Biotechnology Research International最新文献_第3页

Extracellular α-Galactosidase from Trichoderma sp. (WF-3): Optimization of Enzyme Production and Biochemical Characterization 木霉胞外α-半乳糖苷酶(WF-3):产酶优化及生化表征

Biotechnology Research International Pub Date : 2015-11-02 DOI: 10.1155/2015/860343

A. Chauhan, Arunesh Kumar, N. J. Siddiqi, Bechan Sharma

{"title":"Extracellular α-Galactosidase from Trichoderma sp. (WF-3): Optimization of Enzyme Production and Biochemical Characterization","authors":"A. Chauhan, Arunesh Kumar, N. J. Siddiqi, Bechan Sharma","doi":"10.1155/2015/860343","DOIUrl":"https://doi.org/10.1155/2015/860343","url":null,"abstract":"Trichoderma spp. have been reported earlier for their excellent capacity of secreting extracellular α-galactosidase. This communication focuses on the optimization of culture conditions for optimal production of enzyme and its characterization. The evaluation of the effects of different enzyme assay parameters such as stability, pH, temperature, substrate concentrations, and incubation time on enzyme activity has been made. The most suitable buffer for enzyme assay was found to be citrate phosphate buffer (50 mM, pH 6.0) for optimal enzyme activity. This enzyme was fairly stable at higher temperature as it exhibited 72% activity at 60°C. The enzyme when incubated at room temperature up to two hours did not show any significant loss in activity. It followed Michaelis-Menten curve and showed direct relationship with varying substrate concentrations. Higher substrate concentration was not inhibitory to enzyme activity. The apparent Michaelis-Menten constant (K m), maximum rate of reaction (V max), K cat, and catalytic efficiency values for this enzyme were calculated from the Lineweaver-Burk double reciprocal plot and were found to be 0.5 mM, 10 mM/s, 1.30 U mg−1, and 2.33 U mg−1 mM−1, respectively. This information would be helpful in understanding the biophysical and biochemical characteristics of extracellular α-galactosidase from other microbial sources.","PeriodicalId":9268,"journal":{"name":"Biotechnology Research International","volume":"29 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2015-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75245509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

In silico analysis of bioethanol overproduction by genetically modified microorganisms in coculture fermentation. 共培养发酵中转基因微生物生物乙醇生产过剩的计算机分析。

Biotechnology Research International Pub Date : 2015-01-01 Epub Date: 2015-02-16 DOI: 10.1155/2015/238082

Lisha K Parambil, Debasis Sarkar

{"title":"In silico analysis of bioethanol overproduction by genetically modified microorganisms in coculture fermentation.","authors":"Lisha K Parambil, Debasis Sarkar","doi":"10.1155/2015/238082","DOIUrl":"https://doi.org/10.1155/2015/238082","url":null,"abstract":"Lignocellulosic biomass is an attractive sustainable carbon source for fermentative production of bioethanol. In this context, use of microbial consortia consisting of substrate-selective microbes is advantageous as it eliminates the negative impacts of glucose catabolite repression. In this study, a detailed in silico analysis of bioethanol production from glucose-xylose mixtures of various compositions by coculture fermentation of xylose-selective Escherichia coli strain ZSC113 and glucose-selective wild-type Saccharomyces cerevisiae is presented. Dynamic flux balance models based on available genome-scale metabolic networks of the microorganisms have been used to analyze bioethanol production and the maximization of ethanol productivity is addressed by computing optimal aerobic-anaerobic switching times. A set of genetic engineering strategies for ethanol overproduction by E. coli strain ZSC113 have been evaluated for their efficiency in the context of batch coculture process. Finally, simulations are carried out to determine the pairs of genetically modified E. coli strain ZSC113 and S. cerevisiae that significantly enhance ethanol productivity in batch coculture fermentation.","PeriodicalId":9268,"journal":{"name":"Biotechnology Research International","volume":"2015 ","pages":"238082"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2015/238082","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33142107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

Application of two-stage cultivation for exploring the nutritional requirements for sporulation of three biocontrol fungi. 应用两阶段培养法探索三种生物防治真菌产孢的营养需求。

Biotechnology Research International Pub Date : 2015-01-01 Epub Date: 2015-01-29 DOI: 10.1155/2015/682839

Li Gao

引用次数: 2

Effect of Nitrite and Nitrate Concentrations on the Performance of AFB-MFC Enriched with High-Strength Synthetic Wastewater. 亚硝酸盐和硝酸盐浓度对高浓度合成废水富集AFB-MFC性能的影响

Biotechnology Research International Pub Date : 2015-01-01 Epub Date: 2015-10-01 DOI: 10.1155/2015/798397

Jian-Sheng Huang, Ping Yang, Chong-Ming Li, Yong Guo, Bo Lai, Ye Wang, Li Feng, Yun Zhang

{"title":"Effect of Nitrite and Nitrate Concentrations on the Performance of AFB-MFC Enriched with High-Strength Synthetic Wastewater.","authors":"Jian-Sheng Huang, Ping Yang, Chong-Ming Li, Yong Guo, Bo Lai, Ye Wang, Li Feng, Yun Zhang","doi":"10.1155/2015/798397","DOIUrl":"https://doi.org/10.1155/2015/798397","url":null,"abstract":"In order to study the effect of nitrite and nitrate on the performance of microbial fuel cell, a system combining an anaerobic fluidized bed (AFB) and a microbial fuel cell (MFC) was employed for high-strength nitrogen-containing synthetic wastewater treatment. Before this study, the AFB-MFC had been used to treat high-strength organic wastewater for about one year in a continuous flow mode. The results showed that when the concentrations of nitrite nitrogen and nitrate nitrogen were increased from 1700 mg/L to 4045 mg/L and 545 mg/L to 1427 mg/L, respectively, the nitrite nitrogen and nitrate nitrogen removal efficiencies were both above 99%; the COD removal efficiency went up from 60.00% to 88.95%; the voltage was about 375 ± 15 mV while the power density was at 70 ± 5 mW/m(2). However, when the concentrations of nitrite nitrogen and nitrate nitrogen were above 4045 mg/L and 1427 mg/L, respectively, the removal of nitrite nitrogen, nitrate nitrogen, COD, voltage, and power density were decreased to be 86%, 88%, 77%, 180 mV, and 17 mW/m(2) when nitrite nitrogen and nitrate nitrogen were increased to 4265 mg/L and 1661 mg/L. In addition, the composition of biogas generated in the anode chamber was analyzed by a gas chromatograph. Nitrogen gas, methane, and carbon dioxide were obtained. The results indicated that denitrification happened in anode chamber. ","PeriodicalId":9268,"journal":{"name":"Biotechnology Research International","volume":"2015 ","pages":"798397"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2015/798397","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34282844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Production and Multiplication of Native Compost Fungal Activator by Using Different Substrates and Its Influence on Growth and Development of Capsicum chinensis Jacq. "Bhut Jolokia". 使用不同基质生产和繁殖本地堆肥真菌活化剂及其对辣椒 "Bhut Jolokia "生长发育的影响。

Biotechnology Research International Pub Date : 2015-01-01 Epub Date: 2015-01-06 DOI: 10.1155/2015/481363

Vipin Parkash, Ankur Jyoti Saikia

{"title":"Production and Multiplication of Native Compost Fungal Activator by Using Different Substrates and Its Influence on Growth and Development of Capsicum chinensis Jacq. \"Bhut Jolokia\".","authors":"Vipin Parkash, Ankur Jyoti Saikia","doi":"10.1155/2015/481363","DOIUrl":"10.1155/2015/481363","url":null,"abstract":"In vitro experiment was carried out to see the effect of saw dusts of Pinus kesiya, Shorea robusta, and Callicarpa arborea on Trichoderma harzianum, isolate TH-13 mass production, along with its biotization effect on Capsicum chinensis Jacq. \"Bhut Jolokia.\" Early mycelium initiation (2 days) occurred in S. robusta followed by P. kesiya and C. arborea (3.5 days). The sporulation was observed earlier in S. robusta (100% after 6 days) than P. kesiya (33.4% after 8 days) and C. arborea (16.7% after 9 days) but no sporulation was observed in control. The complete sporulation was also earlier in S. robusta (100% after 10 days) than P. kesiya (33.4% after 15 days) and C. arborea (16.4% after 18 days). Higher conidial yield (86 × 10(6)) was also in S. robusta than P. kesiya (70 × 10(6)) and C. arborea (45 × 10(6)), respectively. The increase in height (60-70 cm), number of leaves (600-650), and yield of chili (120-150 fruits) were also more in inoculated C. chinensis seedlings than control. It is concluded that S. robusta saw dust is the best substrate for mass production of compost fungal activator and can be used in nursery practices for quality stock production of various crops/plantations. ","PeriodicalId":9268,"journal":{"name":"Biotechnology Research International","volume":"2015 ","pages":"481363"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4303005/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33012489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Establishment of MDCK Stable Cell Lines Expressing TMPRSS2 and MSPL and Their Applications in Propagating Influenza Vaccine Viruses in Absence of Exogenous Trypsin. 表达TMPRSS2和MSPL的MDCK稳定细胞系的建立及其在缺乏外源性胰蛋白酶的流感疫苗病毒繁殖中的应用

Biotechnology Research International Pub Date : 2015-01-01 Epub Date: 2015-03-30 DOI: 10.1155/2015/402628

Zhiyuan Wen, Chao Wu, Weiye Chen, Xianying Zeng, Jianzhong Shi, Jinying Ge, Hualan Chen, Zhigao Bu

{"title":"Establishment of MDCK Stable Cell Lines Expressing TMPRSS2 and MSPL and Their Applications in Propagating Influenza Vaccine Viruses in Absence of Exogenous Trypsin.","authors":"Zhiyuan Wen, Chao Wu, Weiye Chen, Xianying Zeng, Jianzhong Shi, Jinying Ge, Hualan Chen, Zhigao Bu","doi":"10.1155/2015/402628","DOIUrl":"https://doi.org/10.1155/2015/402628","url":null,"abstract":"We established two Madin-Darby canine kidney (MDCK) cell lines stably expressing human airway transmembrane protease: transmembrane protease, serine 2 (TMPRSS2) and mosaic serine protease large form (MSPL) which support multicycle growth of two H5 highly pathogenic avian influenza viruses (HPAIV) recombinant vaccines (Re-5 and Re-6) and an H9 avian influenza virus (AIV) recombinant vaccine (Re-9) in the absence of trypsin. Data showed that the cell lines stably expressed TMPRSS2 and MSPL after 20 serial passages. Both MDCK-TMPRSS2 and MDCK-MSPL could proteolytically cleave the HA of Re-5, Re-6, and Re-9 and supported high-titer growth of the vaccine without exogenous trypsin. Re-5, Re-6, and Re-9 efficiently infected and replicated within MDCK-TMPRSS2 and MDCK-MSPL cells and viral titer were comparable to the virus grown in MDCK cells with TPCK-trypsin. Thus, our results indicate a potential application for these cell lines in cell-based influenza vaccine production and may serve as a useful tool for HA proteolytic cleavage-related studies. ","PeriodicalId":9268,"journal":{"name":"Biotechnology Research International","volume":"2015 ","pages":"402628"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2015/402628","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33136218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Ethanol Production from Extruded Thermoplastic Maize Meal by High Gravity Fermentation with Zymomonas mobilis. 流动单胞菌高重力发酵挤出热塑性玉米粉生产乙醇。

Biotechnology Research International Pub Date : 2014-01-01 Epub Date: 2014-11-03 DOI: 10.1155/2014/654853

Mayeli Peralta-Contreras, Edna Aguilar-Zamarripa, Esther Pérez-Carrillo, Erandi Escamilla-García, Sergio Othon Serna-Saldívar

{"title":"Ethanol Production from Extruded Thermoplastic Maize Meal by High Gravity Fermentation with Zymomonas mobilis.","authors":"Mayeli Peralta-Contreras, Edna Aguilar-Zamarripa, Esther Pérez-Carrillo, Erandi Escamilla-García, Sergio Othon Serna-Saldívar","doi":"10.1155/2014/654853","DOIUrl":"https://doi.org/10.1155/2014/654853","url":null,"abstract":"A comparative study of extruded and ground maize meals as raw materials for the production of regular (12°P) and high gravity (20°P) worts was devised. Extruded water solubility index (WSI) was higher (9.8 percentage units) and crude fat was lower (2.64 percentage units) compared to ground maize. Free-amino nitrogen compounds (FAN), pH, and glucose were evaluated in regular and high gravity worts produced from ground or extruded maize. Extrusion improved glucose content and ethanol yield. In 20°P mashes, extrusion is enhanced by 2.14% initial glucose compared with regular ground mashes. The 12°P and 20°P extruded treatments averaged 12.2% and 8.4% higher ethanol, respectively, compared to the uncooked counterpart. The 20°P worts fermented with Zymomonas mobilis produced 9.56% more ethanol than the 12°P counterpart. The results show that the combination of extrusion and fermentation of 20°P worts improved ethanol yield per kg flour until 20.93%. This pretreatment stimulates Z. mobilis fermentation efficiency. ","PeriodicalId":9268,"journal":{"name":"Biotechnology Research International","volume":"2014 ","pages":"654853"},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2014/654853","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32927535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Evaluation of novel design strategies for developing zinc finger nucleases tools for treating human diseases. 评估用于开发治疗人类疾病的锌指核酸酶工具的新型设计策略。

Biotechnology Research International Pub Date : 2014-01-01 Epub Date: 2014-04-06 DOI: 10.1155/2014/970595

Christian Bach, William Sherman, Jani Pallis, Prabir Patra, Hassan Bajwa

引用次数: 0

Statistical Optimization of Media Components for Production of Fibrinolytic Alkaline Metalloproteases from Xenorhabdus indica KB-3. 印度Xenorhabdus indica KB-3产纤溶碱性金属蛋白酶培养基成分的统计优化

Biotechnology Research International Pub Date : 2014-01-01 Epub Date: 2014-04-23 DOI: 10.1155/2014/293434

Kumar Pranaw, Surender Singh, Debjani Dutta, Surabhi Chaudhuri, Sudershan Ganguly, Lata Nain

{"title":"Statistical Optimization of Media Components for Production of Fibrinolytic Alkaline Metalloproteases from Xenorhabdus indica KB-3.","authors":"Kumar Pranaw, Surender Singh, Debjani Dutta, Surabhi Chaudhuri, Sudershan Ganguly, Lata Nain","doi":"10.1155/2014/293434","DOIUrl":"https://doi.org/10.1155/2014/293434","url":null,"abstract":"Xenorhabdus indica KB-3, a well-known protease producer, was isolated from its entomopathogenic nematode symbiont Steinernema thermophilum. Since medium constituents are critical to the protease production, the chemical components of the selected medium (soya casein digest broth) were optimized by rotatable central composite design (RCCD) using response surface methodology (RSM). The effects of all five chemical components (considered as independent variables), namely tryptone, soya peptone, dextrose, NaCl, and dipotassium phosphate, on protease production (dependent variable) were studied, and it was found that tryptone and dextrose had maximum influence on protease production. The protease production was increased significantly by 66.31% under optimal medium conditions (tryptone-5.71, soya peptone-4.9, dextrose-1.45, NaCl-6.08, and dipotassium phosphate-0.47 in g/L). To best of knowledge, there are no reports on optimization of medium component for protease production by X. indica KB-3 using RSM and their application in fibrinolysis. This study will be useful for industrial processes for production of protease enzyme from X. indica KB-3 for its application in the field of agriculture and medicine. ","PeriodicalId":9268,"journal":{"name":"Biotechnology Research International","volume":"2014 ","pages":"293434"},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2014/293434","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32373103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

Biocontrol Activity of the Local Strain of Metschnikowia pulcherrima on Different Postharvest Pathogens. pulcherrima metschnikoia本地菌株对不同采后病原菌的生物防治活性研究。

Biotechnology Research International Pub Date : 2014-01-01 Epub Date: 2014-04-17 DOI: 10.1155/2014/397167

Sezai Türkel, Mihriban Korukluoğlu, Mümine Yavuz

{"title":"Biocontrol Activity of the Local Strain of Metschnikowia pulcherrima on Different Postharvest Pathogens.","authors":"Sezai Türkel, Mihriban Korukluoğlu, Mümine Yavuz","doi":"10.1155/2014/397167","DOIUrl":"https://doi.org/10.1155/2014/397167","url":null,"abstract":"The strains of the yeast Metschnikowia pulcherrima have strong biocontrol activity against various microorganisms. Biocontrol activity of M. pulcherrima largely depends on its iron immobilizing pigment pulcherrimin. Biocontrol activity of pulcherrimin producing strain, M. pulcherrima UMY15, isolated from local vineyards, was tested on different molds that cause food spoilage. M. pulcherrima UMY15 was a very effective biocontrol agent against Penicillium roqueforti, P. italicum, P. expansum, and Aspergillus oryzae in in-vitro plate tests. However, the inhibitory activity of M. pulcherrima UMY15 was less effective on Fusarium sp. and A. niger species in biocontrol assays. In addition, M. pulcherrima UMY15 strain completely inhibited the germination and mycelia growth of A. oryzae, A. parasiticus, and Fusarium sp. spores on artificial wounds of apples when they coinoculated with M. pulcherrima UMY15. Moreover, when coinoculated, M. pulcherrima UMY15 strain also inhibited the growth of P. roqueforti, P. italicum, P. expansum, A. oryzae, Fusarium sp., and Rhizopus sp. in grape juice, indicating that M. pulcherrima UMY15 can be used as a very effective biocontrol yeast against various species of postharvest pathogens, including Penicillium, Aspergillus, Fusarium, and Rhizopus. ","PeriodicalId":9268,"journal":{"name":"Biotechnology Research International","volume":"2014 ","pages":"397167"},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2014/397167","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32368204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 47