Biorheology最新文献_第5页

Effect of arterial curvature on hemodynamics and mass transport. 动脉曲度对血流动力学和物质运输的影响。

IF 1.1 4区医学

Biorheology Pub Date : 2019-01-01 DOI: 10.3233/BIR-190215

Sen Lu, Shusheng Zhang

{"title":"Effect of arterial curvature on hemodynamics and mass transport.","authors":"Sen Lu, Shusheng Zhang","doi":"10.3233/BIR-190215","DOIUrl":"https://doi.org/10.3233/BIR-190215","url":null,"abstract":"Background: Atherosclerotic lesions develop preferentially at certain sites in the human arterial system, such as the inner wall of curved segments and the outer wall of bifurcations. Local wall shear stress (WSS) and concentration of low density lipoprotein (LDL) have been identified as two important factors contributing to these lesions.Objective: To determine if a connection exists between arterial curvature and the formation of atherosclerosis.Methods: A set of 3-D vessel models with different bend angles was constructed. By comparing blood flow, WSS, and LDL aggregation, the influence of bend curvature on atherosclerotic lesions was assessed.Results: Upon increasing arterial bending, low WSS regions were formed at the outer wall of the junction between straight and curved segments, as well as the inner wall of curved segments. However, high LDL concentrations only appeared at the inner wall of the bend region. A connection between secondary flow and LDL concentration was observed; high LDL concentration regions had stronger secondary flow. Higher water infiltration velocity could enhance LDL aggregation, while blood non-Newtonian properties, by easing secondary flow, diminished its aggregation.Conclusions: Under the same flow rate, a larger bend angle increased flow resistance, lowered WSS, and increased LDL surface concentrations, thus indicating an increased risk of atherosclerosis.","PeriodicalId":9167,"journal":{"name":"Biorheology","volume":"56 4","pages":"253-263"},"PeriodicalIF":1.1,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3233/BIR-190215","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37125896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Nitrite may serve as a combination partner and a biomarker for the anti-cancer activity of RRx-001. 亚硝酸盐可以作为RRx-001抗癌活性的联合伙伴和生物标志物。

IF 1.1 4区医学

Biorheology Pub Date : 2019-01-01 DOI: 10.3233/bir-190213

S. Cırrık, E. Ugurel, A. C. Aksu, B. Oronsky, P. Cabrales, O. Yalcin

{"title":"Nitrite may serve as a combination partner and a biomarker for the anti-cancer activity of RRx-001.","authors":"S. Cırrık, E. Ugurel, A. C. Aksu, B. Oronsky, P. Cabrales, O. Yalcin","doi":"10.3233/bir-190213","DOIUrl":"https://doi.org/10.3233/bir-190213","url":null,"abstract":"BACKGROUND RRx-001 is an anti-cancer immunotherapeutic that increases the sensitivity of drug resistant tumors via multiple mechanisms which involve binding to hemoglobin and enhancing nitrite reductase activity of deoxyhemoglobin. OBJECTIVE In the present study, the effect of clinically used doses of RRx-001 on erythrocyte deformability was examined. METHODS A dose dependent effect of RRx-001 (1-1000 micro molar) on erythrocyte deformability was measured by ektacytometer under hypoxia (n = 8). Low dose RRx-001 (20 micro molar) in the presence of ODQ (1H-[1,2,4]Oxadiazolo[4,3-a]quinoxalin-1-one), L-NAME (L-NG-Nitroarginine methyl ester) or nitrite were examined both in normoxia and hypoxia. Intracellular nitric oxide (NO) levels were measured fluorometrically with DAF-FM-DA. RESULTS Higher doses of RRx-001 (100, 1000 micro molar) significantly decreased erythrocyte deformability under hypoxia (p < 0.01; p < 0.05, respectively). RRx-001 (20 micro molar), alone or in combination with ODQ or L-NAME, did not change deformability. However, RRx-001 and nitrite caused an increase in deformability (p < 0.01) under hypoxia. RRx-001 induced NO production was more pronounced in the presence of nitrite (p < 0.05). CONCLUSIONS Co-administration of RRx-001 and nitrite under hypoxic conditions results in a significant increase in erythrocyte deformability that is related to increased NO production. We suggest that measurement of serum nitrite level in RRx-001 treated cancer patients should be routinely undertaken and supplemented if levels are low for maximal activity.","PeriodicalId":9167,"journal":{"name":"Biorheology","volume":"1 1","pages":""},"PeriodicalIF":1.1,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3233/bir-190213","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69793460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Endothelial barrier reinforcement relies on flow-regulated glycocalyx, a potential therapeutic target. 内皮屏障的强化依赖于血流调节的糖萼，这是一个潜在的治疗靶点。

IF 1.1 4区医学

Biorheology Pub Date : 2019-01-01 DOI: 10.3233/BIR-180205

Ian C Harding, Ronodeep Mitra, Solomon A Mensah, Alina Nersesyan, Nandita N Bal, Eno E Ebong

{"title":"Endothelial barrier reinforcement relies on flow-regulated glycocalyx, a potential therapeutic target.","authors":"Ian C Harding, Ronodeep Mitra, Solomon A Mensah, Alina Nersesyan, Nandita N Bal, Eno E Ebong","doi":"10.3233/BIR-180205","DOIUrl":"https://doi.org/10.3233/BIR-180205","url":null,"abstract":"Background: The onset of many disease processes depends on the function of the endothelial cell (EC) glycocalyx (GCX) which acts as a flow-dependent barrier to cellular infiltration and molecular transport across the blood vessel wall.Objective: This review aims to examine these processes with the potential end goal of implementing GCX repair to restore EC barrier function and slow the progression of disease.Methods: Cell and mouse studies were employed to examine the state of EC GCX in healthy versus disruptive flow conditions. Correlations of observations of the GCX with a number of EC functions were sought with an emphasis on studies of trans-endothelial barrier integrity against vessel wall infiltration of cells and molecules from the circulation. To demonstrate the importance of GCX as a regulator of trans-endothelial infiltration, assays were performed using ECs with an intact GCX and compared to assays of ECs with an experimentally degraded GCX. Studies were also conducted of ECs in which a degraded GCX was repaired.Results: In healthy flow conditions, the EC GCX was found to be thick and substantially covered the endothelial surface. GCX expression dropped significantly in complex flow conditions and coincided with a disease-like cellular and molecular accumulation in the endothelium or within the blood vessel wall. Therapeutic repair of the GCX abolished this accumulation.Conclusions: Regenerating the degraded GCX reverses EC barrier dysfunction and may attenuate the progression of vascular disease.","PeriodicalId":9167,"journal":{"name":"Biorheology","volume":" ","pages":"131-149"},"PeriodicalIF":1.1,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3233/BIR-180205","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37154150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 18

Blood flow distribution and the endothelial surface layer in the diabetic retina. 糖尿病视网膜的血流分布和内皮表面层。

IF 1.1 4区医学

Biorheology Pub Date : 2019-01-01 DOI: 10.3233/BIR-180200

Norman R Harris, Wendy Leskova, Gaganpreet Kaur, Randa S Eshaq, Patsy R Carter

引用次数: 7

Understanding endothelial glycocalyx function under flow shear stress from a molecular perspective. 从分子角度了解血流剪切应力作用下内皮糖萼的功能。

IF 1.1 4区医学

Biorheology Pub Date : 2019-01-01 DOI: 10.3233/BIR-180193

Xi Zhuo Jiang, Yufang Lu, Kai H Luo, Yiannis Ventikos

{"title":"Understanding endothelial glycocalyx function under flow shear stress from a molecular perspective.","authors":"Xi Zhuo Jiang, Yufang Lu, Kai H Luo, Yiannis Ventikos","doi":"10.3233/BIR-180193","DOIUrl":"https://doi.org/10.3233/BIR-180193","url":null,"abstract":"Background: The endothelial glycocalyx plays a pivotal role in regulating blood flow, filtering blood components, sensing and transducing mechanical signals. These functions are intimately related to its dynamics at the molecular level.Objective: The objective of this research is to establish the relationship between the functions of the endothelial glycocalyx and its dynamics at the molecular level.Methods: To establish such a relationship, large-scale molecular dynamics simulations were undertaken to mimic the dynamics of the glycocalyx and its components in the presence of flow shear stresses.Results: First, motions of the glycocalyx core protein and the pertinent subdomains were scrutinised. Three-directional movements of the glycocalyx core protein were observed, although the flow was imposed only in the x direction. Such an observation contributes to understanding the glycocalyx redistribution as reported in experiments. Unsynchronised motion of the core protein subdomains was also spotted, which provides an alternative explanation of macroscopic phenomena. Moreover, the dynamics, root-mean-square-deviations and conformational changes of the sugar chains were investigated. Based on the findings, an alternative force transmission pathway, the role of sugar chains, and potential influence on signalling transduction pathways were proposed and discussed.Conclusions: This study relates the functions of the glycocalyx with its microscopic dynamics, which fills a knowledge gap about the links between different scales.","PeriodicalId":9167,"journal":{"name":"Biorheology","volume":" ","pages":"89-100"},"PeriodicalIF":1.1,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3233/BIR-180193","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37043023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Endothelial glycocalyx restoration by growth factors in diabetic nephropathy. 生长因子在糖尿病肾病中的内皮糖萼修复作用。

IF 1 4区医学

Biorheology Pub Date : 2019-01-01 DOI: 10.3233/BIR-180199

Sara Desideri, Karen L Onions, Siân L Baker, Monica Gamez, Hesham El Hegni E Hussien, Amy Russell, Simon C Satchell, Rebecca R Foster

{"title":"Endothelial glycocalyx restoration by growth factors in diabetic nephropathy.","authors":"Sara Desideri, Karen L Onions, Siân L Baker, Monica Gamez, Hesham El Hegni E Hussien, Amy Russell, Simon C Satchell, Rebecca R Foster","doi":"10.3233/BIR-180199","DOIUrl":"10.3233/BIR-180199","url":null,"abstract":"The endothelial glycocalyx (eGlx) constitutes the first barrier to protein in all blood vessels. This is particularly noteworthy in the renal glomerulus, an ultrafiltration barrier. Leakage of protein, such as albumin, across glomerular capillaries results in albumin in the urine (albuminuria). This is a hall mark of kidney disease and can reflect loss of blood vessel integrity in microvascular beds elsewhere. We discuss evidence demonstrating that targeted damage to the glomerular eGlx results in increased glomerular albumin permeability. EGlx is lost in diabetes and experimental models demonstrate loss from glomerular endothelial cells. Vascular endothelial growth factor (VEGF)A is upregulated in early diabetes, which is associated with albuminuria. Treatment with paracrine growth factors such as VEGFC, VEGF165b and angiopoietin-1 can modify VEGFA signalling, rescue albumin permeability and restore glomerular eGlx in models of diabetes. Manipulation of VEGF receptor 2 signalling, or a common eGlx biosynthesis pathway by these growth factors, may protect and restore the eGlx layer. This would help to direct future therapeutics in diabetic nephropathy.","PeriodicalId":9167,"journal":{"name":"Biorheology","volume":" ","pages":"163-179"},"PeriodicalIF":1.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37295423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Numerical investigation of the effects of blood rheology and wall elasticity in abdominal aortic aneurysm under pulsatile flow conditions. 脉动血流条件下腹主动脉瘤血液流变学和壁弹性影响的数值研究。

IF 1.1 4区医学

Biorheology Pub Date : 2019-01-01 DOI: 10.3233/BIR-180202

Coşkun Bilgi, Kunt Atalık

{"title":"Numerical investigation of the effects of blood rheology and wall elasticity in abdominal aortic aneurysm under pulsatile flow conditions.","authors":"Coşkun Bilgi, Kunt Atalık","doi":"10.3233/BIR-180202","DOIUrl":"https://doi.org/10.3233/BIR-180202","url":null,"abstract":"Background: Previous studies on aneurysm modeling have focused on the blood rheology and vessel elasticity separately. The combined effects of blood shear thinning properties and wall elasticity need to be revealed.Objective: To provide insights on how pulsatile hemodynamics vary with blood rheology and vessel elasticity for a developed abdominal aortic aneurysm (AAA).Method: An Arbitrary Lagrangian-Eulerian fluid-solid interaction method is adopted with the Newtonian and the shear thinning Carreau constitutive models for the fluid with the linearly elastic and the hyperelastic Yeoh models for the vessel. Finite element based numerical solver is used to simulate the blood flow in the AAA.Results: Newtonian model overestimates the velocity values compared to the Carreau model and the difference in the velocity field increases as the shear rate decreases at the instances of the cardiac cycle. The rigid walled simulations display higher deviations in the velocity and wall shear stress with the fluid rheology. The risk indicators show that Newtonian assumption combined with the linearly elastic model may overlook degeneration risk of arterial tissue.Conclusions: Newtonian assumption for the blood as well as modelling the arterial wall as linearly elastic lead to significant differences in oscillatory hemodynamic properties with respect to the use of Carreau fluid together with hyperelastic vessel model, even in large vessel aneurysms.","PeriodicalId":9167,"journal":{"name":"Biorheology","volume":"56 1","pages":"51-71"},"PeriodicalIF":1.1,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3233/BIR-180202","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37366758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Comparative adhesive and migratory properties of mesenchymal stem cells from different tissues. 不同组织间充质干细胞粘附和迁移特性的比较。

IF 1.1 4区医学

Biorheology Pub Date : 2019-01-01 DOI: 10.3233/BIR-180185

Asma Alanazi, Hafsa Munir, Mohammed Alassiri, Lewis S C Ward, Helen M McGettrick, Gerard B Nash

{"title":"Comparative adhesive and migratory properties of mesenchymal stem cells from different tissues.","authors":"Asma Alanazi, Hafsa Munir, Mohammed Alassiri, Lewis S C Ward, Helen M McGettrick, Gerard B Nash","doi":"10.3233/BIR-180185","DOIUrl":"https://doi.org/10.3233/BIR-180185","url":null,"abstract":"Background: Mesenchymal stem cells (MSC) are used in therapy, often by injection into the blood.Objective: We aimed to compare the adhesive and migratory properties of MSC from umbilical cords (UCMSC), bone marrow (BMMSC) or trabecular bone (TBMSC), which might influence delivery to injured tissue.Methods: MSC were perfused through glass capillaries coated with matrix proteins, collagen or fibronectin, or albumin. Adherent cells were counted microscopically and their spreading analysed over time. MSC migration through 8 μm pore filters coated with the same proteins was analysed.Results: The number of MSC adhering to collagen was greater than fibronectin, decreased as wall shear rate increased from 17 to 70 s-1, and was in the order UCMSC>BMMSC>TBMSC. Conversely, spreading was more effective on fibronectin and was in the order BMMSC>TBMSC≥UCMSC. Migration was promoted by coating the lower surface of filters with either matrix protein, with UCMSC migrating more efficiently than BMMSC.Conclusions: MSC show origin-dependent variations in their efficiency of capture from flow and subsequent spreading or ability to migrate on matrix proteins. UCMSC showed most efficient capture from flow, which was followed by less spreading, but more rapid migration. These responses might be associated with more effective delivery from the circulation into damaged tissue.","PeriodicalId":9167,"journal":{"name":"Biorheology","volume":"56 1","pages":"15-30"},"PeriodicalIF":1.1,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3233/BIR-180185","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36926421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

Tensile behavior and extensional viscosity of bile. 胆汁的拉伸性能和拉伸粘度。

IF 1.1 4区医学

Biorheology Pub Date : 2019-01-01 DOI: 10.3233/bir-190216

N. Minh, H. Obara, K. Shimokasa, Junfang Zhu

{"title":"Tensile behavior and extensional viscosity of bile.","authors":"N. Minh, H. Obara, K. Shimokasa, Junfang Zhu","doi":"10.3233/bir-190216","DOIUrl":"https://doi.org/10.3233/bir-190216","url":null,"abstract":"BACKGROUND Bile, which is secreted by the liver, is essential for digesting fat and maintaining homeostasis. Although the rheology of bile is very important to its flow, its extensional viscosity has not been studied. OBJECTIVE We investigated the stretching of bile and evaluated its extensional viscosity using a filament-breakup device. METHODS A liquid bridge formed between the endplates of the filament-breakup device. The midpoint diameter of the bridge was recorded by a laser micrometer. The filament self-thinning was captured by a high-resolution, high-speed camera. We used the liquid bridge diameter to evaluate the extensional viscosity of bile. RESULTS All samples show shear-thinning and viscoelasticity. Bile sediment has much greater viscosity and stretches more than bile solution. Filament thinning takes a long time for bile sediment and a short time for solution. The global function for the mid-filament diameter can be used to predict the evolution of the filament diameter of the liquid bridge and the trend of the extensional viscosity of bile. CONCLUSIONS The extensional viscosity of bile is much greater than its shear viscosity. Because mucus concentration makes bile sediment more viscous than the solution, bile viscosity can increase sharply, thereby increasing risks of such diseases as gallstones and sludge buildup in the biliary system.","PeriodicalId":9167,"journal":{"name":"Biorheology","volume":"1 1","pages":""},"PeriodicalIF":1.1,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3233/bir-190216","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69793300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Correlation of the cell mechanical behavior and quantified cytoskeletal parameters in normal and cancerous breast cell lines. 正常和癌乳腺细胞系中细胞力学行为和定量细胞骨架参数的相关性。

IF 1.1 4区医学

Biorheology Pub Date : 2019-01-01 DOI: 10.3233/bir-190214

M. Tabatabaei, M. Tafazzoli-Shadpour, M. Khani

{"title":"Correlation of the cell mechanical behavior and quantified cytoskeletal parameters in normal and cancerous breast cell lines.","authors":"M. Tabatabaei, M. Tafazzoli-Shadpour, M. Khani","doi":"10.3233/bir-190214","DOIUrl":"https://doi.org/10.3233/bir-190214","url":null,"abstract":"BACKGROUND Cancerous transformation of cells affects their mechanical behavior and cytoskeleton structure. OBJECTIVE The objective of this research is to investigate a correlation between mechanical properties and cytoskeletal structure features in cancer cell formation. METHODS Micropipette aspiration was used to compare mechanical properties of normal (MCF10A) and cancerous (T47D) epithelial breast cell lines. Immunofluorescence and confocal microscopy were employed for staining and imaging F-actin and microtubules, and quantifying their fluorescent intensity, anisotropy and fiber distribution. RESULTS Results indicated higher F-actin intensity (43%) and anisotropy (50%) in normal cells compared to cancer cells, although there was no difference in the microtubules intensity between cell lines. Furthermore, reductions of cortex thickness and actin layer index (60%) were observed in suspended cancer cells compared to normal cells. Changes in cell physical properties induced by cancer were attributed to microtubules. The arranged fibrous structure of microtubules in normal cells was replaced by a disorganized structure in cancer cells. Cancerous cells were about four times softer with higher creep compliance compared to normal cells. CONCLUSIONS Results of this study confirmed that alterations in cell mechanical properties induced by cancer are highly correlated with changes in F-actin and microtubule content and arrangement. It is suggested that such changes can enhance our knowledge of cancer initiation and progression.","PeriodicalId":9167,"journal":{"name":"Biorheology","volume":"1 1","pages":""},"PeriodicalIF":1.1,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3233/bir-190214","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69793631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10