Kelly A. Meiklejohn , Mary K. Burnham-Curtis , Dyan J. Straughan , Jenny Giles , M. Katherine Moore
{"title":"Current methods, future directions and considerations of DNA-based taxonomic identification in wildlife forensics","authors":"Kelly A. Meiklejohn , Mary K. Burnham-Curtis , Dyan J. Straughan , Jenny Giles , M. Katherine Moore","doi":"10.1016/j.fsiae.2021.100030","DOIUrl":"10.1016/j.fsiae.2021.100030","url":null,"abstract":"<div><p>Wildlife forensic analyses are frequently concerned with taxonomic identification, and very often employ amplification and Sanger sequencing of informative regions of the genome to achieve this. The materials submitted to wildlife forensic laboratories for taxonomic identification span a wide scope, from plant and animal parts in trade to assemblages of incidental biota at crime scenes. As these analyses take place within the context of legal proceedings, the wildlife forensic community is subject to unique requirements and considerations. These requirements and considerations are quite different from those of human forensic DNA, and have driven standardization in this field. While there has been extensive debate over appropriate DNA-based methods for taxonomic identification of a wide variety of biota in research settings, there has been little discussion on the issues associated with this approach in the high scrutiny environment of forensic science. This review outlines: key procedural and biological factors that may impact the accuracy of interpretation and reporting taxonomic identifications; resulting conventions employed by the wildlife forensics community; and implications for the use of emergent DNA sequencing technologies in taxonomic identification of wildlife in casework.</p></div>","PeriodicalId":93435,"journal":{"name":"Forensic science international. Animals and environments","volume":"1 ","pages":"Article 100030"},"PeriodicalIF":0.0,"publicationDate":"2021-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666937421000299/pdfft?md5=2862fbc5c013379ab52262b9cc29825b&pid=1-s2.0-S2666937421000299-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45194047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Brian C. Hamlin , Erin P. Meredith , Jeff Rodzen , Jolene M. Strand
{"title":"OdoPlex: An STR multiplex panel optimized and validated for forensic identification and sex determination of North American mule deer (Odocoileus hemionus) and white-tailed deer (Odocoileus virginianus)","authors":"Brian C. Hamlin , Erin P. Meredith , Jeff Rodzen , Jolene M. Strand","doi":"10.1016/j.fsiae.2021.100026","DOIUrl":"10.1016/j.fsiae.2021.100026","url":null,"abstract":"<div><p>OdoPlex is a single 5-dye multiplex panel for forensic identification of North American mule deer (<em>Odocoileus hemionus</em>) and white-tailed deer (<em>Odocoileus virginianus</em>). This robust panel is comprised of 15 autosomal markers (14 tetranucleotide loci and a single dinucleotide locus) and two sex-linked markers. OdoPlex was optimized and validated in response to the USA-based Organization of Scientific Area Committees (OSAC) research priority calling for the development and validation of standardized STR panels and associated allelic ladders for North American species routinely encountered in wildlife forensic casework. Genotypes were obtained from a combined 1143 white-tailed deer and mule deer representing 18 subspecies, including both the Key deer and Columbian white-tailed deer, two ESA-listed subspecies of white-tailed deer of special interest to population managers. Complete STR genotypes, including sex identification, were generated from as little as 125 pg of DNA template. Methods for species-level assignment and the calculation of single-source likelihood ratios accounting for population substructure are discussed in detail. Median likelihood ratios ranged from 1.65 × 10<sup>10</sup> (<em>O. hemionus</em>) to 1.58 × 10<sup>14</sup> (<em>O. virginianus</em>), making OdoPlex a powerful assay for individual identification of deer in the genus <em>Odocoileus</em>.</p></div>","PeriodicalId":93435,"journal":{"name":"Forensic science international. Animals and environments","volume":"1 ","pages":"Article 100026"},"PeriodicalIF":0.0,"publicationDate":"2021-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.fsiae.2021.100026","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47402281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ashley M. Spicer , Steven A. Lucero , Mark J. McLellan , Lauren E. Goodmiller , Kelly L. Carrothers
{"title":"Determination of the volumetric percentage of ivory content using 3D modeling: Assessment of composite objects and threshold exemption status","authors":"Ashley M. Spicer , Steven A. Lucero , Mark J. McLellan , Lauren E. Goodmiller , Kelly L. Carrothers","doi":"10.1016/j.fsiae.2021.100016","DOIUrl":"10.1016/j.fsiae.2021.100016","url":null,"abstract":"<div><p>Measurement of the percentage volume of the ivory constituent of a composite object is necessary to ensure compliance with the ivory volume provision in California Fish and Game Code (FGC) section 2022. Assessment of the ivory percentage must be conducted as a percentage of the total volume of the composite object and be of a non-destructive nature. To meet these analytical requirements, optical three-dimensional (3D) scanning and imaging software algorithms were used to create 3D models of three composite objects and to measure the volumetric percentage of the ivory constituents. Volumetric percentage determination of the ivory constituents was conducted on the composite objects in both the assembled and the disassembled states to assess the percentage error (PE) for ivory volume measurements when destruction of an item is not permitted. For objects or carvings with clearly defined boundary lines, a PE of <5% is recommended to be added to the volumetric ivory findings to factor in the presence of hidden, non-visible ivory. For an object without clearly defined boundary lines between ivory constituents and adjacent non-ivory constituents, the PE was dependent on the size, type, and number of ivory interfaces. The validity of the optical 3D scanner was assessed using a reference object of known geometry and shown to be accurate within a PE of ±0.3%. The findings of this study suggest that the volumetric percentage of ivory for a composite object can be accurately determined using a non-destructive optical 3D scanning method.</p></div>","PeriodicalId":93435,"journal":{"name":"Forensic science international. Animals and environments","volume":"1 ","pages":"Article 100016"},"PeriodicalIF":0.0,"publicationDate":"2021-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.fsiae.2021.100016","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"110204378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Erin R. Price , Isabella Miles-Bunch , Peter E. Gasson , Cady A. Lancaster
{"title":"Inference of origin of Pterocarpus timber by chemical profiling of ambient ionization mass spectra","authors":"Erin R. Price , Isabella Miles-Bunch , Peter E. Gasson , Cady A. Lancaster","doi":"10.1016/j.fsiae.2021.100032","DOIUrl":"10.1016/j.fsiae.2021.100032","url":null,"abstract":"<div><p>In <em>Pterocarpus</em>, three out of 46 tree species (<em>P. erinaceus</em>, <em>P. santalinus</em>, and <em>P. tinctorius</em>) are listed as endangered and protected under Appendix II of CITES, and the status of several <em>Pterocarpus</em> species suggests that more will find a place on CITES in the future. As unsustainable forest exploitation has increased, regulations for timber traceability have also increased with the creation of such laws as the 2005 Lacey Act in the United States (Lacey Act, 2005) and the European Union Timber Regulation (EUTR) in 2013. These laws were implemented to ensure that members of the timber trade operated in accordance with guidelines and practiced due diligence, with the goal of halting illegal timber imports (FAO, 2016). Unfortunately, the illegal timber trade remains prevalent and has progressed into the third largest transnational crime (Mavrellis, 2017). To combat the prevalence of illegal timber, researchers have utilized Direct Analysis in Real Time Time-of-Flight Mass Spectrometry (DART-TOFMS). With the success found using DART-TOFMS for species identification, we investigated whether the method could be used to identify the original geographic region of timber through analysis of 211 samples taken from 12 <em>Pterocarpus</em> species taken from the Neotropics, Africa, South Asia, and Southeast Asia. We found that analysis using DART-TOFMS could identify the broad geographical provenances of the timber with an accuracy of 98%. This research demonstrated that DART-TOFMS can be used to detect the phytochemical trends that exist between timber species occupying broad geographic regions.</p></div>","PeriodicalId":93435,"journal":{"name":"Forensic science international. Animals and environments","volume":"1 ","pages":"Article 100032"},"PeriodicalIF":0.0,"publicationDate":"2021-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666937421000317/pdfft?md5=0674aeb717be67a998d27dd93f2f074f&pid=1-s2.0-S2666937421000317-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44517677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Identification of Gangetic turtles based on species-specific variations on mitochondrial cyt b and nuclear Cmos genes","authors":"Prabhaker Yadav , Ajit Kumar , Subhashree Sahoo, Neha Yadav, Syed Ainul Hussain, Sandeep Kumar Gupta","doi":"10.1016/j.fsiae.2021.100035","DOIUrl":"10.1016/j.fsiae.2021.100035","url":null,"abstract":"<div><p>The freshwater turtles of the iconic Ganga river system are one such group of vulnerable turtles. Despite common knowledge of the severity of turtle trade in the region, Gangetic turtles continue being poached in large volume, evident from the numerous and extensive seizures across the Gangetic belt. The intensive wildlife trade in Gangetic turtles warrants immediate conservation and management attention. The genetic resource is a vital forensic tool to monitor the Gangetic turtle species to understand the pattern of illegal wildlife trade. We collected 64 softshell and hardshell turtles samples from the Ganga river and report species-specific variations among turtle species based on mitochondrial cytochrome <em>b</em> gene (1140 bp) and nuclear Cmos gene (602 bp). This genetic information will help augment the molecular database to identify Gangetic turtle species and lineages effectively. We identified unique species-specific variable sites, haplotypes, and Single Nucleotide Polymorphisms (SNPs) and analyzed genetic differentiation and phylogenetic relationships. The unique mitochondrial and nuclear signatures exhibited in this study will add to baseline information on the genetic relationship of turtles of river Ganga. It will be helpful in wildlife forensics characterization of the endangered turtles. It will also help in formulating <em>in-situ</em> and <em>ex-situ</em> conservation and management plan to improve the rescue and rehabilitation strategies.</p></div>","PeriodicalId":93435,"journal":{"name":"Forensic science international. Animals and environments","volume":"1 ","pages":"Article 100035"},"PeriodicalIF":0.0,"publicationDate":"2021-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666937421000342/pdfft?md5=21a76fd88a62a05b0e24dc585fc0b66a&pid=1-s2.0-S2666937421000342-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49525793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Dog owners’ supervising duty and criminal negligence – A critical review of two judgments by Swedish courts of appeal","authors":"Helena Striwing, Sirkku Sarenbo","doi":"10.1016/j.fsiae.2021.100031","DOIUrl":"10.1016/j.fsiae.2021.100031","url":null,"abstract":"<div><p>The aim of this article is to show difficulties arising when courts judge the negligence of dog owners in absence of precise legislation and typical precedents. We analyze the elements of a crime in two cases where children were objects of canine aggression, and show how the courts constructed the issue of dog owners’ negligence. In Case 1 the Court of Appeal found that a foster parent was not negligent when leaving a boy with specific needs alone with a heavily built dog that then repeatedly bit the boy to death despite the owners attempt to pull away the dog. The court also ignored the signs of substandard animal husbandry including previous severe incidents where the dog owner also was injured. In Case 2, the Court of Appeal instead found that the breed of the dog also matter, and that not even adult presence is enough to protect a child from dog attack. Because both the child and the dog could behave unpredictably, continuous observation and supervision was required. In both cases, measures to prevent the incident were available and would have been easy to carry out.</p></div>","PeriodicalId":93435,"journal":{"name":"Forensic science international. Animals and environments","volume":"1 ","pages":"Article 100031"},"PeriodicalIF":0.0,"publicationDate":"2021-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666937421000305/pdfft?md5=a421d3c1c98fc5cd84dbdb81306a7b82&pid=1-s2.0-S2666937421000305-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46169001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"E-waste forensics: An overview","authors":"Neeti Kapoor , Pradnya Sulke , Ashish Badiye","doi":"10.1016/j.fsiae.2021.100034","DOIUrl":"10.1016/j.fsiae.2021.100034","url":null,"abstract":"<div><p>E-waste or electronic waste is simply the discarded or not in use electronic or electrical gadgets or their parts. Increased use of electronics in the modern era has led to a huge increase in e-waste produced by each passing hour of the day. The growing e-waste is not out of the list in providing opportunities for criminals to carry out their illegal activities that has surged worldwide. Crimes related to e-waste includes a range of illegal trades in e-waste, environmental crimes and cyber-crimes. Even after the “deletion” or “formatting” of the storage media, the data is still present and can be retrieved and used for ulterior motives by cybercriminals. This article outlines the forensic aspects of e-waste termed as e-waste forensics, including the significant impacts of e-waste, e-waste and crimes and some preventive measures.</p></div>","PeriodicalId":93435,"journal":{"name":"Forensic science international. Animals and environments","volume":"1 ","pages":"Article 100034"},"PeriodicalIF":0.0,"publicationDate":"2021-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666937421000330/pdfft?md5=75929305c2d2915bc4566c52fe2f03da&pid=1-s2.0-S2666937421000330-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41365381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Crimes of animal cruelty in Poland: Case studies","authors":"Denis Solodov","doi":"10.1016/j.fsiae.2021.100010","DOIUrl":"10.1016/j.fsiae.2021.100010","url":null,"abstract":"<div><p>There has been a trend in recent years towards expanding welfare measures for animal protection. In Poland, criminal law punishes a wide range of behaviour, from torturing animals to not providing proper living conditions or necessary veterinary care. Animal welfare and anti-cruelty rules are enforced by special monitoring and sanction mechanisms at both national and regional levels. This is reflected in the growing number of recorded cases of animal cruelty. Nonetheless, the offenders are rarely prosecuted, and even when they are, they seldom face severe penalties. Animal cruelty prosecutions are hindered by a delayed response from law enforcement agencies, as well as investigative failures and errors in collecting evidence.</p></div>","PeriodicalId":93435,"journal":{"name":"Forensic science international. Animals and environments","volume":"1 ","pages":"Article 100010"},"PeriodicalIF":0.0,"publicationDate":"2021-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.fsiae.2021.100010","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"101706075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Edgard O. Espinoza , M. Katherine Moore , Brian C. Hamlin , Barry W. Baker , Aiden J. Espinoza
{"title":"Forensic characterization of sea turtle oil by ambient ionization mass spectrometry: Caretta caretta, Chelonia mydas, Dermochelys coriacea, Eretmochelys imbricata, Lepidochelys kempii, and Lepidochelys olivacea","authors":"Edgard O. Espinoza , M. Katherine Moore , Brian C. Hamlin , Barry W. Baker , Aiden J. Espinoza","doi":"10.1016/j.fsiae.2021.100008","DOIUrl":"10.1016/j.fsiae.2021.100008","url":null,"abstract":"<div><p>Historically the oils from sea turtles have had a high commercial demand, and this trade has contributed to an alarming decrease in sea turtle populations worldwide. Determining the species source of seized animal oils is challenging and this has hindered enforcement of the ban on trade in sea turtle oil. In this work we investigate if the chemotypes obtained from ambient ionization mass spectrometry analysis can assist in making species classifications of oil. Oil was either obtained or yielded from tissue from Green sea turtle (<em>Chelonia mydas</em>), Loggerhead sea turtle (<em>Caretta caretta</em>), Kemp's Ridley sea turtle (<em>Lepidochelys kempii</em>), Olive Ridley sea turtle (<em>Lepidochelys olivacea</em>), Hawksbill sea turtle (<em>Eretmochelys imbricata</em>), and Leatherback sea turtle (<em>Dermochelys coriacea</em>). Additionally, common oils from vegetable and animal sources were investigated to determine if any other oil sources could be confused with sea turtle oils. The oil samples were ionized both in the positive and negative mode by Direct Analysis in Real Time (DART) and the masses were measured in a time of flight mass spectrometer (AccuTOF by JEOL). Positive and negative mode mass spectra produced species-specific chemotypes and no other oils analyzed in this study produced chemotypes resembling those of sea turtle oils. We conclude that this approach is accurate and very useful for rapidly characterizing neat sea turtle oils without the need for sample derivatization.</p></div>","PeriodicalId":93435,"journal":{"name":"Forensic science international. Animals and environments","volume":"1 ","pages":"Article 100008"},"PeriodicalIF":0.0,"publicationDate":"2021-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.fsiae.2021.100008","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"107088722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Differentiation of Galliformes feather samples for species identification using ATR-FTIR spectroscopy and chemometric techniques: Application in wildlife forensics","authors":"Preeti Guleria, M. Thakar, S. Goyal","doi":"10.1016/j.fsiae.2023.100066","DOIUrl":"https://doi.org/10.1016/j.fsiae.2023.100066","url":null,"abstract":"","PeriodicalId":93435,"journal":{"name":"Forensic science international. Animals and environments","volume":"32 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"54773020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}