Optimization for the production of cellulase enzyme from municipal solid waste residue by two novel cellulolytic fungi.

S P Gautam, P S Bundela, A K Pandey, Jamaluddin Khan, M K Awasthi, S Sarsaiya
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引用次数: 214

Abstract

The main purpose of this study is to reduce the production cost of cellulase by optimizing the production medium and using an alternative carbon source such as municipal solid waste residue. In the present investigation, we aim to isolate the two novel cellulase producing fungi (Aspergillus niger and Trichoderma sp.) from municipal solid waste. Municipal solid waste residue (4-5% (w/v)) and peptone and yeast extract (1.0% (w/v)) were found to be the best combination of carbon and nitrogen sources for the production of cellulase by A. niger and Trichoderma sp. Optimum temperature and pH of the medium for the cellulase production by A. niger were 40°C and 6-7, whereas those for the production of cellulase by Trichoderma sp. were 45°C and 6.5. Cellulase production from A. niger and Trichoderma sp. can be an advantage as the enzyme production rate is normally higher as compared to other fungi.

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两种新型纤维素水解真菌以城市生活垃圾为原料生产纤维素酶的优化研究。
本研究的主要目的是通过优化生产介质和使用城市固体废弃物等替代碳源来降低纤维素酶的生产成本。在本研究中,我们旨在从城市生活垃圾中分离出两种新的产纤维素酶真菌(黑曲霉和木霉)。城市生活垃圾残渣(4-5% (w/v))和蛋白胨和酵母提取物(1.0% (w/v))是黑曲霉和木霉生产纤维素酶的最佳碳氮源组合。黑曲霉生产纤维素酶的最佳培养基温度和pH分别为40℃和6-7,木霉生产纤维素酶的最佳培养基温度和pH分别为45℃和6.5。黑曲霉和木霉生产纤维素酶可能是一个优势,因为与其他真菌相比,酶的生产速度通常更高。
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