{"title":"基于竞争酶联免疫吸附试验(c-ELISA)的埃塞俄比亚南部Wolyita地区小反刍动物蓝舌病病毒(BTV)血清流行率研究","authors":"Melese Yilma, M. Mekonnen","doi":"10.4172/2161-0517.1000148","DOIUrl":null,"url":null,"abstract":"The objective of the present study is to assess the sero-prevalence and associated risk factors for small ruminants’ bluetongue infection in selected agro-ecology of woliyta zone, southern Ethiopia. Serum samples were collected randomly from the accessed small ruminates and screened for detection of BTV-specific immunoglobulin G (IgG) antibodies using a competitive enzyme-linked immunosorbent assay (c-ELISA). A total of 476 serum samples were tested and 196 (41.17%) were positive for bluetongue virus antibodies. A prevalence rate ranging from 26.53% for the midland altitude to 73.47% for the lowland was recorded. To disease associated risk factor age, and location was recorded using multivariate analysis of logistic regression model. Within species goat 114 (58.2%) and sheep 82 (41.8%) sero positive reactors was recorded. This is the first report indicating the presence of bluetongue virus infection in the area.","PeriodicalId":91631,"journal":{"name":"Virology & mycology : infectious diseases","volume":"4 1","pages":"1-4"},"PeriodicalIF":0.0000,"publicationDate":"2015-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"13","resultStr":"{\"title\":\"Competitive Enzyme Linked Immuno-Sorbent Assay (c-ELISA) Based Sero-Prevalence of Bluetongue Virus (BTV) on Small Ruminants in SelectedAreas of Wolyita, Southern Ethiopia\",\"authors\":\"Melese Yilma, M. Mekonnen\",\"doi\":\"10.4172/2161-0517.1000148\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The objective of the present study is to assess the sero-prevalence and associated risk factors for small ruminants’ bluetongue infection in selected agro-ecology of woliyta zone, southern Ethiopia. Serum samples were collected randomly from the accessed small ruminates and screened for detection of BTV-specific immunoglobulin G (IgG) antibodies using a competitive enzyme-linked immunosorbent assay (c-ELISA). A total of 476 serum samples were tested and 196 (41.17%) were positive for bluetongue virus antibodies. A prevalence rate ranging from 26.53% for the midland altitude to 73.47% for the lowland was recorded. To disease associated risk factor age, and location was recorded using multivariate analysis of logistic regression model. Within species goat 114 (58.2%) and sheep 82 (41.8%) sero positive reactors was recorded. This is the first report indicating the presence of bluetongue virus infection in the area.\",\"PeriodicalId\":91631,\"journal\":{\"name\":\"Virology & mycology : infectious diseases\",\"volume\":\"4 1\",\"pages\":\"1-4\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2015-12-07\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"13\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Virology & mycology : infectious diseases\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.4172/2161-0517.1000148\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Virology & mycology : infectious diseases","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.4172/2161-0517.1000148","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Competitive Enzyme Linked Immuno-Sorbent Assay (c-ELISA) Based Sero-Prevalence of Bluetongue Virus (BTV) on Small Ruminants in SelectedAreas of Wolyita, Southern Ethiopia
The objective of the present study is to assess the sero-prevalence and associated risk factors for small ruminants’ bluetongue infection in selected agro-ecology of woliyta zone, southern Ethiopia. Serum samples were collected randomly from the accessed small ruminates and screened for detection of BTV-specific immunoglobulin G (IgG) antibodies using a competitive enzyme-linked immunosorbent assay (c-ELISA). A total of 476 serum samples were tested and 196 (41.17%) were positive for bluetongue virus antibodies. A prevalence rate ranging from 26.53% for the midland altitude to 73.47% for the lowland was recorded. To disease associated risk factor age, and location was recorded using multivariate analysis of logistic regression model. Within species goat 114 (58.2%) and sheep 82 (41.8%) sero positive reactors was recorded. This is the first report indicating the presence of bluetongue virus infection in the area.