Journal of chromatography open最新文献_第9页

Unexplored drug combinations for analytical method development by HPLC 用于 HPLC 分析方法开发的未探索药物组合

Journal of chromatography open Pub Date : 2024-02-22 DOI: 10.1016/j.jcoa.2024.100123

Bibhu Prasad Nanda, Priyanka Rani, Rohit Bhatia

{"title":"Unexplored drug combinations for analytical method development by HPLC","authors":"Bibhu Prasad Nanda, Priyanka Rani, Rohit Bhatia","doi":"10.1016/j.jcoa.2024.100123","DOIUrl":"https://doi.org/10.1016/j.jcoa.2024.100123","url":null,"abstract":"<div><p>The letter talks about opinions in the scientific literature that discuss drug combinations. Specifically, it mentions that these combinations lack analytical methods and are published on the Internet. The development of analytical methods involves systematically creating and improving procedures for techniques such as UV/HPLC or mass spectrometry (MS). This ensures accurate analysis and measurement of chemical compounds in a sample. Improving these methods can optimize production processes, leading to increased efficiency, cost-effectiveness, and environmentally friendly practices. The manuscript presents summarized information gathered from various sources about potential drug combinations lacking online analytical methods. This data highlights the importance of addressing this gap and emphasizes the need for robust analytical methods in exploring and understanding these drug combinations. The letter addressed the significance of advancing analytical methods for drug products and drug analysis holds substantial potential to make a meaningful impact in the medical and biomedical fields.</p></div>","PeriodicalId":93576,"journal":{"name":"Journal of chromatography open","volume":"5 ","pages":"Article 100123"},"PeriodicalIF":0.0,"publicationDate":"2024-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2772391724000100/pdfft?md5=f2b03df2af44b2dc68525b6615ed0992&pid=1-s2.0-S2772391724000100-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139992908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A comparative assessment of low- and high-resolution gas chromatography / mass spectrometry methods for polychlorinated biphenyl congener analysis in industry wastewater 用于工业废水中多氯联苯同系物分析的低分辨率和高分辨率气相色谱/质谱法比较评估

Journal of chromatography open Pub Date : 2024-02-20 DOI: 10.1016/j.jcoa.2024.100124

Waruna Kiridena, Paul Wiegand, Matthew Booth, Cher Lindelien

{"title":"A comparative assessment of low- and high-resolution gas chromatography / mass spectrometry methods for polychlorinated biphenyl congener analysis in industry wastewater","authors":"Waruna Kiridena, Paul Wiegand, Matthew Booth, Cher Lindelien","doi":"10.1016/j.jcoa.2024.100124","DOIUrl":"https://doi.org/10.1016/j.jcoa.2024.100124","url":null,"abstract":"<div><p>A comprehensive study was conducted to understand the performance of USEPA Method 1628 for the testing of Polychlorinated Biphenyl (PCB) Congeners by low-resolution Gas Chromatography-Mass Spectrometry (GCMS–). Method performance was compared to USEPA Method 1668C, USEPA's high-resolution GC–MS method, using nine pulp and paper wastewater samples. USEPA Method 1628 was designed to make PCB analysis more feasible in standard, mid-sized environmental laboratories. This method, which analyzes all PCB congeners, is less sensitive and less expensive to implement than USEPA's high-resolution GC–MS, Method 1668C. The analysis of wastewater samples revealed several potential concerns with Method 1628, including the likelihood of false positive or positively biased identifications of certain congeners. The multi-laboratory validation study conducted by the USEPA indicated that similar problems might have arisen, although a comparison with Method 1668C was not included in that study. The Quality Assurance (QA) aspects of Methods 1628 and 1668C were compared and key issues were identified. Method 1628 can yield results that differ from those obtained with Method 1668C, sometimes quite substantially. Comparison of quantitated results between the two methods could only be applied to those individual analytes that had homologous labeled isotope standards for each method. The percent difference of the reported concentration of the same analyte compared between each method ranged from -16 % to 6241 %. Furthermore, there is a significant risk that Method 1628 may produce false positives and/or positive bias, which could impact the utility of this method if used for some regulatory purposes.</p></div>","PeriodicalId":93576,"journal":{"name":"Journal of chromatography open","volume":"5 ","pages":"Article 100124"},"PeriodicalIF":0.0,"publicationDate":"2024-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2772391724000112/pdfft?md5=5d6deea302dff0715f03c92f5f70a560&pid=1-s2.0-S2772391724000112-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139936976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evolution and applications of Next Generation Sequencing and its intricate relations with chromatographic and spectrometric techniques in modern day sciences 下一代测序技术的发展和应用及其与现代科学中色谱和光谱技术的复杂关系

Journal of chromatography open Pub Date : 2024-02-14 DOI: 10.1016/j.jcoa.2024.100121

Annapurna Katara, Sumit Chand, Harshvardhan Chaudhary, Vijeta Chaudhry, Harish Chandra, Ramesh Chandra Dubey

{"title":"Evolution and applications of Next Generation Sequencing and its intricate relations with chromatographic and spectrometric techniques in modern day sciences","authors":"Annapurna Katara, Sumit Chand, Harshvardhan Chaudhary, Vijeta Chaudhry, Harish Chandra, Ramesh Chandra Dubey","doi":"10.1016/j.jcoa.2024.100121","DOIUrl":"10.1016/j.jcoa.2024.100121","url":null,"abstract":"<div><p>Next generation sequencing (NGS) technology offers new opportunities in biological research. In the last few decades, remarkable technological innovations have emerged, allowing the exploration of unexplored areas of functional genomics and biomedical research at a cost-effective and unprecedented scale and speed. Along with such revolutionary advances came new challenges in methodologies and informatics. Despite not being directly engaged in NGS methods, chromatography's inclusion makes the sequencing process less error prone. These advanced technologies offered novel and rapid ways to sequence static genomes as well as entire transcriptomes for expression analysis under different conditions. Utilization of next-generation sequencing has the potential to entirely rewrite how biological research is conducted and chromatography plays a crucial role in this process. New technologies are evolving rapidly, and addressing prospective issues like the amelioration of protocols for generating sequencing libraries, offering new strategies for data analysis, and most importantly, restructuring and revamping experimental design. In this review, the emergence and evolution of deoxyribonucleic acid (DNA) sequencing techniques from the classical Sanger DNA sequencing method to modern next generation technologies are reviewed, and their applications in-conjugation with chromatographic and spectrometric techniques in modern biological studies are discussed.</p></div>","PeriodicalId":93576,"journal":{"name":"Journal of chromatography open","volume":"5 ","pages":"Article 100121"},"PeriodicalIF":0.0,"publicationDate":"2024-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2772391724000082/pdfft?md5=a4bdc3e8322c1d68ba5511a10c2cafdc&pid=1-s2.0-S2772391724000082-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139814571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Validation of a purity method for a Suzuki-Miyaura boronic ester by liquid chromatography with derivatization 液相色谱法衍生化验证一种铃木-宫拉硼酸酯的纯度方法

Journal of chromatography open Pub Date : 2024-02-05 DOI: 10.1016/j.jcoa.2024.100120

John J. Salisbury , William Georgian , Michael Herr , Michele Buetti-Weekly

{"title":"Validation of a purity method for a Suzuki-Miyaura boronic ester by liquid chromatography with derivatization","authors":"John J. Salisbury , William Georgian , Michael Herr , Michele Buetti-Weekly","doi":"10.1016/j.jcoa.2024.100120","DOIUrl":"https://doi.org/10.1016/j.jcoa.2024.100120","url":null,"abstract":"<div><p>The Miyaura borylation reaction enables the direct synthesis of boronates, a valuable partner in Suzuki-Miyaura coupling reactions. These C-C coupling reactions provide one of the most efficient methods for the construction of C-C bonds and are widely used in transition metal-catalyzed organic chemistry. However, analyses of these boronic acid/ester products and their derivatives are rarely discussed. In this communication, a sensitive and selective liquid chromatography method has been validated for the purity determination of a boronic ester intermediate where significant sample preparation challenges were observed. An efficient <em>in-situ</em> derivatization was implemented which consisted of a hydrolysis to the boronic acid derivative while simultaneously poisoning any residual active palladium. The HPLC analysis was performed on an Zorbax XDB-C18 (150 mm x 4.6 mm) 3.5 µm column utilizing water/acetonitrile gradient elution with an acidic modifier of 0.5 % trifluoroacetic acid at a flow rate of 1.0 mL/min. The detection was performed at the wavelength of 254 nm, and the retention time of the boronic ester was around 4.9 min. The proposed method was validated to ICH Q2 standards and included such parameters as specificity, system precision, accuracy/reproducibility, linearity, LOD/LOQ and solution stability.</p></div>","PeriodicalId":93576,"journal":{"name":"Journal of chromatography open","volume":"5 ","pages":"Article 100120"},"PeriodicalIF":0.0,"publicationDate":"2024-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2772391724000070/pdfft?md5=dab7a64a810126665afaaf73d7c72fd0&pid=1-s2.0-S2772391724000070-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139714274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Semi-Automated Pipeline for Nontargeted Compound Analysis via GC×GC-MS 通过 GC×GC-MS 进行非靶向化合物分析的半自动化管道

Journal of chromatography open Pub Date : 2024-02-05 DOI: 10.1016/j.jcoa.2024.100118

Daniel R. Struk , Rizky Ilhamsyah , Christopher A. Heist , Jean-Marie D. Dimandja , Peter J. Hesketh

引用次数: 0

Robust polydimethylsiloxane-polydopamine coated stir bar for rapid extraction of malachite green, crystal violet and their metabolites in aquatic waters and determined by portable mass spectrometry 坚固耐用的聚二甲基硅氧烷-聚多巴胺涂层搅拌棒，用于快速萃取水体中的孔雀石绿、结晶紫及其代谢物，并通过便携式质谱仪进行测定

Journal of chromatography open Pub Date : 2024-02-02 DOI: 10.1016/j.jcoa.2024.100119

Jinya Cao , Yuxia Shen , Zhaixin Liu , Shanshan Zhu , Huihui Wu , Yali Dang , Tianhua Li , Ning Gan

{"title":"Robust polydimethylsiloxane-polydopamine coated stir bar for rapid extraction of malachite green, crystal violet and their metabolites in aquatic waters and determined by portable mass spectrometry","authors":"Jinya Cao , Yuxia Shen , Zhaixin Liu , Shanshan Zhu , Huihui Wu , Yali Dang , Tianhua Li , Ning Gan","doi":"10.1016/j.jcoa.2024.100119","DOIUrl":"https://doi.org/10.1016/j.jcoa.2024.100119","url":null,"abstract":"<div><p>Triphenylmethane (TPM) fish drug residues and their metabolites exist in many aquiculture waters and can cause significant harm to consumers. Rapid, accurate, and simultaneous detection of their residues in foods is significant in protecting the safety of aquatic products. In the study, a new type of polydopamine coated polydimethylsiloxane (PDMS-PDA) stir bar was prepared for the automatic sorptive extraction and enrichment of malachite green, leucomalachite green, crystal violet, and leucocrystal violet which are common TPM drugs used in aquaculture. The surface of PDMS matrix was first turned to hydrophilic by plasma treatment to enhance the evenness and stability of PDA coating. Therefore, the PDMS-PDA stir bar can enhance its extraction capacity and selectivity towards TPMs. These were due to the synergisitic adsorption capacities of PDA and PDMS towards the dyes. A hand-held stirring device was installed with the stir bar for on-site extraction. After that, a portable mass spectrometer directly detected the eluted targets without chromatography separation. Under the optimized conditions. The four analytes' lower detection limit of 0.16–0.38 μg L<sup>-1</sup> was obtained. Within 35 min, the stir bar device, combing with a portable mass spectrometer, was used for rapid pretreatment and real sample analysis of four analytes with a recovery of 84.3–110 %. All these prove that the assay exhibited potential application prospects for the on-site detection of TPM residues in aquatic waters.</p></div>","PeriodicalId":93576,"journal":{"name":"Journal of chromatography open","volume":"5 ","pages":"Article 100119"},"PeriodicalIF":0.0,"publicationDate":"2024-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2772391724000069/pdfft?md5=4008feea6900ece7d22771f8e086a908&pid=1-s2.0-S2772391724000069-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139714056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A method to calculate 2nd dimension compound retention index in GC×GC system based on reference n-alkane and aromatic hydrocarbon mixture 基于参考正烷烃和芳香烃混合物计算 GC×GC 系统中二维化合物保留指数的方法

Journal of chromatography open Pub Date : 2024-02-01 DOI: 10.1016/j.jcoa.2024.100117

Rizky Ilhamsyah , Daniel R. Struk , Christopher A. Heist , Peter J. Hesketh , Jean-Marie D. Dimandja

引用次数: 0

Evolution and applications of Next Generation Sequencing and its intricate relations with chromatographic and spectrometric techniques in modern day sciences 下一代测序技术的发展和应用及其与现代科学中色谱和光谱技术的复杂关系

Journal of chromatography open Pub Date : 2024-02-01 DOI: 10.1016/j.jcoa.2024.100121

Annapurna Katara, Sumit Chand, Harshvardhan Chaudhary, Vijeta Chaudhry, Harish Chandra, Ramesh Chandra Dubey

引用次数: 0

Identification of coronavirus disease marker compounds in sweat with comprehensive two dimensional gas chromatography using multiloop splitter-based non-cryogenic artificial trapping modulation system 利用基于多回路分流器的非致冷人工捕获调制系统的综合二维气相色谱法鉴定汗液中的冠状病毒疾病标记化合物

Journal of chromatography open Pub Date : 2024-01-10 DOI: 10.1016/j.jcoa.2023.100113

Nuttanee Tungkijanansin , Ryland T. Giebelhaus , Sheri A. Schmidt , Thumnoon Nhujak , Kaywalee Chatdarong , Pattama Torvorapanit , James J. Harynuk , Chadin Kulsing

{"title":"Identification of coronavirus disease marker compounds in sweat with comprehensive two dimensional gas chromatography using multiloop splitter-based non-cryogenic artificial trapping modulation system","authors":"Nuttanee Tungkijanansin , Ryland T. Giebelhaus , Sheri A. Schmidt , Thumnoon Nhujak , Kaywalee Chatdarong , Pattama Torvorapanit , James J. Harynuk , Chadin Kulsing","doi":"10.1016/j.jcoa.2023.100113","DOIUrl":"10.1016/j.jcoa.2023.100113","url":null,"abstract":"<div><p>This study applied comprehensive two-dimensional gas chromatography-mass spectrometry (GC × GCMS–) for analysis of volatile compounds in headspaces of axillary swab samples of RT-PCR or antigen test kit proven COVID-19 positive and negative volunteers in Bangkok, Thailand. The separation was performed using a conventional Deans switch (DS) based heartcut system with an additional splitter system located between the first (<sup>1</sup>D) column (30 m) and the DS. The splitter consisted of deactivated fused silica (DFS) columns connected into three loops with the progressively doubled perimeters of the downstream loops. An artificial GC × GC result was obtained by applying a periodic heartcut (H/C) event within every artificial modulation period (<em>P</em><sub>AM</sub>) of 1.14 min, selectively transferring the analytes onto the second (<sup>2</sup>D) column (30 m). Chemometric analysis including feature selection was used to identify significantly altered metabolites between the positive and negative groups. Fourteen significant metabolites were tentatively identified, including <em>p</em>-cymene, linalool, and 2,6,11-trimethyldodecane. The marker peak area thresholds were optimized by generating receiver operating characteristic (ROC) curves showing accuracy, sensitivity and selectivity within the ranges of 94–98%, 93–97% and 94–100%, respectively. Based on these results, we hypothesize that SARS-CoV-2 infection disrupts the metabolism of volatile metabolites in sweat, or impacts the microbiome, changing the volatile profile of sweat in infected patients.</p></div>","PeriodicalId":93576,"journal":{"name":"Journal of chromatography open","volume":"5 ","pages":"Article 100113"},"PeriodicalIF":0.0,"publicationDate":"2024-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2772391723000373/pdfft?md5=0f1eb48265a7df79af756c20ce95c4f3&pid=1-s2.0-S2772391723000373-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139457804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Erratum to “Optimization of multi flow rate loading strategy for process intensification of Protein A chromatography” Journal of Chromatography Open, Volume 2, November 2022, 100049 蛋白质 A 色谱过程强化的多流速加载策略优化》勘误，《色谱开放杂志》，第 2 卷，2022 年 11 月，100049 页

Journal of chromatography open Pub Date : 2024-01-09 DOI: 10.1016/j.jcoa.2024.100114

Anjali Ramakrishna , Vijay Maranholkar , Sandeep Hadpe , Jyoti Iyer , Anurag Rathore

引用次数: 0