Biotechnology Research International最新文献_第2页

Synergistic Effects of Plant Growth Promoting Rhizobacteria and Chitosan on In Vitro Seeds Germination, Greenhouse Growth, and Nutrient Uptake of Maize (Zea mays L.) 促根菌和壳聚糖对玉米种子萌发、温室生长及养分吸收的协同效应

Biotechnology Research International Pub Date : 2016-01-20 DOI: 10.1155/2016/7830182

N. Agbodjato, Pacôme A. Noumavo, A. Adjanohoun, Léonce Agbessi, L. Baba-Moussa

{"title":"Synergistic Effects of Plant Growth Promoting Rhizobacteria and Chitosan on In Vitro Seeds Germination, Greenhouse Growth, and Nutrient Uptake of Maize (Zea mays L.)","authors":"N. Agbodjato, Pacôme A. Noumavo, A. Adjanohoun, Léonce Agbessi, L. Baba-Moussa","doi":"10.1155/2016/7830182","DOIUrl":"https://doi.org/10.1155/2016/7830182","url":null,"abstract":"This study aimed to assess the effects of three plant growth promoting rhizobacteria (PGPR) and chitosan either singly or in combination on maize seeds germination and growth and nutrient uptake. Maize seeds were treated with chitosan and bacterial solution. The germination and growth tests were carried out in square Petri dishes and plastic pots. The combination chitosan-A. lipoferum-P. fluorescens has increased the seeds vigor index up to 36.44% compared to the control. In comparison to the control, P. putida has significantly improved root weight (44.84%) and germinated seed weight (31.39%) whereas chitosan-P. putida has increased the shoot weight (65.67%). For the growth test, the maximal heights (17.66%) were obtained by plants treated with the combination A. lipoferum-P. fluorescens-P. putida. Chitosan-P. fluorescens induced the highest increases of leaves per plant (50.09%), aerial (84.66%), and underground biomass (108.77%) production. The plants inoculated with A. lipoferum had the large leaf areas with an increase of 54.08%, while combinations P. fluorescens-P. putida and chitosan-A. lipoferum improved the aerial and underground dry matter of plants to 26.35% and 18.18%. The nitrogen content of the plants was increased by chitosan-A. lipoferum-P. fluorescens-P. putida with an increasing of 41.61%. The combination of chitosan and PGPR can be used as biological fertilizers to increase maize production.","PeriodicalId":9268,"journal":{"name":"Biotechnology Research International","volume":"114 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2016-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88063801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 72

The Use of Adenovirus Dodecahedron in the Delivery of an Enzymatic Activity in the Cell. 利用腺病毒十二面体在细胞内传递酶活性。

Biotechnology Research International Pub Date : 2016-01-01 Epub Date: 2016-05-08 DOI: 10.1155/2016/5030589

Sumarheni, Benoit Gallet, Pascal Fender

{"title":"The Use of Adenovirus Dodecahedron in the Delivery of an Enzymatic Activity in the Cell.","authors":"Sumarheni, Benoit Gallet, Pascal Fender","doi":"10.1155/2016/5030589","DOIUrl":"https://doi.org/10.1155/2016/5030589","url":null,"abstract":"<p><p>Penton-dodecahedron (Pt-Dd) derived from adenovirus type 3 is a symmetric complex of pentameric penton base plus fiber which can be produced in the baculovirus system at a high concentration. The size of Pt-Dd is smaller than the virus, but this virus-like particle (VLP) has the major proteins recognized by specific receptors on the surface of almost all types of cell. In this study, by direct observation with fluorescence microscopy on a fixed and living cell, the intracellular trafficking and localization of Pt-Dd labeled with fluorescence dyes in the cytoplasm of HeLa Tub-GFP showed a rapid internalization characteristic. Subsequently, the linkage of horseradish peroxidase (HRP) with Pt-Dd as the vector demonstrated an efficient system to deliver this enzyme into the cell without interfering its enzymatic activity as shown by biochemical and cellular experiments. These results were supported by additional studies using Bs-Dd or free form of the HRP used as the control. Overall, this study strengthens the potential role of Pt-Dd as an alternative vector for delivering therapeutic agents. </p>","PeriodicalId":9268,"journal":{"name":"Biotechnology Research International","volume":"2016 ","pages":"5030589"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2016/5030589","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34533406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Role of Heat-Shock Proteins in Cellular Function and in the Biology of Fungi 热休克蛋白在真菌细胞功能和生物学中的作用

Biotechnology Research International Pub Date : 2015-12-31 DOI: 10.1155/2015/132635

S. Tiwari, R. Thakur, J. Shankar

{"title":"Role of Heat-Shock Proteins in Cellular Function and in the Biology of Fungi","authors":"S. Tiwari, R. Thakur, J. Shankar","doi":"10.1155/2015/132635","DOIUrl":"https://doi.org/10.1155/2015/132635","url":null,"abstract":"Stress (biotic or abiotic) is an unfavourable condition for an organism including fungus. To overcome stress, organism expresses heat-shock proteins (Hsps) or chaperons to perform biological function. Hsps are involved in various routine biological processes such as transcription, translation and posttranslational modifications, protein folding, and aggregation and disaggregation of proteins. Thus, it is important to understand holistic role of Hsps in response to stress and other biological conditions in fungi. Hsp104, Hsp70, and Hsp40 are found predominant in replication and Hsp90 is found in transcriptional and posttranscriptional process. Hsp90 and Hsp70 in combination or alone play a major role in morphogenesis and dimorphism. Heat stress in fungi expresses Hsp60, Hsp90, Hsp104, Hsp30, and Hsp10 proteins, whereas expression of Hsp12 protein was observed in response to cold stress. Hsp30, Hsp70, and Hsp90 proteins showed expression in response to pH stress. Osmotic stress is controlled by small heat-shock proteins and Hsp60. Expression of Hsp104 is observed under high pressure conditions. Out of these heat-shock proteins, Hsp90 has been predicted as a potential antifungal target due to its role in morphogenesis. Thus, current review focuses on role of Hsps in fungi during morphogenesis and various stress conditions (temperature, pH, and osmotic pressure) and in antifungal drug tolerance.","PeriodicalId":9268,"journal":{"name":"Biotechnology Research International","volume":"64 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2015-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88849580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 158

Prospects for Irradiation in Cellulosic Ethanol Production 辐照在纤维素乙醇生产中的应用前景

Biotechnology Research International Pub Date : 2015-12-29 DOI: 10.1155/2015/157139

A. Saini, N. Aggarwal, Anuja Sharma, A. Yadav

引用次数: 40

Response Surface Optimization of Bioethanol Production from Sugarcane Molasses by Pichia veronae Strain HSC-22 毕赤酵母HSC-22产糖乙醇的响应面优化

Biotechnology Research International Pub Date : 2015-12-08 DOI: 10.1155/2015/905792

H. Hamouda, H. Nassar, H. Madian, S. A. Abu Amr, N. S. El-Gendy

引用次数: 22

Enhanced Bioremediation of Soil Artificially Contaminated with Petroleum Hydrocarbons after Amendment with Capra aegagrus hircus (Goat) Manure 山羊粪便对石油烃人工污染土壤的生物修复作用

Biotechnology Research International Pub Date : 2015-12-06 DOI: 10.1155/2015/657349

T. Nwogu, C. C. Azubuike, C. Ogugbue

{"title":"Enhanced Bioremediation of Soil Artificially Contaminated with Petroleum Hydrocarbons after Amendment with Capra aegagrus hircus (Goat) Manure","authors":"T. Nwogu, C. C. Azubuike, C. Ogugbue","doi":"10.1155/2015/657349","DOIUrl":"https://doi.org/10.1155/2015/657349","url":null,"abstract":"This study was carried out to evaluate the biostimulant potentials of Capra aegagrus hircus manure for bioremediation of crude oil contaminated soil (COCS) under tropical conditions. 1 kg of COCS sample was amended with 0.02 kg of C. a. hircus manure and monitored at 14-day intervals for total petroleum hydrocarbon (TPH), nutrient content, and changes in microbial counts. At the end of the study period, there was 62.08% decrease in the concentration of TPH in the amended sample compared to 8.15% decrease in the unamended sample, with significant differences (P < 0.05) in TPH concentrations for both samples at different time intervals. Similarly, there was a gradual decrease in the concentrations of total organic carbon, nitrogen, phosphorus, and potassium in both samples. The culturable hydrocarbon-utilizing bacteria (CHUB) increased steadily from 8.5 × 105 cfu/g to 2.70 × 106 cfu/g and from 8.0 × 105 cfu/g to 1.78 × 106 cfu/g for both samples. Acinetobacter, Achromobacter, Bacillus, Flavobacterium, Klebsiella, Micrococcus, Pseudomonas, and Staphylococcus were isolated from amended sample with Pseudomonas being the predominant isolated bacterial genus. This study demonstrated that C. a. hircus manure is a good biostimulant, which enhanced the activities of indigenous hydrocarbonoclastic bacteria resulting in significant decrease in TPH concentration of COCS.","PeriodicalId":9268,"journal":{"name":"Biotechnology Research International","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2015-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89660467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 47

Plant Growth Promotion Activity of Keratinolytic Fungi Growing on a Recalcitrant Waste Known as “Hair Waste” 生长在顽固性废物“毛发废物”上的角朊酶真菌促进植物生长的活性

Biotechnology Research International Pub Date : 2015-11-30 DOI: 10.1155/2015/952921

I. Cavello, J. M. Crespo, S. S. García, José M. Zapiola, M. F. Luna, S. Cavalitto

{"title":"Plant Growth Promotion Activity of Keratinolytic Fungi Growing on a Recalcitrant Waste Known as “Hair Waste”","authors":"I. Cavello, J. M. Crespo, S. S. García, José M. Zapiola, M. F. Luna, S. Cavalitto","doi":"10.1155/2015/952921","DOIUrl":"https://doi.org/10.1155/2015/952921","url":null,"abstract":"Purpureocillium lilacinum (Thom) Samsom is one of the most studied fungi in the control of plant parasitic nematodes. However, there is not specific information on its ability to inhibit some pathogenic bacteria, fungi, or yeast. This work reports the production of several antifungal hydrolytic enzymes by a strain of P. lilacinum when it is grown in a medium containing hair waste. The growth of several plant-pathogenic fungi, Alternaria alternata, Aspergillus niger, and Fusarium culmorum, was considerably affected by the presence of P. lilacinum's supernatant. Besides antifungal activity, P. lilacinum demonstrates the capability to produce indoleacetic acid and ammonia during time cultivation on hair waste medium. Plant growth-promoting activity by cell-free supernatant was evidenced through the increase of the percentage of tomato seed germination from 71 to 85% after 48 hours. A 21-day plant growth assay using tomato plants indicates that crude supernatant promotes the growth of the plants similar to a reference fertilizer (p > 0.05). These results suggest that both strain and the supernatant may have potential to be considered as a potent biocontrol agent with multiple plant growth-promoting properties. To our knowledge, this is the first report on the antifungal, IAA production and tomato growth enhancing compounds produced by P. lilacinum LPSC #876.","PeriodicalId":9268,"journal":{"name":"Biotechnology Research International","volume":"30 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2015-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81429506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 30

Production of Oxidative and Hydrolytic Enzymes by Coprinus cinereus (Schaeff.) Gray from Sisal Wastes Supplemented with Cow Dung Manure 红毛鸡生产氧化和水解酶的研究由剑麻废料和牛粪补充而成的灰色

Biotechnology Research International Pub Date : 2015-11-19 DOI: 10.1155/2015/650543

P. Raymond, A. Mshandete, Amelia Kajumulo Kivaisi

{"title":"Production of Oxidative and Hydrolytic Enzymes by Coprinus cinereus (Schaeff.) Gray from Sisal Wastes Supplemented with Cow Dung Manure","authors":"P. Raymond, A. Mshandete, Amelia Kajumulo Kivaisi","doi":"10.1155/2015/650543","DOIUrl":"https://doi.org/10.1155/2015/650543","url":null,"abstract":"The activity of oxidative and hydrolytic enzymes of the edible and medicinal white rot fungi Coprinus cinereus (Schaeff.) Gray mushroom was observed during mycelia growth and fruiting body development in solid substrate fermentation using sisal waste fractions amended with cow dung manure as supplement. Laccase had the highest titre value among the five detected enzymes. Its activity was higher during mycelia growth compared to fruiting phase, with 10% supplemented substrate formulation unmixed sisal leaf decortication residues [abbreviated SL : SB (100 : 0)] displaying the highest activity of 39.45 ± 12.05 Ug−1. Lignin peroxidase (LiP) exhibited a characteristic wave-like pattern with the highest peaks found either during full mycelia colonization or soon after first flush harvest; the highest activity of 1.93 ± 0.62 Ug−1 was observed on unsupplemented SL : SB (100 : 0) substrate formulation during mycelia colonization. For hydrolytic enzymes, the highest carboxymethyl cellulase (CMCase) activity of 2.03 ± 0.70 Ug−1 was observed on 20% supplemented SL : SB (0 : 100) after first flush; that of pectinase (1.90 ± 0.32 Ug−1) was revealed after third flush on 10% supplemented SL : SB (0 : 100) substrate formulation while 10% supplemented SL : SB (25 : 75) exhibited the highest xylanase activity (1.23 ± 0.12 Ug−1) after first flush. These findings show that the activities of both oxidative and hydrolytic enzymes were regulated in line with developmental phase of growth of Coprinus cinereus.","PeriodicalId":9268,"journal":{"name":"Biotechnology Research International","volume":"16 3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2015-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81045900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Technical and Socioeconomic Potential of Biogas from Cassava Waste in Ghana 加纳木薯废料沼气的技术和社会经济潜力

Biotechnology Research International Pub Date : 2015-11-18 DOI: 10.1155/2015/828576

F. Kemausuor, A. Addo, Lawrence Darkwah

{"title":"Technical and Socioeconomic Potential of Biogas from Cassava Waste in Ghana","authors":"F. Kemausuor, A. Addo, Lawrence Darkwah","doi":"10.1155/2015/828576","DOIUrl":"https://doi.org/10.1155/2015/828576","url":null,"abstract":"This study analyses technical potential and ex ante socioeconomic impacts of biogas production using cassava waste from agroprocessing plants. An analysis was performed for two biodigesters in two cassava processing communities in Ghana. The results showed that the two communities generate an excess of 4,500 tonnes of cassava peels per year. Using approximately 5% of the peels generated and livestock manure as inoculum can generate approximately 75,000 m3 of gas with an estimated 60% methane content from two separate plants of capacities 500 m3 and 300 m3 in the two communities. If used internally as process fuel, the potential gas available could replace over 300 tonnes of firewood per year for cassava processing. The displacement of firewood with gas could have environmental, economic, and social benefits in creating sustainable development. With a 10 percent discount rate, an assumed 20-year biodigester will have a Net Present Value of approximately US$ 148,000, 7-year Payback Period, and an Internal Rate of Return of 18.7%. The project will create 10 full-time unskilled labour positions during the investment year and 4 positions during operation years.","PeriodicalId":9268,"journal":{"name":"Biotechnology Research International","volume":"42 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2015-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82526953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 22

Metagenomics: Retrospect and Prospects in High Throughput Age 宏基因组学:高通量时代的回顾与展望

Biotechnology Research International Pub Date : 2015-11-17 DOI: 10.1155/2015/121735

Satish Kumar, K. Krishnani, B. Bhushan, M. Brahmane

引用次数: 47