Bioseparation最新文献

Molecularly imprinted polymer as storage medium for an analyte. 分子印迹聚合物作为分析物的储存介质。

Bioseparation Pub Date : 2001-01-01 DOI: 10.1023/a:1021558207206

K Sreenivasan

引用次数: 4

Study of the factors influencing peak asymmetry on chromatography using a molecularly imprinted polymer prepared by the epitope approach. 表位法制备的分子印迹聚合物对色谱峰不对称影响因素的研究。

Bioseparation Pub Date : 2001-01-01 DOI: 10.1023/a:1021542901754

N Minouraa, A Rachkov, M Higuchi, T Shimizu

{"title":"Study of the factors influencing peak asymmetry on chromatography using a molecularly imprinted polymer prepared by the epitope approach.","authors":"N Minouraa, A Rachkov, M Higuchi, T Shimizu","doi":"10.1023/a:1021542901754","DOIUrl":"https://doi.org/10.1023/a:1021542901754","url":null,"abstract":"Investigations of the effect of sample load on peak asymmetry during chromatography on molecularly imprinted polymer prepared by the epitope approach showed that the shape of the peaks for the template Tyr-Pro-Leu-Gly-NH2 and for acetyl-L-tyrosine ethyl ester changed considerably until a split was observed. In contrast, the asymmetry of the peaks corresponding to oxytocin, which possesses the same C-terminus tripeptide as the template and interacts with the imprinted polymer, remained essentially unaltered. The circular dichroism (CD) spectra of these peptides showed significant dependence on peptide concentration, and the dependence was nearly the same for all the tested peptides. The addition of acetic acid influenced the CD spectra of YPLG and oxytocin but had no influence on the spectrum of acetyl-L-tyrosine ethyl ester. The shape differences in the chromatographic peaks seem to be associated with a solvation mechanism rather than with solute-solute complexation in solution. However, the observed differences in peak asymmetry cannot be completely explained by the mechanisms that have been postulated previously. Our results suggest the formation of triple complexes between a solute molecule (or molecules), an already adsorbed solute molecule, and an adjacent region of the polymeric stationary phase. These triple complexes may influence the retention of analytes and contribute to peak asymmetry.","PeriodicalId":9179,"journal":{"name":"Bioseparation","volume":"10 6","pages":"399-407"},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1023/a:1021542901754","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22215149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

Molecularly imprinted polymeric membranes. 分子印迹聚合物膜。

Bioseparation Pub Date : 2001-01-01 DOI: 10.1023/a:1021537602663

M Yoshikawa

引用次数: 81

Molecular imprinting of surfaces. 表面的分子印迹。

Bioseparation Pub Date : 2001-01-01 DOI: 10.1023/a:1021541631063

I A Nicholls, J P Rosengren

引用次数: 72

Relationship between enantioselectivity of alternative molecularly imprinted polymeric membranes and species of amino acid residues composing chiral recognition sites. 分子印迹聚合物膜对映选择性与组成手性识别位点的氨基酸残基种类的关系。

Bioseparation Pub Date : 2001-01-01 DOI: 10.1023/a:1021593804480

M Yoshikawa, Y Kondo, Y Morita

{"title":"Relationship between enantioselectivity of alternative molecularly imprinted polymeric membranes and species of amino acid residues composing chiral recognition sites.","authors":"M Yoshikawa, Y Kondo, Y Morita","doi":"10.1023/a:1021593804480","DOIUrl":"https://doi.org/10.1023/a:1021593804480","url":null,"abstract":"Molecularly imprinted polymeric membranes with tetrapeptide residue H-Asp(OcHex)-Asp(OcHex)-Asp(OcHex)-Asp(OcHex)-CH2- (DDDD) or H-Glu(OBzl)-Glu(OBzl)-Glu(OBzl)-Glu(OBzl)-CH2- (EEEE) were prepared during membrane preparation (casting) processing in the presence of print molecules. The Boc-L-Trp imprinted polymeric membranes thus obtained showed adsorption selectivity toward Ac-L-Trp from its racemic mixtures. From adsorption isotherms of Ac-Trp, the chiral recognition site, that had been formed by the presence of print molecules in the membrane preparation process, exclusively recognized Ac-L-Trp that possessed the same configuration of the print molecule. The affinity constants between chiral recognition sites in the membrane and Ac-L-Trp was determined to be 1.00 x 10(4) mol(-1) dm3 and 1.08 x 10(4) mol(-1) dm3 for the DDDD and EEEE membranes, respectively. Enantioselective electrodialysis could be attained by applying an optimum potential difference to give permselectivity, with a value close to its adsorption selectivity.","PeriodicalId":9179,"journal":{"name":"Bioseparation","volume":"10 6","pages":"323-30"},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1023/a:1021593804480","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22215206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Selective solid-phase extraction of bio- and environmental samples using molecularly imprinted polymers. 使用分子印迹聚合物的生物和环境样品的选择性固相萃取。

Bioseparation Pub Date : 2001-01-01 DOI: 10.1023/a:1021563931971

L I Andersson

引用次数: 0

Scaled down method for the reproducible recovery to high purity of human serum albumin from low volume blood samples. 从小容量血液样品中可重复回收高纯度人血清白蛋白的缩小方法。

Bioseparation Pub Date : 2001-01-01 DOI: 10.1023/a:1016347102660

M Rynö, T Anttinen-Klemetti, R Vaaranrinta, J Tornaeus, A Hesso, T Veidebaum, K Peltonen

{"title":"Scaled down method for the reproducible recovery to high purity of human serum albumin from low volume blood samples.","authors":"M Rynö, T Anttinen-Klemetti, R Vaaranrinta, J Tornaeus, A Hesso, T Veidebaum, K Peltonen","doi":"10.1023/a:1016347102660","DOIUrl":"https://doi.org/10.1023/a:1016347102660","url":null,"abstract":"A basic need for a protein-based dosimeter is a purified protein. In this communication we present an isolation protocol and an HPLC-based assay which allows one to determine the purity of the isolated albumin. A total of 168 human blood samples were collected from workers of a benzene processing plant and from nearby countryside at Kohtla-Järve, Estonia. Albumin was isolated from plasma by sequential precipitation and the purity was determined by HPLC. The amount of albumin present in plasma varied between the individuals, being 147 +/- 26 mg/5 ml (n = 168), which is about 59% of plasma albumin. However, the isolated albumin was highly pure (100.9 +/- 8.2%, n = 5). All albumin samples analyzed demonstrate two peaks in HPLC analysis. The two peaks detected were collected and subjected to MS analysis, which demonstrates a difference of 120 mass units between the two albumin products isolated. We have developed an assay, which is easy to carry out and is not too labor intense. The HPLC analysis can be applied to confirm the purity of the isolated albumin as well as to confirm the quantity of the albumin in samples.","PeriodicalId":9179,"journal":{"name":"Bioseparation","volume":"10 4-5","pages":"197-201"},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1023/a:1016347102660","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21992533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Capture of proteins from mammalian cells in pilot scale using different STREAMLINE adsorbents 利用不同的流线型吸附剂在中试规模中捕获哺乳动物细胞中的蛋白质

Bioseparation Pub Date : 2001-01-01 DOI: 10.1023/A:1012040708071

D. Lütkemeyer, N. Ameskamp, C. Priesner, Eva-Maria Bartsch, J. Lehmann

引用次数: 6

Study on the scale-up of human IgG3 purification using protein A affinity chromatography. 蛋白A亲和层析法纯化人IgG3的规模化研究。

Bioseparation Pub Date : 2001-01-01 DOI: 10.1023/a:1016353419499

J Amaral, M Inganäs, J Cabral, D Prazeres

{"title":"Study on the scale-up of human IgG3 purification using protein A affinity chromatography.","authors":"J Amaral, M Inganäs, J Cabral, D Prazeres","doi":"10.1023/a:1016353419499","DOIUrl":"https://doi.org/10.1023/a:1016353419499","url":null,"abstract":"The purification of human IgG3 subclass out of IgG (Immunoglobulin-G) was studied using protein A-Sepharose affinity chromatography. The effect of operational parameters such as flow rate, ionic strength, pH and size of sample was investigated, and the process was scaled-up 10-fold. The use of 0.5 m NaCl in the loading buffer had a dramatic effect in the purity of IgG3 recovered in the flowthrough fraction (values in the order of 97% were consistently obtained). This was attributed to a more effective binding of IgG subclasses 1, 2 and 4 to protein A (well known classical mechanism based in Fc fragment) and in some extent to a decrease in the binding of subclass 3 to protein A by the alternative mechanism based in the Fab fragment. The increase in residence time also increased in a relevant way the purity of IgG3. This is attributed to an increased effectiveness of the mechanisms mentioned above. The recovery yields in the IgG3 rich fraction were in the range 21-32% and are possibly a consequence of binding to protein A by the alternative mechanism and also due to deactivation during processing.","PeriodicalId":9179,"journal":{"name":"Bioseparation","volume":"10 4-5","pages":"139-43"},"PeriodicalIF":0.0,"publicationDate":"2001-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1023/a:1016353419499","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21992528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

MIP-ligand binding assays (pseudo-immunoassays). mip配体结合测定(伪免疫测定)。

Bioseparation Pub Date : 2001-01-01 DOI: 10.1023/a:1021502122227

R J Ansell

引用次数: 18