Capillary electrochromatography applied to the separation of enantiomers utilizing packed capillary columns with silica-vancomycin. A tutorial

Abstract

The separation and analysis of chiral compounds is an important topic in various fields such as research, pharmaceutical industry, food control, agrochemistry, biochemistry, forensics and toxicology, etc. The interest in the mentioned fields is mainly due to the fact that enantiomers could react with the chiral environment. Therefore, their separation and analysis are a challenging issue because the use of these compounds can be related to humans and animals’ lives as well as to the environment (e.g., soil and water). Their separation can be performed with different analytical techniques such as high-performance liquid chromatography, gas chromatography, supercritical fluid chromatography, and microfluidic technique (capillary electrophoresis and capillary electrochromatography-CEC). Usually the direct resolution method is applied making use of a chiral stationary phase (CSP). This tutorial is aimed at illustrating the main features of CEC using a packed capillary, e.g., employing a silica-vancomycin CSP. The synthesis of the CSP, the instrumentation of CEC coupled with UV and mass spectrometry detectors, method optimization, and two selected applications are presented and discussed.