Bioengineering & Translational Medicine最新文献_第2页

Engineered probiotic ameliorates hyperlipidemia and atherosclerosis by secreting PCSK9 nanobodies and regulating gut microbiota 工程益生菌通过分泌PCSK9纳米体和调节肠道微生物群来改善高脂血症和动脉粥样硬化

IF 7.4 2区医学

Bioengineering & Translational Medicine Pub Date : 2025-09-19 DOI: 10.1002/btm2.70076

Chuan Wang, Junyue Xing, Huan Zhao, Xiru Chen, Zongfeng Niu, Xiaohan Ma, Yuesheng Gui, Xinkun Qi, Yingchao Shi, Xiaolei Cheng, Dongdong Jian, Chao Shi, Hao Tang, Zhen Li

{"title":"Engineered probiotic ameliorates hyperlipidemia and atherosclerosis by secreting PCSK9 nanobodies and regulating gut microbiota","authors":"Chuan Wang, Junyue Xing, Huan Zhao, Xiru Chen, Zongfeng Niu, Xiaohan Ma, Yuesheng Gui, Xinkun Qi, Yingchao Shi, Xiaolei Cheng, Dongdong Jian, Chao Shi, Hao Tang, Zhen Li","doi":"10.1002/btm2.70076","DOIUrl":"https://doi.org/10.1002/btm2.70076","url":null,"abstract":"Elevated levels of low‐density lipoprotein cholesterol (LDL‐C) play a critical role in the onset and progression of cardiovascular disease (CVD). Inhibitors or monoclonal antibody drugs targeting pro‐protein convertase subtilisin/kexin type 9 (PCSK9) are novel cholesterol‐lowering medications that can effectively reduce serum LDL‐C levels. However, these drugs are usually expensive and require injections, which can reduce patient compliance and increase the financial burden. In this study, we constructed an engineered probiotic strain containing a prokaryotic expression element and a high‐affinity fragment of the human PCSK9 nanobody (PCSK9nb). The engineered bacterium was evaluated in vitro and in vivo for its ability to express and release PCSK9nb, as well as for its biocompatibility and stability. The therapeutic potential of the engineered probiotics was confirmed using mouse models of hyperlipidemia and atherosclerosis. We analyzed differences in mouse gut microbiota using high‐throughput sequencing and compared the therapeutic efficacy of the engineered bacteria with that of atorvastatin in a mouse model of hyperlipidemia. The engineered bacteria were found to express and release PCSK9nb in vivo after oral administration, achieving the effect of lowering serum cholesterol levels, alleviating atherosclerosis, and reducing body weight. In vivo, PCSK9nb was found to increase hepatic LDL receptor (LDLR) expression levels, decrease serum LDL‐C content, regulate the diversity and community structure of gut microbiota, reduce lipid accumulation in the liver, and decrease systemic inflammation. By comparing their efficacy with that of statins, the engineered probiotics demonstrated similar therapeutic effects. The research results provide a new strategy for the development of orally delivered PCSK9 antibody drugs, reducing healthcare costs and minimizing statin drug tolerance.","PeriodicalId":9263,"journal":{"name":"Bioengineering & Translational Medicine","volume":"154 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145088976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Near Infrared‐light responsive chlorin e6 pro‐drug micellar photodynamic therapy for oral cancer 近红外光响应氯e6药物前胶束光动力治疗口腔癌

IF 7.4 2区医学

Bioengineering & Translational Medicine Pub Date : 2025-09-16 DOI: 10.1002/btm2.70036

Milan Paul, Swati Biswas

{"title":"Near Infrared‐light responsive chlorin e6 pro‐drug micellar photodynamic therapy for oral cancer","authors":"Milan Paul, Swati Biswas","doi":"10.1002/btm2.70036","DOIUrl":"https://doi.org/10.1002/btm2.70036","url":null,"abstract":"A major concern of conventional photodynamic therapy is its non‐specific toxicity due to off‐site drug accumulation. Micelles tend to localize the drug to the tumor site. However, rapid drug release at high concentrations from the micelles to kill the cancer cells remains a formidable task. In this manuscript, we have introduced the 2‐nitrobenzyl (2NB)‐moiety as the linker between mPEG and the photosensitizer, chlorin e6 (Ce6), to prepare the conjugate, mPEG(2‐nitrobenzyl)Ce6. We envision that 2NB as a linker between hydrophobic, Ce6, and hydrophilic mPEG would be more effective in releasing Ce6 by disassembling PEGylated 2‐nitrobenzyl chlorin e6 (mPNCe6) Ms. Characterization through Fourier transform infrared spectroscopy and 1H, 13C nuclear magnetic resonance spectra validated the successful synthesis of the conjugate. By conjugating Ce6 into the hydrophobic core of the micelles, exposure to near‐infrared light significantly hastened the dissociation of the micelles, facilitating a controlled and rapid release of Ce6's hydrophobic components within the micelles. A cellular uptake study was performed, showing that Ce6 conjugation has improved the uptake of Ce6. The cell viability assay revealed that the formulation had shown concentration‐dependent cytotoxicity upon laser irradiation. mPNCe6 group with laser irradiation has generated abundant reactive oxygen species (ROS) inside cells and exhibited green solid fluorescence, indicating the efficient delivery of Ce6 by mPNCe6 micelles and its excellent ROS generation ability inside cells upon laser irradiation. Further, in vivo studies on MOC2 tumor‐bearing mice demonstrate reduced tumor growth, lung metastasis, and drug accumulation in the tumor region. The developed nanomedicine could be a potential treatment strategy for oral cancer, minimizing the occurrence of lung metastasis.","PeriodicalId":9263,"journal":{"name":"Bioengineering & Translational Medicine","volume":"18 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145072137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Generation and ex vivo characterization of a full‐thickness substitute of the human urethra by tissue engineering 组织工程制备全层人类尿道替代物及体外特性研究

IF 7.4 2区医学

Bioengineering & Translational Medicine Pub Date : 2025-09-16 DOI: 10.1002/btm2.70049

David Sánchez‐Porras, Miguel Etayo‐Escanilla, José‐Andrés Moreno‐Delgado, María del Mar Lozano‐Martí, Fabiola Bermejo‐Casares, Miguel Alaminos, Jesús Chato‐Astrain, Fernando Campos, M. Carmen Sánchez‐Quevedo, Ricardo Fernández‐Valadés

{"title":"Generation and ex vivo characterization of a full‐thickness substitute of the human urethra by tissue engineering","authors":"David Sánchez‐Porras, Miguel Etayo‐Escanilla, José‐Andrés Moreno‐Delgado, María del Mar Lozano‐Martí, Fabiola Bermejo‐Casares, Miguel Alaminos, Jesús Chato‐Astrain, Fernando Campos, M. Carmen Sánchez‐Quevedo, Ricardo Fernández‐Valadés","doi":"10.1002/btm2.70049","DOIUrl":"https://doi.org/10.1002/btm2.70049","url":null,"abstract":"Tissue engineering may offer efficient alternatives for the surgical repair of severe conditions affecting the human urethra. However, development of tubular full‐thickness substitutes is challenging. In this work, we have generated and evaluated ex vivo a novel full‐thickness human urethra substitute (FHUS) containing its three main layers: the urethral mucosa (UM), the spongy layer (SP), and the tunica albuginea (AL). Results first showed that the generation of a FHUS significantly improved the biomechanical properties of this artificial tissue as compared to the individual layers, although the resistance of the native urethra was not reached. At the structural level, we found that FHUS shared important histological similarities with the native urethra. Analysis of the individual layers showed that UM had a stratified epithelium that expressed several epithelial markers, including cytokeratins CK7 and CK14, uroplakin 1b, and the intercellular junction proteins desmoplakin, tight junction protein 1, and claudin. At the stromal level, UM tended to increase the presence of collagen fibers and versican with time. The SP layer displayed abundant CD31 and CD34‐positive blood vessels, but small amounts of collagen and proteoglycans. The AL layer showed scattered smooth muscle cells expressing α‐smooth muscle actin, smoothelin, and desmin cell markers, and contained low amounts of collagen and proteoglycans. Analysis of the basement membrane components collagen IV and laminin revealed their progressive development with time, especially collagen IV. These results confirm the possibility of developing a partially biomimetic full‐thickness substitute of human urethra that might have potential clinical usefulness for the clinical repair of severe urethral lesions.","PeriodicalId":9263,"journal":{"name":"Bioengineering & Translational Medicine","volume":"17 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145072138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Design and pharmacodynamic study of live biotherapeutic products with efficient degradation of branched‐chain amino acids 高效降解支链氨基酸的活性生物治疗产品的设计和药效学研究

IF 7.4 2区医学

Bioengineering & Translational Medicine Pub Date : 2025-09-15 DOI: 10.1002/btm2.70075

Zhaowei Chen, Jingyi Xu, Huayue Zhang, Yuezhu Wang, Mingjie Li, Yixiao Wu, Yongqiang Zhu, Yue Liu, Haiyang Xia, Huajun Zheng

{"title":"Design and pharmacodynamic study of live biotherapeutic products with efficient degradation of branched‐chain amino acids","authors":"Zhaowei Chen, Jingyi Xu, Huayue Zhang, Yuezhu Wang, Mingjie Li, Yixiao Wu, Yongqiang Zhu, Yue Liu, Haiyang Xia, Huajun Zheng","doi":"10.1002/btm2.70075","DOIUrl":"https://doi.org/10.1002/btm2.70075","url":null,"abstract":"The homeostasis of branched‐chain amino acids (BCAAs) plays a crucial role in maintaining health, and the accumulation of BCAAs can lead to various diseases. Therefore, exogenous degradation or conversion of excessive BCAAs may help alleviate diseases caused by BCAA accumulation, such as maple syrup urine disease. This study utilized synthetic biology approaches to engineer two strains for efficient BCAA catabolism successfully—ECN‐Deg and ECN‐Tra—by integrating specific metabolic pathways into the chassis strain, Escherichia coli Nissle 1917 (ECN). ECN‐Deg integrates a metabolic module for BCAA degradation, while ECN‐Tra integrates a metabolic module for BCAA transformation. Both engineered strains demonstrate efficient BCAA catabolism in vitro and in vivo. In a high‐BCAA mouse model, ECN‐Deg and ECN‐Tra alleviated liver and ileal damage caused by excessive BCAAs and reduced systemic inflammation levels. Furthermore, ECN‐Deg and ECN‐Tra were able to modulate the gut microbiota, increasing the richness of Akkermansia muciniphila and Mucispirillum schaedleri, which are associated with health benefits. Additionally, they reduced the richness of the pathogenic bacterium Streptococcus pasteurianus. Thus, this study lays the foundation for the development of probiotics for the treatment of BCAAs metabolic disorders and BCAAs‐related chronic diseases.","PeriodicalId":9263,"journal":{"name":"Bioengineering & Translational Medicine","volume":"171 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145072140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Engineered endothelial cells targeting and dihydrotanshinone I loaded bacterial extracellular vesicles for atherosclerosis therapy 工程内皮细胞靶向和负载二氢丹参酮的细菌细胞外囊泡用于动脉粥样硬化治疗

IF 7.4 2区医学

Bioengineering & Translational Medicine Pub Date : 2025-09-15 DOI: 10.1002/btm2.70074

Rong‐Rong Zhu, Xue‐Liang Zhou, Yan‐Wei Liu, Ri Xu, Peng Deng, Zhong‐Yong Liu

{"title":"Engineered endothelial cells targeting and dihydrotanshinone I loaded bacterial extracellular vesicles for atherosclerosis therapy","authors":"Rong‐Rong Zhu, Xue‐Liang Zhou, Yan‐Wei Liu, Ri Xu, Peng Deng, Zhong‐Yong Liu","doi":"10.1002/btm2.70074","DOIUrl":"https://doi.org/10.1002/btm2.70074","url":null,"abstract":"Atherosclerosis (AS) is a complex cardiovascular disease characterized by endothelial dysfunction, dyslipidemia, and immune‐inflammatory responses, leading to arterial plaque formation and potentially fatal complications such as myocardial infarction and stroke. Traditional treatments, such as statins, often pose challenges due to their side effects and limited efficacy. In this study, we explore a novel therapeutic approach utilizing engineered endothelial cells (ECs) targeting probiotic extracellular vesicles loaded with dihydrotanshinone I (DHT) (EC‐BEVsDHT), a bioactive compound derived from Danshen (Salvia miltiorrhiza Bunge). With the characterization of EC‐BEVsDHT by transmission electron microscope and nanoparticle tracking analysis, EC‐BEVsDHT exhibited typical spherical morphology and particle size distribution. High‐performance liquid chromatography coupled with tandem mass spectrometric confirmed the expression of the ECs‐targeting peptide VSSSTPR in EC‐BEVsDHT and EC‐BEVsDHT. We further investigated the anti‐atherosclerotic effects and molecular mechanisms of EC‐BEVsDHT on human umbilical vein endothelial cells (HUVECs) and Apolipoprotein E‐deficient (ApoE−/−) C57BL/6J mice. We found that EC‐BEVsDHT attenuated oxidized low‐density lipoprotein induced HUVECs injury in vitro and decreased AS in ApoE−/− mice in vivo. Our findings suggest that EC‐BEVsDHT hold promise as a safe and effective therapeutic strategy for AS, offering potential advantages over traditional treatments.","PeriodicalId":9263,"journal":{"name":"Bioengineering & Translational Medicine","volume":"17 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145072139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancing peripheral nerve regeneration through NaOH‐based decellularization of human nerve tissue 通过基于NaOH的人神经组织脱细胞增强周围神经再生

IF 7.4 2区医学

Bioengineering & Translational Medicine Pub Date : 2025-09-13 DOI: 10.1002/btm2.70072

Subin Kim, Seong Hyuk Park, Jiyeon Mun, Soon Won Jung, Won Jai Lee, Dong Won Lee, Kee‐Won Lee

{"title":"Enhancing peripheral nerve regeneration through NaOH‐based decellularization of human nerve tissue","authors":"Subin Kim, Seong Hyuk Park, Jiyeon Mun, Soon Won Jung, Won Jai Lee, Dong Won Lee, Kee‐Won Lee","doi":"10.1002/btm2.70072","DOIUrl":"https://doi.org/10.1002/btm2.70072","url":null,"abstract":"Peripheral nerves are vulnerable to trauma, pressure, and surgical injuries, complicating the regeneration process. While the autograft remains the gold standard for recovery, limitations such as tissue availability and donor site morbidities have led to the exploration of the allografts. However, conventional detergent‐based decellularization methods in preparing allografts often cause residual toxicity and damage to the extracellular matrix (ECM). To address such challenges, we propose a sodium hydroxide (NaOH)‐based decellularization technique that minimizes harmful residues. Our findings demonstrate that this method effectively removes inflammatory materials while preserving the ECM components and structures, and significantly reduces lipid and detergent residues. In vitro studies confirmed that the human nerves processed with the NaOH‐based decellularization technique show low cytotoxicity and support elevated cell viability and proliferation. We further compared the performance of NaOH‐based decellularized human nerves with that of autografts through an in vivo rabbit sciatic nerve defect model. NaOH‐based decellularized nerves showed functional recovery comparable to autografts. Our findings demonstrate structural regeneration through neurofilament and laminin expression, indicating recovery levels similar to those of autografts. This study highlights that decellularized human nerve grafts through the NaOH‐based protocol can promote nerve regeneration comparable to autografts, which can offer a safe and effective option for the treatment and reconstruction of peripheral nerve defects.","PeriodicalId":9263,"journal":{"name":"Bioengineering & Translational Medicine","volume":"53 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145072141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cell‐embedded microgels as emerging miniature 3D tissue‐mimics toward biochip‐based toxicity screening 细胞嵌入微凝胶作为新兴的微型3D组织模拟物用于基于生物芯片的毒性筛选

IF 7.4 2区医学

Bioengineering & Translational Medicine Pub Date : 2025-09-09 DOI: 10.1002/btm2.70061

Margaux Delafosse, Estelle Regnault, Jasmin Gebauer‐Barrett, Andreas Manz, Baeckkyoung Sung

{"title":"Cell‐embedded microgels as emerging miniature 3D tissue‐mimics toward biochip‐based toxicity screening","authors":"Margaux Delafosse, Estelle Regnault, Jasmin Gebauer‐Barrett, Andreas Manz, Baeckkyoung Sung","doi":"10.1002/btm2.70061","DOIUrl":"https://doi.org/10.1002/btm2.70061","url":null,"abstract":"Recent developments in synthetic three‐dimensional (3D) gel microenvironments for cell culture have enabled the advancement of bioengineered organ‐specific cell niches that resemble the native 3D tissue architecture and mechanics. In particular, the application of 3D cell cultures based on miniaturized hydrogel scaffolds for toxicological analyses is attracting increasing interest because of their facile adaptability to on‐chip systems and potential as novel in vitro screening tools. We summarize the current progress in microgel‐based 3D cells integrated into biochip platforms and their utilization for the in vitro toxicity evaluation of chemicals and drug candidates. We emphasize the development of tissue‐mimicking microgel systems combined with automated gel microarray chips and organ‐on‐a‐chip devices. This review begins with the microscale hydrogel scaffolds that encapsulate mammalian cells and are used for in vitro tissue mimicry purposes. Furthermore, an overview of microgel‐based tissue modeling approaches to toxicity testing and screening is provided, along with their technical advantages in drug discovery and alternatives to animal testing.","PeriodicalId":9263,"journal":{"name":"Bioengineering & Translational Medicine","volume":"46 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145035155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Low‐cost, handheld, multi‐pulse electroporators for simplified nucleic acid delivery in skin 低成本，手持式，多脉冲电穿孔器，用于简化核酸在皮肤中的传递

IF 7.4 2区医学

Bioengineering & Translational Medicine Pub Date : 2025-09-09 DOI: 10.1002/btm2.70070

Pankaj Rohilla, Erkan Azizoglu, Sion Park, Atharva Lele, Mark R. Prausnitz, Saad Bhamla

{"title":"Low‐cost, handheld, multi‐pulse electroporators for simplified nucleic acid delivery in skin","authors":"Pankaj Rohilla, Erkan Azizoglu, Sion Park, Atharva Lele, Mark R. Prausnitz, Saad Bhamla","doi":"10.1002/btm2.70070","DOIUrl":"https://doi.org/10.1002/btm2.70070","url":null,"abstract":"Electroporation‐mediated delivery offers a promising alternative to carrier‐based nucleic acid delivery methods for vaccination and therapeutic applications. Carrier‐based systems like lipid nanoparticles and viral vectors often suffer from poor in vivo stability, immunogenicity, toxicity, and off‐target effects. To overcome the high cost, bulkiness, lack of portability, and painful administration of traditional electroporators, we developed the RotoPatch family of small, low‐cost, hand‐held piezoelectric electroporators that use microneedle electrodes for intradermal delivery of nucleic acids. Notably, these RotoPatch devices use a single rotary motion to administer multiple electroporation pulses through microneedle electrodes, that localize the electric field to the upper layers of the skin. In animals, RotoPatch facilitated greater intracellular uptake of firefly luciferase‐encoded mRNA in mice and green fluorescent protein‐encoded plasmid DNA in rats, as confirmed by bioluminescence and fluorescence imaging, respectively. RotoPatch produced similar in vivo expression as electroporation using a manually actuated, multi‐pulse piezoelectric electroporator (ePatch) and a battery‐powered, multi‐pulse electroporator (eIgniter). These findings highlight the potential of multi‐pulse piezoelectric microneedle electroporation for intradermal nucleic acid delivery as a platform for gene therapy and vaccination.","PeriodicalId":9263,"journal":{"name":"Bioengineering & Translational Medicine","volume":"66 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145035156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A digital ELISA for multiplexed detection of allergen‐specific IgE against Der p 1, Der p 2, and Der p 23 一种用于多重检测Der p1， Der p2和Der p23的过敏原特异性IgE的数字ELISA

IF 7.4 2区医学

Bioengineering & Translational Medicine Pub Date : 2025-08-29 DOI: 10.1002/btm2.70068

Feifei Han, Shih‐Mo Yang, Ju Xue, Wanying Xie, Yuanfen Liao, Qi Cheng, Dongmei Zhou, Chuanlu Ren, Yubao Cui

{"title":"A digital ELISA for multiplexed detection of allergen‐specific IgE against Der p 1, Der p 2, and Der p 23","authors":"Feifei Han, Shih‐Mo Yang, Ju Xue, Wanying Xie, Yuanfen Liao, Qi Cheng, Dongmei Zhou, Chuanlu Ren, Yubao Cui","doi":"10.1002/btm2.70068","DOIUrl":"https://doi.org/10.1002/btm2.70068","url":null,"abstract":"The house dust mite Dermatophagoides pteronyssinus produces major allergens (Der p 1, Der p 2, and Der p 23) that require precise IgE detection for clinical diagnosis. We developed a multiplex digital ELISA using fluorescence‐encoded micromagnetic beads (532 nm/638 nm dual‐wavelength system) coupled with microfluidics to simultaneously quantify serum IgE against these components, with comprehensive evaluation against the clinical standard UniCAP system. The 532 nm channel measured allergen‐specific signals via average brightness increase (ABMB) of enzymatically amplified fluorescence, while 638 nm enabled spectral bead differentiation. Comparative evaluation with UniCAP showed the improved digital ELISA achieved uniform 75.0% sensitivity but variable specificity (42.9%–54.5%) across allergens at the 15.8% ABMB threshold. Sample classification results (Der p 1: 9 positive/6 negative; Der p 2: 7/8; Der p 23: 7/8) demonstrated suboptimal positive predictive values (33.3%–60.0%) versus more favorable negative predictive values (60.0%–85.7%), with likelihood ratios (LR+: 1.31–1.65) and Cohen's κ (0.12–0.25) suggesting limited diagnostic reliability. The automated platform offered 60% reduced sample volume (20 μL vs. 50 μL), multiplex capability, and maintained sensitivity for low‐titer samples, representing an efficient screening solution pending specificity enhancement.","PeriodicalId":9263,"journal":{"name":"Bioengineering & Translational Medicine","volume":"53 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144915610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Engineered probiotic alleviates ulcerative colitis by inhibiting M1 macrophage polarization via glycolytic reprogramming 工程益生菌通过糖酵解重编程抑制M1巨噬细胞极化，减轻溃疡性结肠炎

IF 7.4 2区医学

Bioengineering & Translational Medicine Pub Date : 2025-08-29 DOI: 10.1002/btm2.70067

Chaoqun Lv, Xinyue Hu, Xiang Li, Wen Shi, Wenbo Li, Yan He, Hongqing Li, Jianxi Bai, Zhenxing Li, Zhipeng Wen, Xinxin Liu, Yuanyuan Ai, Jingchao Li, Xiao Chen, Kaijun Liu

{"title":"Engineered probiotic alleviates ulcerative colitis by inhibiting M1 macrophage polarization via glycolytic reprogramming","authors":"Chaoqun Lv, Xinyue Hu, Xiang Li, Wen Shi, Wenbo Li, Yan He, Hongqing Li, Jianxi Bai, Zhenxing Li, Zhipeng Wen, Xinxin Liu, Yuanyuan Ai, Jingchao Li, Xiao Chen, Kaijun Liu","doi":"10.1002/btm2.70067","DOIUrl":"https://doi.org/10.1002/btm2.70067","url":null,"abstract":"Ulcerative colitis (UC) remains a significant therapeutic challenge due to its complex pathogenesis involving oxidative stress, immune dysregulation, and gut microbiota dysbiosis. Melanin, a natural biopolymer with robust anti‐inflammatory and antioxidant properties, presents a promising treatment avenue for UC. Probiotics, particularly Escherichia coli Nissle 1917 (EcN), have gained recognition for their role in restoring gut homeostasis. In this study, we genetically engineered EcN to overexpress tyrosinase (EcN‐T), facilitating the biosynthesis of melanin specifically for UC treatment. The engineered probiotics demonstrated superior therapeutic efficacy compared to either melanin or EcN administered alone, highlighting a synergistic effect. EcN‐T not only exhibited significant capabilities in scavenging reactive oxygen species and restoring gut microbiota but also possessed the characteristic of enhancing gut colonization time, thereby extending the dosing frequency. Moreover, EcN‐T showcased novel mechanisms, such as the restoration of the intestinal mucosal barrier and the elevation of short‐chain fatty acid levels. Additionally, EcN‐T inhibited M1 macrophage polarization through Hypoxia‐Inducible Factor 1‐alpha (HIF‐1α)dependent glycolytic reprogramming, underscoring its immunomodulatory potential. Collectively, these findings provide new insights into the therapeutic potential of EcN‐T for UC treatment, offering a novel strategy that enhances treatment efficacy while potentially reducing side effects associated with conventional therapies.","PeriodicalId":9263,"journal":{"name":"Bioengineering & Translational Medicine","volume":"27 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144915615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0