British microbiology research journal最新文献_第5页

Molecular Identification, Bioactivity Screening and Metabolic Fingerprinting of the Actinomycetes of Chenab River Sediments 奇纳布河沉积物中放线菌的分子鉴定、生物活性筛选及代谢指纹图谱研究

British microbiology research journal Pub Date : 2016-01-10 DOI: 10.9734/bmrj/2016/28805

M. Cheema, A. Fatima, I. Sajid

引用次数: 1

Bacteria Associated with Selected Rivers in Akure, Nigeria and their Alkysulphatase Activity/Production 与尼日利亚阿库雷选定河流有关的细菌及其烷基硫酸酶活性/生产

British microbiology research journal Pub Date : 2016-01-10 DOI: 10.9734/bmrj/2016/23198

D. Arotupin, A. Yusuf

引用次数: 3

Lactobacilli Cultures against Ochratoxin A Producing Moulds Isolated from Cocoa in the South West Region of Cameroon 喀麦隆西南地区可可中抗赭曲霉毒素A产霉菌的乳酸菌培养

British microbiology research journal Pub Date : 2016-01-10 DOI: 10.9734/BMRJ/2016/25072

Bertrand Tatsinkou Fossi, B. Ngah, G. T. Nchanji, S. Wanji, R. Ndjouenkeu

{"title":"Lactobacilli Cultures against Ochratoxin A Producing Moulds Isolated from Cocoa in the South West Region of Cameroon","authors":"Bertrand Tatsinkou Fossi, B. Ngah, G. T. Nchanji, S. Wanji, R. Ndjouenkeu","doi":"10.9734/BMRJ/2016/25072","DOIUrl":"https://doi.org/10.9734/BMRJ/2016/25072","url":null,"abstract":"The presence of ochratoxin A (OTA) in cocoa beans is a major health concern, due to its deleterious effects on humans and animals. During the traditional processing and storage of cocoa, fungi contamination occurs. Many of these fungi, produce mycotoxins that can cause acute or chronic intoxication and damage to human and animals after ingestion of the contaminated food and feed. The bio-control of ochratoxigenic moulds by lactic acid bacteria (LAB) could provide a safer alternative compared to the use of fungicides. The present study aims at investigating the potential of selected lactobacilli strains to inhibit the growth of OTA producing moulds. Cocoa samples were collected in the South West region of Cameroon. The mycoflora of cocoa beans were isolated and identified using phenotypic characteristics on Potato Dextrose Agar (PDA) and microscopy after application of lactophenol cotton blue stain. LAB used were isolated from fermented palm and raffia wines and fermented pineapple juice by pour plate method on De Man Rogosa and Sharpe Agar. Phenotypic identification of LAB were identified using API 50 CHL kit. Colony PCR was used to confirm whether the isolates belong to LAB group. The inhibitory activity of Lactobacillus sp against ochratoxigenic moulds was carried out on agar using the overlay method and in broth by turbidimetric analysis. Aspergillus sp were the most prevalent fungi in cocoa samples. Aspergillus flavus , Aspergillus ochraceus, Aspergillus niger were isolated in all samples. Lactobacillus. plantarum exhibited the highest inhibitory activity against OTA producing moulds. The percentage of inhibition was ranged from 10 to 46.5%. A. ochraceus was the most susceptible mould with the various LAB isolates. The quantification of OTA using ELISA kit showed significant reduction in OTA production (p<0.05) during mixed culture of moulds with lactobacilli. Lactobacilli from fermented drinks could be used for biological control of OTA producing fungi.","PeriodicalId":9269,"journal":{"name":"British microbiology research journal","volume":"4 1","pages":"1-16"},"PeriodicalIF":0.0,"publicationDate":"2016-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84880721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Strain Development of Aspergillus brasiliensis Using Physical and Chemicals Mutagenesis for Possible Overproduction of Xylanase, Amylase, Protease and Cellulase under Submerged Fermentation (SmF) 木聚糖酶、淀粉酶、蛋白酶和纤维素酶在巴西曲霉深层发酵条件下的物化诱变选育

British microbiology research journal Pub Date : 2016-01-10 DOI: 10.9734/bmrj/2016/22953

H. Ho, Muna Abduljubar

引用次数: 2

Differential Expression Pattern of Heat Shock Protein Genes in Toxigenic and Atoxigenic Isolate of Aspergillus flavus 黄曲霉产毒分离物和产氧分离物热休克蛋白基因的差异表达模式

British microbiology research journal Pub Date : 2016-01-10 DOI: 10.9734/BMRJ/2016/25368

R. Thakur, S. Tiwari, J. Shankar

引用次数: 4

Diluent Mitigates the Inhibitory Effect of Zinc Oxide Nanoparticles on Escherichia coli and Staphylococcus aureus 稀释剂减轻氧化锌纳米颗粒对大肠杆菌和金黄色葡萄球菌的抑制作用

British microbiology research journal Pub Date : 2016-01-10 DOI: 10.9734/bmrj/2016/23252

S. Eduok, N. Asamudo

引用次数: 1

Microbiological Examination of Household Kitchen Sponges from Three Communities in Ikwuano L. G. A, Umuahia, Abia State Nigeria 尼日利亚阿比亚州乌穆阿希亚州Ikwuano L. G. A三个社区家用厨房海绵的微生物检测

British microbiology research journal Pub Date : 2016-01-10 DOI: 10.9734/bmrj/2016/19952

C. Obi, C. Ndukwu

引用次数: 2

Prevalence and Antibiotic Susceptibility of Enterobacteriaceae from Fish Samples in Allahabad, India 印度阿拉哈巴德市鱼类中肠杆菌科细菌的流行及抗生素敏感性分析

British microbiology research journal Pub Date : 2016-01-10 DOI: 10.9734/BMRJ/2016/28284

Shreya Dubey, P. Saini, Chitralekha, Priyanka Singh

{"title":"Prevalence and Antibiotic Susceptibility of Enterobacteriaceae from Fish Samples in Allahabad, India","authors":"Shreya Dubey, P. Saini, Chitralekha, Priyanka Singh","doi":"10.9734/BMRJ/2016/28284","DOIUrl":"https://doi.org/10.9734/BMRJ/2016/28284","url":null,"abstract":"Aims: The present study was conducted aiming at the isolation of the pathogenic bacteria from the family enterobacteriaceae in raw, edible and apparently healthy fishes, from different fish markets of Allahabad. Study Design: Forty fish specimens were collected from eight different locations of Allahabad region. Isolation was done using selective plating. Differentiation and characterization of different isolates was based on their growth characteristics on specific culture media, their biochemical confirmatory tests and Gram-staining reactions. Place and Duration of Study: Fish samples were collected from eight different locations of Allahabad during January to May 2016 . Methodology: Isolation of enterobacteriaceae was done according to ISO Standard; ISO 21528-1:2004 and were confirmed by different set of biochemical tests and carbohydrate fermentation tests. Total soluble proteins of the organisms were estimated by Biuret method. Antibiotic susceptibility of the isolates was tested against amphicillin, streptomycin and ciprofloxacin at different concentrations. Results: Strains of Klebsiella planticola , Citrobacter youngae , Citrobacter freundii , E. coli , Klebsiella ornithinolytica and Klebsiella pneumonia were identified out of the 56 isolates obtained. All the isolated strains organisms were susceptible to antibiotics such as amphicillin, streptomycin and ciprofloxacin at different concentrations of 10, 50, 80 and 100 m g/ml. The virulent proteins were highest in K. ornitholytica (103 mg) followed by Citrobacter sp (102.8 mg/ml) . Conclusion: Fishes sold in the fish markets of Allahabad showed presence of pathogenic bacteria especially from the family enterobacteriaceae; indicate poor hygienic conditions as well as improper storage environment. The results concluded that E. coli. (48.20%) represented the major part of bacterial flora, on the fish followed by Klebsiella spp. (30.2%).","PeriodicalId":9269,"journal":{"name":"British microbiology research journal","volume":"1 1","pages":"1-8"},"PeriodicalIF":0.0,"publicationDate":"2016-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89593865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection of Dengue Fever Virus Serotype – 4 by using One-Step Real-Time RT-PCR in Hodeidah, Yemen 用一步实时RT-PCR检测也门荷台达登革热病毒血清型- 4

British microbiology research journal Pub Date : 2016-01-10 DOI: 10.9734/BMRJ/2016/24380

Murad Alahdal, Jamal Al-Shabi, Mahmoud Ogaili, Q. Abdullah, S. Alghalibi, A. Jumaan, M. Al-Kamarany

引用次数: 8

Incidence of Antibiotic Resistant E. coli Isolated from Drinking Water Sources in Ondo, Southwestern Nigeria 尼日利亚西南部翁多饮用水水源中分离出的耐抗生素大肠杆菌的发病率

British microbiology research journal Pub Date : 2016-01-10 DOI: 10.9734/BMRJ/2016/26876

O. Aromolaran, Olubunmi Adesola-Famade, Olayinka Omoseyin

{"title":"Incidence of Antibiotic Resistant E. coli Isolated from Drinking Water Sources in Ondo, Southwestern Nigeria","authors":"O. Aromolaran, Olubunmi Adesola-Famade, Olayinka Omoseyin","doi":"10.9734/BMRJ/2016/26876","DOIUrl":"https://doi.org/10.9734/BMRJ/2016/26876","url":null,"abstract":"Aim: The aim of this study was to determine the incidence and antibiotic resistant Escherichia coli in Methodology: Fifty two (52) water samples were collected from streams and wells within and around Ondo town. Total aerobic mesophilic and coliform bacteria were determined by standard pour plate and multiple tube fermentation techniques respectively. Escherichia coli was isolated by cultivating on Eosin methylene blue (EMB) agar and tested for resistance to eight antibiotics by Kirby Bauer disc diffusion method. Results: Total mesophilic count in the well and stream water samples were between 0.01 x 10 5 -8.76 x 10 5 cfu/ml and 1.31 x 10 5 - 4.20 x 10 5 cfu/ml respectively. The MPN/100 ml of the water: well (0.40 - >160) and streams (0.70 - >160). E. coli was confirmed present in 67.74% of all the well water and 71.43% of the entire stream. 86.11% of all the isolates were resistant to beta-lactam class of antibiotics, nitrofurantoins (11.11%), aminoglycosides (2.78%) and fluoroquinolones (2.78%). 2.78% were resistant to three classes of antibiotics (nitrofurantoins, aminoglycosides and beta-lactam). 30.56% were resistant to ampicillin, while all the isolates were sensitive to ciprofloxacin. Conclusion: There is need for good hygiene practices and indiscriminate use of antibiotics should be discouraged, in order to reduce the release of antibiotic resistant E. coli to the environment.","PeriodicalId":9269,"journal":{"name":"British microbiology research journal","volume":"11 1","pages":"1-7"},"PeriodicalIF":0.0,"publicationDate":"2016-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89983595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2