British Journal of Ophthalmology最新文献

{"title":"Primary acquired melanosis with spill over periocular lentigo maligna: 19-year outcomes at a specialist eyelid and ocular oncology centre.","authors":"Mumta Kanda, Allan Zhifa Nghiem, Fariha Shafi, Shirin Hamed Azzam, Tarang Gupta, Claire Daniel","doi":"10.1136/bjo-2024-326691","DOIUrl":"10.1136/bjo-2024-326691","url":null,"abstract":"<p><strong>Background/aims: </strong>There is a paucity in the literature on the presentation, management and outcomes of cases where primary acquired melanosis (PAM) is associated with spill over/contiguous periocular lentigo maligna (LM). We describe experience of such cases at our eyelid and ocular oncology specialist centre.</p><p><strong>Methods: </strong>We conducted a retrospective consecutive case review of adult patients with PAM and periocular LM between 2005 and 2024 at Moorfields Eye Hospital in London. Demographic data, diagnosis, histology, imaging, treatment, surgical notes, outcomes and follow-up were collected from the electronic patient record.</p><p><strong>Results: </strong>Of 21 patients identified, 100% were Caucasian, 13 (62%) were women and the average age of diagnosis was 67 years. Grade of PAM atypia was mild in 5 (24%), moderate in 2 (9%) and severe in 14 (67%) patients. 16 (76%) patients developed melanoma (all types) and 12 (57%) patients developed conjunctival melanoma. Of those with PAM with severe atypia, 93% developed melanoma. The average time interval from diagnosis of PAM and LM to melanoma was 72 months. Melanoma recurrence occurred in 7 (44%) and metastases developed in 4 (25%) patients. Four patients died, including two from metastatic melanoma. Average follow-up length was 129 months.</p><p><strong>Conclusions: </strong>PAM with atypia, particularly severe atypia, when associated with spill over periocular LM, may have significant risk of progression to melanoma. Patients with PAM require careful eyelid examination to identify LM. Management requires multidisciplinary input, urgent biopsy and confocal microscopy if available, lower threshold for treatment and lifelong monitoring.</p>","PeriodicalId":9313,"journal":{"name":"British Journal of Ophthalmology","volume":" ","pages":"909-916"},"PeriodicalIF":3.7,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143448167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence and incidence of juvenile idiopathic arthritis-associated uveitis and medication trends in the TriNetX database.","authors":"Rayna Marshall,Jennifer Thorne,Meghan K Berkenstock","doi":"10.1136/bjo-2024-327087","DOIUrl":"https://doi.org/10.1136/bjo-2024-327087","url":null,"abstract":"AIMSTo investigate the prevalence and incidence of juvenile idiopathic arthritis (JIA)-associated uveitis (JIA-U) in the USA from 2013 to 2023 by analysing the TriNetX database. Secondary outcomes analysed included disease demographics, associated ocular complications and immunosuppressive prescription trends.METHODSPatients with JIA-U were identified in the TriNetX database, a large, multicentre research network, through International Classification of Diseases, 10th Edition codes. Data collected included patient demographics, concurrent uveitic ocular complications, incidence, prevalence and cumulative prevalence of JIA-U in the 11-year period and immunosuppressive utilisation in the JIA population.RESULTS4510 patients with JIA-U were included in this study. The cumulative prevalence and incidence of JIA-U over the 11-year period were 4.4 and 1.2 per 100 000 cases, both of which decreased over the study period. 5.5% of patients with JIA had a diagnosis of uveitis and 36.1% of patients with JIA-U had an associated uveitic ocular adverse event, including glaucoma, band keratopathy, low vision and blindness. The prevalence of immunosuppressive drug therapy in patients with JIA increased over the years 2013-2023.CONCLUSIONIn this large US database, the JIA-U prevalence and incidence rates decreased between 2013 and 2023. This may be partially attributed to the increasing prevalence of immunosuppressive drug use in patients with JIA.","PeriodicalId":9313,"journal":{"name":"British Journal of Ophthalmology","volume":"212 1","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144684089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Risk factors for bleeding and perioperative bleeding complications in oculoplastic surgery: a prospective pilot study.","authors":"Nike Annika Christensen,Nicolas Feltgen,Maren Horn,Sabine Naxer,Christian van Oterendorp,Jürgen Koscielny,Christoph Sucker,Sebastian Pfeiffer,Fabian Kück,Michael P Schittkowski","doi":"10.1136/bjo-2024-326553","DOIUrl":"https://doi.org/10.1136/bjo-2024-326553","url":null,"abstract":"BACKGROUND/AIMSClear guidelines for managing patients on antiplatelet (AP) or anticoagulant (AC) therapy undergoing oculoplastic surgery are lacking. This study investigates bleeding risk factors and evaluates the effectiveness of a standardised bleeding risk assessment in extraocular surgery.METHODSIn this prospective study, a standardised questionnaire (SQ) was employed to gather patients' bleeding histories. Associations between SQ scores and preoperative/demographic factors (AP/AC use, age, sex, comorbidities), and surgical factors (blood pressure, surgery type, primary vs revision surgery, anaesthesia type, duration, pain management) were assessed. A scoring system quantified bleeding severity. Patients were monitored postoperatively for 24 hours (eyelid procedures) to 3 or 4 days (orbital/nasolacrimal surgery).RESULTSA total of 324 patients (aged 18-92) were included (252 eyelid, 56 orbital and 16 nasolacrimal surgeries). 87 patients were using AP, AC and/or herbal supplements. Severe bleeding occurred in 35 patients, four required revision surgery, though none suffered permanent damage. Bleeding complications were significantly associated with higher SQ scores (p=0.048), acetylsalicylic acid use in eyelid surgery (p=0.038), diabetes mellitus (p=0.016), renal disease (p=0.044), general anaesthesia (p=0.001) and longer surgery duration (p=0.007). Local anaesthesia, AC use and systolic hypertension were not significantly associated with bleeding risk.CONCLUSIONThe SQ is a valuable tool for assessing bleeding risk in oculoplastic patients. Findings support future multicentre studies to develop evidence-based perioperative guidelines for AP and AC management. Limitations include cohort heterogeneity, small sample size, non-blinded design and subjective bleeding scores affecting comparability.","PeriodicalId":9313,"journal":{"name":"British Journal of Ophthalmology","volume":"45 1","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144684087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of intra-arterial chemotherapy drug regimens on globe salvage outcomes of retinoblastoma patients.","authors":"Xiaoyu He, Minglei Han, Min Zhou, Peiwei Chai, Lei Guo, Jiayan Fan, Xuyang Wen, Xianqun Fan","doi":"10.1136/bjo-2024-326452","DOIUrl":"10.1136/bjo-2024-326452","url":null,"abstract":"<p><strong>Aims: </strong>To compare the effect of three-drug regimen (melphalan, topotecan and carboplatin) with two-drug regimen (melphalan and topotecan) on the globe salvage outcomes of retinoblastoma (Rb) with intra-arterial chemotherapy (IAC).</p><p><strong>Methods: </strong>This study included 83 patients with 87 eyes received two-drug regimen IAC, and 95 patients with 97 eyes received three-drug regimen IAC. Propensity score matching was applied, and 84 matched (42:42) patients (eyes) were analysed. All Rb patients underwent an ophthalmologic examination before IAC using a wide-angle digital fundus camera, which was repeated monthly after the globe salvage management. Progression-free ocular survival (PFOS), relapse rate and local complications were analysed per affected eye. Analysis of overall survival (OS) and systemic complications was based on Rb individuals.</p><p><strong>Results: </strong>Eyes treated with three-drug regimen IAC presented with higher overall PFOS than those treated with two-drug regimen IAC (p=0.026). Stratified analysis showed PFOS rate in three-drug group was higher than two-drug group among group D eyes (88.5% vs 60.6%, p=0.009). Consistently, among group E eyes, three-drug group correlated with a better PFOS, compared with two-drug group (42.9% vs 23.5%, p=0.002). There did not present with significant differences in OS (p=0.853), relapse rate (p=0.291), incidence of moderate-severe myelosuppression (grade 3-4, p=0.564) or ophthalmic artery obstruction (p=0.998) between these two groups.</p><p><strong>Conclusions: </strong>Three-drug regimen was a superior IAC management compared with two-drug regiment, with improved progression-free ocular outcome for eyes of advanced retinoblastomas.</p>","PeriodicalId":9313,"journal":{"name":"British Journal of Ophthalmology","volume":" ","pages":"888-893"},"PeriodicalIF":3.5,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12320595/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143424554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Incidence, sociodemographic and presenting clinical features of childhood non-infectious uveitis: findings from the UK national inception cohort study.","authors":"Ameenat Lola Solebo, Salomey Kellett, Eibhlin McLoone, Harry Petrushkin, Jose Gonzalez-Martin, Jane Ashworth, Jessy Choi, Rachel F Pilling, Kate Armon, Kishore Warrier, Srilakshmi M Sharma, Jugnoo S Rahi","doi":"10.1136/bjo-2024-326674","DOIUrl":"10.1136/bjo-2024-326674","url":null,"abstract":"<p><strong>Background: </strong>We aimed to provide, through the Uveitis in Childhood National Cohort Study, population-based evidence on incidence, distribution and disease characteristics for childhood onset non-infectious uveitis.</p><p><strong>Methods: </strong>Eligible children and young people (<18 years) were those newly diagnosed with non-infectious uveitis between 1 March 2020 and 28 February 2023. Cases were identified and recruited through passive surveillance across a multicentre network. Descriptive analysis of demographic, socioeconomic and clinical characteristics at diagnosis is reported alongside incidence rates, relative rates by region and sociodemographic patterning.</p><p><strong>Results: </strong>468 cases were identified, providing a minimal national disease incidence of 1.89/100 000 (95% CI 1.72 to 2.07). Among the 255 children recruited, anterior uveitis was predominant (76.9%) and 65% of cases were bilateral. Peak incidence was at 11-15 years. Children resident in deprived areas and those from non-White ethnic backgrounds were over-represented (28% and 31% of the cohort). One in seven children (15%) had a diagnosis of juvenile idiopathic arthritis (JIA), and 5% had tubulointerstitial nephritis. Although bilaterally poor vision was uncommon (16.8%), 44.3% had lost some vision in at least one eye.</p><p><strong>Conclusions: </strong>There is a need to reconsider how best to deliver paediatric rheumatological and eye care that meets the needs of young people, as well as young children, with uveitis. The predominance of non-JIA-related uveitis calls for a shift in focus. There appears to be socioeconomic drivers of disease risk, which are worthy of future exploration and which have implications on the delivery of care for this chronic and blinding disease.</p>","PeriodicalId":9313,"journal":{"name":"British Journal of Ophthalmology","volume":" ","pages":"955-961"},"PeriodicalIF":3.5,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12320610/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143398302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterisation and prevalence of inherited retinal diseases in the Finnish population reveals enrichment of population-specific phenotypes and causative variants.","authors":"Laura Lähteenoja, Pasi Ohtonen, Aura Falck, Elisa Johanna Rahikkala","doi":"10.1136/bjo-2025-327427","DOIUrl":"10.1136/bjo-2025-327427","url":null,"abstract":"<p><strong>Aims: </strong>This study aims to assess clinical and genetic characteristics as well as the prevalence of inherited retinal dystrophies (IRD) and their subphenotypes in the Finnish founder population.</p><p><strong>Methods: </strong>A retrospective analysis of clinical and genetic data from Northern Finnish patients diagnosed with IRD between 1996 and 2023 at Oulu University Hospital, Finland, was conducted.</p><p><strong>Results: </strong>The cohort comprised 582 patients with IRD, categorised into 16 different subphenotypes. Pathogenic or likely pathogenic variants explaining IRD were identified in 36% (n=210/582) of all patients and 80% (n=210/261) of genetically tested patients with IRD. Diagnostic yields varied between different IRD subphenotypes. The genetic aetiology was most commonly confirmed in X-linked retinoschisis, severe early childhood-onset retinal dystrophy, congenital stationary night blindness and choroideremia. The lowest rates of causative variant identification were observed in cone or cone-rod dystrophy and macular dystrophy. In total, 70 pathogenic or likely pathogenic variants were identified across 39 different genes; variants in the <i>FZD4</i> and <i>RPGR</i> genes were the most prevalent. Over half of the variants were enriched in the Finnish population. The estimated total prevalence of IRDs in Northern Finland was 69.8/100 000 (1:1432). The prevalence of the most common subphenotypes was as follows: retinitis pigmentosa, 25.3/100 000; X-linked retinoschisis, 10.7/100 000; Usher syndrome, 8.9/100 000; choroideremia, 7/100 000 and cone or cone-rod dystrophy, 6/100 000.</p><p><strong>Conclusion: </strong>The Northern Finnish population exhibits an enrichment of population-specific IRD-associated variants, resulting in a high overall prevalence of IRDs and an increased prevalence of selected retinal subphenotypes, such as retinoschisis, choroideremia and Usher syndrome types 3 and 1.</p>","PeriodicalId":9313,"journal":{"name":"British Journal of Ophthalmology","volume":" ","pages":"852-857"},"PeriodicalIF":3.7,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144504886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Asymmetric thickening of the ganglion cell layer and inner plexiform layer complex correlates with metamorphopsia in epiretinal membrane patients.","authors":"Wenjia Yan,Yuntong Li,Yanqiao Huang,Qiong Wang,Shuya Ke,Miner Yuan,Xiaoyan Ding","doi":"10.1136/bjo-2025-327424","DOIUrl":"https://doi.org/10.1136/bjo-2025-327424","url":null,"abstract":"AIMSTo investigate the relationship between retinal structure symmetry and metamorphopsia in idiopathic epiretinal membrane (ERM) patients.METHODS181 consecutive ERM patients were divided into four groups based on vertical/horizontal metamorphopsia (MV/MH) presence. Clinical data, including best-corrected visual acuity (BCVA), metamorphopsia scores and optical coherence tomography (OCT)-assessed macular structure, were analysed. The Asymmetry Index (ASI), defined as the thickness difference ratio between corresponding retinal layers, was introduced. Univariate and multivariate linear regression explored relationships among BCVA, metamorphopsia and OCT patterns. Receiver operating characteristic (ROC) curves evaluated the discriminatory ability of OCT parameters for metamorphopsia.RESULTSVertical ASI (ASI-V) of the Ganglion cell layer and Inner plexiform layer complex (GCL+IPL) layer was significantly higher in MH groups (p<0.0001), while Horizontal ASI (ASI-H) was elevated in MV groups (p=0.0001). Strong correlations were found between MH and ASI-V (p<0.0001, R2=0.4536), and MV and ASI-H (p<0.0001, R2=0.4870). Multivariate analysis showed a positive association between average metamorphopsia scores and ASI-T of the GCL+IPL layer (standardised beta=0.633, p<0.001). ROC analysis demonstrated good diagnostic accuracy of ASI-V for MH (area under the curve=0.823, Youden's Index=0.669).CONCLUSIONSVertical and horizontal asymmetric thickening of the GCL+IPL layer correlates with respective metamorphopsia types. The ASI of the GCL+IPL shows diagnostic superiority for metamorphopsia and may serve as a novel biomarker. These findings suggest that asymmetric thickening of the GCL+IPL layer, rather than overall thickening per se, may serve as the pathological basis for the development of metamorphopsia in ERM patients.","PeriodicalId":9313,"journal":{"name":"British Journal of Ophthalmology","volume":"14 1","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144684284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Associations between a healthy lifestyle score and retinal neurovascular health.","authors":"Xiaomin Zeng, Ruiye Chen, Xiayin Zhang, Ting Su, Yaxin Wang, Yijun Hu, Xianwen Shang, Danli Shi, Honghua Yu","doi":"10.1136/bjo-2024-326184","DOIUrl":"10.1136/bjo-2024-326184","url":null,"abstract":"<p><strong>Aims: </strong>To investigate the associations between a healthy lifestyle score and retinal neurovascular health, and explore whether lower inflammation levels mediate these associations.</p><p><strong>Methods: </strong>This study is based on the UK Biobank. The healthy lifestyle score ranged from 0 to 6 and comprised physical activity, diet, sleep duration, smoking status, alcohol consumption and bodyweight. Outcomes included retinal diseases (age-related macular degeneration (AMD) and retinal vascular occlusion (RVO)) from hospital admission records (378 648 participants), retinal vascular metrics from retinal photography (32 226 participants) and retinal neural metrics from optical coherence tomography (42 557 participants). An INFLA-score was used to characterise inflammation levels.</p><p><strong>Results: </strong>Participants with better healthy life score (scored from 5 to 6) were associated with a 29% lower risk of AMD, 25% lower risk of RVO, 2% increase in artery-to-vein ratio (AVR), 0.22 µm increase in central retinal artery equivalent, 0.36 µm decrease in central retinal vein equivalent (CRVE), 0.004 increase in fractal dimension, 0.38 µm increase in retinal nerve fibre layer, 0.69 µm increase in ganglion cell-inner plexiform layer (GCIPL) and 0.35 µm increase in photoreceptor segment (PS) compared with those with worst lifestyle score (scored from 0 to 1) (all p_trend<0.01). In addition, INFLA-score partially mediated the associations between healthy lifestyle score and increased risk of AMD (mediated proportion (MP): 14.8%), higher AVR (MP: 12.76%), narrower CRVE (MP: 24.49%), thicker GCIPL (MP: 4.97%) and thicker PS (MP: 26.86%).</p><p><strong>Conclusion: </strong>Great adherence to a healthier lifestyle was associated with better retinal health in a dose-response manner. Lower inflammation partially mediated the association between a healthy lifestyle score and retinal health.</p>","PeriodicalId":9313,"journal":{"name":"British Journal of Ophthalmology","volume":" ","pages":"858-867"},"PeriodicalIF":3.7,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143390155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating lipid-lowering drug targets for full-course diabetic retinopathy.","authors":"Jiahui Cao, Ting Su, Shuilian Chen, Zijing Du, Chunran Lai, Kaiyi Chi, Qinyi Li, Shan Wang, Qiaowei Wu, Yunyan Hu, Ying Fang, Yijun Hu, Zhuoting Zhu, Yu Huang, Xiayin Zhang, Honghua Yu","doi":"10.1136/bjo-2024-325771","DOIUrl":"10.1136/bjo-2024-325771","url":null,"abstract":"<p><strong>Background: </strong>Implementing lipid control in patients with diabetes is regarded as a potential strategy for halting the advancement of diabetic retinopathy (DR). This study seeks to use Mendelian randomisation (MR) to assess the causal relationship between lipid traits and lipid-lowering drug targets and full-course DR (background DR, severe non-proliferative DR (NPDR) and proliferative DR (PDR)).</p><p><strong>Methods: </strong>A two-sample MR and drug target MR to decipher the causal effects of lipid traits and lipid-lowering drug targets on full-course DR, including background DR, severe NPDR and PDR, was conducted in the study. Genetic variants associated with lipid traits and genes encoding the protein targets of lipid-lowering drugs were extracted from the Global Lipids Genetics Consortium and UK Biobank. Summary-level data of full-course DR are obtained from FinnGen.</p><p><strong>Results: </strong>No significant causal relationship was found between lipid traits and full-course DR. However, in drug target MR analysis, peroxisome proliferator-activated receptor gamma (PPARG) enhancement was associated with lower risks of background DR (OR=0.12, p=0.005) and PDR (OR=0.25, p=0.006). Additionally, mediation MR analysis showed that lowering fasting insulin (p=0.015) and HbA1c (p=0.005) levels mediated most of the association between PPARG and full-course DR.</p><p><strong>Conclusions: </strong>This study reveals PPARG may be a promising drug target for full-course DR. The activation of PPARG could reduce the risk of full-course DR, especially background DR and PDR. The mechanism of the PPARG agonists' protection of full-course DR may be dependent on the glucose-lowering effect.</p>","PeriodicalId":9313,"journal":{"name":"British Journal of Ophthalmology","volume":" ","pages":"868-874"},"PeriodicalIF":3.5,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12320586/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143122203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of 24 pro-inflammatory cytokines in aqueous and serum of 94 patients without diabetes.","authors":"Stephen Jae Kim,Sapna Gangaputra,Jinsong Sheng,Shriji Patel,Archana A Nair,Sara Al Hussein Al Awamlh,John Fitzpatrick,Edward Cherney,Lindsay Veach,Crystal Nicholson","doi":"10.1136/bjo-2025-327421","DOIUrl":"https://doi.org/10.1136/bjo-2025-327421","url":null,"abstract":"BACKGROUND/AIMSTo assess 24 pro-inflammatory cytokines in the aqueous and serum of normal patients without diabetes.METHODS94 consecutive eyes of 94 patients undergoing elective vitrectomy surgery were prospectively enrolled. Blood and aqueous sampling were done in the operating room. A microparticle bead-based multiplex assay was used to measure aqueous and serum levels of: fibroblast growth factor-2, eotaxin, granulocyte colony stimulating factor (G-CSF), FMS-like tyrosine kinase 3 ligand (FLT-3L), growth-regulated alpha protein (GROa), interleukin (IL)-10, monocyte chemotactic protein (MCP)-3, macrophage-derived chemokine (MDC), soluble CD40L (sCD40L), IL-17A, interleukin-1 receptor antagonist (IL-1RA), IL-1β, IL-2, IL-4, IL-6, IL-8, interferon gamma-induced protein 10 (IP-10), MCP-1, macrophage inflammatory protein-1β, tumour necrosis factor-α, vascular endothelial growth factor (VEGF), regulated on activation normal T expressed and secreted (RANTES), platelet-derived growth factor (PDGF)-AA and PDGF-AB/BB.RESULTSA significant positive correlation between aqueous and serum levels was observed for FLT-3L (r=0.45; p<0.001) and positive trends were observed for eotaxin (r=0.27; p=0.04), MDC (r=0.34; p<0.01) and RANTES (r=0.33; p=0.007). There were no significant differences observed based on age or gender. In contrast, aqueous cytokine levels for G-CSF, GROa and MCP-1 were significantly higher in pseudophakic eyes compared with phakic eyes: G-CSF (16.52 pg/mL±22.39 vs 2.77 pg/mL±6.80, p<0.0001), GROa (12.45 pg/mL±6.54 vs 8.26 pg/mL±5.48, p=0.001) and MCP-1 (1340.51 pg/mL±565.21 vs 830.44 pg/mL±454.36, p<0.0001), respectively.CONCLUSIONSWe report the first characterisation of 24 different pro-inflammatory cytokines in 94 patients without diabetes. The absence of the crystalline lens was associated with significantly higher aqueous levels of G-CSF, GROa and MCP-1.TRIAL REGISTRATION NUMBERNCT04505566.","PeriodicalId":9313,"journal":{"name":"British Journal of Ophthalmology","volume":"18 1","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144684088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}