PhotoniX最新文献_第2页

Three-dimensional dipole orientation mapping with high temporal-spatial resolution using polarization modulation 利用偏振调制实现高时空分辨率的三维偶极子方位制图

PhotoniX Pub Date : 2024-04-16 DOI: 10.1186/s43074-024-00127-6

Suyi Zhong, Liang Qiao, Xichuan Ge, Xinzhu Xu, Yuzhe Fu, Shu Gao, Karl Zhanghao, Huiwen Hao, Wenyi Wang, Meiqi Li, Peng Xi

{"title":"Three-dimensional dipole orientation mapping with high temporal-spatial resolution using polarization modulation","authors":"Suyi Zhong, Liang Qiao, Xichuan Ge, Xinzhu Xu, Yuzhe Fu, Shu Gao, Karl Zhanghao, Huiwen Hao, Wenyi Wang, Meiqi Li, Peng Xi","doi":"10.1186/s43074-024-00127-6","DOIUrl":"https://doi.org/10.1186/s43074-024-00127-6","url":null,"abstract":"Fluorescence polarization microscopy is widely used in biology for molecular orientation properties. However, due to the limited temporal resolution of single-molecule orientation localization microscopy and the limited orientation dimension of polarization modulation techniques, achieving simultaneous high temporal-spatial resolution mapping of the three-dimensional (3D) orientation of fluorescent dipoles remains an outstanding problem. Here, we present a super-resolution 3D orientation mapping (3DOM) microscope that resolves 3D orientation by extracting phase information of the six polarization modulation components in reciprocal space. 3DOM achieves an azimuthal precision of 2° and a polar precision of 3° with spatial resolution of up to 128 nm in the experiments. We validate that 3DOM not only reveals the heterogeneity of the milk fat globule membrane, but also elucidates the 3D structure of biological filaments, including the 3D spatial conformation of λ-DNA and the structural disorder of actin filaments. Furthermore, 3DOM images the dipole dynamics of microtubules labeled with green fluorescent protein in live U2OS cells, reporting dynamic 3D orientation variations. Given its easy integration into existing wide-field microscopes, we expect the 3DOM microscope to provide a multi-view versatile strategy for investigating molecular structure and dynamics in biological macromolecules across multiple spatial and temporal scales.","PeriodicalId":93483,"journal":{"name":"PhotoniX","volume":"68 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140603412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multiplexing near- and far-field functionalities with high-efficiency bi-channel metasurfaces 利用高效双通道元表面复用近场和远场功能

PhotoniX Pub Date : 2024-04-15 DOI: 10.1186/s43074-024-00128-5

Changhong Dai, Tong Liu, Dongyi Wang, Lei Zhou

{"title":"Multiplexing near- and far-field functionalities with high-efficiency bi-channel metasurfaces","authors":"Changhong Dai, Tong Liu, Dongyi Wang, Lei Zhou","doi":"10.1186/s43074-024-00128-5","DOIUrl":"https://doi.org/10.1186/s43074-024-00128-5","url":null,"abstract":"Propagating waves and surface waves are two distinct types of light-transporting modes, the free control of which are both highly desired in integration photonics. However, previously realized devices are bulky in sizes, inefficient, and/or can only achieve one type of light-manipulation functionality with a single device. Here, we propose a generic approach to design bi-channel meta-devices, constructed by carefully selected meta-atoms possessing reflection phases of both structural-resonance and geometric origins, which can exhibit two distinct light-manipulation functionalities in near-field (NF) and far-field (FF) channels, respectively. After characterizing the scattering properties of basic meta-atoms and briefly stating the theoretical strategy, we design/fabricate three different meta-devices and experimentally characterize their bi-channel wave-control functionalities in the telecom regime. Our experiments show that the first two devices can multiplex the generations of NF and FF optical vortices with different topological charges, while the third one exhibits anomalous surface plasmon polariton focusing in the NF and hologram formation in the FF simultaneously. Our results expand the wave-control functionalities of metasurfaces to all wave-transporting channels, which may inspire many exciting applications in integration optics.","PeriodicalId":93483,"journal":{"name":"PhotoniX","volume":"9 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140586593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ultra-wideband terahertz fingerprint enhancement sensing and inversion model supported by single-pixel reconfigurable graphene metasurface 单像素可重构石墨烯元表面支持的超宽带太赫兹指纹增强传感和反转模型

PhotoniX Pub Date : 2024-04-15 DOI: 10.1186/s43074-024-00129-4

Bingwei Liu, Yan Peng, YuFan Hao, Yiming Zhu, Shengjiang Chang, Songlin Zhuang

{"title":"Ultra-wideband terahertz fingerprint enhancement sensing and inversion model supported by single-pixel reconfigurable graphene metasurface","authors":"Bingwei Liu, Yan Peng, YuFan Hao, Yiming Zhu, Shengjiang Chang, Songlin Zhuang","doi":"10.1186/s43074-024-00129-4","DOIUrl":"https://doi.org/10.1186/s43074-024-00129-4","url":null,"abstract":"The molecular fingerprint sensing technology based on metasurface has unique attraction in the biomedical field. However, in the terahertz (THz) band, existing metasurface designs based on multi-pixel or angle multiplexing usually require more analyte amount or possess a narrower tuning bandwidth. Here, we propose a novel single-pixel graphene metasurface. Based on the synchronous voltage tuning, this metasurface enables ultra-wideband ((sim) 1.5 THz) fingerprint enhancement sensing of trace analytes, including chiral optical isomers, with a limit of detection (LoD) ≤ 0.64 μg/mm2. The enhancement of the fingerprint signal ((sim) 17.4 dB) originates from the electromagnetically induced transparency (EIT) effect excited by the metasurface, and the ideal overlap between the light field constrained by single-layer graphene (SLG) and ultra-thin analyte. Meanwhile, due to the unique nonlinear enhancement mechanism in graphene tuning, the absorption envelope distortion is inevitable. To solve this problem, a universal fingerprint spectrum inversion model is developed for the first time, and the restoration of standard fingerprints reaches Rmax2 ≥ 0.99. In addition, the asynchronous voltage tuning of the metasurface provides an opportunity for realizing the dynamic reconfiguration of EIT resonance and the slow light modulation in the broadband range. This work builds a bridge for ultra-wideband THz fingerprint sensing of trace analytes, and has potential applications in active spatial light modulators, slow light devices and dynamic imaging equipments.","PeriodicalId":93483,"journal":{"name":"PhotoniX","volume":"6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140582007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Brillouin microscopy monitors rapid responses in subcellular compartments 布里渊显微镜监测亚细胞区的快速反应

PhotoniX Pub Date : 2024-04-10 DOI: 10.1186/s43074-024-00123-w

Zachary N. Coker, Maria Troyanova-Wood, Zachary A. Steelman, Bennett L. Ibey, Joel N. Bixler, Marlan O. Scully, Vladislav V. Yakovlev

{"title":"Brillouin microscopy monitors rapid responses in subcellular compartments","authors":"Zachary N. Coker, Maria Troyanova-Wood, Zachary A. Steelman, Bennett L. Ibey, Joel N. Bixler, Marlan O. Scully, Vladislav V. Yakovlev","doi":"10.1186/s43074-024-00123-w","DOIUrl":"https://doi.org/10.1186/s43074-024-00123-w","url":null,"abstract":"Measurements and imaging of the mechanical response of biological cells are critical for understanding the mechanisms of many diseases, and for fundamental studies of energy, signal and force transduction. The recent emergence of Brillouin microscopy as a powerful non-contact, label-free way to non-invasively and non-destructively assess local viscoelastic properties provides an opportunity to expand the scope of biomechanical research to the sub-cellular level. Brillouin spectroscopy has recently been validated through static measurements of cell viscoelastic properties, however, fast (sub-second) measurements of sub-cellular cytomechanical changes have yet to be reported. In this report, we utilize a custom multimodal spectroscopy system to monitor for the very first time the rapid viscoelastic response of cells and subcellular structures to a short-duration electrical impulse. The cytomechanical response of three subcellular structures - cytoplasm, nucleoplasm, and nucleoli - were monitored, showing distinct mechanical changes despite an identical stimulus. Through this pioneering transformative study, we demonstrate the capability of Brillouin spectroscopy to measure rapid, real-time biomechanical changes within distinct subcellular compartments. Our results support the promising future of Brillouin spectroscopy within the broad scope of cellular biomechanics.","PeriodicalId":93483,"journal":{"name":"PhotoniX","volume":"27 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140586592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Three-channel robust optical encryption via engineering coherence Stokes vector of partially coherent light 通过部分相干光的工程相干斯托克斯矢量实现三信道稳健光学加密

PhotoniX Pub Date : 2024-04-03 DOI: 10.1186/s43074-024-00126-7

Yonglei Liu, Zhen Dong, Yimeng Zhu, Haiyun Wang, Fei Wang, Yahong Chen, Yangjian Cai

引用次数: 0

Visualizing cortical blood perfusion after photothrombotic stroke in vivo by needle-shaped beam optical coherence tomography angiography 通过针形光束光学相干断层血管成像技术观察光血栓性中风后体内皮层血液灌注情况

PhotoniX Pub Date : 2024-03-27 DOI: 10.1186/s43074-024-00124-9

Xiangyu Guo, Jingjing Zhao, Liqun Sun, Varun Gupta, Lin Du, Komal Sharma, Aidan Van Vleck, Kaitlyn Liang, Liangcai Cao, Lingjie Kong, Yuanmu Yang, Yong Huang, Adam de la Zerda, Guofan Jin

{"title":"Visualizing cortical blood perfusion after photothrombotic stroke in vivo by needle-shaped beam optical coherence tomography angiography","authors":"Xiangyu Guo, Jingjing Zhao, Liqun Sun, Varun Gupta, Lin Du, Komal Sharma, Aidan Van Vleck, Kaitlyn Liang, Liangcai Cao, Lingjie Kong, Yuanmu Yang, Yong Huang, Adam de la Zerda, Guofan Jin","doi":"10.1186/s43074-024-00124-9","DOIUrl":"https://doi.org/10.1186/s43074-024-00124-9","url":null,"abstract":"Optical imaging techniques provide low-cost, non-radiative images with high spatiotemporal resolution, making them advantageous for long-term dynamic observation of blood perfusion in stroke research and other brain studies compared to non-optical methods. However, high-resolution imaging in optical microscopy fundamentally requires a tight optical focus, and thus a limited depth of field (DOF). Consequently, large-scale, non-stitched, high-resolution images of curved surfaces, like brains, are difficult to acquire without z-axis scanning. To overcome this limitation, we developed a needle-shaped beam optical coherence tomography angiography (NB-OCTA) system, and for the first time, achieved a volumetric resolution of less than 8 μm in a non-stitched volume space of 6.4 mm × 4 mm × 620 μm in vivo. This system captures the distribution of blood vessels at 3.4-times larger depths than normal OCTA equipped with a Gaussian beam (GB-OCTA). We then employed NB-OCTA to perform long-term observation of cortical blood perfusion after stroke in vivo, and quantitatively analyzed the vessel area density (VAD) and the diameters of representative vessels in different regions over 10 days, revealing different spatiotemporal dynamics in the acute, sub-acute and chronic phase of post-ischemic revascularization. Benefiting from our NB-OCTA, we revealed that the recovery process is not only the result of spontaneous reperfusion, but also the formation of new vessels. This study provides visual and mechanistic insights into strokes and helps to deepen our understanding of the spontaneous response of brain after stroke.","PeriodicalId":93483,"journal":{"name":"PhotoniX","volume":"235 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140314275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ultrafast laser one-step construction of 3D micro-/nanostructures achieving high-performance zinc metal anodes 超快激光一步法构建三维微/纳米结构，实现高性能锌金属阳极

PhotoniX Pub Date : 2024-03-19 DOI: 10.1186/s43074-024-00122-x

Yanan Liu, Ye Ding, Zeping Liu, Xingchen Li, Sichao Tian, Lishuang Fan, Jichang Xie, Liangliang Xu, Jinwoo Lee, Jian Li, Lijun Yang

{"title":"Ultrafast laser one-step construction of 3D micro-/nanostructures achieving high-performance zinc metal anodes","authors":"Yanan Liu, Ye Ding, Zeping Liu, Xingchen Li, Sichao Tian, Lishuang Fan, Jichang Xie, Liangliang Xu, Jinwoo Lee, Jian Li, Lijun Yang","doi":"10.1186/s43074-024-00122-x","DOIUrl":"https://doi.org/10.1186/s43074-024-00122-x","url":null,"abstract":"Aqueous zinc-ion batteries provide a most promising alternative to the existing lithium-ion batteries due to their high theoretical capacity, intrinsic safety, and low cost. However, commercializing aqueous zinc-ion batteries suffer from dendritic growth and side reactions on the surface of metallic zinc, resulting in poor reversibility. To overcome this critical challenge, here, we report a one-step ultrafast laser processing method for fabricating three-dimensional micro-/nanostructures on zinc anodes to optimize zinc nucleation and deposition processes. It is demonstrated that the three-dimensional micro-/nanostructure with increased specific surface area significantly reduces nucleation overpotential, as well as preferentially absorbs zinc ions to prevent dendritic protuberances and corrosion. As a result, the presence of three-dimensional micro-/nanostructures on the zinc metal delivers stable zinc plating/stripping beyond 2500 h (2 mA cm-2/1 mAh cm-2) in symmetric cells, a high Coulombic efficiency (99.71%) in half cells, and moreover an improved capacity retention (71.8%) is also observed in full cells. Equally intriguingly, the pouch cell with three-dimensional micro-/nanostructures can operate across various bending states without severely compromising performance. This work provides an effective strategy to construct ultrafine and high-precision three-dimensional micro-/nanostructures achieving high-performance zinc metal anodes and is expected to be of immediate benefit to other metal-based electrodes.","PeriodicalId":93483,"journal":{"name":"PhotoniX","volume":"27 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140171802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Vacuum-ultraviolet (λ < 200 nm) photodetector array 真空-紫外线（λ < 200 纳米）光电探测器阵列

PhotoniX Pub Date : 2024-03-13 DOI: 10.1186/s43074-024-00120-z

Siqi Zhu, Zhuogeng Lin, Zhao Wang, Lemin Jia, Naiji Zhang, Wei Zheng

引用次数: 0

Self-supervised denoising for multimodal structured illumination microscopy enables long-term super-resolution live-cell imaging 用于多模态结构照明显微镜的自监督去噪技术可实现长期超分辨率活细胞成像

PhotoniX Pub Date : 2024-03-01 DOI: 10.1186/s43074-024-00121-y

Xingye Chen, Chang Qiao, Tao Jiang, Jiahao Liu, Quan Meng, Yunmin Zeng, Haoyu Chen, Hui Qiao, Dong Li, Jiamin Wu

{"title":"Self-supervised denoising for multimodal structured illumination microscopy enables long-term super-resolution live-cell imaging","authors":"Xingye Chen, Chang Qiao, Tao Jiang, Jiahao Liu, Quan Meng, Yunmin Zeng, Haoyu Chen, Hui Qiao, Dong Li, Jiamin Wu","doi":"10.1186/s43074-024-00121-y","DOIUrl":"https://doi.org/10.1186/s43074-024-00121-y","url":null,"abstract":"Detection noise significantly degrades the quality of structured illumination microscopy (SIM) images, especially under low-light conditions. Although supervised learning based denoising methods have shown prominent advances in eliminating the noise-induced artifacts, the requirement of a large amount of high-quality training data severely limits their applications. Here we developed a pixel-realignment-based self-supervised denoising framework for SIM (PRS-SIM) that trains an SIM image denoiser with only noisy data and substantially removes the reconstruction artifacts. We demonstrated that PRS-SIM generates artifact-free images with 20-fold less fluorescence than ordinary imaging conditions while achieving comparable super-resolution capability to the ground truth (GT). Moreover, we developed an easy-to-use plugin that enables both training and implementation of PRS-SIM for multimodal SIM platforms including 2D/3D and linear/nonlinear SIM. With PRS-SIM, we achieved long-term super-resolution live-cell imaging of various vulnerable bioprocesses, revealing the clustered distribution of Clathrin-coated pits and detailed interaction dynamics of multiple organelles and the cytoskeleton.","PeriodicalId":93483,"journal":{"name":"PhotoniX","volume":"24 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140008142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Observation of single-molecule Raman spectroscopy enabled by synergic electromagnetic and chemical enhancement 利用电磁和化学协同增强技术观测单分子拉曼光谱

PhotoniX Pub Date : 2024-02-29 DOI: 10.1186/s43074-024-00119-6

Haiyao Yang, Haoran Mo, Jianzhi Zhang, Lihong Hong, Zhi-Yuan Li

{"title":"Observation of single-molecule Raman spectroscopy enabled by synergic electromagnetic and chemical enhancement","authors":"Haiyao Yang, Haoran Mo, Jianzhi Zhang, Lihong Hong, Zhi-Yuan Li","doi":"10.1186/s43074-024-00119-6","DOIUrl":"https://doi.org/10.1186/s43074-024-00119-6","url":null,"abstract":"There has been a long fundamental pursuit to enhance and levitate the Raman scattering signal intensity of molecule by a huge number of ~ 14–15 orders of magnitude, to the level comparable with the molecule fluorescence intensity and truly entering the regime of single-molecule Raman spectroscopy. In this work we report unambiguous observation of single-molecule Raman spectroscopy via synergic action of electromagnetic and chemical enhancement for rhodamine B (RhB) molecule absorbed within the plasmonic nanogap formed by gold nanoparticle sitting on the two-dimensional (2D) monolayer WS2 and 2 nm SiO2 coated gold thin film. Raman spectroscopy down to an extremely dilute value of 10–18 mol/L can still be clearly visible, and the statistical enhancement factor could reach 16 orders of magnitude compared with the reference detection sample of silicon plate. The electromagnetic enhancement comes from local surface plasmon resonance induced at the nanogap, which could reach ~ 10–11 orders of magnitude, while the chemical enhancement comes from monolayer WS2 2D material, which could reach 4–5 orders of magnitudes. This synergic route of Raman enhancement devices could open up a new frontier of single molecule science, allowing detection, identification, and monitor of single molecules and their spatial–temporal evolution under various internal and external stimuli.","PeriodicalId":93483,"journal":{"name":"PhotoniX","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140008141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0