Virology & mycology : infectious diseases最新文献_第4页

Corona Virus ORF1ab-Derived Nsp9 and Nsp10Non-Structural Proteins have Homologies to S8/S10 Ribosomal Proteins as well as RlmG/ ErmDrRNAMethyltransferasesand may Inhibit Host Mitoribosome Assembly and Protein Synthesis 冠状病毒orf1ab衍生的Nsp9和nsp10非结构蛋白与S8/S10核糖体蛋白以及RlmG/ ermdrrname甲基转移酶具有同源性，并可能抑制宿主线粒体组装和蛋白质合成

Virology & mycology : infectious diseases Pub Date : 2020-01-01 DOI: 10.35248/2161-0517.20.09.186

A. Chakraborty

{"title":"Corona Virus ORF1ab-Derived Nsp9 and Nsp10Non-Structural Proteins have Homologies to S8/S10 Ribosomal Proteins as well as RlmG/ ErmDrRNAMethyltransferasesand may Inhibit Host Mitoribosome Assembly and Protein Synthesis","authors":"A. Chakraborty","doi":"10.35248/2161-0517.20.09.186","DOIUrl":"https://doi.org/10.35248/2161-0517.20.09.186","url":null,"abstract":"Multi-Alignment method coupled with phylogenetic analysis we disclosed the Nsp9 and Nsp10 non-structural proteins of Corona Virus asrRNARlmH/K methyltransferases with similarities with bin recombinase and int-core integrase fold. Further, Nsp9 has similarities to S8 ribosomal protein and Nap10 has similarity to S10 ribosomal protein. Previously, we showed Nsp13, Nsp14, Nsp15 and Nsp16 are also different types of rRNARlmE/N and Cfr-like methyltransferases-ribonucleasewith RNA helicase domains. Two domains of Nsp13 astonishingly have similarities to ribosomal proteins L6 and L9. Taken together, Nsp9/10 and Nsp13-16 proteins could mimic host ribosome assembly and also could methylate rRNA of mitobibosome preventing mitochondrial protein synthesis and oxidative phosphorylation. Low ATP synthesis causes lowering blood pressure following coma but very ATP concentration (1-10nM) surely induces platelets aggregation through vWA, collagen and GpIIb/IIIaproteins followed byfibrin formation and blood clotting as recently have seen in the lung of many Corona virus infected patients. We have also postulated that two polyproteins itself resemble like 28S and 38S mitoribosome subunits and compete with rRNAs inhibiting the ribosome turnover and new protein synthesis due to their similarities with many ribosomal proteins.Such finding may be valuable in computer-based novel drug design against Corona virus.","PeriodicalId":91631,"journal":{"name":"Virology & mycology : infectious diseases","volume":"9 1","pages":"1-6"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69977440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Virology & Mycology Overview 病毒学与真菌学综述

Virology & mycology : infectious diseases Pub Date : 2020-01-01 DOI: 10.35248/2161-0517.20.09.E112

Xiaobao Ying

引用次数: 0

MALDI-TOF Clinical Filamentous Fungi Identification. Bead Beating Versus Acid Solvent Protein Extraction Method MALDI-TOF临床丝状真菌鉴定。头部加热与酸溶剂蛋白质萃取法

Virology & mycology : infectious diseases Pub Date : 2020-01-01 DOI: 10.29011/2688-8750.100019

G. Corvino, M. Rocca, V. Folliero, F. Foglia, G. Franci, E. Finamore, M. Galdiero

引用次数: 0

SARS-CoV-2 and about N95 Covering Mask SARS-CoV-2和N95覆盖口罩

Virology & mycology : infectious diseases Pub Date : 2020-01-01 DOI: 10.35248/2161-0517.20.09.E113

Xiaobao Ying

引用次数: 0

Occurrence of the Slippery Sequence UUUAAAC in the RNA Genome 2 that generates the ORF1ab Protein of SARS-CoV-2 产生SARS-CoV-2 ORF1ab蛋白的RNA基因组2中光滑序列UUUAAAC的出现

Virology & mycology : infectious diseases Pub Date : 2020-01-01 DOI: 10.35248/2161-0517.20.9.198

H. Geurdes

引用次数: 0

Virology & mycology : infectious diseases Pub Date : 2018-09-03 DOI: 10.4172/2155-6113-C1-026

pYetnayet Abebe Melaku Haile Likka Tolossa Wakayo, Mulusew Gerbabap

{"title":"Health related quality of life and associated factors among women on anti-retroviral therapy in health facilities of Jimma Town, Southwest Ethiopia: A cross-sectional study","authors":"pYetnayet Abebe Melaku Haile Likka Tolossa Wakayo, Mulusew Gerbabap","doi":"10.4172/2155-6113-C1-026","DOIUrl":"https://doi.org/10.4172/2155-6113-C1-026","url":null,"abstract":"Background: Dengue is a mosquito-borne viral disease that has rapidly spread in all regions in recent years. There is little information on dengue fever epidemiology in Iran. High prevalence of dengue fever in Pakistan bordering southeast Iran emphasizes the need for paying more attention to monitoring of the disease in this region. The aim of this study was to study the dengue fever seropositivity among adult febrile patients in southeast Iran. \u0000Methods:Dengue fever virus specific IgG antibodies were measured by a commercial enzyme-linked immunosorbent assay (ELISA) in sera of 184 patients. \u0000Results: All examined sera were negative. \u0000Conclusions:The existence of dengue fever in eastern borders of Iran and tracking the virus vectors in the southeast Iran can be a treat for the circulation of the virus in Iran; however, we did not find any track of the seropositivity in this study and despite the high prevalence of dengue fever in Pakistan bordering southeast Iran, no dengue positive cases were found in this study. This epidemiological research is significant for local health authorities.","PeriodicalId":91631,"journal":{"name":"Virology & mycology : infectious diseases","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70278410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 13

Human herpesvirus type 8 in patients with chronic hepatitis 人疱疹病毒8型在慢性肝炎患者中的作用

Virology & mycology : infectious diseases Pub Date : 2018-08-04 DOI: 10.4172/2161-0517-C3-029

ChengChuan Su

引用次数: 0

Bioinformatic approaches to infectious diseases 传染病的生物信息学方法

Virology & mycology : infectious diseases Pub Date : 2018-07-12 DOI: 10.4172/2161-0517-C2-025

T. Jiang

{"title":"Bioinformatic approaches to infectious diseases","authors":"T. Jiang","doi":"10.4172/2161-0517-C2-025","DOIUrl":"https://doi.org/10.4172/2161-0517-C2-025","url":null,"abstract":"T development of safe, oncolytic (o) HSV vectors for systemic treatment of metastatic breast cancer provides an opportunity for combining virus lytic activity with the potential to induce anti-tumor immunity. Ideally oHSV should be engineered for selective infection of tumor cells and escape from neutralizing antibodies. Complete retargeting strategies both detarget the viral attachment/ entry glycoprotein gD from recognition of its cognate receptors, HVEM and nectin-1, and provide gD with a novel ligand that recognizes a highly expressed tumor-associated receptors that are poorly or not expressed on non-tumor tissue. Here we present design strategies for engineered oHSV that preferentially infect and lyse breast cancer cells by recognition of GFRα1, a receptor highly expressed on a subset of estrogen receptor-positive breast cancers. We replaced the signal peptide and HVEM binding domain of gD with pre-pro-(pp)GDNF to create a GFRα1 targeting protein, gD(Y38)-GDNF, that can still bind nectin-1. Virus expressing gD(Y38)-GDNF was propagated on cells expressing nectin-1 and purified virus was shown to enter nectin-1/HVEM-deficient J1.12 and B78H1 cells in a GFRα1-dependent manner. U2OS cells engineered to express GFRα1 were found to support propagation of a fully retargeted derivative virus that no longer recognizes nectin-1 but selectively infects cells through recognition of GFRα1, resulting in MOI-dependent tumor cell death in vitro. Moreover, direct intratumoral injection in nude mice showed complete tumor destruction in vivo. We have discovered, however, that retargeting can reduce the efficiency of virus entry and increase sensitivity to neutralizing (VN) antibodies, both associated with reduced retargeted gD incorporation into the virus envelope. Efficient infectivity was partially restored by selective residue changes in the downstream fusogen gB. Current studies involve engineered and selected changes in the epitope structure of retargeted gD that allow escape from VN antibodies to permit efficient systemic application of oHSV for metastatic breast cancer therapy.","PeriodicalId":91631,"journal":{"name":"Virology & mycology : infectious diseases","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70463000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immuno-profiling of non-tuberculous mycobacterial infection patients reveal global T cell dysfunction and individuals at risk 非结核分枝杆菌感染患者的免疫分析揭示了整体T细胞功能障碍和个体的风险

Virology & mycology : infectious diseases Pub Date : 2018-05-28 DOI: 10.4172/2161-0517-C1-023

Scott C Bell

引用次数: 1

CRISPR, A New Powerful Weapon for Plant to Combat Viruses CRISPR，植物对抗病毒的强大新武器

Virology & mycology : infectious diseases Pub Date : 2018-02-07 DOI: 10.4172/2161-0517.1000E111

Xiaobao Ying

引用次数: 2