Biotechnology Research International最新文献_第10页

Optimization for the production of cellulase enzyme from municipal solid waste residue by two novel cellulolytic fungi. 两种新型纤维素水解真菌以城市生活垃圾为原料生产纤维素酶的优化研究。

Biotechnology Research International Pub Date : 2011-01-23 DOI: 10.4061/2011/810425

S P Gautam, P S Bundela, A K Pandey, Jamaluddin Khan, M K Awasthi, S Sarsaiya

引用次数: 214

PCR-Based Identification and Characterization of Fusarium sp. Associated with Mango Malformation. 基于pcr的芒果畸形镰刀菌鉴定与特性研究。

Biotechnology Research International Pub Date : 2011-01-20 DOI: 10.4061/2011/141649

M Arif, D R Pani, N W Zaidi, U S Singh

{"title":"PCR-Based Identification and Characterization of Fusarium sp. Associated with Mango Malformation.","authors":"M Arif, D R Pani, N W Zaidi, U S Singh","doi":"10.4061/2011/141649","DOIUrl":"https://doi.org/10.4061/2011/141649","url":null,"abstract":"Mango malformation is the most serious disease of mango causing considerable damage to the mango orchards worldwide. It is a major threat for mango cultivation in north Indian belt. In recent years, Fusarium sp. is finding wide acceptability in scientific community as a causal agent of this disease. However, little information is known about the variability in Fusarium isolates from malformed mango tissues. Therefore, the major objective of present study was the identification and analysis of genetic diversity among Fusarium isolates collected from malformed mango tissues. Two texon selective primers, ITS-Fu-f and ITS-Fu-r, were used for quick identification of Fusarium spp. The fungal genomic DNA was extracted from using CTAB method and was utilized as template for PCR amplification. Total 224 bands were amplified by 18 RAPD primers at an average of 12.44 bands per primer. The size of the obtained amplicons ranged from 0.264 kb (minimum) to 3.624 kb (maximum). Data scored from 25 isolates of Fusarium sp. with 18 RAPD primers were used to generate similarity coefficients. The similarity coefficient ranged from 0.17 to 0.945. Based on DNA fingerprints, all isolates were categorized into two major clusters. This study indicated a wide variability among different isolates of Fusarium.","PeriodicalId":9268,"journal":{"name":"Biotechnology Research International","volume":"2011 ","pages":"141649"},"PeriodicalIF":0.0,"publicationDate":"2011-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4061/2011/141649","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29696712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 18

Generation of HIV-1 and Internal Control Transcripts as Standards for an In-House Quantitative Competitive RT-PCR Assay to Determine HIV-1 Viral Load. 生成HIV-1和内部控制转录本作为内部定量竞争性RT-PCR测定HIV-1病毒载量的标准。

Biotechnology Research International Pub Date : 2011-01-01 Epub Date: 2011-06-23 DOI: 10.4061/2011/964831

Anny Armas Cayarga, Yenitse Perea Hernández, Yaimé J González González, Santiago Dueñas Carrera, Idania González Pérez, René Robaina Álvarez

{"title":"Generation of HIV-1 and Internal Control Transcripts as Standards for an In-House Quantitative Competitive RT-PCR Assay to Determine HIV-1 Viral Load.","authors":"Anny Armas Cayarga, Yenitse Perea Hernández, Yaimé J González González, Santiago Dueñas Carrera, Idania González Pérez, René Robaina Álvarez","doi":"10.4061/2011/964831","DOIUrl":"https://doi.org/10.4061/2011/964831","url":null,"abstract":"Human immunodeficiency virus type-1 (HIV-1) viral load is useful for monitoring disease progression in HIV-infected individuals. We generated RNA standards of HIV-1 and internal control (IC) by in vitro transcription and evaluated its performance in a quantitative reverse transcription polymerase chain reaction (qRT-PCR) assay. HIV-1 and IC standards were obtained at high RNA concentrations, without DNA contamination. When these transcripts were included as standards in a qRT-PCR assay, it was obtained a good accuracy (±0.5 log(10) unit of the expected results) in the quantification of the HIV-1 RNA international standard and controls. The lower limit detection achieved using these standards was 511.0 IU/mL. A high correlation (r = 0.925) was obtained between the in-house qRT-PCR assay and the NucliSens easyQ HIV-1 test (bioMerieux) for HIV-1 RNA quantitation with clinical samples (N = 14). HIV-1 and IC RNA transcripts, generated in this study, proved to be useful as standards in an in-house qRT-PCR assay for determination of HIV-1 viral load.","PeriodicalId":9268,"journal":{"name":"Biotechnology Research International","volume":"2011 ","pages":"964831"},"PeriodicalIF":0.0,"publicationDate":"2011-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4061/2011/964831","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30011667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

In Vitro Antimicrobial, Antioxidant Activities and Phytochemical Analysis of Canarium patentinervium Miq. from Malaysia. 菜豆的体外抗菌、抗氧化活性及植物化学分析。从马来西亚。

Biotechnology Research International Pub Date : 2011-01-01 Epub Date: 2011-06-23 DOI: 10.4061/2011/768673

R Mogana, Khoo Teng-Jin, C Wiart

引用次数: 27

Direct spectrophotometric assay for benzaldehyde lyase activity. 苯甲醛裂解酶活性的直接分光光度法测定。

Biotechnology Research International Pub Date : 2011-01-01 Epub Date: 2011-07-14 DOI: 10.4061/2011/478925

Dessy Natalia, Christina Kohlmann, Marion B Ansorge-Schumacher, Lasse Greiner

引用次数: 3

Human coronary artery smooth muscle cell responses to bioactive polyelectrolyte multilayer interfaces. 人冠状动脉平滑肌细胞对生物活性聚电解质多层界面的反应。

Biotechnology Research International Pub Date : 2011-01-01 Epub Date: 2010-11-29 DOI: 10.4061/2011/854068

Robert G Newcomer, Maroun D Moussallem, Thomas C S Keller, Joseph B Schlenoff, Qing-Xiang Amy Sang

{"title":"Human coronary artery smooth muscle cell responses to bioactive polyelectrolyte multilayer interfaces.","authors":"Robert G Newcomer, Maroun D Moussallem, Thomas C S Keller, Joseph B Schlenoff, Qing-Xiang Amy Sang","doi":"10.4061/2011/854068","DOIUrl":"https://doi.org/10.4061/2011/854068","url":null,"abstract":"Under normal physiological conditions, mature human coronary artery smooth muscle cells (hCASMCs) exhibit a \"contractile\" phenotype marked by low rates of proliferation and protein synthesis, but these cells possess the remarkable ability to dedifferentiate into a \"synthetic\" phenotype when stimulated by conditions of pathologic stress. A variety of polyelectrolyte multilayer (PEMU) films are shown here to exhibit bioactive properties that induce distinct responses from cultured hCASMCs. Surfaces terminated with Nafion or poly(styrenesulfonic acid) (PSS) induce changes in the expression and organization of intracellular proteins, while a hydrophilic, zwitterionic copolymer of acrylic acid and 3-[2-(acrylamido)-ethyl dimethylammonio] propane sulfonate (PAA-co-PAEDAPS) is resistant to cell attachment and suppresses the formation of key cytoskeletal components. Differential expression of heat shock protein 90 and actin is observed, in terms of both their magnitude and cellular localization, and distinct cytoplasmic patterns of vimentin are seen. The ionophore A23187 induces contraction in confluent hCASMC cultures on Nafion-terminated surfaces. These results demonstrate that PEMU coatings exert direct effects on the cytoskeletal organization of attaching hCASMCs, impeding growth in some cases, inducing changes consistent with phenotypic modulation in others, and suggesting potential utility for PEMU surfaces as a coating for coronary artery stents and other implantable medical devices.","PeriodicalId":9268,"journal":{"name":"Biotechnology Research International","volume":"2011 ","pages":"854068"},"PeriodicalIF":0.0,"publicationDate":"2011-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4061/2011/854068","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29696251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

The use of endophytes to obtain bioactive compounds and their application in biotransformation process. 内生菌获取生物活性化合物及其在生物转化过程中的应用。

Biotechnology Research International Pub Date : 2011-01-01 Epub Date: 2010-09-26 DOI: 10.4061/2011/576286

Mariana Recco Pimentel, Gustavo Molina, Ana Paula Dionísio, Mário Roberto Maróstica Junior, Gláucia Maria Pastore

引用次数: 234

Microbial degradation of petroleum hydrocarbon contaminants: an overview. 石油烃污染物的微生物降解：概述。

Biotechnology Research International Pub Date : 2011-01-01 Epub Date: 2010-09-13 DOI: 10.4061/2011/941810

Nilanjana Das, Preethy Chandran

{"title":"Microbial degradation of petroleum hydrocarbon contaminants: an overview.","authors":"Nilanjana Das, Preethy Chandran","doi":"10.4061/2011/941810","DOIUrl":"10.4061/2011/941810","url":null,"abstract":"One of the major environmental problems today is hydrocarbon contamination resulting from the activities related to the petrochemical industry. Accidental releases of petroleum products are of particular concern in the environment. Hydrocarbon components have been known to belong to the family of carcinogens and neurotoxic organic pollutants. Currently accepted disposal methods of incineration or burial insecure landfills can become prohibitively expensive when amounts of contaminants are large. Mechanical and chemical methods generally used to remove hydrocarbons from contaminated sites have limited effectiveness and can be expensive. Bioremediation is the promising technology for the treatment of these contaminated sites since it is cost-effective and will lead to complete mineralization. Bioremediation functions basically on biodegradation, which may refer to complete mineralization of organic contaminants into carbon dioxide, water, inorganic compounds, and cell protein or transformation of complex organic contaminants to other simpler organic compounds by biological agents like microorganisms. Many indigenous microorganisms in water and soil are capable of degrading hydrocarbon contaminants. This paper presents an updated overview of petroleum hydrocarbon degradation by microorganisms under different ecosystems.","PeriodicalId":9268,"journal":{"name":"Biotechnology Research International","volume":"2011 ","pages":"941810"},"PeriodicalIF":0.0,"publicationDate":"2011-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3042690/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"29696254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Statistical Approach for Optimization of Simultaneous Production of β-Glucosidase and Endoglucanase by Rhizopus oryzae from Solid-State Fermentation of Water Hyacinth Using Central Composite Design. 利用中心复合设计优化风信子固态发酵根瘤菌同时生产β-葡萄糖苷酶和内切葡聚糖酶的统计方法

Biotechnology Research International Pub Date : 2011-01-01 Epub Date: 2011-05-30 DOI: 10.4061/2011/574983

Moumita Karmakar, Rina Rani Ray

{"title":"A Statistical Approach for Optimization of Simultaneous Production of β-Glucosidase and Endoglucanase by Rhizopus oryzae from Solid-State Fermentation of Water Hyacinth Using Central Composite Design.","authors":"Moumita Karmakar, Rina Rani Ray","doi":"10.4061/2011/574983","DOIUrl":"10.4061/2011/574983","url":null,"abstract":"The production cost of β-glucosidase and endoglucanase could be reduced by using water hyacinth, an aquatic weed, as the sole carbon source and using cost-efficient fermentation strategies like solid-state fermentation (SSF). In the present study, the effect of different production conditions on the yield of β-glucosidase and endoglucanase by Rhizopus oryzae MTCC 9642 from water hyacinth was investigated systematically using response surface methodology. A Central composite experimental design was applied to optimize the impact of three variables, namely, substrate concentration, pH, and temperature, on enzyme production. The optimal level of each parameter for maximum enzyme production by the fungus was determined. Highest activity of endoglucanase of 495 U/mL was achieved at a substrate concentration of 1.23%, pH 7.29, and temperature 29.93°C whereas maximum β-glucosidase activity of 137.32 U/ml was achieved at a substrate concentration of 1.25%, pH 6.66, and temperature 32.09°C. There was a direct correlation between the levels of enzymatic activities and the substrate concentration of water hyacinth as carbon source.","PeriodicalId":9268,"journal":{"name":"Biotechnology Research International","volume":"2011 ","pages":"574983"},"PeriodicalIF":0.0,"publicationDate":"2011-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3113439/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30252115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Somatic embryogenesis and massive shoot regeneration from immature embryo explants of tef. tef未成熟胚外植体的体细胞胚胎发生和大量芽再生。

Biotechnology Research International Pub Date : 2011-01-01 Epub Date: 2011-10-18 DOI: 10.4061/2011/309731

Likyelesh Gugsa, Jochen Kumlehn

{"title":"Somatic embryogenesis and massive shoot regeneration from immature embryo explants of tef.","authors":"Likyelesh Gugsa, Jochen Kumlehn","doi":"10.4061/2011/309731","DOIUrl":"https://doi.org/10.4061/2011/309731","url":null,"abstract":"Tef (Eragrostis tef) provides a major source of human nutrition in the Horn of Africa, but biotechnology has had little impact on its improvement to date. Here, we report the elaboration of an in vitro regeneration protocol, based on the use of immature zygotic embryos as explant. Explant size was an important determinant of in vitro regeneration efficiency, as was the formulation of the culture medium. Optimal results were obtained by culturing 0.2-0.35 mm embryo explants on a medium containing KBP minerals, 9.2-13.8 μM 2,4-dichlorophenoxyacetic acid, 6 mM glutamine, and 0.5% Phytagel. Although this protocol was effective for both the improved cultivar \"DZ-01-196\" and the landrace \"Fesho\", the former produced consistently more embryogenic tissue and a higher number of regenerants. An average of more than 2,800 shoots could be obtained from each \"DZ-01-196\" explant after 12 weeks of in vitro culture. These shoots readily formed roots, and plantlets transferred to soil were able to develop into morphologically normal, fertile plants. This regeneration and multiplication system should allow for the application of a range of biotechnological methods to tef.","PeriodicalId":9268,"journal":{"name":"Biotechnology Research International","volume":"2011 ","pages":"309731"},"PeriodicalIF":0.0,"publicationDate":"2011-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4061/2011/309731","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30235193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 15