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Fractionation of whey proteins with a hexapeptide ligand affinity resin 用六肽配体亲和树脂分离乳清蛋白
Bioseparation Pub Date : 2000-01-01 DOI: 10.1023/A:1011191818927
P. V. Gurgel, R. Carbonell, H. Swaisgood
{"title":"Fractionation of whey proteins with a hexapeptide ligand affinity resin","authors":"P. V. Gurgel, R. Carbonell, H. Swaisgood","doi":"10.1023/A:1011191818927","DOIUrl":"https://doi.org/10.1023/A:1011191818927","url":null,"abstract":"","PeriodicalId":9179,"journal":{"name":"Bioseparation","volume":"41 1","pages":"385-392"},"PeriodicalIF":0.0,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82668689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 29
Influence of the flocculating agent in sedimentation and performance of a non flocculent strain of Zymomonas mobilis in the ethanol production process. 絮凝剂对乙醇生产过程中沉降及非絮凝性活动单胞菌性能的影响。
Bioseparation Pub Date : 2000-01-01 DOI: 10.1023/a:1008140029482
F P Puget, M R Halasz, G Massarani, T L Alves
{"title":"Influence of the flocculating agent in sedimentation and performance of a non flocculent strain of Zymomonas mobilis in the ethanol production process.","authors":"F P Puget,&nbsp;M R Halasz,&nbsp;G Massarani,&nbsp;T L Alves","doi":"10.1023/a:1008140029482","DOIUrl":"https://doi.org/10.1023/a:1008140029482","url":null,"abstract":"<p><p>The influence of the flocculating agent was studied in the performance (measured by microbial growth and ethanol production) of a non flocculent strain of Zymomonas mobilis, as well as the potentiality of the sedimentation process in the separation of the biomass from the fermentation broth. Among the flocculating agents studied, it was verified that both tannin and the polyelectrolyte yielded good results with regard to cellular performance. However, with regard to sedimentation tannin is more adequate to be used in processes involving Zymomonas mobilis.</p>","PeriodicalId":9179,"journal":{"name":"Bioseparation","volume":"9 3","pages":"119-24"},"PeriodicalIF":0.0,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1023/a:1008140029482","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21929125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 6
Expanded bed chromatography of proteins in small-diameter columns. II. Methods development and scale up. 小直径柱中蛋白质的扩展床层析。2方法开发和推广。
Bioseparation Pub Date : 2000-01-01 DOI: 10.1023/a:1008135528899
S Ghose, H Chase
{"title":"Expanded bed chromatography of proteins in small-diameter columns. II. Methods development and scale up.","authors":"S Ghose,&nbsp;H Chase","doi":"10.1023/a:1008135528899","DOIUrl":"https://doi.org/10.1023/a:1008135528899","url":null,"abstract":"<p><p>The scaled down system developed in Part I of this series was further validated by using a 1-cm diameter column for method development studies for the separation of two model proteins, alcohol dehydrogenase and alpha-glucosidase, from unclarified yeast homogenate by hydrophobic interaction expanded bed chromatography based on the STREAMLINE matrix. The efficacy of solids removal and establishment of optimal binding and separation condition by stepwise elution were investigated. Equilibration of the EBA column and loading at high salt strengths affected the subsequent recovery of the two target proteins. Although good resolution between the target proteins could be achieved, peak tailing was found to be a consistent problem. The optimised separation protocol was scaled up 25-fold to a column diameter of 5.0 cm. The results were in good agreement with the run conducted in the 1-cm column, indicating the potential of using the small columns as an viable approach for method scouting and development studies.</p>","PeriodicalId":9179,"journal":{"name":"Bioseparation","volume":"9 1","pages":"29-36"},"PeriodicalIF":0.0,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1023/a:1008135528899","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21684694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 16
A simple and effective separation and purification procedure for DNA fragments using Dodecyltrimethylammonium bromide 用十二烷基三甲基溴化铵对DNA片段进行简单有效的分离纯化
Bioseparation Pub Date : 2000-01-01 DOI: 10.1023/A:1011182806783
D. McLoughlin, J. O'Brien, J. McManus, A. Gorelov, K. Dawson
{"title":"A simple and effective separation and purification procedure for DNA fragments using Dodecyltrimethylammonium bromide","authors":"D. McLoughlin, J. O'Brien, J. McManus, A. Gorelov, K. Dawson","doi":"10.1023/A:1011182806783","DOIUrl":"https://doi.org/10.1023/A:1011182806783","url":null,"abstract":"","PeriodicalId":9179,"journal":{"name":"Bioseparation","volume":"143 1","pages":"307-313"},"PeriodicalIF":0.0,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75992458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 22
New method for the selective capture of antibodies under physiolgical conditions 在生理条件下选择性捕获抗体的新方法
Bioseparation Pub Date : 2000-01-01 DOI: 10.1023/A:1008170226665
L. Guerrier, P. Girot, W. Schwartz, E. Boschetti
{"title":"New method for the selective capture of antibodies under physiolgical conditions","authors":"L. Guerrier, P. Girot, W. Schwartz, E. Boschetti","doi":"10.1023/A:1008170226665","DOIUrl":"https://doi.org/10.1023/A:1008170226665","url":null,"abstract":"","PeriodicalId":9179,"journal":{"name":"Bioseparation","volume":"45 1","pages":"211-221"},"PeriodicalIF":0.0,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73478337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 92
Contamination of an anion-exchange membrane by glutathione. 阴离子交换膜被谷胱甘肽污染。
Bioseparation Pub Date : 2000-01-01 DOI: 10.1023/a:1008113302507
T Gotoh, K Kikuchi
{"title":"Contamination of an anion-exchange membrane by glutathione.","authors":"T Gotoh,&nbsp;K Kikuchi","doi":"10.1023/a:1008113302507","DOIUrl":"https://doi.org/10.1023/a:1008113302507","url":null,"abstract":"<p><p>Electrodialysis, which can separate electrolytes under mild conditions by using ion-exchange membranes, is a strong candidate for separation of GSH from yeast extracts, because GSH is unstable and easily oxidized forming a disulfide bond especially under alkali conditions. In this paper, sorption behavior of GSH on an anion-exchange membrane, in the pH 3-6 region that is expected to be the most preferable for its electro-dialytic separation, was examined. Sorption of GSH on a Selemion-AMV anion-exchange membrane was accelerated as the pH of the membrane-contact solution increased, and there was a good correlation between the sorbed amounts and the molar fraction of monovalent anionic species of GSH. However, the amounts of GSH desorbed from the membrane by a NaCl desorbing solution were much lower than the initial sorbed amounts, and the difference between them was enlarged with increasing pH. The GSH which was lost could be recovered by the addition of DTT in the membrane-contact and desorbing solutions. Similar results were also obtained with Cys. We thus concluded that an anion-exchange membrane would be contaminated by thiol compounds, such as GSH and Cys, through oxidative binding of the thiol group with the membrane, the local OH- concentration in which was enhanced due to attraction by the positively charged anion-exchange membrane.</p>","PeriodicalId":9179,"journal":{"name":"Bioseparation","volume":"9 1","pages":"37-41"},"PeriodicalIF":0.0,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1023/a:1008113302507","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21684695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 6
Purification and stability of peroxidase of African oil palm Elaies guineensis. 非洲油棕过氧化物酶的纯化及稳定性研究。
Bioseparation Pub Date : 2000-01-01 DOI: 10.1023/a:1008117217885
Sakharov IYu, J Castillo, J C Areza, Galaev IYu
{"title":"Purification and stability of peroxidase of African oil palm Elaies guineensis.","authors":"Sakharov IYu,&nbsp;J Castillo,&nbsp;J C Areza,&nbsp;Galaev IYu","doi":"10.1023/a:1008117217885","DOIUrl":"https://doi.org/10.1023/a:1008117217885","url":null,"abstract":"<p><p>In the previous work, after screening tropical plants (43 species) for peroxidase activity, high activity has been detected in leaves of some palms and especially African oil palm Elaeis guineensis. This palm is widely cultivated in Colombia and presents a promising source for the industrial production of peroxidase. The initial enzyme isolation included homogenization and extraction of pigments using aqueous two phase polymer system. Initially, traditional system, formed by polyethyleneglycol/K2HPO4, was used. The replacement of K2HPO4 with (NH4)2SO4 allowed direct application of the salt phase with accumulated peroxidase on a Phenyl-Sepharose column. The final purification was carried out by liquid chromatography on Sephacryl S200 and DEAE-Toyopearl columns. The specific activity of the purified peroxidase measured toward guaiacol was 4300 units per mg of protein. The molecular weight and isoelectric point for palm peroxidase were 57.000 and 3.8, respectively. Palm peroxidase possesses uniquely high thermostability and is more stable in organic solvents than horseradish peroxidase is.</p>","PeriodicalId":9179,"journal":{"name":"Bioseparation","volume":"9 3","pages":"125-32"},"PeriodicalIF":0.0,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1023/a:1008117217885","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21929126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 61
Purification of alkaline phosphatase from chicken intestine by three-phase partitioning and use of phenyl-Sepharose 6B in the batch mode. 采用三相分割法纯化鸡肠碱性磷酸酶并分批使用苯基- sepharose 6B。
Bioseparation Pub Date : 2000-01-01 DOI: 10.1023/a:1008195729472
A Sharma, S Sharma, M N Gupta
{"title":"Purification of alkaline phosphatase from chicken intestine by three-phase partitioning and use of phenyl-Sepharose 6B in the batch mode.","authors":"A Sharma,&nbsp;S Sharma,&nbsp;M N Gupta","doi":"10.1023/a:1008195729472","DOIUrl":"https://doi.org/10.1023/a:1008195729472","url":null,"abstract":"<p><p>Alkaline phosphatase from chicken intestine was purified from the crude preparation employing three-phase partitioning and by the use of phenyl Sepharose-6B in the batch mode. TPP uses a combination of ammonium sulphate and t-butanol to precipitate proteins from crude aqueous extracts. The precipitated protein forms interface between lower aqueous phase and upper organic solvent phase. The fold purification of the finally purified enzyme was 80 and the activity recovery was 61%. The sodium dodecyl sulphate-polyacrylamide gel electrophoresis analysis of enzyme showed considerable purification and its molecular weight was found to be around 67 kDa.</p>","PeriodicalId":9179,"journal":{"name":"Bioseparation","volume":"9 3","pages":"155-61"},"PeriodicalIF":0.0,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1023/a:1008195729472","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21929129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 31
Analytical and Preparative Separation Methods of Biomacromolecules Hassan Y. Aboul-Enein (Ed.) 生物大分子的分析和制备分离方法
Bioseparation Pub Date : 2000-01-01 DOI: 10.1023/A:1008151009004
J. Kennedy, M. Thorley
{"title":"Analytical and Preparative Separation Methods of Biomacromolecules Hassan Y. Aboul-Enein (Ed.)","authors":"J. Kennedy, M. Thorley","doi":"10.1023/A:1008151009004","DOIUrl":"https://doi.org/10.1023/A:1008151009004","url":null,"abstract":"","PeriodicalId":9179,"journal":{"name":"Bioseparation","volume":"46 1","pages":"57"},"PeriodicalIF":0.0,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86716084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Comparison of different types of ceramic hydroxyapatite for the chromatographic separation of plasmid DNA and a recombinant anti-Rhesus D antibody 比较不同类型的陶瓷羟基磷灰石用于质粒DNA和重组抗恒河猴D抗体的色谱分离
Bioseparation Pub Date : 2000-01-01 DOI: 10.1023/A:1011174905511
R. Giovannini, R. Freitag
{"title":"Comparison of different types of ceramic hydroxyapatite for the chromatographic separation of plasmid DNA and a recombinant anti-Rhesus D antibody","authors":"R. Giovannini, R. Freitag","doi":"10.1023/A:1011174905511","DOIUrl":"https://doi.org/10.1023/A:1011174905511","url":null,"abstract":"","PeriodicalId":9179,"journal":{"name":"Bioseparation","volume":"30 1","pages":"359-368"},"PeriodicalIF":0.0,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90986430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 34
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