquid chromatography to supercritical fluid chromatography: Mapping chiral separation of the major organic ultraviolet filters” [J. Chromatogr. Open 8 (2025) 100251]","authors":"Leandro Oka-Duarte ,&nbsp;Giovanni S. Baviera ,&nbsp;Weston J. Umstead ,&nbsp;Quezia B. Cass ,&nbsp;Anderson R.M. de Oliveira","doi":"10.1016/j.jcoa.2025.100283","DOIUrl":"10.1016/j.jcoa.2025.100283","url":null,"abstract":"","PeriodicalId":93576,"journal":{"name":"Journal of chromatography open","volume":"8 ","pages":"Article 100283"},"PeriodicalIF":3.2,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145693502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Liquid chromatography coupled to mass spectrometry for steroid hormones analysis: issues and solutions in sample preparation and method development","authors":"A. Temerdashev ,&nbsp;S. Girel ,&nbsp;S.N. Atapattu ,&nbsp;Y.-Q. Feng ,&nbsp;E. Gashimova ,&nbsp;T. Malitskaya ,&nbsp;I. Podolskiy ,&nbsp;Q.-F. Zhu","doi":"10.1016/j.jcoa.2025.100250","DOIUrl":"10.1016/j.jcoa.2025.100250","url":null,"abstract":"<div><div>Current work presents main aspects of the application of liquid chromatography in combination with low- and high-resolution mass spectrometry to an analysis of steroid hormones. Advantages and challenges of both targeted and untargeted analysis are shown together with the most popular approaches to the associated sample preparation. Among emerging approaches, first applications of isotope ratio mass spectrometry in combination with liquid chromatography for the analysis of steroid hormones for doping control purposes are presented and discussed.</div></div>","PeriodicalId":93576,"journal":{"name":"Journal of chromatography open","volume":"8 ","pages":"Article 100250"},"PeriodicalIF":3.2,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145049340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative study of pesticide analysis in river water by gas chromatography/mass spectrometry screening and standard methods","authors":"Norihiro Kobayashi ,&nbsp;Yuko Tsuchiya ,&nbsp;Sumomo Nakamura ,&nbsp;Kenta Nohara ,&nbsp;Takako Yamazaki ,&nbsp;Tomoko Oda ,&nbsp;Teruaki Kinoshita","doi":"10.1016/j.jcoa.2025.100285","DOIUrl":"10.1016/j.jcoa.2025.100285","url":null,"abstract":"<div><div>This study entailed a comparative analysis of the standard method (notification method) and the screening method based on gas chromatography/mass spectrometry (GC/MS) for detecting 168 pesticides. To clarify the qualitative and quantitative accuracy of the GC/MS screening method, the occurrence of pesticides in river water samples was determined using both methods. The lowest concentration in the calibration curves for 155 pesticides was 0.01 mg/L, corresponding to a limit of quantification (LOQ) of 0.01 μg/L in river water after accounting for the 1000-fold concentration factor from sample pretreatment. Except for two cases wherein the chromatogram showed interference peaks, both methods yielded consistent identification results. The quantitative values obtained by the screening method were 0.52–1.45 times (quantitative error ranging from −48% to +45%) those obtained by the notification method. The quantitative error associated with the screening method decreased significantly with increasing quantitative values. Calibration curves for each pesticide were prepared five times throughout the study period. Upon comparing with the initial calibration curve, the changes in these calibration curve slopes generally vary between −50% and +100% for each pesticide. Quantitative accuracy could be ensured by comprehensively maintaining the instrument conditions, even during prolonged use. This indicates that the quantitative errors of the screening method are controllable, although they are not equivalent to those of the notification method. Therefore, the GC/MS screening method can be effectively utilized for water quality management. To avoid underestimation of pesticide concentrations, the GC/MS screening method values may be multiplied by a safety factor of 2.</div></div>","PeriodicalId":93576,"journal":{"name":"Journal of chromatography open","volume":"8 ","pages":"Article 100285"},"PeriodicalIF":3.2,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145578665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enantiomeric separation of 1-methyl-tryptophan on a mixed-mode stationary phase via pre-column derivatization and in vivo assessment of chiral inversion in rats","authors":"Mayu Onozato,&nbsp;Syuntei Fu,&nbsp;Tomoya Takaura,&nbsp;Tatsuya Sakamoto,&nbsp;Takeshi Fukushima","doi":"10.1016/j.jcoa.2025.100257","DOIUrl":"10.1016/j.jcoa.2025.100257","url":null,"abstract":"<div><div>1-Methyl-<span>d</span>-tryptophan (<span>d</span>-MT) is an inhibitor of the tryptophan (Trp)-catabolizing enzyme indoleamine 2,3-dioxygenase (IDO). <span>d</span>-MT has been used in studies on Trp metabolism inhibition and as a concomitant drug with antitumor agents. In this study, we investigated the enantiomeric separation of <span>d</span>-MT and its enantiomer, <span>l</span>-MT, via LC-MS/MS following pre-column derivatization with succinimidyl 2-(3-((benzyloxy)carbonyl)-1-methyl-5-oxoimidazolidin-4-yl) acetate ((<em>R</em>)-CIMa-OSu). Consequently, the use of a mixed-mode stationary phase, Scherzo^Ⓡ SM-C18, provided a separation factor (<em>α</em>) of 1.44 and a resolution (<em>R</em>_s) of 6.14, with both values being higher than those obtained with a reversed-phase column (<em>α</em>: 1.29, <em>R</em>_s: 2.69). Using the proposed LC-MS/MS method, the time-course profiles of plasma <span>d</span>-MT concentrations in rats administered <span>d</span>-MT were investigated. Consequently, a chiral inversion of <span>d</span>-MT to <span>l</span>-MT was observed in rats administered <span>d</span>-MT, whereas no inversion of <span>l</span>-MT to <span>d</span>-MT was observed in rats administered <span>l</span>-MT. The involvement of <span>d</span>-amino acid oxidase (DAO) in the chiral inversion of <span>d</span>-MT to <span>l</span>-MT was suggested, as pre-administration of DAO inhibitors significantly increased the <span>d</span>-MT concentrations in rat plasma.</div></div>","PeriodicalId":93576,"journal":{"name":"Journal of chromatography open","volume":"8 ","pages":"Article 100257"},"PeriodicalIF":3.2,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145157315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A thermodynamic study of relative extractability of ethanol to blood simulating solvent in extractables and leachables analysis of medical devices by Abraham’s Solvation Parameter Model","authors":"Jianwei Li","doi":"10.1016/j.jcoa.2025.100238","DOIUrl":"10.1016/j.jcoa.2025.100238","url":null,"abstract":"&lt;div&gt;&lt;div&gt;A thermodynamic study of over-extraction of blood contacting medical devices by ethanol to ethanol/water binary cosolvent mixture (50/50, v/v), as a blood simulating solvent, in chemical analysis of medical devices is evaluated by Abraham’s solvation parameter model using five representative materials (low density polyethylene or LDPE, silicone, polyurethane or PU, polyoxymethylene or POM, and polyacrylate or PA). The Abraham’s model is initially used to calculate the material-solvent partition system coefficients by the corresponding partition system constants and representative extractables compounds between five materials and ethanol/water (and methanol/water) cosolvent mixtures at different volume fractions. The partition system constants are indirectly derived by a “thermodynamic circle conversion” method, based on material-water partition systems and solvent-water water partition systems. The material-solvent (mixture) partition coefficient, &lt;span&gt;&lt;math&gt;&lt;msub&gt;&lt;mi&gt;P&lt;/mi&gt;&lt;mrow&gt;&lt;mi&gt;M&lt;/mi&gt;&lt;mo&gt;/&lt;/mo&gt;&lt;mi&gt;S&lt;/mi&gt;&lt;mi&gt;o&lt;/mi&gt;&lt;mi&gt;l&lt;/mi&gt;&lt;mi&gt;v&lt;/mi&gt;&lt;mi&gt;e&lt;/mi&gt;&lt;mi&gt;n&lt;/mi&gt;&lt;mi&gt;t&lt;/mi&gt;&lt;/mrow&gt;&lt;/msub&gt;&lt;/math&gt;&lt;/span&gt;=&lt;span&gt;&lt;math&gt;&lt;mrow&gt;&lt;msub&gt;&lt;mi&gt;C&lt;/mi&gt;&lt;mi&gt;M&lt;/mi&gt;&lt;/msub&gt;&lt;mo&gt;/&lt;/mo&gt;&lt;msub&gt;&lt;mi&gt;C&lt;/mi&gt;&lt;mrow&gt;&lt;mi&gt;S&lt;/mi&gt;&lt;mi&gt;o&lt;/mi&gt;&lt;mi&gt;l&lt;/mi&gt;&lt;mi&gt;v&lt;/mi&gt;&lt;mi&gt;e&lt;/mi&gt;&lt;mi&gt;n&lt;/mi&gt;&lt;mi&gt;t&lt;/mi&gt;&lt;/mrow&gt;&lt;/msub&gt;&lt;/mrow&gt;&lt;/math&gt;&lt;/span&gt;, defined as the concentration in the material phase divided by the concentration in the solvent phase, is used as an indicator of the solvent extraction strength. &lt;span&gt;&lt;math&gt;&lt;mrow&gt;&lt;mi&gt;log&lt;/mi&gt;&lt;mo&gt;(&lt;/mo&gt;&lt;msub&gt;&lt;mi&gt;P&lt;/mi&gt;&lt;mrow&gt;&lt;mi&gt;M&lt;/mi&gt;&lt;mo&gt;/&lt;/mo&gt;&lt;mi&gt;S&lt;/mi&gt;&lt;mi&gt;o&lt;/mi&gt;&lt;mi&gt;l&lt;/mi&gt;&lt;mi&gt;v&lt;/mi&gt;&lt;mi&gt;e&lt;/mi&gt;&lt;mi&gt;n&lt;/mi&gt;&lt;mi&gt;t&lt;/mi&gt;&lt;/mrow&gt;&lt;/msub&gt;&lt;mo&gt;)&lt;/mo&gt;&lt;/mrow&gt;&lt;/math&gt;&lt;/span&gt; values are computed for all material-solvent pairs using the representative extractables compounds, mostly from Wayne State University experimental descriptor database (WSUEDD). The predictive &lt;span&gt;&lt;math&gt;&lt;mrow&gt;&lt;msub&gt;&lt;mi&gt;log&lt;/mi&gt;&lt;mn&gt;10&lt;/mn&gt;&lt;/msub&gt;&lt;mrow&gt;&lt;mo&gt;(&lt;/mo&gt;&lt;msub&gt;&lt;mi&gt;P&lt;/mi&gt;&lt;mrow&gt;&lt;mi&gt;M&lt;/mi&gt;&lt;mo&gt;/&lt;/mo&gt;&lt;mi&gt;S&lt;/mi&gt;&lt;mi&gt;o&lt;/mi&gt;&lt;mi&gt;l&lt;/mi&gt;&lt;mi&gt;v&lt;/mi&gt;&lt;mi&gt;e&lt;/mi&gt;&lt;mi&gt;n&lt;/mi&gt;&lt;mi&gt;t&lt;/mi&gt;&lt;/mrow&gt;&lt;/msub&gt;&lt;mo&gt;)&lt;/mo&gt;&lt;/mrow&gt;&lt;/mrow&gt;&lt;/math&gt;&lt;/span&gt; values between LDPE and silicone materials and ethanol/water (and methanol/water) cosolvent mixtures are compared with the available experimental values to assess the model’s predictive accuracy. Afterward, the predictive &lt;span&gt;&lt;math&gt;&lt;mrow&gt;&lt;msub&gt;&lt;mi&gt;log&lt;/mi&gt;&lt;mn&gt;10&lt;/mn&gt;&lt;/msub&gt;&lt;mrow&gt;&lt;mo&gt;(&lt;/mo&gt;&lt;msub&gt;&lt;mi&gt;P&lt;/mi&gt;&lt;mrow&gt;&lt;mi&gt;M&lt;/mi&gt;&lt;mo&gt;/&lt;/mo&gt;&lt;mi&gt;S&lt;/mi&gt;&lt;mi&gt;o&lt;/mi&gt;&lt;mi&gt;l&lt;/mi&gt;&lt;mi&gt;v&lt;/mi&gt;&lt;mi&gt;e&lt;/mi&gt;&lt;mi&gt;n&lt;/mi&gt;&lt;mi&gt;t&lt;/mi&gt;&lt;/mrow&gt;&lt;/msub&gt;&lt;mo&gt;)&lt;/mo&gt;&lt;/mrow&gt;&lt;/mrow&gt;&lt;/math&gt;&lt;/span&gt; values of ethanol to ethanol/water binary cosolvent mixture (50/50, v/v) are used to calculate relative extractability (&lt;em&gt;RE&lt;/em&gt;), as an indicator of over-extraction. Several conclusions can be drawn from this study. First, the predicted partition coefficients are confirmed by available experimental values (LDPE and silicone). Second, the over-extraction of medical devices by ethanol to the blood simulating solvent is less important ","PeriodicalId":93576,"journal":{"name":"Journal of chromatography open","volume":"8 ","pages":"Article 100238"},"PeriodicalIF":0.0,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144655274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Full comprehensive ion-pair-mediated anion exchange chromatography × ion-pair reversed-phase liquid chromatography fingerprinting of digested messenger ribonucleic acid drug substances","authors":"Niklas Carstensen ,&nbsp;Ryan Karongo ,&nbsp;Anne Mengel ,&nbsp;Michael Lämmerhofer","doi":"10.1016/j.jcoa.2025.100271","DOIUrl":"10.1016/j.jcoa.2025.100271","url":null,"abstract":"<div><div>The growing interest in messenger RNA (mRNA) based therapeutics has increased the need for analytical method development to meet the desired standards for quality control of these novel drugs. The identity of the mRNA drug substance is usually confirmed via indirect approaches such as Sanger sequencing or next generation sequencing (NGS), or more direct approaches such as LC-MS/MS after digestion with specific RNases. These conventional bottom-up MS methodologies, however, usually require several partial digests with various RNases to get sufficient sequence coverage. In the present study, a comprehensive two-dimensional liquid chromatography (LC × LC) platform is presented, utilizing ion-pair-mediated anion exchange chromatography in the first dimension and ion-pair reversed-phase liquid chromatography in the second dimension and evaluated using the convex hull model for orthogonality determination. This platform can be employed for the fingerprinting of RNase T1 digested mRNA, enabling the distinction between different mRNAs based on the fingerprint of a full digestion with a single RNase. It could also be used to confirm its identity or help with sequencing efforts, when combined with high-resolution MS.</div></div>","PeriodicalId":93576,"journal":{"name":"Journal of chromatography open","volume":"8 ","pages":"Article 100271"},"PeriodicalIF":3.2,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145361931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in miniaturized liquid sample preparation techniques for environmental analysis: A special look towards green solvents and automation","authors":"Rafael Oliveira Martins,&nbsp;Camila Will,&nbsp;Maria Flávia Assunção Magalhães,&nbsp;Fernando Mauro Lanças","doi":"10.1016/j.jcoa.2025.100240","DOIUrl":"10.1016/j.jcoa.2025.100240","url":null,"abstract":"<div><div>The continuous advancements in agricultural and industrial practices have led to the widespread introduction of various chemicals across key production sectors. While these substances offer significant benefits, their improper disposal has led to their accumulation in environmental compartments, raising global concerns about their potential impacts on ecosystems and human health. In quantitative and qualitative analysis, liquid miniaturized techniques have been widely applied to these matrices to ensure the detection and quantification of these compounds in environmental samples. Many of their advantages are rooted in key principles of green analytical chemistry, such as reduced sample and solvent consumption, as well as their versatility in enabling diverse extraction designs. However, the use of conventional organic solvents and labor-intensive extraction procedures still limits the full potential of these techniques in environmental applications. Hence, the present review provides an overview of current applications of miniaturized liquid-phase extraction protocols in environmental analysis, highlighting innovative approaches. Moreover, we discuss the use of green solvents and the potential for automation in these methods. Through this review, we aim to encourage researchers to adopt miniaturized liquid techniques for environmental monitoring, thereby advancing the implementation of more sustainable and efficient analytical strategies.</div></div>","PeriodicalId":93576,"journal":{"name":"Journal of chromatography open","volume":"8 ","pages":"Article 100240"},"PeriodicalIF":0.0,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144596592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid screening and identification of influenza virus polymerase inhibitors from Rubus idaeus L. by at-line nanofractionation","authors":"Zhixu Chen ,&nbsp;Rongkai Gu ,&nbsp;Mingming Zhao ,&nbsp;Kefeng Linghu ,&nbsp;Huanwei Fan ,&nbsp;Tingting Zhang ,&nbsp;Jincai Wang ,&nbsp;Zhengjin Jiang","doi":"10.1016/j.jcoa.2025.100278","DOIUrl":"10.1016/j.jcoa.2025.100278","url":null,"abstract":"<div><div>The N-terminal region of the polymerase subunit (PAN) is considered as the most promising target for the development of broad-spectrum antivirals, since it is highly conserved in structure and sequences among different influenza strains. In this study, a PAN based at-line nanofractionation (ANF) platform was applied to rapidly screen for PAN inhibitors from <em>Rubus idaeus</em> L. extract which presented good inhibitory effect on PAN with a half maximal inhibitory concentration (IC₅₀) value of 11 μg/mL. Eight PAN inhibitors were successfully screened out, comprising four organic acids and four flavones. In parallel, magnetic ligand fishing was used to isolate the PAN ligands from the extract, and finally four compounds were fished out and considered as PAN ligands (including avicularin, quercetin-3-O-rutinoside, ellagic acid and luteoloside) which were also identified by the ANF platform. Among them, ellagic acid and luteoloside displayed satisfactory in vitro inhibitory activity against PAN, with IC₅₀ values of 1.31 ± 0.33 and 6.23 ± 0.97 μM, respectively. The molecular docking and dynamic simulations revealed that ellagic acid and luteoloside bind to PAN in a distinctly different mode compared to baloxavir. These compounds formed direct interactions with catalytic pocket residues and adjacent amino acids in PAN, establishing hydrogen bonds and water-mediated bridges that modulated the protein's secondary structure and stability. The result from the study suggests that ellagic acid and luteoloside could serve as promising anti-influenza molecules. Furthermore, the ANF platform was proved effective for high-throughput screening of PAN inhibitors from complex herbal extracts, highlighting its significant potential for future anti-influenza drug development.</div></div>","PeriodicalId":93576,"journal":{"name":"Journal of chromatography open","volume":"8 ","pages":"Article 100278"},"PeriodicalIF":3.2,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145473829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From multimodal liquid chromatography to supercritical fluid chromatography: Mapping chiral separation of the major organic ultraviolet filters","authors":"Leandro Oka-Duarte ,&nbsp;Giovanni S. Baviera ,&nbsp;Weston J. Umstead ,&nbsp;Quezia B. Cass ,&nbsp;Anderson R.M. de Oliveira","doi":"10.1016/j.jcoa.2025.100251","DOIUrl":"10.1016/j.jcoa.2025.100251","url":null,"abstract":"<div><div>The widespread presence of chiral organic UV filters in the environment raises critical concerns due to their potential enantioselective toxicity and persistence. This study provides the first comprehensive enantioseparation screening of five such compounds – enzacamene, homosalate, octinoxate, octisalate, and octocrylene – using multimodal liquid chromatography (LC), as well as supercritical fluid chromatography (SFC) with 20 polysaccharide-based chiral stationary phases (CSPs). Enzacamene and homosalate were baseline-resolved across all tested conditions, including SFC, while octisalate showed separation in normal and reversed-phase modes. Octinoxate was resolved only in normal-phase mode, and octocrylene was not fully resolved, though near-baseline separation was achieved in polar organic and normal-phase modes. SFC, particularly with non-conventional CO₂/hexane–ethanol eluent, proved complementary to LC, achieving comparable or superior enantioresolution. Coated amylose columns demonstrated superior performance in reversed-phase mode, whereas immobilized CSPs, particularly chloromethylphenylcarbamate-based selectors, displayed broader applicability across multiple mobile phase systems. These findings emphasize the necessity of multimodal approaches to optimize the enantioseparation of highly lipophilic environmental contaminants and establish a robust analytical foundation for future ecotoxicological and regulatory studies.</div></div>","PeriodicalId":93576,"journal":{"name":"Journal of chromatography open","volume":"8 ","pages":"Article 100251"},"PeriodicalIF":3.2,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145010209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}