Species discrimination from blood traces using ATR FT-IR spectroscopy and chemometrics: Application in wildlife forensics

Forensic science international. Animals and environments Pub Date : 2022-12-07 DOI:10.1016/j.fsiae.2022.100060

Chandra Prakash Sharma , Sweety Sharma , Rajinder Singh

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Abstract

Efficient tools for the identification and discrimination of species are imperative in wildlife conservation since they can endow with information of species exploitation and also abet in solving problems related to forensic science. Herein, a non-destructive and rapid analytical method (ATR-FTIR Spectroscopy) coupled with PCA and PLS-DA was employed to analyze the dry blood samples for the species discrimination. Asian Elephant (Elephas maximus), Indian Leopard (Panthera pardus fusca), and Royal Bengal Tiger (Panthera tigris tigris) species were used to construct the chemometric models. Additionally, Domestic Pig (Sus scrofa domesticus) and Human (Homo sapiens) blood were taken for the external validation study. The evaluation results illustrate that the ATR FT-IR Spectroscopy in combination with PLS-DA model showed statistically confident discrimination among selected species from dry blood traces. ATR-FTIR spectroscopy supported with predictive models has been a robust, ideal, and suitable tool for species discrimination from dry blood traces recovered in wildlife investigations.

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使用ATR FT-IR光谱和化学计术从血液痕迹中识别物种：在野生动物取证中的应用

在野生动物保护中，识别和歧视物种的有效工具是必不可少的，因为它们可以提供物种开发的信息，也有助于解决与法医学有关的问题。本文采用非破坏性快速分析方法（ATR-FTIR光谱法），结合PCA和PLS-DA，对干血样本进行物种鉴别分析。亚洲象（Elephas maximus）、印度豹（Panthera pardus fusca）和皇家孟加拉虎（Panthera tigris tigris）物种被用于构建化学计量模型。此外，还采集了家猪（Sus scrofa domesticus）和人类（智人）的血液进行外部验证研究。评估结果表明，ATR FT-IR光谱与PLS-DA模型相结合，在从干血痕迹中选择的物种之间显示出统计上可靠的区分。预测模型支持的ATR-FTIR光谱是从野生动物调查中回收的干血痕迹中进行物种识别的一种稳健、理想和合适的工具。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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