Bioengineering & Translational Medicine最新文献_第9页

Unleashing the potential of mRNA: Overcoming delivery challenges with nanoparticles 释放 mRNA 的潜力：利用纳米颗粒克服递送难题

IF 6.1 2区医学

Bioengineering & Translational Medicine Pub Date : 2024-08-15 DOI: 10.1002/btm2.10713

Qiang Chen, Ku-Geng Huo, Sheng-Min Ji, Shu-De Pang, Tian-Ying Sun, Yi Niu, Zi-Hao Jiang, Peng Zhang, Shu-Xin Han, Jin-Yao Li

{"title":"Unleashing the potential of mRNA: Overcoming delivery challenges with nanoparticles","authors":"Qiang Chen, Ku-Geng Huo, Sheng-Min Ji, Shu-De Pang, Tian-Ying Sun, Yi Niu, Zi-Hao Jiang, Peng Zhang, Shu-Xin Han, Jin-Yao Li","doi":"10.1002/btm2.10713","DOIUrl":"10.1002/btm2.10713","url":null,"abstract":"Messenger RNA (mRNA) has emerged as a promising therapeutic strategy for various diseases, including cancer, infectious diseases, and genetic disorders. The mRNA-based therapeutics have gained significant attention due to their ability to regulate targeted cells, activate immune cells, and avoid potential risks associated with DNA-based technology. However, the clinical application of mRNA in cancer therapy is hindered by the instability of RNA, physiological barriers, and the risk of immunogenic hurdles. To overcome these challenges and ensure the safe delivery of mRNA therapeutics to target sites, nanoparticle-based delivery systems have been explored as potential tools in vitro and in vivo applications. This review provides a comprehensive overview of the current status of mRNA therapy, discussing its advantages and limitations, delivery strategies and materials, as well as applications in different fields. By exploring these aspects, the researcher can gain a more complete understanding of the current state, prospects, and challenges of mRNA technologies.","PeriodicalId":9263,"journal":{"name":"Bioengineering & Translational Medicine","volume":"10 2","pages":""},"PeriodicalIF":6.1,"publicationDate":"2024-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/btm2.10713","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141991784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to “Activation of NR1H3 attenuates the severity of septic myocardial injury by inhibiting NLRP3 inflammasome” 对 "通过抑制 NLRP3 炎症小体激活 NR1H3 减轻脓毒症心肌损伤的严重程度 "的更正

IF 6.1 2区医学

Bioengineering & Translational Medicine Pub Date : 2024-08-15 DOI: 10.1002/btm2.10707

引用次数: 0

MHCI trafficking signal-based mRNA vaccines strengthening immune protection against RNA viruses 基于 MHCI 转运信号的 mRNA 疫苗可加强对 RNA 病毒的免疫保护

IF 6.1 2区医学

Bioengineering & Translational Medicine Pub Date : 2024-08-15 DOI: 10.1002/btm2.10709

Yupei Zhang, Songhui Zhai, Shugang Qin, Yuting Chen, Kepan Chen, Zhiying Huang, Xing Lan, Yaoyao Luo, Guohong Li, Hao Li, Xi He, Meiwan Chen, Zhongwei Zhang, Xingchen Peng, Xin Jiang, Hai Huang, Xiangrong Song

引用次数: 0

Activated neutrophils: A next generation cellular immunotherapy 活化的中性粒细胞新一代细胞免疫疗法

IF 6.1 2区医学

Bioengineering & Translational Medicine Pub Date : 2024-08-13 DOI: 10.1002/btm2.10704

Ninad Kumbhojkar, Samir Mitragotri

{"title":"Activated neutrophils: A next generation cellular immunotherapy","authors":"Ninad Kumbhojkar, Samir Mitragotri","doi":"10.1002/btm2.10704","DOIUrl":"10.1002/btm2.10704","url":null,"abstract":"Cell therapies are at the forefront of novel therapeutics. Neutrophils, despite being the most populous immune cells in human blood circulation, are not considered a viable option for cellular therapies because of their short lifespan and poor understanding of their role in the pathophysiology of various diseases. In inflammatory conditions, neutrophils exhibit an activated phenotype. Activation brings about significant changes to neutrophil biology such as increased lifespan, inflammatory cytokine secretion, and enhanced effector functions. Activated neutrophils also possess the potential to stimulate the downstream immune response and are described as essential effectors in the immune response to tumors. This makes activated neutrophils an interesting candidate for cell therapies. Here, we review the biology of activated neutrophils in detail. We discuss the different ways neutrophils can be activated and the effect they have on other immune cells for stimulation of downstream immune response. We review the conditions where activated neutrophil therapy can be therapeutically beneficial and discuss the challenges associated with their eventual translation. Overall, this review summarizes the current state of understanding of neutrophil-based immunotherapies and their clinical potential.","PeriodicalId":9263,"journal":{"name":"Bioengineering & Translational Medicine","volume":"10 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2024-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/btm2.10704","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141980696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immunotherapy against glioblastoma using backpack-activated neutrophils 利用背包激活的中性粒细胞对胶质母细胞瘤进行免疫治疗

IF 6.1 2区医学

Bioengineering & Translational Medicine Pub Date : 2024-08-13 DOI: 10.1002/btm2.10712

Tatsuya Fukuta, Ninad Kumbhojkar, Supriya Prakash, Suyog Shaha, A. Da Silva-Candal, Kyung Soo Park, Samir Mitragotri

{"title":"Immunotherapy against glioblastoma using backpack-activated neutrophils","authors":"Tatsuya Fukuta, Ninad Kumbhojkar, Supriya Prakash, Suyog Shaha, A. Da Silva-Candal, Kyung Soo Park, Samir Mitragotri","doi":"10.1002/btm2.10712","DOIUrl":"10.1002/btm2.10712","url":null,"abstract":"Immune checkpoint inhibitors (ICIs) represent new therapeutic candidates against glioblastoma multiforme (GBM); however, their efficacy is clinically limited due to both local and systemic immunosuppressive environments. Hence, therapeutic approaches that stimulate local and systemic immune environments can improve the efficacy of ICIs. Here, we report an adoptive cell therapy employing neutrophils (NE) that are activated via surface attachment of drug-free disk-shaped backpacks, termed Cyto-Adhesive Micro-Patches (CAMPs) for treating GBM. CAMP-adhered neutrophils (NE/CAMPs) significantly improved the efficacy of an anti-PD1 antibody (aPD-1) in a subcutaneous murine GBM model (GL261). A combination of NE/CAMPs and aPD-1 completely regressed subcutaneous GL261 tumors in mice. The efficacy of NE/CAMPs against GBM was also tested in an orthotopic GL261 model. Neutrophil's ability to migrate into the brain was not affected by CAMP attachment, and intracerebral NE/CAMP accumulation was observed in mice-bearing orthotopic GBM. The combination treatment of NE/CAMPs and aPD-1 activated systemic immune responses mediated by T cells and showed improved therapeutic responses compared with aPD-1 alone in the orthotopic GBM model. These results suggest that immunomodulation with NE/CAMPs offers a potential approach for the treatment of GBM by combination with ICIs.","PeriodicalId":9263,"journal":{"name":"Bioengineering & Translational Medicine","volume":"10 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2024-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/btm2.10712","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141980705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Human adipose-derived multipotent stromal cells enriched with IL-10 modRNA improve diabetic wound healing: Trigger the macrophage phenotype shift 富含 IL-10 modRNA 的人脂肪衍生多能基质细胞可改善糖尿病伤口愈合：触发巨噬细胞表型转变

IF 6.1 2区医学

Bioengineering & Translational Medicine Pub Date : 2024-08-07 DOI: 10.1002/btm2.10711

Yuxin Zhang, Wei Wang, Liang Chen, Heng Wang, Dong Dong, Jingjing Zhu, Yu Guo, Yiqun Zhou, Tianyi Liu, Wei Fu

{"title":"Human adipose-derived multipotent stromal cells enriched with IL-10 modRNA improve diabetic wound healing: Trigger the macrophage phenotype shift","authors":"Yuxin Zhang, Wei Wang, Liang Chen, Heng Wang, Dong Dong, Jingjing Zhu, Yu Guo, Yiqun Zhou, Tianyi Liu, Wei Fu","doi":"10.1002/btm2.10711","DOIUrl":"10.1002/btm2.10711","url":null,"abstract":"Diabetic wounds present a significant challenge in regenerative medicine due to impaired healing, characterized by prolonged inflammation and deficient tissue repair, primarily caused by a skewed pro-inflammatory macrophage phenotype. This study investigates the therapeutic potential of interleukin-10 (IL-10) chemically modified mRNA (modRNA)-enriched human adipose-derived multipotent stromal cells (hADSCs) in a well-established murine model of diabetic wounds. The modRNAs used in this study were chemically modified using N1-methylpseudouridine-5′-triphosphate (m1Ψ) by substituting uridine-5-triphosphate. In vitro experiments demonstrated that IL-10 modRNA-transfected hADSCs effectively modulated macrophage polarization towards an anti-inflammatory phenotype. In vivo experiments with a well-established murine model demonstrated that transplantation of hADSCsmodIL-10 on postoperative day 5 (POD5) significantly improved wound healing outcomes, including accelerated wound closure, enhanced re-epithelialization, promoted M2 polarization, improved collagen deposition, and increased neovascularization. This study concludes that IL-10 modRNA-enriched hADSCs offer a promising therapeutic approach for diabetic wound healing, with the timing of IL-10 administration playing a crucial role in its effectiveness. These cells modulate macrophage polarization and promote tissue repair, demonstrating their potential for improving the management of diabetic wounds.","PeriodicalId":9263,"journal":{"name":"Bioengineering & Translational Medicine","volume":"10 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2024-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/btm2.10711","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141904608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bilayer 3D co-culture platform inducing the differentiation of normal fibroblasts into cancer-associated fibroblast like cells: New in vitro source to obtain cancer-associated fibroblasts 诱导正常成纤维细胞分化为类似癌症相关成纤维细胞的双层三维共培养平台：获取癌症相关成纤维细胞的新体外来源

IF 6.1 2区医学

Bioengineering & Translational Medicine Pub Date : 2024-08-05 DOI: 10.1002/btm2.10708

Yeon Ju Kim, Hyeon Song Lee, Dohyun Kim, Hwa Kyung Byun, Woong Sub Koom, Won-Gun Koh

{"title":"Bilayer 3D co-culture platform inducing the differentiation of normal fibroblasts into cancer-associated fibroblast like cells: New in vitro source to obtain cancer-associated fibroblasts","authors":"Yeon Ju Kim, Hyeon Song Lee, Dohyun Kim, Hwa Kyung Byun, Woong Sub Koom, Won-Gun Koh","doi":"10.1002/btm2.10708","DOIUrl":"10.1002/btm2.10708","url":null,"abstract":"This study presents a novel in vitro bilayer 3D co-culture platform designed to obtain cancer-associated fibroblasts (CAFs)-like cells. The platform consists of a bilayer hydrogel structure with a collagen/polyethylene glycol (PEG) hydrogel for fibroblasts as the upper layer and an alginate hydrogel for tumor cells as the lower layer. The platform enabled paracrine interactions between fibroblasts and cancer cells, which allowed for selective retrieval of activated fibroblasts through collagenase treatment for further study. Fibroblasts remained viable throughout the culture periods and showed enhanced proliferation when co-cultured with cancer cells. Morphological changes in the co-cultured fibroblasts resembling CAFs were observed, especially in the 3D microenvironment. The mRNA expression levels of CAF-related markers were significantly upregulated in 3D, but not in 2D co-culture. Proteomic analysis identified upregulated proteins associated with CAFs, further confirming the transformation of normal fibroblasts into CAF within the proposed 3D co-culture platform. Moreover, co-culture with CAF induced radio- and chemoresistance in pancreatic cancer cells (PANC-1). Survival rate of cancer cells post-irradiation and gemcitabine resistance increased significantly in the co-culture setting, highlighting the role of CAFs in promoting cancer cell survival and therapeutic resistance. These findings would contribute to understanding molecular and phenotypic changes associated with CAF activation and provide insights into potential therapeutic strategies targeting the tumor microenvironment.","PeriodicalId":9263,"journal":{"name":"Bioengineering & Translational Medicine","volume":"10 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/btm2.10708","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141895220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mesenchymal stem cells for osteoarthritis: Recent advances in related cell therapy 间充质干细胞治疗骨关节炎：相关细胞疗法的最新进展

IF 6.1 2区医学

Bioengineering & Translational Medicine Pub Date : 2024-08-05 DOI: 10.1002/btm2.10701

Jianjing Lin, Jingtao Huang, Zilu Jiao, Mengyuan Nian, Canfeng Li, Yali Dai, Shicheng Jia, Xintao Zhang

引用次数: 0

Sperm quality analyzer: A portable LED array microscope with dark-field imaging 精子质量分析仪具有暗视野成像功能的便携式 LED 阵列显微镜

IF 6.1 2区医学

Bioengineering & Translational Medicine Pub Date : 2024-08-02 DOI: 10.1002/btm2.10703

Meng Shao, Changxu Li, Xiaohao Ma, Haoyu Pan, Zeyu Ke, Rui Liu, Zhiguo Zhang, Min-Cheng Zhong, Yi Wang, Zhensheng Zhong, Fengya Lu, Xunbin Wei, Jinhua Zhou

{"title":"Sperm quality analyzer: A portable LED array microscope with dark-field imaging","authors":"Meng Shao, Changxu Li, Xiaohao Ma, Haoyu Pan, Zeyu Ke, Rui Liu, Zhiguo Zhang, Min-Cheng Zhong, Yi Wang, Zhensheng Zhong, Fengya Lu, Xunbin Wei, Jinhua Zhou","doi":"10.1002/btm2.10703","DOIUrl":"10.1002/btm2.10703","url":null,"abstract":"Sperm quality analysis plays an important role in diagnosing infertility, which is widely implemented by computer-assisted sperm analysis (CASA) of sperm-swimming imaging from commercial phase-contrast microscopy. A well-equipped microscope comes with a high cost, increasing the burden of assessment, and it also occupies a large volume. For point-of-care testing (POCT) of sperm quality, these factors are confronted with the challenges of low-cost and portable instruments. In this study, an encoded light-emitting diode (LED) array illumination is employed to achieve a portable microscope with multicontrast imaging for sperm quality analysis. This microscopy has dimensions of 16.5 × 14.0 × 25.0 cm, and its dark-field (DF) imaging provides high-contrast sperm image data which is suitable for CASA. According to DF imaging, we developed a software of LabCASA, which can used to assess the motility characteristics of sperm. Compared with TrackMate, the difference in motility parameters from our software was less than 10% in the coefficient of variation (CV). The sperm motility parameters vary with the chamber temperature, which further confirms the reliability of our system with DF imaging. The DF imaging provides strong robustness for tracking sperm's motion under different microscopes. For assessment of the motility parameters, our system can work at a lower cost with a plastic structure. This system with DF imaging is suitable for portable POCT of sperm quality analysis, which is highly cost-effective in resource-constrained circumstances.","PeriodicalId":9263,"journal":{"name":"Bioengineering & Translational Medicine","volume":"9 6","pages":""},"PeriodicalIF":6.1,"publicationDate":"2024-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/btm2.10703","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141880163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Insights into the mechanisms, regulation, and therapeutic implications of extracellular matrix stiffness in cancer 深入了解癌症细胞外基质硬度的机制、调节和治疗意义

IF 6.1 2区医学

Bioengineering & Translational Medicine Pub Date : 2024-07-31 DOI: 10.1002/btm2.10698

Ximo Zhang, Abdullah Al-Danakh, Xinqing Zhu, Dan Feng, Linlin Yang, Haotian Wu, Yingying Li, Shujing Wang, Qiwei Chen, Deyong Yang

{"title":"Insights into the mechanisms, regulation, and therapeutic implications of extracellular matrix stiffness in cancer","authors":"Ximo Zhang, Abdullah Al-Danakh, Xinqing Zhu, Dan Feng, Linlin Yang, Haotian Wu, Yingying Li, Shujing Wang, Qiwei Chen, Deyong Yang","doi":"10.1002/btm2.10698","DOIUrl":"10.1002/btm2.10698","url":null,"abstract":"The tumor microenvironment (TME) is critical for cancer initiation, growth, metastasis, and therapeutic resistance. The extracellular matrix (ECM) is a significant tumor component that serves various functions, including mechanical support, TME regulation, and signal molecule generation. The quantity and cross-linking status of ECM components are crucial factors in tumor development, as they determine tissue stiffness and the interaction between stiff TME and cancer cells, resulting in aberrant mechanotransduction, proliferation, migration, invasion, angiogenesis, immune evasion, and treatment resistance. Therefore, broad knowledge of ECM dysregulation in the TME might aid in developing innovative cancer therapies. This review summarized the available information on major ECM components, their functions, factors that increase and decrease matrix stiffness, and related signaling pathways that interplay between cancer cells and the ECM in TME. Moreover, mechanotransduction alters during tumorogenesis, and current drug therapy based on ECM as targets, as well as future efforts in ECM and cancer, are also discussed.","PeriodicalId":9263,"journal":{"name":"Bioengineering & Translational Medicine","volume":"10 1","pages":""},"PeriodicalIF":6.1,"publicationDate":"2024-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/btm2.10698","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141877357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0