Postdoc journal : a journal of postdoctoral research and postdoctoral affairs最新文献_第3页

Rot1, an essential yeast protein, is degraded through the ER-associated protein degradation system (ERAD) Rot1是一种重要的酵母蛋白，通过内质网相关蛋白降解系统（ERAD）降解

Postdoc journal : a journal of postdoctoral research and postdoctoral affairs Pub Date : 2017-11-01 DOI: 10.14304/SURYA.JPR.V5N11.2

M. Juanes, C. Martínez-Garay, M. Bañó

{"title":"Rot1, an essential yeast protein, is degraded through the ER-associated protein degradation system (ERAD)","authors":"M. Juanes, C. Martínez-Garay, M. Bañó","doi":"10.14304/SURYA.JPR.V5N11.2","DOIUrl":"https://doi.org/10.14304/SURYA.JPR.V5N11.2","url":null,"abstract":"S. cerevisiae ROT1 is an essential gene which has been related to cell wall biosynthesis, the actin cytoskeleton and protein folding. Rot1 protein is primarily located at the endoplasmic reticulumnuclear membrane facing the lumen where it is translocated through two internal topogenic elements by an SRP-independent posttranslational mechanism which depends on Sec62 to then be N-glycosylated at the lumen of the ER. Despite Rot1 protein levels are critical for yeast survival, proper cell cycle progression and morphogenesis, Rot1 protein levels has not been investigated so far. Here we carry out an in vivo study to analyse the Rot1 protein levels and show that Rot1 is a short-lived protein and its turnover is mediated by the ubiquitin proteasome system (UPS), dependent on the main degradation pathway located at the ER, the ER-associated degradation system (ERAD). This finding correlates with genetic interactions we previously found and that suggested a link between Rot1 and the ubiquitin-proteasome system. In addition, by using mutant cells components of the ERAD pathway, we demonstrated that Rot1 is degraded through the ubiquitin conjugating enzymes (E2) components of ERAD, Ubc6 and Ubc7, and it seems to require only one of the E3 ubiquitin ligases involved in ERAD, Hrd1 but not Doa1.","PeriodicalId":90946,"journal":{"name":"Postdoc journal : a journal of postdoctoral research and postdoctoral affairs","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41774987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Targeting DNA repair as cancer therapy: TLS comes into focus 靶向DNA修复作为癌症治疗:TLS成为焦点

Postdoc journal : a journal of postdoctoral research and postdoctoral affairs Pub Date : 2017-07-20 DOI: 10.14304/SURYA.JPR.V5N9.1

Jia Zhou

引用次数: 0

Understanding the Potential Barriers and Facilitators for Enrolling Postdoctoral Fellows: A Case Study of Medical Colleges in Malawi, Zambia, and Zimbabwe 了解招收博士后的潜在障碍和促进因素:马拉维、赞比亚和津巴布韦医学院的案例研究

Postdoc journal : a journal of postdoctoral research and postdoctoral affairs Pub Date : 2017-07-20 DOI: 10.14304/SURYA.JPR.V5N9.3

W. Mandala, Amelia Challender, M. Maimbolwa, E. Gomo, M. Nyirenda, F. Cowan, Susan C. Connors

{"title":"Understanding the Potential Barriers and Facilitators for Enrolling Postdoctoral Fellows: A Case Study of Medical Colleges in Malawi, Zambia, and Zimbabwe","authors":"W. Mandala, Amelia Challender, M. Maimbolwa, E. Gomo, M. Nyirenda, F. Cowan, Susan C. Connors","doi":"10.14304/SURYA.JPR.V5N9.3","DOIUrl":"https://doi.org/10.14304/SURYA.JPR.V5N9.3","url":null,"abstract":"In many countries, aspiring researchers compete for post-doctoral fellowships after PhD studies. The Southern Africa Consortium of Research Excellence (SACORE) had aimed to enrol eight post-doctoral fellows in the first phase but only appointed one. This study was conducted to explore barriers and potential facilitators for enrolment of post-doctoral fellows at three African schools of medicine. Individual interviews were conducted with 32 stakeholders from College of Medicine, Malawi; University of Zambia, School of Medicine; and University of Zimbabwe, College of Health Sciences. Interviewees included: university leaders and faculty (n = 15), Ministry leaders (n = 5), eligible fellowship candidates (n = 11), and a post-doctoral fellow (n = 1). Interview guides were developed for each stakeholder group; interviews were recorded, transcribed verbatim and coded for key themes. Post-doctoral fellowships were identified as potentially beneficial financially and for academic career development. Institutions felt they could benefit by developing relevant research, increasing the pool of mentors, and future research leaders. Lack of suitably qualified applicants and economic constraints of academia were the main challenges to recruitment. Post-doctoral fellowships benefits for individuals, institutions and countries, and the need to improve the recruitment process as well as to define research career pathways, were isolated.","PeriodicalId":90946,"journal":{"name":"Postdoc journal : a journal of postdoctoral research and postdoctoral affairs","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46027988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cyanobacterial biofactories: combining evolved and synthetic genetic regulatory mechanisms to yield carbon-neutral bioproducts 蓝藻生物工厂:结合进化和合成的遗传调控机制，产生碳中性生物产品

Postdoc journal : a journal of postdoctoral research and postdoctoral affairs Pub Date : 2017-07-20 DOI: 10.14304/SURYA.JPR.V5N7.4

L. A. Brand, G. Owttrim

引用次数: 0

Natural Killer Cells in Cancer: An Overview 癌症中的自然杀伤细胞：综述

Postdoc journal : a journal of postdoctoral research and postdoctoral affairs Pub Date : 2017-07-20 DOI: 10.14304/SURYA.JPR.V5N9.4

A. Rao, N. Amankulor

引用次数: 0

EDITORIAL: Metal Hydrides as Catalytic Intermediates 编辑：金属氢化物作为催化中间体

Postdoc journal : a journal of postdoctoral research and postdoctoral affairs Pub Date : 2017-07-20 DOI: 10.14304/SURYA.JPR.V5N7.1

Sarah R Soltau

引用次数: 0

Metal Hydrides as Catalytic Intermediates: The Curious Case of Iron Carbonyl Clusters 金属氢化物作为催化中间体：羰基铁簇合物的奇特案例

Postdoc journal : a journal of postdoctoral research and postdoctoral affairs Pub Date : 2017-07-20 DOI: 10.14304/SURYA.JPR.V5N7.2

Atefeh Taheri, Louise A. Berben

引用次数: 0

Translesion Synthesis: An emerging new target during chemotherapy 翻译合成:化疗中的新靶点

Postdoc journal : a journal of postdoctoral research and postdoctoral affairs Pub Date : 2017-07-20 DOI: 10.14304/SURYA.JPR.V5N9.2

C. Harris, Nimrat Chatterjee

{"title":"Translesion Synthesis: An emerging new target during chemotherapy","authors":"C. Harris, Nimrat Chatterjee","doi":"10.14304/SURYA.JPR.V5N9.2","DOIUrl":"https://doi.org/10.14304/SURYA.JPR.V5N9.2","url":null,"abstract":"Translesion synthesis (TLS) is an important mechanism where a group of polymerases come together to orchestrate the bypass of aberrant DNA lesions. This process enables survival of those cells, which would have otherwise been committed to die because of these difficult to repair DNA lesions. Interestingly, siRNA knockdown of TLS polymerases in cancer cells was shown to sensitize cells to increased killing and reduced mutagenesis following treatment with chemotherapeutic drugs, which suggests an important role of TLS polymerases in chemoresistance and cancer relapse. Recent investigations showed that small molecule inhibitors (SMi) that specifically bind REV1 TLS polymerases and suppressed its protein-protein interactions with other TLS polymerases, also successfully sensitized cancer cells to enhanced killing and reduced mutagenesis. As such, the possibility of a SMi that specifically binds REV1 and inhibits TLS, will serve as a chemotherapy adjuvant and prevent chemoresistance and cancer relapse is now a promising possibility. In this mini-review, we will provide a brief discourse of the overarching importance of TLS polymerases in normal and cancer cells and describe characteristics of current and future TLS inhibitors that will potentially serve as clinical chemotherapeutic adjuvants.","PeriodicalId":90946,"journal":{"name":"Postdoc journal : a journal of postdoctoral research and postdoctoral affairs","volume":"20 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67014799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Role of the Spindle and kinetochore associated (Ska) protein complex in mitotic progression 纺锤体和动粒相关（Ska）蛋白复合体在有丝分裂过程中的作用

Postdoc journal : a journal of postdoctoral research and postdoctoral affairs Pub Date : 2017-07-20 DOI: 10.14304/SURYA.JPR.V5N9.6

Sushama Sivakumar

{"title":"Role of the Spindle and kinetochore associated (Ska) protein complex in mitotic progression","authors":"Sushama Sivakumar","doi":"10.14304/SURYA.JPR.V5N9.6","DOIUrl":"https://doi.org/10.14304/SURYA.JPR.V5N9.6","url":null,"abstract":"The Spindle and Kinetochore Associated (Ska) protein complex is a heterotrimeric complex involved in mitotic progression. Depletion of Ska1, Ska2 or Ska3 by RNAi causes delays in chromosome alignment followed by a long metaphase arrest that results in Cohesion fatigue. Ska1 and Ska3 have been shown to bind microtubules both in vitro and in vivo. Ska1 directly recruits the protein phosphatase PP1 to kinetochores during mitosis. The Ska complex has also been implicated is regulating the localization of Anaphase promoting complex/cyclosome (APC/C) to chromosomes. Aurora B kinase has been shown to phosphorylate and regulate kinetochore recruitment of Ska proteins. The Ska complex, in addition to being substrate of Aurora B, also directly activates Aurora B and regulates kinetochore microtubule stability to ensure proper chromosome bi-orientation. The master mitotic kinase Cdk1 phosphorylates Ska3 and regulates Ska complex localization to kinetochores. Cdk1 kinase does not affect spindle localization of the Ska complex suggesting that distinct pathways exist to recruit Ska complex to defined mitotic substructures. Herein we discuss these findings and postulate their implications on Ska complex function during mitosis.","PeriodicalId":90946,"journal":{"name":"Postdoc journal : a journal of postdoctoral research and postdoctoral affairs","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47900242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Research Highlight: Revealing molecular mechanism behind the effect of Zika Virus infection on neurodevelopment 研究重点:揭示寨卡病毒感染对神经发育影响的分子机制

Postdoc journal : a journal of postdoctoral research and postdoctoral affairs Pub Date : 2017-07-20 DOI: 10.14304/SURYA.JPR.V5N7.5

Parikshit Bagchi

引用次数: 0