Virology & mycology : infectious diseases最新文献_第10页

Influenza severe cases and deaths in Tunisia: Season 2015-2016 突尼斯的流感严重病例和死亡:2015-2016年季节

Virology & mycology : infectious diseases Pub Date : 2016-01-01 DOI: 10.4172/2161-0517.C1.015

H. Bouguerra

引用次数: 1

MERS Outbreak: Is India Prepared? 中东呼吸综合征爆发:印度准备好了吗?

Virology & mycology : infectious diseases Pub Date : 2015-12-07 DOI: 10.4172/2161-0517.1000E110

C. N. Kumar, P. Reddy, K. Ramalingam

引用次数: 2

Competitive Enzyme Linked Immuno-Sorbent Assay (c-ELISA) Based Sero-Prevalence of Bluetongue Virus (BTV) on Small Ruminants in SelectedAreas of Wolyita, Southern Ethiopia 基于竞争酶联免疫吸附试验(c-ELISA)的埃塞俄比亚南部Wolyita地区小反刍动物蓝舌病病毒(BTV)血清流行率研究

Virology & mycology : infectious diseases Pub Date : 2015-12-07 DOI: 10.4172/2161-0517.1000148

Melese Yilma, M. Mekonnen

引用次数: 13

Compatibility Studies of Fungicides with Combination of TrichodermaSpecies under In vitro Conditions 杀真菌剂与木霉菌种组合的体外相容性研究

Virology & mycology : infectious diseases Pub Date : 2015-12-07 DOI: 10.4172/2161-0517.1000149

Bikila Wedajo

引用次数: 14

Quantitative Detection of Antibodies to Aleutian Disease Virus in DriedBlood Spots as an Estimation of Hypergammaglobulinemia in Mink 水貂干血斑阿留申病病毒抗体定量检测评价高γ球蛋白血症

Virology & mycology : infectious diseases Pub Date : 2015-11-12 DOI: 10.4172/2161-0517.1000147

A. Andersson, H. B. Reineck, A. Nyman, P. Wallgren

{"title":"Quantitative Detection of Antibodies to Aleutian Disease Virus in DriedBlood Spots as an Estimation of Hypergammaglobulinemia in Mink","authors":"A. Andersson, H. B. Reineck, A. Nyman, P. Wallgren","doi":"10.4172/2161-0517.1000147","DOIUrl":"https://doi.org/10.4172/2161-0517.1000147","url":null,"abstract":"Infections with Aleutian disease virus (ADV) cause a progressive hypergammaglobulinemia, immune complex formation and plasma cell infiltrations in internal organs which induce a multi-systemic disease with high mortality. Serum ADV antibodies have traditionally been diagnosed with counter immunoelectrophoresis (CIEP) as a gold standard for qualitative assessment separating infected from non-infected animals, but less laborious ELISA methods have been confirmed to be equally sensitive. A way to simplify the diagnostics further could be to demonstrate antibodies in Dried blood spot samples (DBS). However, quantitative analysis of ADV antibodies in DBS and its correlation to the degree of hypergammaglobulinemia have not been scientifically published. The aim of this paper was to describe the adaptation and validation of the VP2 ELISA to ADV antibody detection in DBS and compare the estimated antibody levels in DBS to CIEP results, the estimated antibody levels and albumin: gamma globulin ratio in serum. The VP2 ELISA worked technically well when transferred from serum to DBS with mean intra-assay and mean inter-assay coefficients of variation within ± 20%. The DBS VP2 ELISA had a sensitivity of 97.3% and specificity of 93.2% compared to CIEP. Further, we found a correlation coefficient between the level of antibodies in DBS and the A:γG ratio of -0.81. The correlation between the A:γG ratio and the OD450 value was superior in DBS compared to serum samples from the same mink with the most pronounced difference at low A:γG ratios. Our results confirmed that the VP2 ELISA could detect ADV antibodies in DBS with a high sensitivity and specificity when employing CIEP as gold standard. The antibody titers estimated with DBS VP2 ELISA were well correlated to the antibody titters and A:γG ratios in serum, and the DBS VP2 ELISA could be an applicable and preferable tool for estimating AD progression in mink.","PeriodicalId":91631,"journal":{"name":"Virology & mycology : infectious diseases","volume":"5 1","pages":"0-0"},"PeriodicalIF":0.0,"publicationDate":"2015-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4172/2161-0517.1000147","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70456001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Infection Control is One Major Key to Longevity 控制感染是长寿的关键之一

Virology & mycology : infectious diseases Pub Date : 2015-10-23 DOI: 10.4172/2161-0517.1000E109

G. Kotwal, S. Chien

引用次数: 2

Evaluation of Commonly Used Rapid Test Kits and Serological Serial TestingAlgorithm for Diagnosis of HIV-1 and 2 Antibodies in Port Harcourt, Nigeria 尼日利亚哈科特港HIV-1和2抗体诊断常用快速检测试剂盒和血清学系列检测算法的评价

Virology & mycology : infectious diseases Pub Date : 2015-09-29 DOI: 10.4172/2161-0517.1000146

Matthew Osaro, Frank-Peterside, Okonko Io, E. Ughala, Obike-Martins

{"title":"Evaluation of Commonly Used Rapid Test Kits and Serological Serial TestingAlgorithm for Diagnosis of HIV-1 and 2 Antibodies in Port Harcourt, Nigeria","authors":"Matthew Osaro, Frank-Peterside, Okonko Io, E. Ughala, Obike-Martins","doi":"10.4172/2161-0517.1000146","DOIUrl":"https://doi.org/10.4172/2161-0517.1000146","url":null,"abstract":"Three diagnostic rapid test kits (Chembio HIV-1/2 Stat-Pak, Alere DetermineTM HIV-1/2, and Core HIV-1/2) for human immunodeficiency virus types 1 and 2 (HIV-1/2) antibodies detection were evaluated against a gold standard fourth generation ELISA (Dia-Pro). Hundred and seventy two serum samples were collected from Lulubriggs Health Center, University of Port Harcourt, Rivers State. All samples were tested for HIV-1/2 using the three rapid test (parallel algorithm and serial algorithm), and retested using the 4th generation ELISA. The results obtained were compared with those from the ELISA. The DetermineTM rapid strips had sensitivity of 66.7%, specificity of 100%, positive predictive value 100%, negative predictive value 89.4% and total agreement of 91.3%. Stat-pak and Core HIV-1/2 rapid strips both had sensitivity of 71.7%, specificity of 100%, positive predictive value 100%, and negative predictive value 90.7% and total agreement of 92.4%. Determine was mostly used by the participant laboratories 18 of 30 (60%). The performance evaluation of the testing algorithm showed that parallel algorithm is more accurate in comparison to serial algorithm. The result of this study reveals that most rapid test are less sensitive and the accuracy of serial testing algorithm is low, this implies that most acute HIV infection will be missed with rapid assay and false negative results will be reported as actual negative with serial algorithm.","PeriodicalId":91631,"journal":{"name":"Virology & mycology : infectious diseases","volume":"4 1","pages":"0-0"},"PeriodicalIF":0.0,"publicationDate":"2015-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70455799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Development and Evaluation of an Antigen Capture Enzyme-LinkedImmunosorbent Assay (AC-ELISA) for the Diagnosis of African Swine fever 抗原捕获酶联免疫吸附试验(AC-ELISA)诊断非洲猪瘟的建立与评价

Virology & mycology : infectious diseases Pub Date : 2015-08-10 DOI: 10.4172/2161-0517.1000145

Jamaluddin Majid

{"title":"Development and Evaluation of an Antigen Capture Enzyme-LinkedImmunosorbent Assay (AC-ELISA) for the Diagnosis of African Swine fever","authors":"Jamaluddin Majid","doi":"10.4172/2161-0517.1000145","DOIUrl":"https://doi.org/10.4172/2161-0517.1000145","url":null,"abstract":"African swine fever (ASF) is a viral haemorrhagic fever of pigs with devastating impact on pig production and household income in Africa. Lack of a vaccine and treatment for ASF has increased the dependence on accurate diagnosis as basis for control and possible eradication of the disease. The aim of this study therefore, was to develop and evaluate an in-house sandwich enzyme linked immune sorbent assay (ELISA) using antibodies raised against African swine fever virus (ASFv) isolates from Uganda for diagnosis of ASF. The ASFv was grown in pig alveolar macrophages, the infected cells harvested, lysed and the virus precipitated using polyethylene glycol 6000. The virus was purified on Sepadex G-200 column equilibrated with 50mM Tris-HCl PH 7.2 containing 0.15M NaCl and viral proteins separated by SDS-PAGE. The target protein (vp73), was quantified and used to immunize rabbits to produce polyclonal antibodies against it. The purified immunoglobulin IgG (rabbit anti ASF-vp73) was used for antigen capture in sandwich ELISA. Eighty eight (88) known positive and 176 known negative pig serum samples were used to evaluate assay performance. The diagnostic sensitivity of the ELISA was 82.95% (95% CI, 78-100%), diagnostic specificity was 96.59% (95% CI, 90–100%). Positive and negative predictive values were 92.4% and 91%, respectively. The inter samples coefficient of variation of raw optical density values for known positive samples in different runs was <10% (range 1.1-7.8), while intra sample coefficient of variation ranged from 0.6-5.5% between runs. The developed antigen capture ELISA has a high diagnostic sensitivity and specificity and is therefore good for detection of active ASF infection. However, the developed assay should be further validated using larger sample size under different laboratory conditions and referenced serum samples from different ASF endemic countries.","PeriodicalId":91631,"journal":{"name":"Virology & mycology : infectious diseases","volume":"4 1","pages":"1-8"},"PeriodicalIF":0.0,"publicationDate":"2015-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4172/2161-0517.1000145","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70455718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

influenza vaccine produced in E. coli elicits protective HAI titers in healthy adults 大肠杆菌生产的流感疫苗在健康成人中引起保护性的HAI滴度

Virology & mycology : infectious diseases Pub Date : 2015-07-24 DOI: 10.4172/2161-0517.S1.004

Scott Umlauf

引用次数: 0

The role of mHealth in the fight against flu 移动医疗在抗击流感中的作用

Virology & mycology : infectious diseases Pub Date : 2015-07-24 DOI: 10.4172/2161-0517.S1.005

Bipin Thomas

{"title":"The role of mHealth in the fight against flu","authors":"Bipin Thomas","doi":"10.4172/2161-0517.S1.005","DOIUrl":"https://doi.org/10.4172/2161-0517.S1.005","url":null,"abstract":"A of vaccines by novel needle-free technology such as jet injection offers an important alternative to needle and syringe and may enhance compliance. In August 2014, needle-free vaccination against influenza was approved with Afluria using PharmaJet® Stratis® jet injection technology. The objective of this post-marketing study was to assess acceptance of and satisfaction with flu vaccination using Afluria delivered in the grocery pharmacy setting via the novel needle-free system. A total of 98 grocery store customers, ages 18-64 were administered needle-free Afluria vaccination during the 20142015 flu season using the PharmaJet device and agreed to complete a short post-administration survey. The population of was 54% female and 74% of all respondents reported receiving a flu shot the prior season. Overall, 89% of subjects reported being satisfied, very satisfied or extremely satisfied with the needle-free flu shot; 83% of subjects reported that they were likely, very likely or definitely going to choose flu vaccination by jet injection next year. The high degree of satisfaction with Afluria delivered through needle-free PharmaJet Stratis suggests that needle-free vaccination with jet injection may be widely accepted, improving compliance in the general adult population which currently has very low rates of immunization against flu.","PeriodicalId":91631,"journal":{"name":"Virology & mycology : infectious diseases","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2015-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70461226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2