Bioengineering & Translational Medicine最新文献_第2页

Establishment of a chemoresistant laryngeal cancer cell model to study chemoresistance and chemosensitization responses via transcriptomic analysis and a tumor‐on‐a‐chip platform 建立化疗耐药喉癌细胞模型，通过转录组学分析和肿瘤芯片平台研究化疗耐药和化疗致敏反应

IF 7.4 2区医学

Bioengineering & Translational Medicine Pub Date : 2025-01-22 DOI: 10.1002/btm2.10741

Christian R. Moya‐Garcia, Meghana Munipalle, Alain Pacis, Nader Sadeghi, Maryam Tabrizian, Nicole Y. K. Li‐Jessen

{"title":"Establishment of a chemoresistant laryngeal cancer cell model to study chemoresistance and chemosensitization responses via transcriptomic analysis and a tumor‐on‐a‐chip platform","authors":"Christian R. Moya‐Garcia, Meghana Munipalle, Alain Pacis, Nader Sadeghi, Maryam Tabrizian, Nicole Y. K. Li‐Jessen","doi":"10.1002/btm2.10741","DOIUrl":"https://doi.org/10.1002/btm2.10741","url":null,"abstract":"Tumor resistance to chemotherapy is a common cause of cancer recurrence in patients with head and neck squamous cell carcinoma. The goal of this study is to establish and characterize a chemoresistant laryngeal cancer cell model and test its potential utility for chemosensitizing therapy. At the genotypic level, RNA sequencing confirmed that the cells acquired putative resistance with upregulated docetaxel‐resistant (DR) genes (e.g., TUBB3, CYP24A1) and signaling pathways (e.g., PI3K/mTOR, autophagy). For phenotypic analysis, DR cells were co‐cultured with laryngeal fibroblasts in a 2‐channel microfluidic chip that mimics a hypoxic tumor core in vivo. A drug sensitivity test with a chemosensitizer, metformin (MTF), was performed on the laryngeal tumor‐on‐a‐chip. Compared to non‐treated controls, MTF‐primed cancer cells exhibit higher sensitivity to docetaxel (DTX), that is, cell death. Collectively, this resistance‐acquired cell model displayed presumed genotypic and phenotypic profiles of chemoresistance providing a viable option for testing new therapeutic strategies for restoring tumor sensitivity to DTX.","PeriodicalId":9263,"journal":{"name":"Bioengineering & Translational Medicine","volume":"24 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143020087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microbead‐based synthetic niches for in vitro expansion and differentiation of human naïve B‐cells 基于微珠的人naïve B细胞体外扩增和分化的合成壁龛

IF 7.4 2区医学

Bioengineering & Translational Medicine Pub Date : 2025-01-17 DOI: 10.1002/btm2.10751

Pearlson Prashanth Austin Suthanthiraraj, Sydney Bone, Kyung‐Ho Roh

{"title":"Microbead‐based synthetic niches for in vitro expansion and differentiation of human naïve B‐cells","authors":"Pearlson Prashanth Austin Suthanthiraraj, Sydney Bone, Kyung‐Ho Roh","doi":"10.1002/btm2.10751","DOIUrl":"https://doi.org/10.1002/btm2.10751","url":null,"abstract":"As the prospect of engineering primary B‐cells for cellular therapies in cancer, autoimmune diseases, and infectious diseases grows, there is an increasing demand for robust in vitro culture systems that effectively activate human B‐cells isolated from peripheral blood for consistent and efficient expansion and differentiation into various effector phenotypes. Feeder cell‐based systems have shown promise in providing long‐term signaling for expanding B‐cells in vitro. However, these co‐culture systems necessitate more rigorous downstream processing to prevent various feeder cell‐related contaminations in the final product, which limits their clinical potential. In this study, we introduce a microbead‐based CD40L‐presentation platform for stable and consistent activation of human naïve B‐cells. By employing a completely synthetic in vitro culture approach integrating B‐cell receptor, CD21 co‐receptor, toll‐like receptor (TLR‐9), and cytokine signals, we demonstrate that naïve B‐cells can differentiate into memory B‐cells (IgD‐CD38‐/lo + CD27+) and antibody‐secreting cells (IgD‐CD38++CD27+). During this process, B‐cells underwent up to a 50‐fold expansion, accompanied by isotype class switching and low levels of somatic hypermutation, mimicking physiological events within the germinal center. The reproducible generation of highly expanded and differentiated effector B‐cells from naïve B‐cells of multiple donors positions this feeder‐free in vitro synthetic niche as a promising platform for large‐scale production of effector B‐cell therapeutics.","PeriodicalId":9263,"journal":{"name":"Bioengineering & Translational Medicine","volume":"7 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142988889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The BAM‐GelMA‐ADSCs bilayer patch promotes tissue regeneration and functional recovery after large‐area bladder defects in beagles BAM - GelMA - ADSCs双分子层贴片促进小猎犬大面积膀胱缺损后的组织再生和功能恢复

IF 7.4 2区医学

Bioengineering & Translational Medicine Pub Date : 2025-01-15 DOI: 10.1002/btm2.10745

Ziyan An, Pengchao Wang, Zhengyun Ling, Kaipeng Bi, Zheng Wang, Jinpeng Shao, Jian Zhao, Zhouyang Fu, Meng Huang, Wenjie Wei, Shuwei Xiao, Jin Zhou, Weijun Fu

{"title":"The BAM‐GelMA‐ADSCs bilayer patch promotes tissue regeneration and functional recovery after large‐area bladder defects in beagles","authors":"Ziyan An, Pengchao Wang, Zhengyun Ling, Kaipeng Bi, Zheng Wang, Jinpeng Shao, Jian Zhao, Zhouyang Fu, Meng Huang, Wenjie Wei, Shuwei Xiao, Jin Zhou, Weijun Fu","doi":"10.1002/btm2.10745","DOIUrl":"https://doi.org/10.1002/btm2.10745","url":null,"abstract":"Previous studies of bladder tissue engineering simply seeded cells onto the surface of the material, which makes the cells lack protection and makes it difficult to face the complex in vivo environment. The gelatin methacryloyl (GelMA) hydrogel possesses outstanding biocompatibility and distinctive photo‐crosslinking characteristics and is capable of offering a suitable three‐dimensional growth environment for cells. This study explored the optimal concentration of GelMA for encapsulating adipose‐derived stem cells (ADSCs) and combined it with bladder acellular matrix (BAM) to create a tissue‐engineered bladder patch. Results indicated that 10% GelMA more effectively promoted ADSCs proliferation and spreading compared to 7.5% and 12.5% concentrations, which can offer a better extracellular matrix environment for cells. BAM performed as an excellent substrate with mechanical properties and stitchability similar to natural tissues. Animal experiments demonstrated that the encapsulated ADSCs in GelMA enhanced patch vascularization in vivo and BAM‐GelMA‐ADSCs tissue‐engineered bladder patch can repair large‐scale bladder defects in beagles and promote bladder tissue regeneration and functional recovery. This photocrosslinking hydrogel‐acellular matrix patch provides a protective semi‐controlled environment for ADSCs, supporting the growth and viability of encapsulated cells in vivo, while being easy to suture and preventing leakage, and has significant clinical potential.","PeriodicalId":9263,"journal":{"name":"Bioengineering & Translational Medicine","volume":"23 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142986004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Convective forces contribute to post‐traumatic degeneration after spinal cord injury 对流力有助于脊髓损伤后的创伤后变性

IF 7.4 2区医学

Bioengineering & Translational Medicine Pub Date : 2025-01-14 DOI: 10.1002/btm2.10739

Hoi Y. Kwon, Christopher Streilein, R. Chase Cornelison

{"title":"Convective forces contribute to post‐traumatic degeneration after spinal cord injury","authors":"Hoi Y. Kwon, Christopher Streilein, R. Chase Cornelison","doi":"10.1002/btm2.10739","DOIUrl":"https://doi.org/10.1002/btm2.10739","url":null,"abstract":"Spinal cord injury (SCI) initiates a complex cascade of chemical and biophysical phenomena that result in tissue swelling, progressive neural degeneration, and formation of a fluid‐filled cavity. Previous studies show fluid pressure above the spinal cord (supraspinal) is elevated for at least 3 days after injury and contributes to a phase of damage called secondary injury. Currently, it is unknown how fluid forces within the spinal cord itself (interstitial) are affected by SCI and if they contribute to secondary injury. We find spinal interstitial pressure increases from −3 mmHg in the naive cord to a peak of 13 mmHg at 3 days post‐injury (DPI) but relatively normalizes to 2 mmHg by 7 DPI. A computational fluid dynamics model predicts interstitial flow velocities up to 0.9 μm/s at 3 DPI, returning to near baseline by 7 DPI. By quantifying vascular leakage of Evans Blue dye after a cervical hemi‐contusion in rats, we confirm an increase in dye infiltration at 3 DPI compared to 7 DPI, suggestive of higher fluid velocities at the time of peak fluid pressure. In vivo expression of the apoptosis marker caspase‐3 is strongly correlated with regions of interstitial flow at 3 DPI, and exogenously enhancing interstitial flow exacerbates tissue damage. In vitro, we show overnight exposure of neuronal cells to low pathological shear stress (0.1 dynes/cm2) significantly reduces cell count and neurite length. Collectively, these results indicate that interstitial fluid flow and shear stress may play a detrimental role in post‐traumatic neural degeneration.","PeriodicalId":9263,"journal":{"name":"Bioengineering & Translational Medicine","volume":"52 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142981182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Artificial intelligence‐enabled innovations in cochlear implant technology: Advancing auditory prosthetics for hearing restoration 人工智能技术在人工耳蜗植入技术中的创新：促进听力修复的听觉修复

IF 7.4 2区医学

Bioengineering & Translational Medicine Pub Date : 2025-01-10 DOI: 10.1002/btm2.10752

Guodao Zhang, Rui Chen, Hamzeh Ghorbani, Wanqing Li, Arsen Minasyan, Yideng Huang, Sen Lin, Minmin Shao

引用次数: 0

Mesenchymal stem cell extracellular vesicle vascularization bioactivity and production yield are responsive to cell culture substrate stiffness 间充质干细胞细胞外囊泡血管化的生物活性和产量与细胞培养基质硬度有关

IF 7.4 2区医学

Bioengineering & Translational Medicine Pub Date : 2025-01-07 DOI: 10.1002/btm2.10743

Emily H. Powsner, Stephanie M. Kronstadt, Kristin Nikolov, Amaya Aranda, Steven M. Jay

{"title":"Mesenchymal stem cell extracellular vesicle vascularization bioactivity and production yield are responsive to cell culture substrate stiffness","authors":"Emily H. Powsner, Stephanie M. Kronstadt, Kristin Nikolov, Amaya Aranda, Steven M. Jay","doi":"10.1002/btm2.10743","DOIUrl":"https://doi.org/10.1002/btm2.10743","url":null,"abstract":"Mesenchymal stem cell‐derived extracellular vesicles (MSC EVs) are an attractive therapeutic option for regenerative medicine applications due to their inherently pro‐angiogenic and anti‐inflammatory properties. However, reproducible and cost‐effective production of highly potent therapeutic MSC EVs is challenging, limiting their translational potential. Here, we investigated whether the well‐characterized responsiveness of MSCs to their mechanical environment—specifically, substrate stiffness—could be exploited to generate EVs with increased therapeutic bioactivity without the need for biochemical priming or genetic manipulation. Using polydimethylsiloxane and bone marrow‐derived MSCs (BM‐MSCs), we show that decreasing the stiffness of MSC substrates to as low as 3 kPa significantly improves the pro‐angiogenic bioactivity of EVs as measured by tube formation and gap closure assays. We also demonstrate that lower substrate stiffness improves EV production and overall yield, important for clinical translation. Furthermore, we establish the mechanoresponsiveness of induced pluripotent stem cell‐derived MSC (iMSC) EVs and their comparability to BM‐MSC EVs, again using tube formation and gap closure assays. With this data, we confirm iMSCs' feasibility as an alternative, renewable cell source for EV production with reduced donor variability. Overall, these results suggest that utilizing substrate stiffness is a promising, simple, and a potentially scalable approach that does not require exogenous cargo or extraneous reagents to generate highly potent pro‐angiogenic MSC EVs.","PeriodicalId":9263,"journal":{"name":"Bioengineering & Translational Medicine","volume":"5 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142935713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ultrasound‐guided interstitial photothermal therapy generates improved treatment responses in a 9464D model of neuroblastoma 超声引导间质光热疗法在9464D神经母细胞瘤模型中产生改善的治疗反应

IF 7.4 2区医学

Bioengineering & Translational Medicine Pub Date : 2025-01-06 DOI: 10.1002/btm2.10749

Grace E. Olsson, Rohan V. Patil, Samantha J. Chin, Katharine N. Rus, Elizabeth E. Sweeney, Karun V. Sharma, Rohan Fernandes

{"title":"Ultrasound‐guided interstitial photothermal therapy generates improved treatment responses in a 9464D model of neuroblastoma","authors":"Grace E. Olsson, Rohan V. Patil, Samantha J. Chin, Katharine N. Rus, Elizabeth E. Sweeney, Karun V. Sharma, Rohan Fernandes","doi":"10.1002/btm2.10749","DOIUrl":"https://doi.org/10.1002/btm2.10749","url":null,"abstract":"We describe the use of ultrasound image guidance to improve treatment outcomes when administering interstitial photothermal therapy (I‐PTT), an experimental cancer treatment modality. I‐PTT is a promising thermal therapy for tumors using intratumorally injected nanoparticle‐based photothermal agents activated by an interstitially placed laser diffuser. We hypothesized that ultrasound‐based image guidance yields improved tumor treatment outcomes in terms of tumor regression and survival by improving the accuracy of the placement of the laser fiber and nanoparticles within a tumor and facilitating more precise PTT delivery. To test this hypothesis, we assessed the effect of ultrasound‐guided I‐PTT (US I‐PTT) on neuroblastoma, an aggressive solid tumor of childhood, using the 9464D syngeneic model in C57BL/6 mice. US I‐PTT using Prussian blue nanoparticles activated by an interstitial cylindrical laser diffuser generated an equivalent in vivo thermal dose as blinded, non‐image‐guided I‐PTT (B I‐PTT). However, US I‐PTT resulted in significantly higher treatment accuracy compared to B I‐PTT, attributable to the image guidance. Importantly, this improved accuracy translated to improved treatment outcomes wherein mice treated with US I‐PTT exhibited significantly improved tumor regression, tumor‐free survival, and long‐term survival compared to mice treated with B I‐PTT. Further, histological analyses of the tumors post‐PTT confirmed the advantages conferred by US I‐PTT over B I‐PTT for tumor control. These proof‐of‐concept results demonstrate the value of using ultrasound guidance for I‐PTT treatment and the translational implications of this approach to provide a more accurate and effective treatment for neuroblastoma.","PeriodicalId":9263,"journal":{"name":"Bioengineering & Translational Medicine","volume":"28 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142934845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mpox disease, diagnosis, and point of care platforms m痘疾病、诊断和护理点平台

IF 7.4 2区医学

Bioengineering & Translational Medicine Pub Date : 2025-01-03 DOI: 10.1002/btm2.10733

Nazente Atceken, Ikra Bayaki, Berk Can, Defne Yigci, Savas Tasoglu

{"title":"Mpox disease, diagnosis, and point of care platforms","authors":"Nazente Atceken, Ikra Bayaki, Berk Can, Defne Yigci, Savas Tasoglu","doi":"10.1002/btm2.10733","DOIUrl":"https://doi.org/10.1002/btm2.10733","url":null,"abstract":"Human Mpox disease (MPX) is an endemic zoonotic disease that develops when patients are infected with the Mpox virus (MPXV). MPXV shares a high level of genetic similarity to other poxviruses and the clinical presentation of MPX is similar to other poxvirus infections which can result in a delay in diagnosis. In addition, the MPXV virus is phylogenetically divided into two different clades which affects the severity of disease. In recent years, there has been an unusual worldwide spread of MPXV, leading to a global public health problem. The most important step in the fight against MPX is rapid, highly specific, and accurate diagnosis. Following the rapid spread of disease in recent years, efforts to develop diagnostic tests have gained momentum. Here, MPX, MPX epidemiology, and MPX diagnostic tests are discussed. Furthermore, biochemical diagnostic tests, molecular diagnostic tests and their development, and point‐of‐care (PoC) diagnostic applications are reviewed. Molecular diagnostic technologies such as polymerase chain reaction, recombinase polymerase amplification, and loop‐mediated isothermal amplification methods that detect MPX are evaluated. Additionally, next‐generation combined molecular techniques and their importance in PoC transition are explored.","PeriodicalId":9263,"journal":{"name":"Bioengineering & Translational Medicine","volume":"1 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142925001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ultrasound‐mediated mechanical forces activate selective tumor cell apoptosis 超声介导的机械力激活选择性肿瘤细胞凋亡

IF 7.4 2区医学

Bioengineering & Translational Medicine Pub Date : 2024-12-31 DOI: 10.1002/btm2.10737

Ajay Tijore, Felix Margadant, Nehal Dwivedi, Leslie Morgan, Mingxi Yao, Anushya Hariharan, Claire Alexandra Zhen Chew, Simon Powell, Glenn Kunnath Bonney, Michael Sheetz

{"title":"Ultrasound‐mediated mechanical forces activate selective tumor cell apoptosis","authors":"Ajay Tijore, Felix Margadant, Nehal Dwivedi, Leslie Morgan, Mingxi Yao, Anushya Hariharan, Claire Alexandra Zhen Chew, Simon Powell, Glenn Kunnath Bonney, Michael Sheetz","doi":"10.1002/btm2.10737","DOIUrl":"https://doi.org/10.1002/btm2.10737","url":null,"abstract":"Recent studies show that tumor cells undergo apoptosis after mechanical stretching, which promotes normal cell growth. Since ultrasound can produce similar sub‐cellular mechanical stresses on the nanoscale, here we test the effect of ultrasound‐mediated mechanical forces on tumors and normal cell survival. Surprisingly, tumor cells undergo apoptosis through a calpain‐dependent mitochondrial pathway that relies upon calcium entry through the mechanosensitive Piezo1 channels. This is a general property of all tumor cell lines tested irrespective of tissue origin, but normal cells are unaffected. In vivo, ultrasound treatment promotes tumor cell killing in a mouse model with invasive CT26 cancer cell subcutaneous tumors and in the chick chorioallantoic membrane (CAM) model with relatively minor damage to chick embryos. Further, patient‐derived pancreatic tumor organoids are killed by ultrasound treatment. Because ultrasound‐mediated mechanical forces cause apoptosis of tumor cells from many different tissues in different microenvironments, it may offer a safe, non‐invasive approach to augment tumor treatments.","PeriodicalId":9263,"journal":{"name":"Bioengineering & Translational Medicine","volume":"33 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142908219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Catalpol promotes the generation of cerebral organoids with oRGs through activation of STAT3 signaling Catalpol通过激活STAT3信号通路促进脑类器官与oRGs的生成

IF 7.4 2区医学

Bioengineering & Translational Medicine Pub Date : 2024-12-30 DOI: 10.1002/btm2.10746

Yoo‐Jung Lee, Byounggook Cho, Daeyeol Kwon, Yunkyung Kim, Saemin An, Soi Kang, Jongpil Kim

{"title":"Catalpol promotes the generation of cerebral organoids with oRGs through activation of STAT3 signaling","authors":"Yoo‐Jung Lee, Byounggook Cho, Daeyeol Kwon, Yunkyung Kim, Saemin An, Soi Kang, Jongpil Kim","doi":"10.1002/btm2.10746","DOIUrl":"https://doi.org/10.1002/btm2.10746","url":null,"abstract":"The generation of human cortical organoids containing outer radial glia (oRG) cells is crucial for modeling neocortical development. Here we show that Catalpol, an iridoid glucoside derived from Rehmannia glutinosa, significantly enhances the generation of cerebral organoids with expanded oRG populations and increased neurogenic potential. Catalpol‐treated organoids exhibited thicker ventricular zone/subventricular zone (VZ/SVZ) and outer subventricular zone (oSVZ) regions, with increased numbers of SOX2 + HOPX+ and SOX2 + TNC+ oRG cells and elevated expression of oRG markers HOPX and FAM107A. We found that Catalpol promoted oRG generation through non‐vertical divisions of ventricular radial glia (vRG) cells, indicating enhanced oRG generation via asymmetrical divisions. Furthermore, we demonstrated that Catalpol augmented oRG cell numbers through activation of the STAT3 signaling pathway. These findings highlight Catalpol's potential in promoting the generation of cerebral organoids with expanded oRG populations and increased neurogenic potential through STAT3 activation, offering new insights into neocortical development modeling.","PeriodicalId":9263,"journal":{"name":"Bioengineering & Translational Medicine","volume":"327 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2024-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142902021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0