木聚糖酶、淀粉酶、蛋白酶和纤维素酶在巴西曲霉深层发酵条件下的物化诱变选育

H. Ho, Muna Abduljubar
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引用次数: 2

摘要

目的:木聚糖酶通常参与由半纤维素组成的木聚糖的完全水解,两个突变体在紫外线照射25分钟和EMS处理60分钟后,淀粉酶活性也有所增强,其产量分别为0.481±0.007 U/mL和0.304±0.040 U/mL,而野生型的产量分别为0.232±0.021 U/mL。值得注意的是,与野生型相比,突变体的活性增加了107%和31%。然而,在本研究中,经过物理和化学诱变,没有检测到蛋白酶和纤维素酶可能过量产生。结论:在SmF条件下,利用农用麦麸残渣生产木聚糖酶和淀粉酶的突变体有望以更经济的方式满足工业需求。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Strain Development of Aspergillus brasiliensis Using Physical and Chemicals Mutagenesis for Possible Overproduction of Xylanase, Amylase, Protease and Cellulase under Submerged Fermentation (SmF)
Aims: Xylanase is commonly involved in the complete hydrolysis of xylan which consisted of hemicelluloses the enhancement in amylase activity was also observed in two mutants after UV exposure for 25 minutes and EMS treatment for 60 minutes with the production of 0.481±0.007 U/mL and 0.304±0.040 U/mL compared to wild type with 0.232±0.021 U/mL, respectively. Notably, there were increment of 107% and 31% in activity in mutants compared to wild type. Nevertheless, no possible overproduction of protease and cellulase was detected after the physical and chemical mutagenesis in this study. Conclusion: In a nutshell, the mutants of A. brasiliensis with enhanced production of xylanase and amylase were anticipated to fulfill the industrial demand in more economical approach using agro-residual waste of wheat bran under SmF.
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