Forensic science international. Animals and environments最新文献_第4页

Species discrimination from blood traces using ATR FT-IR spectroscopy and chemometrics: Application in wildlife forensics 使用ATR FT-IR光谱和化学计术从血液痕迹中识别物种：在野生动物取证中的应用

Forensic science international. Animals and environments Pub Date : 2022-12-07 DOI: 10.1016/j.fsiae.2022.100060

Chandra Prakash Sharma , Sweety Sharma , Rajinder Singh

{"title":"Species discrimination from blood traces using ATR FT-IR spectroscopy and chemometrics: Application in wildlife forensics","authors":"Chandra Prakash Sharma , Sweety Sharma , Rajinder Singh","doi":"10.1016/j.fsiae.2022.100060","DOIUrl":"https://doi.org/10.1016/j.fsiae.2022.100060","url":null,"abstract":"<div><p>Efficient tools for the identification and discrimination of species are imperative in wildlife conservation since they can endow with information of species exploitation and also abet in solving problems related to forensic science. Herein, a non-destructive and rapid analytical method (ATR-FTIR Spectroscopy) coupled with PCA and PLS-DA was employed to analyze the dry blood samples for the species discrimination. Asian Elephant (<em>Elephas maximus</em>), Indian Leopard (<em>Panthera pardus fusca</em>), and Royal Bengal Tiger (<em>Panthera tigris tigris</em>) species were used to construct the chemometric models. Additionally, Domestic Pig (<em>Sus scrofa domesticus</em>) and Human (<em>Homo sapiens</em>) blood were taken for the external validation study. The evaluation results illustrate that the ATR FT-IR Spectroscopy in combination with PLS-DA model showed statistically confident discrimination among selected species from dry blood traces. ATR-FTIR spectroscopy supported with predictive models has been a robust, ideal, and suitable tool for species discrimination from dry blood traces recovered in wildlife investigations.</p></div>","PeriodicalId":93435,"journal":{"name":"Forensic science international. Animals and environments","volume":"3 ","pages":"Article 100060"},"PeriodicalIF":0.0,"publicationDate":"2022-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49840935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

DNA barcode of tilapia fish fillet from the Brazilian market and a standardized COI haplotyping for molecular identification of Oreochromis spp. (Actinopterygii, Cichlidae) 巴西市场罗非鱼鱼片的DNA条形码和用于Oreochromis spp.分子鉴定的标准化COI单倍型

Forensic science international. Animals and environments Pub Date : 2022-12-07 DOI: 10.1016/j.fsiae.2022.100059

Beatriz Miguez Nascimento , Thiago Silva de Paula , Paulo Marques Machado Brito

{"title":"DNA barcode of tilapia fish fillet from the Brazilian market and a standardized COI haplotyping for molecular identification of Oreochromis spp. (Actinopterygii, Cichlidae)","authors":"Beatriz Miguez Nascimento , Thiago Silva de Paula , Paulo Marques Machado Brito","doi":"10.1016/j.fsiae.2022.100059","DOIUrl":"10.1016/j.fsiae.2022.100059","url":null,"abstract":"<div><p>The globalized production chains of fish products have been posing increasing difficulties in carrying out proper food control on any level. In case of species identification, morphological examinations that are usually efficient to identify entire fishes, fresh or frozen, fail when applied to fish derivative products, such as fillets or canned meat. In this study we evaluated the global distribution of tilapia COI haplotypes and haplogroups, contrasting diversity in native range and areas where <em>Oreochromis</em> spp. are regarded as introduced. Introduced areas showed a lower diversity of haplotypes comparatively to native areas, evidencing that cultures in different parts of the world originated from a few strains in Africa. Afterwards, evaluating COI diversity from tilapia fillets of the Brazilian market, we concluded that diversity in Brazilian cultures is similar to other countries where they were introduced, but it was highly heterogeneous among brands. Since part of the global movement on tilapia culture relies on crossbreeding of tilapia lineages, the difficulty in defining specific strains of tilapia through DNA barcodes is thought to increase. Our haplotyping system presents a practical form of molecular identification for tilapia products that may be useful in future taxonomic reviews. However, the estimated genetic diversity of <em>Oreochromis</em> through the COI marker does not necessarily reflect the diversity of species in the group, but rather the evolutionary history of this marker. Nevertheless, given the costs and feasibility of analyzing multiple markers, such as microsatellites, the current haplotyping standard is an improvement over the previous system.</p></div>","PeriodicalId":93435,"journal":{"name":"Forensic science international. Animals and environments","volume":"3 ","pages":"Article 100059"},"PeriodicalIF":0.0,"publicationDate":"2022-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44768885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

DNA analysis and validation for species identification of seized helmeted hornbill (Rhinoplax vigil) casques 被查获的带盔犀鸟（Rhinoplax night）的DNA分析和物种鉴定

Forensic science international. Animals and environments Pub Date : 2022-12-05 DOI: 10.1016/j.fsiae.2022.100058

Chloe E.R. Hatten , Yuli S. Fitriana , Tracey-Leigh Prigge , Mohammad Irham , Hari Sutrisno , Abinawanto , Caroline Dingle

{"title":"DNA analysis and validation for species identification of seized helmeted hornbill (Rhinoplax vigil) casques","authors":"Chloe E.R. Hatten , Yuli S. Fitriana , Tracey-Leigh Prigge , Mohammad Irham , Hari Sutrisno , Abinawanto , Caroline Dingle","doi":"10.1016/j.fsiae.2022.100058","DOIUrl":"https://doi.org/10.1016/j.fsiae.2022.100058","url":null,"abstract":"<div><p>Helmeted hornbills (<em>Rhinoplax vigil</em>, J.R. Forster, 1781) are ‘Critically Endangered’ due to illegal hunting for their casques which are carved and traded for ornamental purposes. DNA species identification techniques can aid enforcement efforts, and validated wildlife forensic techniques for the species identification of <em>R. vigil</em> are needed. Here we tested multiple methods for sampling and extracting DNA from <em>R. vigil</em> casques and a validated a previously published assay using cytochrome B (cytB) primers to identity species and origin of traded casques. Phenol-chloroform: isoamyl alcohol extractions resulted in samples with higher quantity and quality of DNA than those extracted using the commercial Qiagen DNeasy Blood and Tissue kit. Samples collected from the caudal side of the casque yielded higher DNA quantity and quality than rostral and lateral sides, regardless of sampling method. We then assessed the repeatability, reproducibility, robustness, sensitivity, specificity, and phylogenetic resolution of a previously published species identification assay. We confirm the ability of this method to phylogenetically distinguish between <em>R. vigil</em> and closely related hornbills with high bootstrap support (99%). We also report the first genetic evidence of illegally traded <em>R. vigil</em> in Hong Kong using confiscated casques and provide more reference samples of <em>R. vigil</em> for future work. Overall, we provide multiple protocols for sampling and extracting DNA, and a validated species identification assay for amplifying DNA from <em>R. vigil</em> casques with potential to aid law enforcement in illegal wildlife crimes.</p></div>","PeriodicalId":93435,"journal":{"name":"Forensic science international. Animals and environments","volume":"3 ","pages":"Article 100058"},"PeriodicalIF":0.0,"publicationDate":"2022-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49840936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

GPS tracking data relates vulture mortality due to acute intoxication at a considerable distance from the site of poisoned bait consumption GPS跟踪数据涉及秃鹫因急性中毒而死亡，在距离中毒诱饵食用地点相当远的地方

Forensic science international. Animals and environments Pub Date : 2022-12-01 DOI: 10.1016/j.fsiae.2022.100052

Hristo Peshev , Emanuil Mitrevichin , Georgi Stoyanov , Atanas Grozdanov , Emilian Stoynov

引用次数: 4

Comparison of Babesia gibsoni infection in pit bull-type dogs with and without a known history of involvement in organized dogfighting 有和无有组织斗狗史的斗牛犬感染巴贝斯虫的比较

Forensic science international. Animals and environments Pub Date : 2022-12-01 DOI: 10.1016/j.fsiae.2022.100044

Laura Niestat , Maya Gupta , Rachel Touroo , Elizabeth Brandler

引用次数: 1

Forensic science and environmental offences: Litter, DNA analysis and surveillance 法医学和环境犯罪:垃圾、DNA分析和监测

Forensic science international. Animals and environments Pub Date : 2022-12-01 DOI: 10.1016/j.fsiae.2022.100042

Tersia Oosthuizen, Loene M. Howes, Rob White

{"title":"Forensic science and environmental offences: Litter, DNA analysis and surveillance","authors":"Tersia Oosthuizen, Loene M. Howes, Rob White","doi":"10.1016/j.fsiae.2022.100042","DOIUrl":"10.1016/j.fsiae.2022.100042","url":null,"abstract":"<div><p>Items of litter such as chewing gum, cigarette butts, and dog droppings are each small but collectively have considerable negative environmental impacts. Accordingly, governments at all levels have used media campaigns to raise awareness of the environmental issues associated with litter in efforts to prevent its proliferation. In a similar vein, artists have developed thought-provoking works about waste and litter, including some about the potential to identify litter culprits through DNA analysis. In a case of life imitating art, recent advances in DNA analysis techniques make possible a range of ways in which DNA taken from litter could be used, including for purposes of social regulation. This article discusses examples of the use of litter as a source of DNA for analysis and the resulting genetic surveillance. In doing so, it raises questions about proportionality and justifications for such uses of DNA analysis techniques in regard to regulation and enforcement objectives, with particular concerns about permissions, privacy and the public interest.</p></div>","PeriodicalId":93435,"journal":{"name":"Forensic science international. Animals and environments","volume":"2 ","pages":"Article 100042"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666937422000026/pdfft?md5=5222bf669088548a56c32e9af03f47e1&pid=1-s2.0-S2666937422000026-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"54772246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bone marrow fat, visceral fat, and body conditions as parameters of possible neglect in dogs with leishmaniasis 骨髓脂肪、内脏脂肪和身体状况作为利什曼病狗可能被忽视的参数

Forensic science international. Animals and environments Pub Date : 2022-12-01 DOI: 10.1016/j.fsiae.2022.100049

Hugo Ribeiro Morais , Karen Santos Março , Lívia Castanhas Bregano , Thiago Luís Magnani Grassi , Tulio Faria Seraguci , Giulia Jussiani , Guilherme Dias de Melo , Rafael Cipriano , Elisa Helena Giglio Ponsano , Gisele Fabrino Machado

{"title":"Bone marrow fat, visceral fat, and body conditions as parameters of possible neglect in dogs with leishmaniasis","authors":"Hugo Ribeiro Morais , Karen Santos Março , Lívia Castanhas Bregano , Thiago Luís Magnani Grassi , Tulio Faria Seraguci , Giulia Jussiani , Guilherme Dias de Melo , Rafael Cipriano , Elisa Helena Giglio Ponsano , Gisele Fabrino Machado","doi":"10.1016/j.fsiae.2022.100049","DOIUrl":"10.1016/j.fsiae.2022.100049","url":null,"abstract":"<div><p>This study aimed to verify the body condition parameters that can be used to characterise possible cases of neglect in dogs with visceral leishmaniasis. Fifty dogs were used in the study. The control group contained 11 dogs. Of the 39 infected dogs, 25 and 14 were included in the multisymptomatic and oligo/asymptomatic groups, respectively. The parameters evaluated included body score, body mass index, bone marrow fat percentage (Soxhlet method), bone marrow supernatant fat content (mm in a 15-ml tube), and visceral fat content. We observed that most euthanised dogs with canine leishmaniasis were multisymptomatic, implying that they were unwell and had a low body condition score. This condition is associated with low bone marrow fat content and maintenance of visceral fat reserves. Therefore, the assessment of body score and bone marrow fat content associated with visceral fat content can be used as evidence of neglect in dogs with visceral leishmaniasis.</p></div>","PeriodicalId":93435,"journal":{"name":"Forensic science international. Animals and environments","volume":"2 ","pages":"Article 100049"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666937422000099/pdfft?md5=2f9f919086cf603ae3ffd43a4a5e0201&pid=1-s2.0-S2666937422000099-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"54772291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Animal abuse in Spanish-breed fighting cocks (Gallus gallus domesticus) 西班牙斗鸡(Gallus Gallus domesticus)的动物虐待

Forensic science international. Animals and environments Pub Date : 2022-12-01 DOI: 10.1016/j.fsiae.2022.100050

Agustín Rebollada-Merino , Carmen Bárcena , Lucía Barreno , Manuel Pizarro-Díaz , Lucas Domínguez , Antonio Rodríguez-Bertos

{"title":"Animal abuse in Spanish-breed fighting cocks (Gallus gallus domesticus)","authors":"Agustín Rebollada-Merino , Carmen Bárcena , Lucía Barreno , Manuel Pizarro-Díaz , Lucas Domínguez , Antonio Rodríguez-Bertos","doi":"10.1016/j.fsiae.2022.100050","DOIUrl":"10.1016/j.fsiae.2022.100050","url":null,"abstract":"<div><p>Cockfighting is illegal both in the European Union and the United States, although fighting cock husbandry and breed preservation are allowed. Here, we describe the pathological findings observed in the carcasses of two Spanish-breed fighting cocks submitted to our laboratory after an animal abuse-suspected death. The postmortem examination revealed these cocks may have been used for cockfighting (comb, ear lobes, wattles, and chest feather removal; and trimmed spurs). The severe extensive hemorrhages observed in the cervical region of both birds, affecting C1-C4 in bird No. 1 and C8-C11 in No. 2, suggested cervical dislocation in a non-professional manner. To the best of our knowledge, this is the first report of abuse in a Spanish-breed fighting cock. The aim of this paper is to contribute to the identification of animal abuse cases in avian species which may lead to successful prosecutions in Spain and elsewhere.</p></div>","PeriodicalId":93435,"journal":{"name":"Forensic science international. Animals and environments","volume":"2 ","pages":"Article 100050"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666937422000105/pdfft?md5=0b9496a681f603a2856129a2a6406a15&pid=1-s2.0-S2666937422000105-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"54772978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Investigating the utility of a handheld X-ray fluorescence (XRF) device as a field test for suspected ivory 调查手持式x射线荧光(XRF)装置作为可疑象牙现场测试的效用

Forensic science international. Animals and environments Pub Date : 2022-12-01 DOI: 10.1016/j.fsiae.2022.100041

Mark J. McLellan , Kelly L. Carrothers , Ashley M. Spicer

{"title":"Investigating the utility of a handheld X-ray fluorescence (XRF) device as a field test for suspected ivory","authors":"Mark J. McLellan , Kelly L. Carrothers , Ashley M. Spicer","doi":"10.1016/j.fsiae.2022.100041","DOIUrl":"10.1016/j.fsiae.2022.100041","url":null,"abstract":"<div><p>Forensic or field investigators can use X-ray fluorescence (XRF) to determine the elemental profile of an item. The elemental composition of an item can subsequently be used to identify the source of that item when compared with the elemental compositions of known potential source materials. For example, illegal trafficking of animal parts, including ivory, requires investigators to identify that an item originated from an animal source. Here, we describe a quick and effective field test that enables law enforcement officers to differentiate genuine ivory, bone, and antler from fake ivory, and to seize genuine ivory items with greater certainty. We used a handheld XRF device to scan 445 items from 9 categories to represent a range of biological and non-biological materials that may be encountered in the field. We established an XRF Ivory Flowchart with ranges for ivory based on a calcium to phosphorus (Ca/P) ratio of 1.2–4.0 and a light element (LE) percentage range of 27%− 76%. Four volunteers used the XRF device to scan 21 items (9 ivory and 12 non-ivory items) in combination with the XRF Ivory Flowchart to effectively identify 97% of the ivory items and to eliminate 83% of the non-ivory items. We believe the utility of the XRF device combined with the XRF Ivory Flowchart provides law enforcement officers with a valuable tool to combat illegal ivory trafficking and can assist with efforts to eliminate the poaching of protected ivory-bearing species.</p></div>","PeriodicalId":93435,"journal":{"name":"Forensic science international. Animals and environments","volume":"2 ","pages":"Article 100041"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666937422000014/pdfft?md5=d4ef0d7826c40d7597b26239c77f6f50&pid=1-s2.0-S2666937422000014-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47394567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

COVID-19 and medicinal plants: A critical perspective COVID-19与药用植物:一个批判的视角

Forensic science international. Animals and environments Pub Date : 2022-12-01 DOI: 10.1016/j.fsiae.2022.100043

Reena V. Mathai , Manoj Kumar Jindal , Jayati Chatterjee Mitra , Santosh Kumar Sar

{"title":"COVID-19 and medicinal plants: A critical perspective","authors":"Reena V. Mathai , Manoj Kumar Jindal , Jayati Chatterjee Mitra , Santosh Kumar Sar","doi":"10.1016/j.fsiae.2022.100043","DOIUrl":"10.1016/j.fsiae.2022.100043","url":null,"abstract":"<div><p>On a global scale, the Coronavirus pandemic (COVID-19) is having a direct and indirect effect on human lives, socioeconomic conditions, and the natural environment. The measures are taken to prevent the spread of coronavirus and slowdown of economic activities could have major short and long term effects on the natural ecosystem and climate in the coming days. Based on the current scientific studies, the present perspective intends to examine the possible direct and indirect impacts of the COVID-19 pandemic on the ecosystem particularly on medicinal plants. The natural compounds obtained from medicinal plants and herbal formulations provide rich sources of novel effective measures to control viral infections. The unpredictable COVID situation has affected the environment based on several aspects which may play a key role in impact on plants. The positive perspectives of the world pandemic are a significant improvement in quality of air, reduced carbon emission, increased water purity and reduction in other types of pollution. But at the same time, the negative consequences are much more, which mainly includes increased consumption of preventive medical equipment and medical wastes due to treatment and human immortality, which is continuously endangering the medicinal plants. These wastes may affect the natural cycling process and the natural habitat of the medicinal plants which are a promising solution for the prevention of viral diseases in the years to come. Hence, this perspective will be beneficial for the possible research studies and proper implementation of the strategies that might be support the global climate sustainability.</p></div>","PeriodicalId":93435,"journal":{"name":"Forensic science international. Animals and environments","volume":"2 ","pages":"Article 100043"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8837494/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39815731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0