BioseparationPub Date : 2000-01-01DOI: 10.1023/A:1011190713820
A. Rodenbrock, K. Selber, M. Egmond, M. Kula
{"title":"Extraction of peptide tagged cutinase in detergent-based aqueous two-phase systems","authors":"A. Rodenbrock, K. Selber, M. Egmond, M. Kula","doi":"10.1023/A:1011190713820","DOIUrl":"https://doi.org/10.1023/A:1011190713820","url":null,"abstract":"","PeriodicalId":9179,"journal":{"name":"Bioseparation","volume":"61 1","pages":"269-276"},"PeriodicalIF":0.0,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83870184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BioseparationPub Date : 2000-01-01DOI: 10.1023/A:1008133914854
L. Trinh, S. Noronha, M. Fannon, J. Shiloach
{"title":"Recovery of mouse endostatin produced by Pichia pastoris using expanded bed adsorption","authors":"L. Trinh, S. Noronha, M. Fannon, J. Shiloach","doi":"10.1023/A:1008133914854","DOIUrl":"https://doi.org/10.1023/A:1008133914854","url":null,"abstract":"","PeriodicalId":9179,"journal":{"name":"Bioseparation","volume":"30 1","pages":"223-230"},"PeriodicalIF":0.0,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73731442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BioseparationPub Date : 2000-01-01DOI: 10.1023/A:1011194324047
U. Gündüz
{"title":"Optimization of bovine serum albumin partition coefficient in aqueous two-phase systems","authors":"U. Gündüz","doi":"10.1023/A:1011194324047","DOIUrl":"https://doi.org/10.1023/A:1011194324047","url":null,"abstract":"","PeriodicalId":9179,"journal":{"name":"Bioseparation","volume":"124 1","pages":"277-281"},"PeriodicalIF":0.0,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74682039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BioseparationPub Date : 2000-01-01DOI: 10.1023/a:1008183828223
S Jarudilokkul, E Paulsen, D C Stuckey
{"title":"The effect of demulsifiers on lysozyme extraction from hen egg white using reverse micelles.","authors":"S Jarudilokkul, E Paulsen, D C Stuckey","doi":"10.1023/a:1008183828223","DOIUrl":"https://doi.org/10.1023/a:1008183828223","url":null,"abstract":"<p><p>The liquid-liquid extraction of protein from buffered aqueous phases using reverse micelles (RM) has been extensively researched from a fundamental point of view. However, very little effort has been expended at scaling up this process for the extraction of real fermentation broth. When real broths are used with reverse micellar phases there are major problems with emulsion formation. In this study the effect of a variety of demulsifiers on lysozyme extraction was evaluated in terms of their influence on the separating properties of the emulsion, water content (Wo), and, extraction yield and kinetics from both buffer and hen egg white. In addition, the use of a low shear contactor (a Graesser or 'raining bucket') was assessed in terms of its suitability as a RM contactor. It was found that most of the demulsifiers reduced the settling time of the emulsion, and enhanced the yield and kinetics of lysozyme extraction from hen egg white. It was hypothesised that this was due to the demulsifier displacing the lysozyme from the interface and preventing the protein unfolding and precipitating. This effect was found to depend on both the generic type of demulsifier, and its concentration.</p>","PeriodicalId":9179,"journal":{"name":"Bioseparation","volume":"9 2","pages":"81-91"},"PeriodicalIF":0.0,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1023/a:1008183828223","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21734411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BioseparationPub Date : 2000-01-01DOI: 10.1023/a:1008121005974
S Sharma, A Sharma, M N Gupta
{"title":"One step purification of peanut phospholipase D by precipitation with alginate.","authors":"S Sharma, A Sharma, M N Gupta","doi":"10.1023/a:1008121005974","DOIUrl":"https://doi.org/10.1023/a:1008121005974","url":null,"abstract":"<p><p>A simple titrimetric assay with soybean lecithin has been used for screening phospholipase D activity from some plant sources, viz. peanut, wheat germ, cabbage and carrot. The enzyme from peanut has been purified by binding to alginate which is a water soluble polymer. The purification consisted of co-precipitation of enzyme with alginate upon addition of 0.06 M Ca++. The enzyme was eluted from the polymer using 0.2 M sodium chloride. The activity recovery was 61% with 34 fold purification.</p>","PeriodicalId":9179,"journal":{"name":"Bioseparation","volume":"9 2","pages":"93-8"},"PeriodicalIF":0.0,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1023/a:1008121005974","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21734412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BioseparationPub Date : 2000-01-01DOI: 10.1023/A:1011165810823
A. V. Sosa, J. Ochoa, N. Perotti
{"title":"Modeling of direct recovery of lactic acid from whole broths by ion exchange adsorption","authors":"A. V. Sosa, J. Ochoa, N. Perotti","doi":"10.1023/A:1011165810823","DOIUrl":"https://doi.org/10.1023/A:1011165810823","url":null,"abstract":"","PeriodicalId":9179,"journal":{"name":"Bioseparation","volume":"193 1","pages":"283-289"},"PeriodicalIF":0.0,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72731823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BioseparationPub Date : 2000-01-01DOI: 10.1023/A:1008120025526
I. Roy, M. N. Gupta
{"title":"Purification of a `double-headed' inhibitor of α-amylase/Proteinase K from wheat germ by expanded bed chromatography","authors":"I. Roy, M. N. Gupta","doi":"10.1023/A:1008120025526","DOIUrl":"https://doi.org/10.1023/A:1008120025526","url":null,"abstract":"","PeriodicalId":9179,"journal":{"name":"Bioseparation","volume":"39 1","pages":"239-245"},"PeriodicalIF":0.0,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85409358","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BioseparationPub Date : 2000-01-01DOI: 10.1023/A:1008132108117
Maria Teresa Cunha, J. Cabral, F. Tjerneld, M. Raquel Aires-Barros
{"title":"Effect of salts and surfactants on the partitioning of Fusarium solani pisi cutinase in aqueous two-phase systems of thermoseparating ethylene oxide/propylene oxide random copolymer and hydroxypropyl starch","authors":"Maria Teresa Cunha, J. Cabral, F. Tjerneld, M. Raquel Aires-Barros","doi":"10.1023/A:1008132108117","DOIUrl":"https://doi.org/10.1023/A:1008132108117","url":null,"abstract":"","PeriodicalId":9179,"journal":{"name":"Bioseparation","volume":"271 1","pages":"203-209"},"PeriodicalIF":0.0,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77574547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BioseparationPub Date : 2000-01-01DOI: 10.1023/A:1011181431742
C. M. Cousins, Jonas Melin, W. Albert Venables, W. Terence Coakley
{"title":"Investigation of enhancement of two processes, sedimentation and conjugation, when bacteria are concentrated in ultrasonic standing waves","authors":"C. M. Cousins, Jonas Melin, W. Albert Venables, W. Terence Coakley","doi":"10.1023/A:1011181431742","DOIUrl":"https://doi.org/10.1023/A:1011181431742","url":null,"abstract":"","PeriodicalId":9179,"journal":{"name":"Bioseparation","volume":"80 1","pages":"343-349"},"PeriodicalIF":0.0,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90502789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BioseparationPub Date : 2000-01-01DOI: 10.1023/A:1011124624001
J. Guil‐Guerrero, E. Belarbi, M. M. Rebolloso-Fuentes
{"title":"Eicosapentaenoic and arachidonic acids purification from the red microalga Porphyridium cruentum","authors":"J. Guil‐Guerrero, E. Belarbi, M. M. Rebolloso-Fuentes","doi":"10.1023/A:1011124624001","DOIUrl":"https://doi.org/10.1023/A:1011124624001","url":null,"abstract":"","PeriodicalId":9179,"journal":{"name":"Bioseparation","volume":"5 1","pages":"299-306"},"PeriodicalIF":0.0,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87062195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}