Biotechnology & Biotechnological Equipment最新文献_第10页

Comparative study on the metabolic behaviour of anode biofilm in microbial fuel cell under different external resistance 不同外阻条件下微生物燃料电池阳极生物膜代谢行为的比较研究

IF 1.4 4区生物学

Biotechnology & Biotechnological Equipment Pub Date : 2022-12-31 DOI: 10.1080/13102818.2022.2055491

Yana Mersinkova, Hyusein Yemendzhiev, V. Nenov

引用次数: 1

Two QTL for kernel number per spike identified from durum wheat 硬粒小麦每穗粒数的两个QTL

IF 1.4 4区生物学

Biotechnology & Biotechnological Equipment Pub Date : 2022-12-31 DOI: 10.1080/13102818.2022.2054728

Jingtian Yang, Zhengqiao Liao, Baoguo Du, K. Zhang, Lei Liu

引用次数: 0

An immobilization-based, loop-mediated isothermal amplification device for nucleic acid detection of SARS-CoV-2 N gene 基于固定化环介导的sars - cov - 2n基因核酸检测等温扩增装置

IF 1.4 4区生物学

Biotechnology & Biotechnological Equipment Pub Date : 2022-12-31 DOI: 10.1080/13102818.2022.2134822

Tianyu Song, Chang Chen, Shaoheng Bao, Bin Du, Xiaokun Wang, Jiajia Liu, Fuli Wang, Wei Ma, G. Yao, Xiukun Wan, Xinlong Zhang, JingJing Wang, Hui Jiang

{"title":"An immobilization-based, loop-mediated isothermal amplification device for nucleic acid detection of SARS-CoV-2 N gene","authors":"Tianyu Song, Chang Chen, Shaoheng Bao, Bin Du, Xiaokun Wang, Jiajia Liu, Fuli Wang, Wei Ma, G. Yao, Xiukun Wan, Xinlong Zhang, JingJing Wang, Hui Jiang","doi":"10.1080/13102818.2022.2134822","DOIUrl":"https://doi.org/10.1080/13102818.2022.2134822","url":null,"abstract":"Abstract Confronting the global spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), simple, fast and specific non-laboratory SARS-CoV-2 diagnostic tests are urgently required. However, the current nucleic acid assays generally rely on the diagnostic laboratory, trained staff and specialized equipment for execution and analysis, presenting clear limitations in the field detection. Here, we describe a portable and reliable immobilization-based loop-mediated isothermal amplification (LAMP) device which is mobile, without the requirement of any complicated instrument and appropriate for high-throughput testing. This device was constructed by utilizing the interaction between a carboxyl-tagged primer and an amino-tagged substrate, and capable of catching the target sequence in SARS-CoV-2 produced via the immobilization-based LAMP. In this study, the immobilization conditions and immobilized primer structure were explored and optimized. With this proposed device, the analysis result can be obtained rapidly in 30 min with excellent specificity, even if the template is extracted from a complex sample containing pharyngeal swab or human blood. In addition, the device can be applied to detect the nucleic acid of SARS-CoV-2 and various other pathogens, showing attractive potential for rapid and high-throughput detection at airports, railway stations, cold-chain transportations, community hospitals and so on. Therefore, we believe that the immobilization-based LAMP device is an advanced approach to developing a portable, specific, low-cost and high-throughput diagnostic platform.","PeriodicalId":9076,"journal":{"name":"Biotechnology & Biotechnological Equipment","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47635864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Autologous ovarian platelet rich plasma treatment improves oocyte and embryo quality: a before-after prospective study 自体卵巢富血小板血浆治疗改善卵母细胞和胚胎质量:一项前后前瞻性研究

IF 1.4 4区生物学

Biotechnology & Biotechnological Equipment Pub Date : 2022-12-31 DOI: 10.1080/13102818.2022.2090280

D. Parvanov, R. Ganeva, N. Vidolova, K. Nikolova, Magdalena Vasileva, T. Totev, G. Stamenov

{"title":"Autologous ovarian platelet rich plasma treatment improves oocyte and embryo quality: a before-after prospective study","authors":"D. Parvanov, R. Ganeva, N. Vidolova, K. Nikolova, Magdalena Vasileva, T. Totev, G. Stamenov","doi":"10.1080/13102818.2022.2090280","DOIUrl":"https://doi.org/10.1080/13102818.2022.2090280","url":null,"abstract":"Abstract Platelet-rich plasma (PRP) is used for successful regeneration of female reproductive tissues. However, little is known about the effect of ovarian PRP treatment on oocyte and embryo quality. The objective of our study was to assess the role of autologous ovarian PRP treatment on ovarian reserve and number and quality of oocytes and embryos in women with poor ovarian response (POR) undergoing in-vitro fertilization cycles. A total of 66 women with POR were treated with ovarian PRP injection in two subsequent menstrual cycles. The antral follicle counts, serum anti-Mullerian hormone, follicle-stimulating hormone levels, fertilization rate, number and quality of oocytes, and embryos were assessed and compared between the cycle before and after PRP treatment. Ovarian PRP treatment resulted in insignificantly lower follicle-stimulating hormone levels, significantly higher antral follicle count, anti-Mullerian hormone, number of retrieved oocytes, and insignificantly higher fertilization rate. However, the mean number of Day 5 embryos (2.19 ± 1.45 vs. 1.58 ± 1.30, p = 0.01), the percentage of high-quality oocytes (45.29% ± 42.40% vs. 15.21% ± 30.24%, p < 0.01) and the percentage of grade-I blastocysts (52.10% ± 37.94% vs. 12.86% ± 22.97%, p < 0.01) were significantly higher after PRP treatment in comparison to the pretreatment period. Moreover, the mean MII oocyte quality (1.60 ± 0.54 vs. 2.31 ± 0.63, p < 0.01) and mean blastocyst quality (1.53 ± 0.45 vs. 2.42 ± 0.63, p < 0.01) were significantly improved in the post-treatment period. In conclusion, the applied autologous ovarian PRP treatment in poor responders may significantly improve oocyte and embryo quality.","PeriodicalId":9076,"journal":{"name":"Biotechnology & Biotechnological Equipment","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42153847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Optimization of succinic acid production from xylose mother liquor (XML) by Actinobacillus succinogenes using response surface methodology 响应面法优化琥珀酸放线菌木糖母液产琥珀酸的工艺条件

IF 1.4 4区生物学

Biotechnology & Biotechnological Equipment Pub Date : 2022-12-31 DOI: 10.1080/13102818.2022.2095303

N. Shen, Shiyong Li, Yan Qin, Mingguo Jiang, Hongyan Zhang

{"title":"Optimization of succinic acid production from xylose mother liquor (XML) by Actinobacillus succinogenes using response surface methodology","authors":"N. Shen, Shiyong Li, Yan Qin, Mingguo Jiang, Hongyan Zhang","doi":"10.1080/13102818.2022.2095303","DOIUrl":"https://doi.org/10.1080/13102818.2022.2095303","url":null,"abstract":"Abstract In the present study, conditions for succinic acid (SA) production using xylose mother liquor (XML) as culture medium by Actinobacillus succinogenes GXAS137 were optimized. Firstly, single–factor experiments were performed to evaluate the basal culture medium for SA fermentation. Thereafter, the Plackett–Burman design was used to screen out three significant factors of XML, corn steep liquor powder (CSLP) and MgCO3 affecting the SA yields from the original nine factors. Subsequent use of steepest ascent experiment determined the center area of the three factors. Finally, the response surface methodology was used to further optimize the interactions between the three main factors and predict the maximum SA concentration through Box-Behnken design. The optimal conditions of SA fermentation were maximally documented in the XML (110 g/L), CSLP (18.86 g/L) and MgCO3 (69.12 g/L). The maximum production of SA was 58.06 ± 0.57 g/L after 60 h with a yield of 0.72 ± 0.06 g/g total sugar, approaching the predicted value (57.99 g/L). It was 1.63-fold of the SA production obtained with the basic medium (35.54 g/L). In addition, batch fermentations were carried out in a 1.3-L stirred bioreactor and SA reached 58.47 g/L. These results indicate that XML could be an alternative substrate for the economical production of SA by A. succinogenes GXAS137.","PeriodicalId":9076,"journal":{"name":"Biotechnology & Biotechnological Equipment","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48203924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Enhancing precision medicine through clinical mass spectrometry platform 通过临床质谱平台提升精准医疗

IF 1.4 4区生物学

Biotechnology & Biotechnological Equipment Pub Date : 2022-12-31 DOI: 10.1080/13102818.2022.2053342

J. Hristova, D. Svinarov

{"title":"Enhancing precision medicine through clinical mass spectrometry platform","authors":"J. Hristova, D. Svinarov","doi":"10.1080/13102818.2022.2053342","DOIUrl":"https://doi.org/10.1080/13102818.2022.2053342","url":null,"abstract":"Abstract There is an extraordinary flood of new technologies in medicine nowadays. Sophisticated diagnostics based on genome assays, mass spectrometry and cell sorting platforms are driving the technological transfer and promote the entrance of individualized patient management in clinical practice. Mass spectrometry (MS) could be viewed as one of the major tools that promote the development of precision medicine (PM), which employs a patient’s genotype and phenotype investigation to establish individually tailored drug treatment. While genetic testing allows the physician to choose appropriate medicine, MS assays provide the patient’s actual phenotype, with all of the environmental, pharmacological and pathological variables. Therefore, MS is an essential technology for personalized patient management, and high-resolution MS systems are employed to resolve challenging analytical demands. The great technological advance of MS resulted in the introduction of methods with unprecedented identification power, extreme sensitivity, specificity and extended linearity range, which are simpler to use in the medical laboratories, and are based on the current reference analytical principles. Further, the ability to perform panel profiling with simultaneous measurement of bioactive compounds, their precursors and metabolites in a single sample, enormously amplifies the informative value of results, with ultimate improvement of patient care. Typical examples include newborn screening, therapeutic drug management, toxicology, endocrinology, microbiology, clinical omics assays and others. It should be specially emphasized that clinical MS integrates chemical and anatomical pathology: MS imaging and iKnife-MS guidance in surgery, although still in the research phase, open new horizons for personalized treatment and individualized patient care.","PeriodicalId":9076,"journal":{"name":"Biotechnology & Biotechnological Equipment","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44336164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inhibitory activity of carbon quantum dots against Phytophthora infestans and fungal plant pathogens and their effect on dsRNA-induced gene silencing 碳量子点对晚疫病和真菌植物病原体的抑制活性及其对dsRNA诱导的基因沉默的影响

IF 1.4 4区生物学

Biotechnology & Biotechnological Equipment Pub Date : 2022-11-21 DOI: 10.1080/13102818.2022.2146533

K. Kostov, Boika Andonova-Lilova, G. Smagghe

{"title":"Inhibitory activity of carbon quantum dots against Phytophthora infestans and fungal plant pathogens and their effect on dsRNA-induced gene silencing","authors":"K. Kostov, Boika Andonova-Lilova, G. Smagghe","doi":"10.1080/13102818.2022.2146533","DOIUrl":"https://doi.org/10.1080/13102818.2022.2146533","url":null,"abstract":"Abstract Carbon quantum dots (CQDs) have many potential applications due to their cell-penetrating ability, biocompatibility and tunable properties. Among a variety of characteristics, the inhibition of bacteria by CQDs is often reported. However, the effect on other microorganisms, such as plant pathogenic fungi and oomycetes, is poorly studied. Here we monitored the growth of the oomycete plant pathogen Phytophthora infestans in the presence of CQDs, as well as of another three fungal plant pathogens, namely Botrytis cinerea, Alternaria alternata and Fusarium oxysporum. Moreover, the ability of CQDs to improve gene silencing caused by exogenous dsRNA in P. infestans was studied, and the toxicity of CQDs to human keratinocytes was evaluated. Our results indicate significant inhibitory activity of CQDs against P. infestans at relatively low concentrations. In a species-specific manner and to a lesser extent, the growth of the three fungal plant pathogens was also affected. We also found that the treatment of P. infestans with naked dsRNA in vitro did not trigger gene silencing. However, the mixture of CQDs with dsRNA increased RNAi efficiency, by causing a significant reduction of the transcript levels of the target gene in developing sporangia. Finally, no cytotoxicity of the CQDs, in the concentrations active against the plant pathogens, was found.","PeriodicalId":9076,"journal":{"name":"Biotechnology & Biotechnological Equipment","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2022-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41309125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

High rate of fluoroquinolone resistant Neisseria gonorrhoeae detected by molecular surveillance of antimicrobial resistance determinants in Bulgaria 保加利亚抗微生物耐药性决定因素的分子监测检测到耐氟喹诺酮类药物的淋病奈瑟菌的高比率

IF 1.4 4区生物学

Biotechnology & Biotechnological Equipment Pub Date : 2022-11-11 DOI: 10.1080/13102818.2022.2146532

I. Philipova, V. Levterova, I. Simeonovski, T. Kantardjiev

{"title":"High rate of fluoroquinolone resistant Neisseria gonorrhoeae detected by molecular surveillance of antimicrobial resistance determinants in Bulgaria","authors":"I. Philipova, V. Levterova, I. Simeonovski, T. Kantardjiev","doi":"10.1080/13102818.2022.2146532","DOIUrl":"https://doi.org/10.1080/13102818.2022.2146532","url":null,"abstract":"Abstract Antimicrobial-resistant Neisseria gonorrhoeae is a major public health concern. The surveillance of antimicrobial resistance benefits from rapid and accurate molecular techniques in molecular diagnostics to facilitate individualised medicine and antimicrobial stewardship. To support the recommendations for empirical treatment of gonococcal infections in Bulgaria, we investigated N. gonorrhoeae-positive clinical specimens from 2018 to 2021 for the presence of genetic determinants associated with antimicrobial resistance. N. gonorrhoeae-positive samples stored at the National Center of Infectious and Parasitic Diseases during the four-year study period were retrospectively analysed by polymerase chain reaction and DNA sequencing assays for resistance determinants to fluoroquinolones, third-generation cephalosporins and macrolides. The detected determinants indicated a high rate of fluoroquinolone resistance (59%), very low level of decreased susceptibility to third-generation cephalosporins (3%) but no macrolide resistance (0%). These findings validate the utilisation of the international guidelines’ recommendations for empirical dual therapy with ceftriaxone/cefixime and azithromycin in Bulgaria. Because of the high fluoroquinolone resistance rate, ciprofloxacin should only be considered as treatment if phenotypic or molecular antimicrobial susceptibility data indicate susceptibility to ciprofloxacin. For the purposes of surveillance and individualised medicine, molecular assays for resistance determinants could complement culture-based phenotypic gonococcal antimicrobial resistance testing.","PeriodicalId":9076,"journal":{"name":"Biotechnology & Biotechnological Equipment","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2022-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48397554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Light filtration technology for sustainable microalgal biomass production 可持续微藻生物量生产的光过滤技术

IF 1.4 4区生物学

Biotechnology & Biotechnological Equipment Pub Date : 2022-11-07 DOI: 10.1080/13102818.2022.2144455

E. T. Sero, N. Siziba, Tavengwa Bunhu, R. Shoko

{"title":"Light filtration technology for sustainable microalgal biomass production","authors":"E. T. Sero, N. Siziba, Tavengwa Bunhu, R. Shoko","doi":"10.1080/13102818.2022.2144455","DOIUrl":"https://doi.org/10.1080/13102818.2022.2144455","url":null,"abstract":"Abstract The use of native microalgal strains, which are well adapted to local environmental conditions, for sustainable biofuels production has largely been marred by photonics-related challenges. To date, most photobioreactor systems make use of artificial sources of illumination thus increasing the overall costs of biomass production. Solar energy, although sustainable and cost-effective, is difficult to manage and control. It also contains other wavelengths which are detrimental to microalgae. Thus, this study sought to make use of spectral filters for optimal outdoor algaculture. Hence, solar energy was used in wastewater-mediated algaculture of native and imported Chlorella sp. under blue, green, red and yellow coloured spectral filters. The native Chlorella sp. had the highest growth rate of 0.892 d−1 and 0.754 d−1 under green and blue coloured filters, respectively. In comparison, the imported Chlorella strain had a growth rate of 0.379 d−1 and 0.267 d−1 under green and blue filters, respectively. Both strains produced high lipid yields under the blue coloured filter, with the native and imported Chlorella strains managing lipid yields of 41.87% dry cell weight (dcw) and 32.29% dcw, respectively. The native Chlorella strain also significantly lowered (p < 0.05) the levels of total nitrogen and ammonium from wastewater with removal efficiencies of 92.17% and 44.60%, respectively, whereas the imported Chlorella strain managed a removal efficiency of 80.81% total nitrogen and 26.10% ammonium under the blue coloured filter. The results indicate that light filtration technology can be used, sustainably, in the simultaneous algaculture of native strains and remediation of wastewater.","PeriodicalId":9076,"journal":{"name":"Biotechnology & Biotechnological Equipment","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2022-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41881416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In vitro and in silico inhibition performance of choline against CYP1A2, CYP2D6 and CYP3A4 胆碱对CYP1A2、CYP2D6和CYP3A4的体外和体外抑制作用

IF 1.4 4区生物学

Biotechnology & Biotechnological Equipment Pub Date : 2022-11-05 DOI: 10.1080/13102818.2022.2144452

E. Mateev, Antoaneta Balkanska-Mitkova, L. Peikova, M. Dimitrova, M. Kondeva-Burdina

{"title":"In vitro and in silico inhibition performance of choline against CYP1A2, CYP2D6 and CYP3A4","authors":"E. Mateev, Antoaneta Balkanska-Mitkova, L. Peikova, M. Dimitrova, M. Kondeva-Burdina","doi":"10.1080/13102818.2022.2144452","DOIUrl":"https://doi.org/10.1080/13102818.2022.2144452","url":null,"abstract":"Abstract Choline is an essential nutrient involved in the synthesis of acetylcholine in the cholinergic neurons. The pharmacokinetic properties of choline are well described; however, there is a lack of data about its activity toward the CYP450 superfamily of enzymes. Therefore, the aim of this study was to conduct in silico and in vitro activity assessments of choline against three major CYP450 isoforms—CYP1A2, CYP2D6, and CYP3A4. Preliminary in silico modeling was performed on a specialized DL-CYP Prediction Server to evaluate the affinity of choline toward the enzymes. The in vitro study contained specific cytochrome P450 isoform inhibitors and substrates (for CYP1A2, CYP2D6, and CYP3A4) to determine the inhibition performance of choline at five different concentrations (0.150 − 1 µmol/L). The potential interactions of choline and CYPs were displayed after molecular docking with Glide (Schrödinger). In addition, induced-fit simulations and binding free energy calculations MM/GBSA (Molecular Mechanics-Generalized Born Surface Area) were applied to predict the accessibility in each CYP isoform. The initial in silico simulations revealed that choline lacks inhibition potency against the aforementioned enzymes. The in vitro evaluations confirmed that choline possessed no effect against CYP1A2; however, at 1 µmol/L choline exerted 22% and 27% blocking capacity against CYP2D6 and CYP3A4, respectively. Furthermore, there was good correlation between the in vitro results and the complexes’ free binding energy recalculations. Overall, the assessments indicated that choline is a weak CYP2D6 and CYP3A4 inhibitor. The latter results should be considered as a source of future unwanted drug–drug interactions.","PeriodicalId":9076,"journal":{"name":"Biotechnology & Biotechnological Equipment","volume":null,"pages":null},"PeriodicalIF":1.4,"publicationDate":"2022-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44572155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0