Bioengineering & Translational Medicine最新文献_第3页

Correction to “A stretchable, electroconductive tissue adhesive for the treatment of neural injury” 对“治疗神经损伤的可拉伸导电组织胶粘剂”的修正

IF 6.1 2区医学

Bioengineering & Translational Medicine Pub Date : 2025-03-19 DOI: 10.1002/btm2.10759

引用次数: 0

Transport physics‐informed reinforcement learning agents deployed in standalone infusion pumps for managing multidrug delivery in critical care 运输物理信息强化学习代理部署在独立输液泵中，用于管理重症监护中的多药物输送

IF 7.4 2区医学

Bioengineering & Translational Medicine Pub Date : 2025-03-18 DOI: 10.1002/btm2.70013

V. Chandran Suja, A. L. H. S. Detry, N. M. Sims, D. E. Arney, S. Mitragotri, R. A. Peterfreund

{"title":"Transport physics‐informed reinforcement learning agents deployed in standalone infusion pumps for managing multidrug delivery in critical care","authors":"V. Chandran Suja, A. L. H. S. Detry, N. M. Sims, D. E. Arney, S. Mitragotri, R. A. Peterfreund","doi":"10.1002/btm2.70013","DOIUrl":"https://doi.org/10.1002/btm2.70013","url":null,"abstract":"Managing delivery of complex multidrug infusions in anesthesia and critical care presents a significant clinical challenge. Current practices relying on manual control of infusion pumps often result in unpredictable drug delivery profiles and dosing errors—key issues highlighted by the United States Food and Drug Administration (FDA). To address these issues, we introduce the SMART (synchronized‐pump management algorithms for reliable therapies) framework, a novel approach that leverages low Reynolds number drug transport physics and machine learning to accurately manage multidrug infusions in real‐time. SMART is activated based on the Shafer number (), a novel non‐dimensional number that quantifies the relative magnitude of a drug's therapeutic action timescale to its transport timescale within infusion manifolds. SMART is useful when , where drug transport becomes the rate limiting step in achieving the desired therapeutic effects. When activated, SMART monitors multidrug concentrations within infusion manifolds and leverages this information to perform end‐to‐end management of drug delivery using an ensemble of deterministic and deep reinforcement learning (RL) decision networks. Notably, SMART RL networks employ differentially sampled split buffer architecture that accelerates learning and improves performance by seamlessly combining deterministic predictions with RL experience during training. SMART deployed in standalone infusion pumps under simulated clinical conditions outperformed state‐of‐the‐art manual control protocols. This framework has the potential to revolutionize critical care by enhancing accuracy of medication delivery and reducing cognitive workloads. Beyond critical care, the ability to accurately manage multi‐liquid delivery via complex manifolds will have important bearings for manufacturing and process control.","PeriodicalId":9263,"journal":{"name":"Bioengineering & Translational Medicine","volume":"55 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143653389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to “Drug cross-linking electrospun fiber for effective infected wound healing” 更正“药物交联静电纺丝纤维有效治疗感染伤口”

IF 6.1 2区医学

Bioengineering & Translational Medicine Pub Date : 2025-03-17 DOI: 10.1002/btm2.10758

引用次数: 0

Paquinimod‐hydrogel hybrid microneedle array patch alleviates hypertrophic scar via inhibiting M1 polarization 帕喹尼莫德-水凝胶混合微针阵列贴片通过抑制M1极化减轻增生性疤痕

IF 7.4 2区医学

Bioengineering & Translational Medicine Pub Date : 2025-03-15 DOI: 10.1002/btm2.70016

Zihui Zhang, Peng Wang, Hengdeng Liu, Hanwen Wang, Miao Zhen, Xuefeng He, Suyue Gao, Juntao Xie, Julin Xie

{"title":"Paquinimod‐hydrogel hybrid microneedle array patch alleviates hypertrophic scar via inhibiting M1 polarization","authors":"Zihui Zhang, Peng Wang, Hengdeng Liu, Hanwen Wang, Miao Zhen, Xuefeng He, Suyue Gao, Juntao Xie, Julin Xie","doi":"10.1002/btm2.70016","DOIUrl":"https://doi.org/10.1002/btm2.70016","url":null,"abstract":"Hypertrophic scar (HS) is one of the most common complications of skin injuries, with a lack of effective therapeutic approaches to date. Most current research has focused on the dysfunction of hypertrophic scar fibroblasts (HSFBs) and dermal vascular endothelial cells (HDVECs), neglecting the crucial role of the inflammatory microenvironment that causes them to be abnormal. In this study, we first discovered and validated that the S100A8/9 specific inhibitor Paquinimod could inhibit macrophage polarization toward M1, and further suppress the proliferation, migration, collagen formation, and angiogenesis of HSFBs and HDVECs in vitro. This mechanism has also been validated in a rat model of HS. Then, we developed a good biocompatibility and penetrability Paquinimod‐Hydrogel Hybrid Microneedle Array Patch (PHMAP) for HS treatment. With the advantages of excellent penetrability, surface sealing, sustained release, and precise uniform distribution, PHMAP exhibited superior therapeutic efficacy over intravenous and intradermal injections. These results suggest that PHMAP can be a promising and advanced solution for HS prevention and therapies.","PeriodicalId":9263,"journal":{"name":"Bioengineering & Translational Medicine","volume":"19 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143631323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synergistic drug combination screening using a nanodroplet processing platform to enhance neuroblastoma treatment in TH‐MYCN transgenic mice 利用纳米微滴处理平台进行协同药物联合筛选以增强TH‐MYCN转基因小鼠的神经母细胞瘤治疗

IF 7.4 2区医学

Bioengineering & Translational Medicine Pub Date : 2025-03-12 DOI: 10.1002/btm2.70007

Yen‐Tzu Liao, Zhi‐Kai Yu, Yi‐Xun Huang, Kuan‐Hung Lin, Ching‐Te Kuo, Tsai‐Shan Yang, Pei‐Yi Wu, Chi‐Tai Yeh, Yen‐Lin Liu, Chien‐Chin Chen, Chiung‐Nien Chen, Wen‐Ming Hsu, Hsinyu Lee

{"title":"Synergistic drug combination screening using a nanodroplet processing platform to enhance neuroblastoma treatment in TH‐MYCN transgenic mice","authors":"Yen‐Tzu Liao, Zhi‐Kai Yu, Yi‐Xun Huang, Kuan‐Hung Lin, Ching‐Te Kuo, Tsai‐Shan Yang, Pei‐Yi Wu, Chi‐Tai Yeh, Yen‐Lin Liu, Chien‐Chin Chen, Chiung‐Nien Chen, Wen‐Ming Hsu, Hsinyu Lee","doi":"10.1002/btm2.70007","DOIUrl":"https://doi.org/10.1002/btm2.70007","url":null,"abstract":"Neuroblastoma is a highly aggressive pediatric cancer with a poor prognosis, particularly in high‐risk (HR) cases characterized by MYCN amplification. The severe side effects associated with high‐dose chemotherapy further complicate treatment. Despite significant advancements in drug screening, traditional platforms remain limited due to their requirement for large cell quantities and their low translational success from bench to clinic. These limitations hinder the application of personalized medicine screening for patients with neuroblastoma. To address these challenges, we developed a Bioinspired Nanodroplet Processing (BioNDP) platform. This innovative platform allows for the simultaneous screening of multiple drug combinations while reducing the required number of cells to just 100 and minimizing assay volumes to 200 nL per well. Using BioNDP, we screened chemotherapeutic combinations of cyclophosphamide, doxorubicin, and vincristine in both the SK‐N‐DZ neuroblastoma cell line and primary neuroblastoma cells derived from TH‐MYCN transgenic mice. Our findings revealed a specific drug combination that exhibited significant synergistic cytotoxicity in neuroblastoma cells. This combination completely eradicated tumors and significantly improved survival rates in TH‐MYCN mice, without notable side effects. This study highlights the potential of the BioNDP platform in bridging in vitro and in vivo results, offering a promising strategy for personalized medicine in the treatment of HR neuroblastoma, with reduced toxicity and enhanced therapeutic efficacy.","PeriodicalId":9263,"journal":{"name":"Bioengineering & Translational Medicine","volume":"13 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143607892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In vivo evaluation of decellularized skeletal muscle matrices for skeletal muscle repair: A systematic review 去细胞骨骼肌基质用于骨骼肌修复的体内评价：系统综述

IF 7.4 2区医学

Bioengineering & Translational Medicine Pub Date : 2025-03-12 DOI: 10.1002/btm2.70009

Ina Hennion, Charlot Philips, Chong Jiang, Nele Van De Winkel, Laurens J. Ceulemans, Lieven Thorrez

{"title":"In vivo evaluation of decellularized skeletal muscle matrices for skeletal muscle repair: A systematic review","authors":"Ina Hennion, Charlot Philips, Chong Jiang, Nele Van De Winkel, Laurens J. Ceulemans, Lieven Thorrez","doi":"10.1002/btm2.70009","DOIUrl":"https://doi.org/10.1002/btm2.70009","url":null,"abstract":"Volumetric muscle loss is the significant loss of skeletal muscle volume beyond the innate regenerative capacity, resulting in functional impairment. The current standard of care combines muscle autografting with physical therapy but is often insufficient to reach full recovery. Decellularized skeletal muscle (DSM) provides an interesting alternative to repair volumetric muscle loss. The native structure and composition of the extracellular matrix in these acellular implants provide a blueprint for muscle regeneration. Moreover, DSM can be combined with cells to facilitate the regeneration of the skeletal muscle defect. This systematic review provides a complete and thorough overview of the state‐of‐the‐art applications and efficacy of DSM matrices in skeletal muscle repair in vivo, selected according to the Preferred Reporting Items for Systematic Reviews and Meta‐Analyses guidelines. Technical information on the different methods to create DSM implants and the implantation studies is provided. Moreover, details on the evaluation of the structural and functional regeneration of the muscle defect after implantation of the DSM are described. Results reveal a large heterogeneity in the analysis of regeneration upon DSM implantation. This heterogeneity makes it difficult to fully assess the efficiency of DSM to regenerate skeletal muscle, hampering further translation of this technique. Therefore, we suggest a multi‐level evaluation method to assess (i) muscle regeneration, (ii) vascularization, (iii) innervation of the regenerated muscle, and (iv) functional regeneration in a quantitative way.","PeriodicalId":9263,"journal":{"name":"Bioengineering & Translational Medicine","volume":"32 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143607890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Micrometer‐scale tPA beads amplify plasmin generation for enhanced thrombolytic therapy 微米级tPA珠可扩增纤溶蛋白的产生，增强溶栓治疗

IF 7.4 2区医学

Bioengineering & Translational Medicine Pub Date : 2025-03-12 DOI: 10.1002/btm2.70012

Matthew J. Osmond, Fabrice Dabertrand, Nidia Quillinan, Enming J. Su, Daniel A. Lawrence, David W. M. Marr, Keith B. Neeves

{"title":"Micrometer‐scale tPA beads amplify plasmin generation for enhanced thrombolytic therapy","authors":"Matthew J. Osmond, Fabrice Dabertrand, Nidia Quillinan, Enming J. Su, Daniel A. Lawrence, David W. M. Marr, Keith B. Neeves","doi":"10.1002/btm2.70012","DOIUrl":"https://doi.org/10.1002/btm2.70012","url":null,"abstract":"Rapid restoration of blood flow is critical in treating acute ischemic stroke. Current thrombolytic therapies using tissue plasminogen activator (tPA) are limited by low recanalization rates and risks of off‐target bleeding. Here, we demonstrate that a remarkably simple adjustment—using micrometer‐scale rather than sub‐micrometer particles to immobilize tPA—fundamentally improves thrombolysis. By merely increasing the particle diameter from 0.1 to 1.0 μm, we achieve a dramatic shift in lysis dynamics: 1.0 μm tPA‐beads generate higher plasmin flux, readily overcome antiplasmin inhibition, and trigger a self‐propagating cascade of fibrinolysis. This leads to near‐complete clot dissolution at tPA doses nearly 100‐fold lower than standard free tPA, both in vitro and in a murine model of acute ischemic stroke. Within minutes, low‐dose 1.0 μm tPA beads fully restore blood flow, outperforming conventional therapies. Our results show that simply scaling up particle size can resolve kinetic and transport barriers in thrombolysis, offering a promising advancement in stroke treatment with potential applications in other thrombotic disorders.","PeriodicalId":9263,"journal":{"name":"Bioengineering & Translational Medicine","volume":"54 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143607889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Engineered microvascular basement membrane mimetic for real‐time neutrophil tracking in the microvascular wall 用于微血管壁中性粒细胞实时跟踪的工程微血管基膜模拟物

IF 7.4 2区医学

Bioengineering & Translational Medicine Pub Date : 2025-03-12 DOI: 10.1002/btm2.70008

Laura C. Morales, Catherine D. Kim, Yangang Pan, Simon Scheuring, Anjelica L. Gonzalez

{"title":"Engineered microvascular basement membrane mimetic for real‐time neutrophil tracking in the microvascular wall","authors":"Laura C. Morales, Catherine D. Kim, Yangang Pan, Simon Scheuring, Anjelica L. Gonzalez","doi":"10.1002/btm2.70008","DOIUrl":"https://doi.org/10.1002/btm2.70008","url":null,"abstract":"The microvascular basement membrane (mvBM) is crucial in maintaining vascular integrity and function and, therefore, key to the health of major organs. However, the complex nature and the intricate interplay of biochemical and biomechanical factors that regulate the mvBM functional dynamics make it difficult to study. Here, we present a novel and highly tunable in vitro model of the human mvBM, enabling a bottom‐up approach to assemble a composite model of the microvascular wall and explore microvascular dynamics and interactions with circulating neutrophils in real time. An electrospun polyethylene glycol (PEG)‐based fibrillar network mimics the mvBM with adjustable nanofiber diameter, orientation, and density. The fidelity of the model to the human mvBM's topography and mechanics was verified through second harmonic generation imaging and atomic force microscopy. PEG was functionalized with bioactive moieties to enable endothelial cell (EC) and pericyte (PC) attachment, through which neutrophil interactions with the microvascular wall model were observed. The model, coupled with 4D microscopy, revealed nuanced and dynamic neutrophil behavior when interacting with the microvascular wall, demonstrating its utility in characterizing cell–cell interactions. As such, the model can be employed in the exploration of inflammatory and microvascular‐related diseases. Therefore, this innovative approach represents a significant advancement in vascular biology research, holding profound implications for understanding mvBM dynamics in both health and disease.","PeriodicalId":9263,"journal":{"name":"Bioengineering & Translational Medicine","volume":"5 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143607891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bisulfite‐free PCDHGB7 methylation in urine enables early noninvasive detection of urothelial carcinoma 尿中不含亚硫酸氢盐的PCDHGB7甲基化可以早期无创检测尿路上皮癌

IF 7.4 2区医学

Bioengineering & Translational Medicine Pub Date : 2025-03-11 DOI: 10.1002/btm2.70004

Zhicong Yang, Qing Chen, Shihua Dong, Peng Xu, Zhanrui Mao, Yaping Dong, Wei Li, Wenxuan Li, Yang Han, Lihe Dai, Gehong Dong, Yong Zhang, Yinshan Li, Liang Cheng, Weimin Ci, Wenqiang Yu, Chuanliang Xu

{"title":"Bisulfite‐free PCDHGB7 methylation in urine enables early noninvasive detection of urothelial carcinoma","authors":"Zhicong Yang, Qing Chen, Shihua Dong, Peng Xu, Zhanrui Mao, Yaping Dong, Wei Li, Wenxuan Li, Yang Han, Lihe Dai, Gehong Dong, Yong Zhang, Yinshan Li, Liang Cheng, Weimin Ci, Wenqiang Yu, Chuanliang Xu","doi":"10.1002/btm2.70004","DOIUrl":"https://doi.org/10.1002/btm2.70004","url":null,"abstract":"Urothelial carcinomas (UCs) are the fourth most common male malignancies. However, currently implemented detection methods for UC are usually invasive and/or show passable sensitivity and specificity. An accurate, practical, and effective approach is urgently needed for UC clinical detection. Based on the observation that PCDHGB7 was hypermethylated in UC, we developed a bisulfite‐free quantitative polymerase chain reaction (qPCR)‐based PCDHGB7 evaluation to enable urine for UC noninvasive detection. A total of 887 urine samples from UC/benign diseases of the urinary system (BUD) patients between 2022 and 2023 were included. All collected samples were divided into training and validation sets in a 2:1 ratio based on the order of patient enrollment. Results showed that hypermethylated PCDHGB7 exhibited excellent sensitivity of 87.3% (95% CI: 80.7%–92.3%) and specificity of 91.0% (95% CI: 84.8%–95.3%) in efficiently distinguishing UC from BUD patients in the validation set, which is highly consistent with its performance in the training set. Moreover, PCDHGB7 hypermethylation showed promising potential in identifying sessile UC tumors or cases that might be missed in clinical detection and outperformed standard urine cytology in detecting bladder cancer (82.1% vs. 34.5%), ureter cancer (78.1% vs. 34.4%), and renal pelvis cancer (90.9% vs. 22.7%). Overall, bisulfite‐free qPCR‐based PCDHGB7 evaluation in urine provided a noninvasive, easy‐to‐perform, and effective way for UC early detection.","PeriodicalId":9263,"journal":{"name":"Bioengineering & Translational Medicine","volume":"87 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143599488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Capture of Group A Streptococcus by open‐microfluidic CandyCollect device in pediatric patients 开放微流控CandyCollect装置在儿科患者中捕获A群链球菌

IF 7.4 2区医学

Bioengineering & Translational Medicine Pub Date : 2025-03-04 DOI: 10.1002/btm2.70001

Wan‐chen Tu, Ingrid H. Robertson, Andrea Blom, Elena Alfaro, Victoria A. M. Shinkawa, Daniel B. Hatchett, Juan C. Sanchez, Anika M. McManamen, Xiaojing Su, Erwin Berthier, Sanitta Thongpang, Ellen R. Wald, Gregory P. DeMuri, Ashleigh B. Theberge

{"title":"Capture of Group A Streptococcus by open‐microfluidic CandyCollect device in pediatric patients","authors":"Wan‐chen Tu, Ingrid H. Robertson, Andrea Blom, Elena Alfaro, Victoria A. M. Shinkawa, Daniel B. Hatchett, Juan C. Sanchez, Anika M. McManamen, Xiaojing Su, Erwin Berthier, Sanitta Thongpang, Ellen R. Wald, Gregory P. DeMuri, Ashleigh B. Theberge","doi":"10.1002/btm2.70001","DOIUrl":"https://doi.org/10.1002/btm2.70001","url":null,"abstract":"State the PurposeObtaining high‐quality samples to diagnose streptococcal pharyngitis in pediatric patients is challenging due to discomfort associated with traditional pharyngeal swabs. This may cause reluctance to go to the clinic, inaccurate diagnosis, or inappropriate treatment for children with sore throats. Here, we determined the efficacy of CandyCollect, a lollipop‐inspired open‐microfluidic pathogen collection device, to capture Group A Streptococcus (GAS) and compare user preference for CandyCollect, conventional pharyngeal swabs, or mouth swabs in children with pharyngitis and their caregivers.ResultsAll child participants (30/30) were positive for GAS by qPCR on both the mouth swab and CandyCollect. Caregivers ranked CandyCollect as a good sampling method overall (27/30), and all caregivers (30/30) would recommend CandyCollect for children 5 years and older. Twenty‐three of 30 children “really like” the taste and 24/30 would prefer to use CandyCollect if a future test were needed. All caregivers (30/30) and most children (28/30) would be willing to use CandyCollect at home.ConclusionAll participants tested positive for GAS on all three collection methods (pharyngeal swab, mouth swab, and CandyCollect). While both caregivers and children like CandyCollect, some caregivers would prefer a shorter collection time. Future work includes additional studies with larger cohorts presenting with pharyngitis of unknown etiology and shortening collection time while maintaining the attractive form of the device.","PeriodicalId":9263,"journal":{"name":"Bioengineering & Translational Medicine","volume":"6 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143546144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0