Bioengineering & Translational Medicine最新文献

Engineering macrophage phenotype switching via nucleotide‐binding oligomerization domain‐like receptor protein 3 inflammasome inhibition: A translational approach using antibiotic cement for diabetic foot ulcers 通过核苷酸结合寡聚化结构域样受体蛋白3炎性体抑制工程巨噬细胞表型转换：使用抗生素水泥治疗糖尿病足溃疡的翻译方法

IF 7.4 2区医学

Bioengineering & Translational Medicine Pub Date : 2025-10-15 DOI: 10.1002/btm2.70073

Yi Zhang, Fusen Jia, Ming Li, Xin Tang, Fei Yang

{"title":"Engineering macrophage phenotype switching via nucleotide‐binding oligomerization domain‐like receptor protein 3 inflammasome inhibition: A translational approach using antibiotic cement for diabetic foot ulcers","authors":"Yi Zhang, Fusen Jia, Ming Li, Xin Tang, Fei Yang","doi":"10.1002/btm2.70073","DOIUrl":"https://doi.org/10.1002/btm2.70073","url":null,"abstract":"Diabetic foot ulcers (DFUs), a debilitating complication of diabetes, are exacerbated by persistent inflammation that disrupts wound repair. This study explores the therapeutic potential of antibiotic‐loaded bone cement (ALBC) in modulating NLRP3 inflammasome activation and macrophage polarization to resolve chronic inflammation and accelerate healing. Using db/db diabetic mice with dorsal wounds and RAW264.7 macrophages under high‐glucose conditions, we tested graded ALBC doses (high‐dose ALBC, low‐dose ALBC, and medium‐dose ALBC) both in vivo and in vitro. Multi‐modal analyses—including cytokine profiling (enzyme‐linked immunosorbent assay), macrophage phenotyping (flow cytometry/immunofluorescence), and molecular pathway interrogation (reverse transcription quantitative PCR/Western blot)—revealed that ALBC dose‐dependently suppressed NLRP3 inflammasome assembly, reduced IL‐1β/IL‐18 secretion, and skewed macrophages toward anti‐inflammatory M2 phenotypes. Pharmacological NLRP3 activation reversed these effects, confirming pathway specificity. ALBC‐treated wounds exhibited accelerated re‐epithelialization, collagen deposition, and angiogenesis, correlating with attenuated systemic inflammation. Crucially, clinical DFU samples mirrored preclinical findings, showing NLRP3 downregulation and M2 dominance in ALBC‐responsive cases. These results demonstrate that ALBC orchestrates immunometabolic reprogramming by silencing NLRP3‐driven inflammation and fostering pro‐reparative macrophage responses. By bridging biomaterial engineering with immunomodulation, this work advances a translatable strategy for refractory DFU management, offering a dual‐action therapeutic platform that combines localized antibiotic delivery with microenvironmental immune reset.","PeriodicalId":9263,"journal":{"name":"Bioengineering & Translational Medicine","volume":"84 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145295435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Carbon nanomaterials: Exploring new frontiers in wound healing therapy 碳纳米材料：探索伤口愈合治疗的新领域

IF 7.4 2区医学

Bioengineering & Translational Medicine Pub Date : 2025-10-14 DOI: 10.1002/btm2.70071

Pegah Madaninasab, Mahsa Mohammadzadeh, Sheyda Labbaf

引用次数: 0

Development and validation of a computational tool to predict treatment outcomes in cells from high‐grade serous ovarian cancer patients 一种预测高级别浆液性卵巢癌患者细胞治疗结果的计算工具的开发和验证

IF 7.4 2区医学

Bioengineering & Translational Medicine Pub Date : 2025-10-07 DOI: 10.1002/btm2.70082

Marilisa Cortesi, Dongli Liu, Elyse Powell, Ellen Barlow, Kristina Warton, Emanuele Giordano, Caroline E. Ford

引用次数: 0

Renal‐targeted exosomes inhibiting miR‐182‐5p for treatment of renal ischemia–reperfusion injury 肾靶向外泌体抑制miR - 182 - 5p治疗肾缺血再灌注损伤

IF 7.4 2区医学

Bioengineering & Translational Medicine Pub Date : 2025-10-03 DOI: 10.1002/btm2.70081

Zepeng Li, Shirui Sun, Zhenting Zhao, Yingcong Guo, Qi He, Mei Yang, Jin Zheng, Jianhui Li, Wujun Xue, Chenguang Ding

{"title":"Renal‐targeted exosomes inhibiting miR‐182‐5p for treatment of renal ischemia–reperfusion injury","authors":"Zepeng Li, Shirui Sun, Zhenting Zhao, Yingcong Guo, Qi He, Mei Yang, Jin Zheng, Jianhui Li, Wujun Xue, Chenguang Ding","doi":"10.1002/btm2.70081","DOIUrl":"https://doi.org/10.1002/btm2.70081","url":null,"abstract":"Renal ischemia–reperfusion injury (IRI) is a significant condition that leads to acute kidney injury, exacerbating the progression of renal failure clinically and affecting the patient's prognosis. Following the identification of miR‐182‐5p as a significant molecule in IRI, we conducted a detailed analysis of its potential downstream genes and assessed its involvement in the SIRT1/Nrf2/ferroptosis pathway. To validate these findings in vivo, we implemented an exosome‐mediated drug delivery protocol and assessed its therapeutic efficacy in C57BL/6. miR‐182‐5p exhibited a notable upregulation in renal IRI. Utilizing bioinformatics approaches, the study further investigated and validated its downstream SIRT1/Nrf2 pathway, establishing its role in ferroptosis. By employing LTHVVWL(LTH)‐anchored exosomes, the delivery of miR‐182‐5p to the kidney was significantly improved, thereby illustrating its potential efficacy in mitigating renal IRI. The findings of our study demonstrated that miR‐182‐5p suppressed SIRT1/Nrf2 activity and facilitated ferroptosis, suggesting its potential as a therapeutic target for clinical IRI treatment. The inhibition of miR‐182‐5p via LTH‐anchored exosomes was shown to significantly mitigate renal IRI, providing a novel approach for the development of miRNA‐based therapeutic drug delivery systems.","PeriodicalId":9263,"journal":{"name":"Bioengineering & Translational Medicine","volume":"157 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145209757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mechanical interplay between adipose tissues and disease progression 脂肪组织与疾病进展之间的机械相互作用

IF 7.4 2区医学

Bioengineering & Translational Medicine Pub Date : 2025-10-03 DOI: 10.1002/btm2.70065

Hangyu Zhou, Danni Zhou, Miaoben Wu, Yuye Huang, Enxing Yu, Jianing Xie, Yangjian Wang, Shuqin Chen, Qinghua Song, Kailei Xu, Peng Wei

{"title":"Mechanical interplay between adipose tissues and disease progression","authors":"Hangyu Zhou, Danni Zhou, Miaoben Wu, Yuye Huang, Enxing Yu, Jianing Xie, Yangjian Wang, Shuqin Chen, Qinghua Song, Kailei Xu, Peng Wei","doi":"10.1002/btm2.70065","DOIUrl":"https://doi.org/10.1002/btm2.70065","url":null,"abstract":"Over the past two decades, an increasing body of evidence has underscored the significant role of the mechanical properties of biological tissues in maintaining tissue functions and regulating cellular changes, such as proliferation, migration, and differentiation. Throughout disease progression, such as in cancers, bone defects, and cardiac conditions, the mechanical microenvironment of tissues can undergo dramatic changes, exerting profound effects on disease development. Adipose tissues are inherently mechanosensitive and mechanoresponsive, continually exposed to various mechanical stresses in daily life. The hypertrophy and accumulation of adipocytes can lead to obesity, a condition strongly associated with numerous health risks, like diabetes and cancers. In this review, we aim to elucidate the reciprocal mechanical interaction between adipose tissues and disease progression, encompassing cancers, bone defects, and cardiac pathologies. The existing literature suggests that alterations in the mechanical microenvironment during disease advancement may impede adipogenic differentiation, induce adipocyte dedifferentiation, and escalate the secretion of inflammatory cytokines. Conversely, dysregulation of adipose tissues can result in the deposition of extracellular matrix components, stiffening the microenvironment and fostering disease progression in a cyclical fashion. Therefore, in future treatments of related diseases, a combined approach integrating mechanotherapeutics and obesity management holds promise for achieving the desired enhanced therapeutic outcomes.","PeriodicalId":9263,"journal":{"name":"Bioengineering & Translational Medicine","volume":"74 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145209774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Overcoming challenges in cartilage regeneration: The role of chondrogenic inducers 克服软骨再生的挑战：软骨诱导因子的作用

IF 7.4 2区医学

Bioengineering & Translational Medicine Pub Date : 2025-09-29 DOI: 10.1002/btm2.70079

Manh Tuong Nguyen, Stan Gronthos, Yunpeng Zhao, Vashe Chandrakanthan, Vi Khanh Truong, Krasimir Vasilev

{"title":"Overcoming challenges in cartilage regeneration: The role of chondrogenic inducers","authors":"Manh Tuong Nguyen, Stan Gronthos, Yunpeng Zhao, Vashe Chandrakanthan, Vi Khanh Truong, Krasimir Vasilev","doi":"10.1002/btm2.70079","DOIUrl":"https://doi.org/10.1002/btm2.70079","url":null,"abstract":"Cartilage regeneration presents unique challenges due to its avascular structure, sparse cell population, and limited regenerative capacity. Recent years have seen significant advancements in the field, which warrant an integrated review that connects chondrogenesis and its practical application. This review aims to deliver comprehensive and analytical guidelines for understanding the complex process of chondrogenesis, emphasizing its critical role in cartilage regeneration. It reviews key inducers such as growth factors, mechanical stimuli, hypoxia, and electric fields, as well as their synergistic integration with biomaterials to facilitate effective strategies for repairing and regenerating damaged cartilage tissue. In addition to exploring these advancements, the paper also provides a critical evaluation of current methods used to assess chondrogenesis in in vitro and in vivo models, identifying gaps and possibilities for improvement. A particular focus is placed on addressing the translational challenges that hinder the clinical implementation of cutting‐edge research findings, offering actionable strategies to bridge the gap between laboratory discoveries and patient outcomes. By examining emerging trends and consolidating recent innovations, this review aims to offer a holistic perspective on cartilage repair. It serves as a guide for researchers and clinicians, advocating for collaborative, interdisciplinary approaches to advance the field and deliver improved therapeutic solutions for cartilage‐related conditions.","PeriodicalId":9263,"journal":{"name":"Bioengineering & Translational Medicine","volume":"20 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145182760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioprinting revolution: Innovative design of 3D bioactive scaffolds for living organs and transdermal tissues 生物打印革命：活体器官和透皮组织的3D生物活性支架的创新设计

IF 7.4 2区医学

Bioengineering & Translational Medicine Pub Date : 2025-09-29 DOI: 10.1002/btm2.70080

Seydanur Yücer, Begüm Sarac, Fatih Ciftci

引用次数: 0

Biomaterials‐driven stem cell therapy for tissue repair and functional rehabilitation after ischemic stroke 生物材料驱动的干细胞治疗用于缺血性中风后的组织修复和功能康复

IF 7.4 2区医学

Bioengineering & Translational Medicine Pub Date : 2025-09-27 DOI: 10.1002/btm2.70060

Mengjie Wang, Yuanyuan Ran, Jianshen Liang, Fanglei Li, Ning Li, Zitong Ding, Jianing Xi, Wei Su, Lin Ye, Zongjian Liu

{"title":"Biomaterials‐driven stem cell therapy for tissue repair and functional rehabilitation after ischemic stroke","authors":"Mengjie Wang, Yuanyuan Ran, Jianshen Liang, Fanglei Li, Ning Li, Zitong Ding, Jianing Xi, Wei Su, Lin Ye, Zongjian Liu","doi":"10.1002/btm2.70060","DOIUrl":"https://doi.org/10.1002/btm2.70060","url":null,"abstract":"Ischemic stroke is a serious cerebrovascular disease with limited effective treatments. While stem cell therapy shows promise, ensuring cell survival and integration into neural networks remains a challenge. Recent research shows tissue engineering can greatly fix these flaws. Notably, we focus on the structure–activity relationship of biomaterials. How cell behavior can be most beneficially regulated by changes in the physical structure of the cell carrier itself is certainly a new perspective for cost saving and effectiveness increasing compared to the delivery of expensive biotrophic factors. However, there is a lack of research on biomaterials applied to ischemic stroke, especially in combination with stem cells. No biomaterial has even been approved for clinical trials in stroke. We provide a systematic summary of biomaterials‐driven stem cell therapy for ischemic stroke in terms of pathomechanisms, applications, and clinical translational challenges; we attempt to build a bridge from laboratory research to clinical translation in stroke treatment.","PeriodicalId":9263,"journal":{"name":"Bioengineering & Translational Medicine","volume":"16 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145153619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Glioma chemotherapeutic resistance is tied to membrane electrophysiological properties and glycosylation 胶质瘤化疗耐药与膜电生理特性和糖基化有关

IF 7.4 2区医学

Bioengineering & Translational Medicine Pub Date : 2025-09-23 DOI: 10.1002/btm2.70069

Alan Y. L. Jiang, Andrew R. Yale, J. Nicole Hanamoto, Nicole S. Lav, Vi Phuong Dang, Clarissa C. Ro, Christopher R. Douglas, Kaijun Di, Jacob Deyell, Daniela A. Bota, Lisa A. Flanagan

{"title":"Glioma chemotherapeutic resistance is tied to membrane electrophysiological properties and glycosylation","authors":"Alan Y. L. Jiang, Andrew R. Yale, J. Nicole Hanamoto, Nicole S. Lav, Vi Phuong Dang, Clarissa C. Ro, Christopher R. Douglas, Kaijun Di, Jacob Deyell, Daniela A. Bota, Lisa A. Flanagan","doi":"10.1002/btm2.70069","DOIUrl":"https://doi.org/10.1002/btm2.70069","url":null,"abstract":"Diffuse gliomas are brain tumors that include oligodendroglioma, astrocytoma, and glioblastoma (GBM), the most common and deadly primary brain tumor. A major challenge in glioma treatment is resistance to the first‐line chemotherapeutic, temozolomide (TMZ). Plasma membrane properties of cells with increased chemotherapeutic resistance are not well understood, despite the fact that the membrane is the first point of contact with the environment and greatly shapes cell behavior. Plasma membrane glycosylation impacts cell function, and we found significant differences in glycosylation of TMZ‐resistant cells. We further identified plasma membrane electrophysiological properties predicting glioma cell TMZ resistance. We enriched cells with higher TMZ resistance by sorting glioma cells based on electrophysiological properties, indicating the relevance of membrane properties to chemotherapeutic resistance. These findings could lead to rapid separation methods for patient tumor cells, a better understanding of the molecular profiles of resistant cells, and novel treatment options for gliomas.","PeriodicalId":9263,"journal":{"name":"Bioengineering & Translational Medicine","volume":"18 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145116526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel brachytherapy and chemotherapy integrated ureteral stent: In vitro and in vivo study 一种新型近距离化疗输尿管支架：体外和体内研究

IF 7.4 2区医学

Bioengineering & Translational Medicine Pub Date : 2025-09-19 DOI: 10.1002/btm2.70077

Xiaotian Yang, Xueliang Zhou, Zhanyun Zhou, Yipu Li, Chengzhi Zhang, Yingqi Liu, Xiaohan Ma, Yanan Li, Yebin Wang, Dechao Jiao

{"title":"A novel brachytherapy and chemotherapy integrated ureteral stent: In vitro and in vivo study","authors":"Xiaotian Yang, Xueliang Zhou, Zhanyun Zhou, Yipu Li, Chengzhi Zhang, Yingqi Liu, Xiaohan Ma, Yanan Li, Yebin Wang, Dechao Jiao","doi":"10.1002/btm2.70077","DOIUrl":"https://doi.org/10.1002/btm2.70077","url":null,"abstract":"Ureteral carcinoma remains a major clinical challenge and requires effective localized treatment. Here, we report a novel 125I seed brachytherapy (ISB) and doxorubicin (DOX) chemotherapy integrated ureteral stent (IUS), which enables simultaneous urinary drainage and chemoradiotherapy. This study was divided into three parts. First, ISB and DOX significantly reduced T24 cell viability and inhibited migration and invasion in an in vivo study (p < 0.01). Second, a T24 xenograft mouse model demonstrated that the (DOX + ISB) group exhibited greater tumor suppression than the DOX (p = 0.08) and ISB (p = 0.02) groups, with decreased Ki‐67 and Bcl‐2 expression and increased apoptosis (all p < 0.01) in an in vitro study. Third, the IUS was successfully implanted in normal beagle dogs (n = 30) without surgical complications. The ureteral diameter increased with increasing cumulative brachytherapy and sustained DOX release (p < 0.05). Histological analysis revealed progressive tissue damage and fibrosis, with increased expression of α‐SMA, Caspase‐3, and Collagen‐1 in the 0.8 mCi + 20 mg DOX group (p < 0.05), whereas PCNA expression was highest in the Control group (0 mCi + 0 mg DOX). In conclusion, the newly designed IUS is safe and technically feasible in animals; clinical studies will be required to evaluate its use in humans.","PeriodicalId":9263,"journal":{"name":"Bioengineering & Translational Medicine","volume":"129 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145088975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0