British microbiology research journal最新文献_第6页

Hand Contamination among Food Handlers 食物处理人员的手污染

British microbiology research journal Pub Date : 2016-01-10 DOI: 10.9734/BMRJ/2016/24845

H. Allam, M. Al-Batanony, A. Seif, E. Awad

{"title":"Hand Contamination among Food Handlers","authors":"H. Allam, M. Al-Batanony, A. Seif, E. Awad","doi":"10.9734/BMRJ/2016/24845","DOIUrl":"https://doi.org/10.9734/BMRJ/2016/24845","url":null,"abstract":"Introduction: Food handlers act as a vehicle for microorganisms causing a potential risk to the public health. Hands contamination of food handlers can be used as an indicator of their behavior regarding food-related practice and personal hygiene. Aim of the Study: To assess hand contamination among food handlers working in Menoufia University and Shebin Al-Kom Educational hospital kitchens and the effect of a health education session over them. Subjects and Methods: A seventy two food handlers were the target group of this study. A prestructured questionnaire was filled including socio-demographic data beside questions regarding risk factors for contamination and personal hygiene practices during dealing with food. Hand rinse sample was taken from each participant to detect the contaminants. A brief and simple health education session was held regarding personal hygiene practices. Three months later, the same kitchen was revisited to appraise the participants’ hygiene compliance where another hand sample rinses were taken. Results: Both Staphylococcus Epidermidis and Escherichia coli (41.7%) followed by Staphylococcus aureus (29.2%) were the prevalent organisms. A significant reduction in hand","PeriodicalId":9269,"journal":{"name":"British microbiology research journal","volume":"19 1","pages":"1-8"},"PeriodicalIF":0.0,"publicationDate":"2016-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89492886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 17

Extended Spectrum β-lactamases in Clinical Isolates of Escherichia coli and Enterobacter cloacae Collected from Nablus District - Palestine 巴勒斯坦纳布卢斯地区大肠杆菌和阴沟肠杆菌临床分离株β-内酰胺酶的广谱分析

British microbiology research journal Pub Date : 2016-01-10 DOI: 10.9734/BMRJ/2016/27892

M. Al-Masri, N. Abu-Hasan, Maha Jouhari

{"title":"Extended Spectrum β-lactamases in Clinical Isolates of Escherichia coli and Enterobacter cloacae Collected from Nablus District - Palestine","authors":"M. Al-Masri, N. Abu-Hasan, Maha Jouhari","doi":"10.9734/BMRJ/2016/27892","DOIUrl":"https://doi.org/10.9734/BMRJ/2016/27892","url":null,"abstract":"Aim: To determine the prevalence of extended-spectrum β-lactamases (ESBLs) among clinical isolates of Escherichia coli (E. coli) and Enterobacter cloacae (E. cloacae) in Nablus district. Methodology: In this prospective study carried out at An-Najah National University, a total of 161 bacterial isolates were collected during a12-month period in Nablus district in Palestine. To detect ESBLs, the isolates were examined by combination disc method. PCR was used to detect blaCTXM, blaTEM and blaSHV genes in 32 representative ESBL-producer E. coli isolates. Results: Using combination disc method, ESBL enzymes were detected in 73 out 153 (47.7%) E. coli and in 1 out of 8 (12.5%) E. cloacae isolates. No significant association of ESBL-producer E. coli was observed with types of collected specimens, gender, hospital ward, outpatient, or medical source. Among 32 representative E. coli ESBL-positive, blaCTX-M, blaTEM and blaSHV genes were detected in 30 (93.8%), 2 (6.3%) and 1 (3.1%), respectively. Two new variants of ESBLs (PALTEM137b and PALSHV-2a') were identified. A unique E. cloacae isolate expressing inducible Short Research Article Al-Masri et al.; BMRJ, 16(3): 1-7, 2016; Article no.BMRJ.27892 2 class C B-lactamase was also detected. Conclusions: In Nablus region, high frequencies of ESBLs were found among E. coli bacteria isolated from outpatients and inpatients. blaCTX-M is the predominant gene among ESBL producers. New variants of ESBLs were found.","PeriodicalId":9269,"journal":{"name":"British microbiology research journal","volume":"11 1","pages":"1-7"},"PeriodicalIF":0.0,"publicationDate":"2016-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88095527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Analysis of Influenza A/Beijing/353/89 Virus Matrix Gene Sequence and Identification of Replaceable Amino Acids as Natural Mutations in M2 Protein 甲型流感/北京/353/89病毒基质基因序列分析及M2蛋白自然突变替代氨基酸鉴定

British microbiology research journal Pub Date : 2016-01-10 DOI: 10.9734/bmrj/2016/27447

Yongxin Zhang, Monica Zhang, Ying Wang, X. Gilmore, I. Mbawuike

引用次数: 0

Isolation, Characterization, and Identification of Yeasts Associated with Foods from Assiut City, Egypt 埃及Assiut市食品相关酵母的分离、特性和鉴定

British microbiology research journal Pub Date : 2016-01-10 DOI: 10.9734/bmrj/2016/24170

S. Khattab, A. Abdel-Hadi, N. Abo-Dahab, O. Atta

{"title":"Isolation, Characterization, and Identification of Yeasts Associated with Foods from Assiut City, Egypt","authors":"S. Khattab, A. Abdel-Hadi, N. Abo-Dahab, O. Atta","doi":"10.9734/bmrj/2016/24170","DOIUrl":"https://doi.org/10.9734/bmrj/2016/24170","url":null,"abstract":"The objective of this work was to isolate, characterize physiologically, and identify yeasts associated with foods from Assiut city, Egypt. Fifty-two colonies of yeasts associated with orange, mandarin, tomato, squash, sobia drink, mango juice, sugarcane juice, yogurt and buttermilk samples, collected from Assiut City, Egypt, were isolated. Out of which, Eleven isolates were selected randomly and subjected to morphological, biochemical studies and molecular identification techniques employing sequence of internal transcribed spacer (ITS) regions and partial D1/D2 large-subunit domains of the 26S ribosomal RNA. Identified yeasts were belonged to six genera and species; four species belonged to ascomycetes: Debaryomyces hansenii (five isolates), Saccharomyces cerevisiae (one isolate), Candida tropicalis (one isolate), and Pichia (one isolate). In addition, two yeasts species belonged to basidiomyces: Rhodotorula mucilaginosa (two isolates), and Trichosporon dulcitum (one isolate). In spite of low frequency of yeasts isolates on the tested food, mango juice and buttermilk showed the higher sources for incidences during this study.","PeriodicalId":9269,"journal":{"name":"British microbiology research journal","volume":"1 1","pages":"1-10"},"PeriodicalIF":0.0,"publicationDate":"2016-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86430783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Lactic Acid Bacteria Composition of Type II Sourdough Produced in Nigeria 尼日利亚产II型酵母的乳酸菌组成

British microbiology research journal Pub Date : 2016-01-10 DOI: 10.9734/bmrj/2016/16631

M. Dashen, S. Ado, J. Ameh, C. Whong

{"title":"Lactic Acid Bacteria Composition of Type II Sourdough Produced in Nigeria","authors":"M. Dashen, S. Ado, J. Ameh, C. Whong","doi":"10.9734/bmrj/2016/16631","DOIUrl":"https://doi.org/10.9734/bmrj/2016/16631","url":null,"abstract":"Aim: The aim of the study was to determine the lactic acid bacteria composition of type II sourdough produced in Nigerian from different brands of wheat flour. Study Design: Sourdoughs were produced by spontaneous fermentation at 31°C and 40°C for five days from three popular brands of Nigerian wheat flour. Lactic acid bacteria were isolated and identified from the sourdoughs using API 50 CH. Methodology: Doughs were prepared from three popular brands of wheat flour and allowed to ferment spontaneously at 31°C and 40°C for five days. Lactic acid bacteria cou nts, fungal counts and aerobic plate counts were carried out. The lactic acid bacteria were identified using the API 50 CH. Data obtained were subjected to statistical analysis using ANOVA. Significant differences among samples were evaluated by Duncan multiple – range test. Results: The results obtained showed that the mean lactic acid bacteria counts were 6.462±0.74, 6.471±0.62 and 6.826±0.68 log CFU/g after five days of fermentation at 31°C while the counts were 6.878±0.99, 6.728±0.95 and 7.051±1.04 log CFU/g after five days of fermentation at 40°C. Lactobacillus plantarum (34%), Lactobacillus brevis (29%) , Lactobacillus pentosus (18%), Pediococcus pentocaseus (9%), Lactobacillus buchneri (3%), Lactobacillus collinoides (3%), Lactobacillus fermentum (3%) and Pediococcus acidilactici (3%) were isolated and identified. Conclusion: There is no known published data on lactic acid bacteria composition of type II sourdough produced in Nigeria; findings of this work will assist to bridge this information gap. Knowledge of the lactic acid bacteria of the sourdough will help in the development of starter cultures for improvement of the nutritional and organoleptic qualities and shelflife of a wide variety of Nigerian baked products.","PeriodicalId":9269,"journal":{"name":"British microbiology research journal","volume":"62 1","pages":"1-10"},"PeriodicalIF":0.0,"publicationDate":"2016-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87397163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Microbial Assessment of Water Used by the Residents of Kabianga in Kericho, Kenya from the Different Sources 肯尼亚Kericho Kabianga居民从不同来源用水的微生物评估

British microbiology research journal Pub Date : 2016-01-10 DOI: 10.9734/BMRJ/2016/27557

K. Douglas, J. Mwangi

{"title":"Microbial Assessment of Water Used by the Residents of Kabianga in Kericho, Kenya from the Different Sources","authors":"K. Douglas, J. Mwangi","doi":"10.9734/BMRJ/2016/27557","DOIUrl":"https://doi.org/10.9734/BMRJ/2016/27557","url":null,"abstract":"Aim: The aim of the research was to assess water quality from different sources used by residents of Kabianga. Study Design: The research employed experimental design. Methodology: Techniques used in this research project included test for indicator organism preferable E. coli, total viable count, and enumeration of filamentous fungi and yeasts. For bacteriological quality of the water, indicator organisms were used to indicate the presence of pathogenic microorganisms. Results: The results showed that water obtained from springs and wells were safe for human usage and consumption as it was free from indicators of contamination. The presence of colorless colonies in well water was not considered hazardous because the colonies were well below the Original Research Article Douglas and Mwangi; BMRJ, 16(6): 1-8, 2016; Article no.BMRJ.27557 2 lethal colony number which is set to be a hundred colonies per plate. However the microbial populations identified showed that the river water was contaminated with E. coli an indicator of microbial contamination of water sources. Conclusion: From the findings it can be concluded that river water is likely to be unsafe for use, especially to people who are immunocompromised as they may suffer from diarrheal related diseases. Spontaneous outbreaks related to water-borne diseases in these area, could also be attributed to increase in the number of indicator organisms. There is need for policy makers and implementers to initiate corrective measures to reduce contamination.","PeriodicalId":9269,"journal":{"name":"British microbiology research journal","volume":"69 1","pages":"1-8"},"PeriodicalIF":0.0,"publicationDate":"2016-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77513505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Study of Bacteriological and Physicochemical Characteristics in Soils of Auto-mechanic and none Auto-mechanic Workshop Soils from Selected Areas in Calabar Metropolis 卡拉巴尔市汽车修理厂和非汽车修理厂土壤细菌学和理化特性研究

British microbiology research journal Pub Date : 2016-01-10 DOI: 10.9734/BMRJ/2016/23596

D. Tiku, B. Asikong, S. Idire

引用次数: 1

Latent Toxoplasmosis is Not a Risk Factor for Pregnancy-induced Hypertension 潜伏弓形虫病不是妊娠高血压的危险因素

British microbiology research journal Pub Date : 2016-01-10 DOI: 10.9734/bmrj/2016/23770

A. El-Henawy, H. El-Nahas, Mostafa Alkhiary

{"title":"Latent Toxoplasmosis is Not a Risk Factor for Pregnancy-induced Hypertension","authors":"A. El-Henawy, H. El-Nahas, Mostafa Alkhiary","doi":"10.9734/bmrj/2016/23770","DOIUrl":"https://doi.org/10.9734/bmrj/2016/23770","url":null,"abstract":"Aims: To compare between Toxoplasma IgG antibody seroprevalence in pregnancy-induced hypertensive females (cases) versus normotensive pregnant females (control), and to identify potential risk factors in Toxoplasma infected patients. Study Design: A prospective case-control study. Methodology: We included 78 pregnant females (39 hypertensive, and 39 normotensive; age range 18-39 years). Data concerning demographic and reproductive histories were recorded including previous pregnancies outcome and foetal complications. Comprehensive investigations of the current pregnancy including clinical examination and abdominal ultrasound scan were performed. Five ml venous blood was withdrawn from each female, processed and investigated for the presence of anti- Toxoplasma IgG antibodies by ELISA. Results: The overall Toxoplasma seroprevalence was 40/78 (51.3%) among total participants. Anti- T. gondii IgG antibodies were found in 23/39 (57.5%) of pregnancy-induced hypertension patients and in 17/39 (42.5%) normotensive controls (OR=1.85; 95% CI: 0.7-4.6; P =.17). Of the anti- T. gondii IgG positive patients, 19 (82.6%) had high IgG levels. In comparison only 1 (6.2%) of the anti- T. gondii IgG positive controls showed high IgG levels ( P <.0001). Regarding the specific characteristics of Toxoplasma positive pregnancy-induced hypertension patients, none of those characters displayed a significant correlation with hypertensive tendency except history of abortion ( P =.004). Conclusion: Chronic toxoplasmosis is not a likely risk factor for pregnancy-induced hypertension although significantly higher titre among hypertensive females necessitates further research.","PeriodicalId":9269,"journal":{"name":"British microbiology research journal","volume":"16 1","pages":"1-8"},"PeriodicalIF":0.0,"publicationDate":"2016-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88322158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Dynamics of Nitrogen on Soybean Field Amended with Poultry Manure 鸡粪改良大豆田氮素动态变化

British microbiology research journal Pub Date : 2016-01-10 DOI: 10.9734/BMRJ/2016/27759

M. Adigun, O. Babalola

引用次数: 1

Evaluation of Different Screening Methods for Biosurfactant Producers Isolated from Contaminated Egyptian Samples Grown on Industrial Olive Oil Processing Waste 从工业橄榄油加工废料中培养的埃及污染样品中分离生物表面活性剂不同筛选方法的评价

British microbiology research journal Pub Date : 2016-01-10 DOI: 10.9734/BMRJ/2016/28437

N. Sidkey, H. Mohamed, H. Elkhouly

引用次数: 20