Proceedings of the West Virginia Academy of Science最新文献

Generation and comparison of broad-host range inducible expression vectors for use in Gram-negative bacteria including ESKAPE pathogens. 用于革兰氏阴性菌包括ESKAPE病原体的广谱诱导表达载体的生成和比较。

Proceedings of the West Virginia Academy of Science Pub Date : 2025-01-01 Epub Date: 2025-05-01

Raj K Ginde, Nicholas A Stella, Rachel C Calvario, Kara M Lehner, Jake D Callaghan, Daniel R Komlosi, Joseph Horzempa, Robert M Q Shanks

{"title":"Generation and comparison of broad-host range inducible expression vectors for use in Gram-negative bacteria including ESKAPE pathogens.","authors":"Raj K Ginde, Nicholas A Stella, Rachel C Calvario, Kara M Lehner, Jake D Callaghan, Daniel R Komlosi, Joseph Horzempa, Robert M Q Shanks","doi":"","DOIUrl":"","url":null,"abstract":"The objective of this study is a controlled comparison of several carbohydrate inducible promoter in important bacterial species. Inducible promoter systems are invaluable for biotechnology and basic science applications. However, few inducible promoters are available on plasmids that replicate in Saccharomyces cerevisiae, which enables gap-repair recombination, or on broad host-range vectors, which allows replication in many Gram-negative genera. In this study we generated shuttle vectors with S. cerevisiae and the broad host range pBBR1 bacterial replicon. These contained a variety of inducible promoters and used a highly sensitive luxCDABE reporter in Acinetobacter baumannii, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Pseudomonas fluorescens, and Serratia marcescens. Tested carbohydrate-inducible promoters were P BAD , P rhaB , P T5 , and P xut , isolated from A. baumannii. In the Enterobacterales the P BAD and P rhaB promoters demonstrated the highest levels of inducibility at 100- and 5,600-fold, respectively. For P. aeruginosa P rhaB and P xut were the most inducible at 40- and 10-fold, respectively. For P. fluorescens all of the carbohydrate-inducible promoters had similar inducibility, under 10-fold. None of the carbohydrate-inducible promoters were effective for A. baumannii; however, they could be used as constitutive promoters. Data indicated that the rhamnose-inducible promoter excelled among the carbohydrate-inducible promoters for most tested organisms, and this study highlights the need for better inducible promoters for A. baumannii.","PeriodicalId":92280,"journal":{"name":"Proceedings of the West Virginia Academy of Science","volume":"97 1","pages":"58-69"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12352128/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144877209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Symmetry Equivalents of the Weak Value Measurement Pointer Hamiltonian 弱值测量指针哈密顿的对称等价物

Proceedings of the West Virginia Academy of Science Pub Date : 2024-06-04 DOI: 10.55632/pwvas.v96i2.1100

Allen Parks

引用次数: 0

West Virginia Human Whole-Body Donors in Undergraduate Biology Education at Radford University 西弗吉尼亚州人体全身捐献者在拉德福德大学生物学本科生教育中的应用

Proceedings of the West Virginia Academy of Science Pub Date : 2024-06-03 DOI: 10.55632/pwvas.v95i1.1023

Laura Gruss, Matthew Close, Sara O'Brien

引用次数: 0

Geographical Impact of Human Gift Registries in West Virginia: A Model for Centralized Resources in Human Anatomy Education 西弗吉尼亚州人体馈赠登记处的地域影响：人体解剖学教育的集中资源模式

Proceedings of the West Virginia Academy of Science Pub Date : 2024-02-07 DOI: 10.55632/pwvas.v95i1.1024

David V. Rasicci, Obadah Tolaymat, Robert J. Bolyard, Christopher A. Dunmore, Jake T. Neumann, M. Zdilla, H. Wayne Lambert

{"title":"Geographical Impact of Human Gift Registries in West Virginia: A Model for Centralized Resources in Human Anatomy Education","authors":"David V. Rasicci, Obadah Tolaymat, Robert J. Bolyard, Christopher A. Dunmore, Jake T. Neumann, M. Zdilla, H. Wayne Lambert","doi":"10.55632/pwvas.v95i1.1024","DOIUrl":"https://doi.org/10.55632/pwvas.v95i1.1024","url":null,"abstract":"The West Virginia State Anatomical Board oversees three Human Gift Registries (HGRs), which allocate human whole body donations to support the educational, outreach, and research missions of our universities. These HGRs primarily function to meet the demand for anatomy education at the institutions that house them. Two of these HGRs, however, demonstrate a unique centralized model, in which approximately half of the donors are reallocated to other institutions to support their missions, and thus, have an impact far greater than in West Virginia (WV) alone. In this perspective, the number of donations received at WV HGRs over the past seven years are reported by institution, as well as the geographical distribution of our donors over the past five years. We also discuss the impact of the COVID-19 pandemic on human anatomy education. This model of centralized HGRs in WV may be of interest to other State Anatomical Boards, as well as the families of past and prospective donors in WV. The state of WV remains committed to dissection-based human anatomy education, and we are forever grateful to our donors who make this experience possible.","PeriodicalId":92280,"journal":{"name":"Proceedings of the West Virginia Academy of Science","volume":"25 5‐6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139796590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Geographical Impact of Human Gift Registries in West Virginia: A Model for Centralized Resources in Human Anatomy Education 西弗吉尼亚州人体馈赠登记处的地域影响：人体解剖学教育的集中资源模式

Proceedings of the West Virginia Academy of Science Pub Date : 2024-02-07 DOI: 10.55632/pwvas.v95i1.1024

David V. Rasicci, Obadah Tolaymat, Robert J. Bolyard, Christopher A. Dunmore, Jake T. Neumann, M. Zdilla, H. Wayne Lambert

{"title":"Geographical Impact of Human Gift Registries in West Virginia: A Model for Centralized Resources in Human Anatomy Education","authors":"David V. Rasicci, Obadah Tolaymat, Robert J. Bolyard, Christopher A. Dunmore, Jake T. Neumann, M. Zdilla, H. Wayne Lambert","doi":"10.55632/pwvas.v95i1.1024","DOIUrl":"https://doi.org/10.55632/pwvas.v95i1.1024","url":null,"abstract":"The West Virginia State Anatomical Board oversees three Human Gift Registries (HGRs), which allocate human whole body donations to support the educational, outreach, and research missions of our universities. These HGRs primarily function to meet the demand for anatomy education at the institutions that house them. Two of these HGRs, however, demonstrate a unique centralized model, in which approximately half of the donors are reallocated to other institutions to support their missions, and thus, have an impact far greater than in West Virginia (WV) alone. In this perspective, the number of donations received at WV HGRs over the past seven years are reported by institution, as well as the geographical distribution of our donors over the past five years. We also discuss the impact of the COVID-19 pandemic on human anatomy education. This model of centralized HGRs in WV may be of interest to other State Anatomical Boards, as well as the families of past and prospective donors in WV. The state of WV remains committed to dissection-based human anatomy education, and we are forever grateful to our donors who make this experience possible.","PeriodicalId":92280,"journal":{"name":"Proceedings of the West Virginia Academy of Science","volume":"23 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139856395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of Teaching Nutritional Biochemistry Concepts Using Pop Culture: Why Zombies Crave Brains 利用流行文化教授营养生物化学概念的影响：僵尸为何渴望大脑

Proceedings of the West Virginia Academy of Science Pub Date : 2023-12-28 DOI: 10.55632/pwvas.v95i1.944

Kristy Henson, G. Popovich

{"title":"Impact of Teaching Nutritional Biochemistry Concepts Using Pop Culture: Why Zombies Crave Brains","authors":"Kristy Henson, G. Popovich","doi":"10.55632/pwvas.v95i1.944","DOIUrl":"https://doi.org/10.55632/pwvas.v95i1.944","url":null,"abstract":"In recent years, the allure of zombie culture has found its way into innovative teaching of biology and medical courses. In this current example, we employ zombie imagery and context to facilitate an understanding of applied nutritional biochemistry. This novel approach is compared to teaching identical material using traditional examples and context. Specifically, we investigated the impact on short-term and long-term learning by incorporating zombie lore into the delivery of a lecture on the inflammatory cascade as it relates to essential fatty acids. We hypothesized that given the popularity of zombies and dystopian/apocalyptic environments, intertwining nutritional concepts with these dramatic scenarios would enhance interest and attention, hence improving learning. Two presentations were offered in basic nutrition (synchronous) or introductory anatomy and physiology (asynchronous). This audience was selected to ensure minimal baseline knowledge on the topic of essential fatty acids. Half of the students received a non-zombie lecture while half received the same information as it relates to zombies. Learning was assessed using a five-question survey. Testing was conducted before, immediately following, and 4 weeks after the lecture. The mean score increase pre- versus post-test in both groups was dramatic but not significantly different between groups (zombie vs non-zombie and synchronous vs asynchronous). Results were as follows: the pre-test average for both groups was 42.37%; the immediate post-test average was 85.96%. At 4 weeks, the average performance dropped to 70.30%. On average, students indicated a mild interest in zombies. In this instance, teaching with pop culture did not significantly impact short-term or long-term retention.","PeriodicalId":92280,"journal":{"name":"Proceedings of the West Virginia Academy of Science","volume":"29 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139149732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Right Ectopic Pelvic Kidney and Bilateral Vascular Aberrations a Female Donor: A Case Report 右侧异位盆腔肾脏和双侧血管畸形，女性捐献者：病例报告

Proceedings of the West Virginia Academy of Science Pub Date : 2023-12-28 DOI: 10.55632/pwvas.v95i1.1026

H. S. Kieffer, Samuel Zercher, Silas Buckwalter

引用次数: 0

Scoping Review of Wearables in Monitoring Opioid, Tobacco, and Alcohol Abuse: A Potential Intervention for West Virginia 可穿戴设备在监测阿片类药物、烟草和酒精滥用方面的范围审查：西弗吉尼亚州的潜在干预措施

Proceedings of the West Virginia Academy of Science Pub Date : 2023-12-28 DOI: 10.55632/pwvas.v95i3.1025

Oyekunle Shopeju, Avishek Choudhury

{"title":"Scoping Review of Wearables in Monitoring Opioid, Tobacco, and Alcohol Abuse: A Potential Intervention for West Virginia","authors":"Oyekunle Shopeju, Avishek Choudhury","doi":"10.55632/pwvas.v95i3.1025","DOIUrl":"https://doi.org/10.55632/pwvas.v95i3.1025","url":null,"abstract":"Wearable technology is a useful tool to tackle the problem of substance abuse among populations by tracking and monitoring addictions and offering diagnosis by proxy. This review seeks to understand the role of wearable technology in managing addiction, with particular focus on opioid, alcohol, and tobacco abuse. We adhered to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses extension for Scoping Reviews (PRISMA-ScR) guidelines and conducted a comprehensive search of PubMed, PubMed Central, Web of Science (WoS), and IEEE Explore databases. English-language peer-reviewed articles published between 2013 and 2022 that examined the use of wearable devices for addiction management were included. The review acknowledged 25 relevant studies from various countries, primarily the United States. A majority of the studies examined alcohol addiction, with Secure Continuous Remote Alcohol Monitor (SCRAM) being the most commonly used technology. Our findings highlight the positive impact of wearables such as Q sensor™ Affectiva, Spire Health Tag, and Empatica E4 sensors on monitoring and managing opioid addiction, including their potential in addressing the opioid crisis in the United States. Moreover, the data underscored the importance of wearable technology in studying smoking patterns, with devices like (Automated Smoking Perception and Recording) ASPIRE and (Personal Automatic Cigarette Tracker) PACT 2.0 showing high agreement with other indicators of smoking characteristics. Despite the progress made, the review identifies a need for more research into wearables for opioid addiction.","PeriodicalId":92280,"journal":{"name":"Proceedings of the West Virginia Academy of Science","volume":"274 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139152725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of sample collection containers for selenium quantification 评估用于硒定量的样品采集容器

Proceedings of the West Virginia Academy of Science Pub Date : 2023-12-28 DOI: 10.55632/pwvas.v95i3.1019

Rebecca K. McGrail, Donglin Huang, Dorothy Vesper, Louis McDonald

引用次数: 0

The effect of fat distribution and nutritional behavior on balance and equilibrium of acrobatics and tumbling athletes 脂肪分布和营养行为对杂技和翻滚运动员平衡性的影响

Proceedings of the West Virginia Academy of Science Pub Date : 2023-09-15 DOI: 10.55632/pwvas.v95i1.943

Priscilla Richmond, Jiaya Zhu, Taylor Kennedy, Chloe L. Sindledecker, Kristie Kiefer, Andrea Haney, Julia Matzenbacher dos Santos

{"title":"The effect of fat distribution and nutritional behavior on balance and equilibrium of acrobatics and tumbling athletes","authors":"Priscilla Richmond, Jiaya Zhu, Taylor Kennedy, Chloe L. Sindledecker, Kristie Kiefer, Andrea Haney, Julia Matzenbacher dos Santos","doi":"10.55632/pwvas.v95i1.943","DOIUrl":"https://doi.org/10.55632/pwvas.v95i1.943","url":null,"abstract":"Acrobatics and Tumbling (A&T) is an emerging sport in the United States; balance and equilibrium are critical components of performance in this sport. Therefore, the aim of this study was to identify if body composition, fat distribution, and nutritional behavior affect the balance and vertical jump of A&T athletes. Body mass index (BMI), whole body percentage of fat and skeletal muscle, and fat distribution of top and base A&T athletes from Fairmont State University were assessed using an impedance device (In Body 570). Nutritional behavior was assessed by a 3-day food log. Balance was measured by the time of holding a handstand in seconds. BMI, whole body, and truncal fat percentage were higher in base athletes when compared to tops (p˂0.05). When associating balance with the variables, skeletal muscle mass was positively associated with balance (r=0.49, p˂0.05), and the percentage of fat and fat accumulation in the lower limbs were negatively associated (r=-0.47 and -0.48, respectively p˂0.05). When the association was tested within the position, the percentage of skeletal muscle mass and fat was only associated with balance in top athletes. Thus, training strategies to increment skeletal muscle mass and decrease body fat should be the focus to improve the balance of top A&T athletes.","PeriodicalId":92280,"journal":{"name":"Proceedings of the West Virginia Academy of Science","volume":"5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135395884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0