Bone最新文献

{"title":"Characterisation of the influence of dietary fat and sugar on bone health utilising densitometry, micro-computed tomography and histomorphometry","authors":"J. Khan ,&nbsp;H. Sadie-Van Gijsen ,&nbsp;L.M. Kotzé-Hörstmann ,&nbsp;S.H. Kotze ,&nbsp;J.I. Layman-Lemphane","doi":"10.1016/j.bone.2024.117380","DOIUrl":"10.1016/j.bone.2024.117380","url":null,"abstract":"<div><div>Obesogenic feeding can affect systemic metabolism and impact bone health and microarchitecture, but the findings of published studies often appear contradictory. This study aimed to compare the effects of a medium-fat/high-sugar (MF/HS) and a high-fat/high-fructose (HF/Fr) diet on the femora of weanling male Wistar rats, examining bone mineral content and density (BMC, BMD), cortical and cancellous bone microarchitecture and the cell populations within bone. Furthermore, we explored the correlations between circulating bone-targeting factors (in particular leptin, adiponectin and insulin) and bone parameters. Rats were assigned to one of three dietary groups (control: CON; MF/HS: OB1; HF/Fr: OB2; <em>n</em> = 12 each) for 17 weeks. Right-hand side femora were subjected to densitometry to measure BMC and BMD, and micro-computed tomography (μCT) was utilised to assess cortical and cancellous bone. Osteoblast (N.Ob), osteoclast (N.Oc), adipocyte (N.Ad) and chondrocyte numbers (N.Ch) were quantified histomorphometrically. Diet OB1 was largely beneficial to bone, while diet OB2 exerted detrimental effects on BMC, BMD, bone microarchitecture and bone cell populations. In cortical bone, N.Ob was positively correlated with BMD, cortical area and serum leptin. In cancellous bone, N.Ob was positively correlated with serum leptin and BMD, while N.Oc was negatively correlated with serum leptin. Overall, these findings support a role for endogenous circulating leptin in promoting bone formation. We conclude that the impact of different obesogenic diets may be driven by individual dietary effects on circulating factors, which may partly explain the contradictory reports in existing literature on the impact of HF and HS diets on bone.</div></div>","PeriodicalId":9301,"journal":{"name":"Bone","volume":"192 ","pages":"Article 117380"},"PeriodicalIF":3.5,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142878952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"HR-pQCT measurements of changes in periarticular bone density and microarchitecture one year after acute knee injury and after reconstructive surgery","authors":"Nathan J. Neeteson ,&nbsp;Annabel R. Bugbird ,&nbsp;Callie Stirling ,&nbsp;Nina Pavlovic ,&nbsp;Sarah L. Manske ,&nbsp;Richard E.A. Walker ,&nbsp;Steven K. Boyd","doi":"10.1016/j.bone.2024.117376","DOIUrl":"10.1016/j.bone.2024.117376","url":null,"abstract":"<div><div>ACL injuries commonly lead to post-traumatic osteoarthritis (PTOA), but the underlying mechanism is not well-understood. One theorized mechanism is pathological bone remodelling following an ACL tear, for which high-resolution peripheral quantitative computed tomography (HR-pQCT) is uniquely positioned to investigate <em>in vivo</em> in humans. In this study, we longitudinally investigate the one-year changes in periarticular bone density and microarchitecture in the human knee following an ACL tear and reconstructive surgery using data sampled from an on-going observational cohort study. We reduce the number of individual microarchitectural parameters using factor analysis and model one-year changes with mixed-effects models, adjusting for the effects of age, sex, meniscus status, and the baseline microarchitectural state. We find significant evidence of persistent bone density losses one year after both injury and surgery. We also observe significant increases in trabecular separation post-injury, indicating significant structural degradation, and significant increases in subchondral bone plate density post-surgery, a sign of early stiffening. Finally, we observe minimal significant contrasts for the effects of age, sex, and meniscus status, while we observe that the state of the microarchitecture at baseline has significant and varied effects on the subsequent changes, suggesting that the influence of PTOA risk factors on post-injury and post-surgery bone changes may be mediated through the state of the periarticular microarchitecture at injury and/or at surgery. In summary, we found that degradation of periarticular bone microarchitecture was observed post-injury, densification of the subchondral bone plate was observed post-surgery, and the state of the bone microarchitecture at baseline may mediate the influence of PTOA risk factors on post-injury microarchitectural adaptations.</div></div>","PeriodicalId":9301,"journal":{"name":"Bone","volume":"192 ","pages":"Article 117376"},"PeriodicalIF":3.5,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142878956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bone quality analysis of the mandible in alcoholic liver cirrhosis: Anatomical, microstructural, and microhardness evaluation","authors":"Teodora Rodic ,&nbsp;Eva M. Wölfel ,&nbsp;Imke A.K. Fiedler ,&nbsp;Danica Cvetkovic ,&nbsp;Katharina Jähn-Rickert ,&nbsp;Jelena Sopta ,&nbsp;Slobodan Nikolic ,&nbsp;Vladimir Zivkovic ,&nbsp;Björn Busse ,&nbsp;Marija Djuric ,&nbsp;Petar Milovanovic","doi":"10.1016/j.bone.2024.117378","DOIUrl":"10.1016/j.bone.2024.117378","url":null,"abstract":"<div><h3>Objectives</h3><div>Alcoholic bone disease has been recognized in contemporary literature as a systemic effect of chronic ethanol consumption. However, evidence about the specific influence of alcoholic liver cirrhosis (ALC) on mandible bone quality is scarce. The aim of this study was to explore microstructural, compositional, cellular, and mechanical properties of the mandible in ALC individuals compared with a healthy control group.</div></div><div><h3>Materials and methods</h3><div>Mandible bone cores of mаle individuаls with ALC (<em>n</em> = 6; age: 70.8 ± 2.5 yeаrs) and age-matched healthy controls (<em>n</em> = 11; age: 71.5 ± 3.8 yeаrs) were obtаined <em>postmortem</em> during аutopsy from the edentulous аlveolаr bone in the mandibular first molаr region аnd the mаndibulаr аngulus region of each individual. Micro-computed tomogrаphy wаs used to аssess bone microstructure. Analyses based on quаntitаtive bаckscаttered electron microscopy included the characterization of osteon morphology, osteocyte lаcunаr properties, and bone mаtrix minerаlizаtion. Composition of bone minerаl аnd collаgen phаses was assessed by Rаmаn spectroscopy. Histomorphometry wаs used to determine cellulаr аnd tissue chаrаcteristics of bone specimens. Vickers microhardness test was used to evaluate cortical bone mechanical properties.</div></div><div><h3>Results</h3><div>The ALC group showed higher closed cortical porosity (volume of pores thаt do not communicаte with the sаmple surfаce) (<em>p</em> = 0.003) and smaller lacunar area in the trabecular bone of the molar region (<em>p</em> = 0.002) compared with the Control group. The trabecular bone of the angulus region showed lower osteoclast number (<em>p</em> = 0.032) in the ALC group. There were higher carbonate content in the buccal cortex of the molar region (<em>p</em> = 0.008) and lower calcium content in the trabecular bone of the angulus region (<em>p</em> = 0.042) in the ALC group. The cortical bone showed inferior mechanical properties in the ALC cortical bony sites (<em>p</em> &lt; 0.001), except for the buccal cortex of the molar region (<em>p</em> = 0.063). There was no significant difference in cortical thickness between the groups.</div></div><div><h3>Conclusions</h3><div>Bone quality is differentially altered in ALC in two bony sites and compartments of the mandible, which leads to impaired mechanical properties.</div></div><div><h3>Clinical relevance</h3><div>Altered mandible bone tissue characteristics in patients with ALC should be considered by dental medicine professionals prior to oral interventions in these patients. Knowledge about mandible bone quality alterations in ALC is valuable for determining diagnosis, treatment plan, indications for oral rehabilitation procedures, and follow-up procedures for this group of patients.</div></div>","PeriodicalId":9301,"journal":{"name":"Bone","volume":"192 ","pages":"Article 117378"},"PeriodicalIF":3.5,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142873717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hormones synthesized by the adrenal reticulum protect bone density in premenopausal women with Cushing syndrome","authors":"Keyang Wu ,&nbsp;Siyu Yan ,&nbsp;Jieying Wan ,&nbsp;Yuanyuan Ye ,&nbsp;Yian Gu ,&nbsp;Hequn Sang ,&nbsp;Shuo Li ,&nbsp;Li Ding ,&nbsp;Hengjie Yuan ,&nbsp;Lina Chang ,&nbsp;Ming Liu ,&nbsp;Qing He","doi":"10.1016/j.bone.2024.117379","DOIUrl":"10.1016/j.bone.2024.117379","url":null,"abstract":"<div><h3>Background</h3><div>Differences in bone metabolism between patients with adrenal Cushing's syndrome (ACS) and Cushing's disease (CD) have been noted, but the impact of steroid hormones on bone metabolism remains underexplored. The purpose of this study is to explore the differences in bone metabolism between the two subtypes of Cushing's syndrome and the correlation between hormones synthesized by the adrenal reticulum and bone metabolism.</div></div><div><h3>Method</h3><div>This retrospective study included 75 premenopausal women, consisting of 33 patients with CD and 42 patients with ACS. The clinical characteristics, laboratory examination and bone metabolism differences between the two groups were analyzed. Then, 16 patients with ACS and 12 patients with CD underwent comparison of blood steroid hormone levels. Additionally, the relationship between bone mineral density (BMD) values and steroid hormone variables was analyzed.</div></div><div><h3>Results</h3><div>The serum and urinary cortisol and serum ACTH concentrations were significantly higher in patients with CD compared to those with ACS. Conversely, lumbar and femoral BMD were lower, and osteocalcin (OC) levels were elevated in the ACS group. Dehydroepiandrosterone (DHEA) and dehydroepiandrosterone-sulphate (DHEAS) had a significant positive correlation with <em>Z</em>-score in lumbar spine in both CD and ACS group. However, Z-score in femoral neck had a significant positive correlation with DHEA and DHEAS only in the ACS group.</div></div><div><h3>Conclusion</h3><div>Bone loss is more pronounced in ACS than in CD, despite higher cortisol levels in CD patients. High levels of hormones synthesized by the adrenal reticulum might be protective for bone density in premenopausal women with hypercortisolism.</div></div>","PeriodicalId":9301,"journal":{"name":"Bone","volume":"192 ","pages":"Article 117379"},"PeriodicalIF":3.5,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142873776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Macrophage migration inhibitory factor promotes heterotopic ossification by mediating ROS/HIF-1α positive feedback loop and activating Wnt/β-catenin signaling pathway” [Bone 190 (2025) 117331]","authors":"Ping Li ,&nbsp;Wensheng Zhang ,&nbsp;Jie Zhang ,&nbsp;Jie Liu ,&nbsp;Jiaming Fu ,&nbsp;Zhengnong Wei ,&nbsp;Shiyong Le ,&nbsp;Jiajia Xu ,&nbsp;Liang Wang ,&nbsp;Zhongmin Zhang","doi":"10.1016/j.bone.2024.117365","DOIUrl":"10.1016/j.bone.2024.117365","url":null,"abstract":"","PeriodicalId":9301,"journal":{"name":"Bone","volume":"192 ","pages":"Article 117365"},"PeriodicalIF":3.5,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142866597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comment on ‘Automatic AI tool for opportunistic screening of vertebral compression fractures on chest frontal radiographs: A multicenter study’","authors":"Yanxia Jia ,&nbsp;Weibo Wang ,&nbsp;Guodong Luo ,&nbsp;Chengqiang Jin","doi":"10.1016/j.bone.2024.117372","DOIUrl":"10.1016/j.bone.2024.117372","url":null,"abstract":"","PeriodicalId":9301,"journal":{"name":"Bone","volume":"195 ","pages":"Article 117372"},"PeriodicalIF":3.5,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142866594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multi-scale inferomedial femoral neck bone quality in type 2 diabetes patients with fragility fracture","authors":"Praveer Sihota ,&nbsp;Saroj Kumar ,&nbsp;Ruban Dhaliwal ,&nbsp;Piyush Uniyal ,&nbsp;Ram Naresh Yadav ,&nbsp;Vandana Dhiman ,&nbsp;Deepak Neradi ,&nbsp;Shailesh Karn ,&nbsp;Mohin Sapara ,&nbsp;Sidhartha Sharma ,&nbsp;Sameer Aggarwal ,&nbsp;Vijay G. Goni ,&nbsp;Vishwajeet Mehandia ,&nbsp;Björn Busse ,&nbsp;Deepak Vashishth ,&nbsp;Sanjay Kumar Bhadada ,&nbsp;Navin Kumar","doi":"10.1016/j.bone.2024.117375","DOIUrl":"10.1016/j.bone.2024.117375","url":null,"abstract":"<div><div>Both trabecular and cortical bone undergo changes at multiple scales. We previously demonstrated the multi-scale changes in trabecular bone quality that contribute to bone fragility in type 2 diabetes (T2D). The link between increased fragility in T2D and multi-scale changes in cortical bone and their interaction with glycation remains unclear. This study presents, first-ever, multi-scale cortical bone quality parameters in T2D patients after their first hip fracture. The study objective was to determine the association between cortical porosity (Ct.Po.), mechanical, material, and bone compositional properties in T2D. Inferomedial femoral neck (FN) bone tissue specimens were collected from patients (<em>n</em> = 10 with T2D, <em>n</em> = 25 age- and sex-matched non-diabetes controls) who underwent hip replacement surgery following the first hip fragility fracture. Bone mineral density at FN was found to be similar between groups. In T2D, Ct.Po was higher (<strong><em>p</em> = 0.038</strong>), while ultimate stress (<strong><em>p</em> = 0.021</strong>), ultimate strain (<strong><em>p</em> = 0.040</strong>), post-yield strain (<strong><em>p</em> = 0.011</strong>), toughness (<strong><em>p</em> = 0.005</strong>), yield energy (<strong><em>p</em> = 0.003</strong>), and post-yield energy (<strong><em>p</em> = 0.004</strong>) were notably lower. Tissue compositional differences included lower gravimetric mineral/matrix (<strong><em>p</em> = 0.017</strong>), higher non-enzymatic collagen cross-link ratio (NE-xLR) (<strong><em>p</em> = 0.049</strong>) and higher sugar/matrix ratio (<strong><em>p</em> = 0.042</strong>) in T2D. Fluorescent advanced glycation end-products (fAGEs) content was higher in T2D bone (<strong><em>p</em> = 0.043</strong>). At the mesoscale, the fAGEs in the bone matrix are inversely related to the yield- and ultimate strain of T2D bone, and NE-xLR is negatively correlated with yield- and ultimate- stress in the T2D group. In conclusion, study findings demonstrate that elevated glycation weakens the mechanical integrity of cortical bone by reducing its ability to absorb energy and resist deformation, thereby contributing to bone fragility in T2D. The strong association of fAGEs with lower yield strain, along with the association of NE-xLR with lower yield- and ultimate stress, establishes a causal link between AGEs and the deterioration of cortical bone mechanical properties. These findings underscore the need for strategies targeting glycation and collagen quality to mitigate fracture risk in T2D patients.</div></div>","PeriodicalId":9301,"journal":{"name":"Bone","volume":"192 ","pages":"Article 117375"},"PeriodicalIF":3.5,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142857274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Craniofacial fibrous dysplasia: A review of current literature","authors":"Sara Tuompo ,&nbsp;Riikka E. Mäkitie ,&nbsp;Mikko T. Nieminen","doi":"10.1016/j.bone.2024.117377","DOIUrl":"10.1016/j.bone.2024.117377","url":null,"abstract":"<div><h3>Introduction</h3><div>Fibrous dysplasia (FD) is a rare genetic disease, in which normal bone is replaced by fibro-osseus tissue. Disease severity varies greatly from single monostotic lesions to widespread polyostotic disease. Craniofacial lesions are common and, due to the complex anatomy, can cause several disabling symptoms and local extra-skeletal complications. Since relatively rare, reported cases of craniofacial FD (CFD), specifically, are few and the appropriate management remains incompletely defined.</div></div><div><h3>Methodology</h3><div>We performed a systematic literature review following the PRISMA guidelines for articles considering CFD in the years 2010–2023. The search was conducted in the PubMed and OVID Medline libraries. Altogether 193 articles were retrieved and reviewed.</div></div><div><h3>Results</h3><div>Overall, the clinical presentation of CFD is highly variable depending on site and extent of lesion, with pain, deformity or impairment of function being most common. Diagnoses are often incidental and primarily based on CT imaging, while more extensive imaging, genetic studies and lesion biopsies are reserved for unclear cases. Asymptomatic patients are suitable for observation, while symptomatic or widespread disease may require more active approach with medical or surgical treatment. Follow-up is encouraged in all patients to observe possible lesion reactivation, late-stage complications and, though rarely, malignant transformation. Management should be individually tailored with a multidisciplinary team and wholesome consideration of individual needs.</div></div><div><h3>Conclusions</h3><div>This review provides an updated discussion on craniofacial FD with focus on improved understanding of disease pathophysiology, appropriate line of surgical management, and new potential means of medical treatment.</div></div>","PeriodicalId":9301,"journal":{"name":"Bone","volume":"192 ","pages":"Article 117377"},"PeriodicalIF":3.5,"publicationDate":"2024-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142840608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combining systemic and local osteoporosis treatments: A longitudinal in vivo microCT study in ovariectomized rats","authors":"Vincent A. Stadelmann ,&nbsp;Estelle Gerossier ,&nbsp;Ulrike Kettenberger ,&nbsp;Dominique P. Pioletti","doi":"10.1016/j.bone.2024.117373","DOIUrl":"10.1016/j.bone.2024.117373","url":null,"abstract":"<div><h3>Introduction</h3><div>Managing osteoporotic patients at immediate fracture risk is challenging, in part due to the slow and localized effects of anti-osteoporotic drugs. Combining systemic anti-osteoporotic therapies with local bone augmentation techniques offers a promising strategy, but little is known about potential interactions. We hypothesized that integrating systemic treatments with local bone-strengthening biomaterials would have an additive effect on bone density and structure. This study investigated interactions and synergies between systemic therapies and injectable biomaterials, HA2 and HA2-ZOL, designed for local bone strengthening. HA2-ZOL incorporates Zoledronate, a bisphosphonate, to enhance anti-resorptive effects. These materials were tested in an in vivo rat model of osteoporosis using microCT and histology.</div></div><div><h3>Methods</h3><div>Thirty-six ovariectomized Wistar rats were treated systemically with vehicle (VEH), alendronate (ALN), or parathyroid hormone (PTH). One week later, their tibiae were randomly assigned to local treatment groups: HA2, HA2-ZOL, or NaCl control. Bilateral injections targeted metaphyseal trabecular bone, with microCT scans tracking changes over 8 weeks. Regions of interest (ROIs) were identified and analyzed for bone volume fraction (BV/TV), tissue mineral density (TMD), and trabecular morphology. Histological analyses were performed at week 8 to assess bone structure and mineral inclusions.</div></div><div><h3>Results</h3><div>VEH animals with NaCl injections experienced marked bone loss, partially mitigated by ALN and PTH. HA2 injections increased BV/TV by factors of 2.5 to 3.4 across treatments compared to baseline, with effects confined to the injected material. HA2-ZOL amplified this response, with BV/TV increases up to 4.8-fold, particularly in VEH and PTH animals. The effects peaked at 2–4 weeks post-injection, followed by remodeling and restoration. Both local treatments increased trabecular thickness, with HA2-ZOL showing slower post-peak resorption.</div></div><div><h3>Discussion</h3><div>HA2 injections significantly densified bone, independent of systemic therapy. Zoledronate in HA2-ZOL enhanced bone formation and delayed resorption in control and PTH animals, but offered no additional benefit when combined with systemic bisphosphonate. These findings support the hypothesis of an additive effect, suggesting that injectable hydrogels with localized drug delivery can complement systemic therapies by rapidly increasing local bone density, thereby potentially preventing fractures in high-risk osteoporotic patients.</div></div>","PeriodicalId":9301,"journal":{"name":"Bone","volume":"192 ","pages":"Article 117373"},"PeriodicalIF":3.5,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142831256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Male Down syndrome Ts65Dn mice have impaired bone regeneration","authors":"Kirby M. Sherman ,&nbsp;Catrina J. Silveira ,&nbsp;Mingquan Yan ,&nbsp;Ling Yu ,&nbsp;Abigail Leon ,&nbsp;Kasey Klages ,&nbsp;Lauren G. White ,&nbsp;Hannah M. Smith ,&nbsp;Sarah M. Wolff ,&nbsp;Alyssa Falck ,&nbsp;Ken Muneoka ,&nbsp;Regina Brunauer ,&nbsp;Dana Gaddy ,&nbsp;Larry J. Suva ,&nbsp;Lindsay A. Dawson","doi":"10.1016/j.bone.2024.117374","DOIUrl":"10.1016/j.bone.2024.117374","url":null,"abstract":"<div><div>Trisomy of human chromosome 21 (Ts21) individuals present with a spectrum of low bone mineral density (BMD) that predisposes this vulnerable group to skeletal injuries. To determine the bone regenerative capacity of Down syndrome (DS) mice, male and female Dp16 and Ts65Dn DS mice underwent amputation of the digit tip (the terminal phalanx (P3)). This is a well-established mammalian model of bone regeneration that restores the amputated skeletal segment and all associated soft tissues. P3 amputation was performed in 8-week-old male and female DS mice and WT controls and followed by <em>in vivo</em> μCT, histology and immunofluorescence. Following P3 amputation, the bone degradation phase was attenuated in both Dp16 and Ts65Dn males. In Dp16 males, P3 regeneration was delayed but complete by 63 days post amputation (DPA); however, male Ts65Dn exhibited attenuated regeneration by 63 DPA. In both Dp16 and Ts65Dn female DS mice, P3 regenerates were indistinguishable from WT by 42 DPA. In Ts65Dn males, osteoclasts and eroded bone surface were significantly reduced, and osteoblast number significantly decreased in the regenerating digit. In Ts65Dn females, no significant differences were observed in any osteoclast or osteoblast parameter. Like Ts21 individuals and DS mice with sex differences in bone mass, these data expand the characteristic sexually dimorphism to include bone resorption and regeneration in response to skeletal injury in Ts65Dn mice. These observations suggest that sex differences contribute to the poor bone healing of DS and compound the increased risk of bone injury in the Ts21 population.</div></div>","PeriodicalId":9301,"journal":{"name":"Bone","volume":"192 ","pages":"Article 117374"},"PeriodicalIF":3.5,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142830205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}