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Simplified MS2 phage-like particle production including a novel maturation protein internal fusion affinity tag. 简化的MS2噬菌体样颗粒生产，包括一种新的成熟蛋白内部融合亲和力标签。
IF 1.4 4区 生物学
Biotechnology & Biotechnological Equipment Pub Date : 2025-01-01 Epub Date: 2025-02-02 DOI: 10.1080/13102818.2025.2457691
Enos C Kline, Rose Duong, Qin Wang, Barry R Lutz
{"title":"Simplified MS2 phage-like particle production including a novel maturation protein internal fusion affinity tag.","authors":"Enos C Kline, Rose Duong, Qin Wang, Barry R Lutz","doi":"10.1080/13102818.2025.2457691","DOIUrl":"10.1080/13102818.2025.2457691","url":null,"abstract":"<p><p>Phage-like particles (PLPs) are fabricated self-assembling nanoparticles derived from the structural elements of bacteriophages. These particles have biotechnological utility because of the ability to easily modify surface chemistry and compartmentalize nucleic acids or other materials. A consequential implementation of PLPs in diagnostics is as process controls in nucleic acid amplification tests, where control RNAs are packaged within the protein capsid and protected from degradation by RNases in the sample matrix. Key developments in PLP controls have enhanced the packing efficiency of RNAs into particles, reduced the complexity of their plasmid expression systems, and shifted purification from ultracentrifugation to affinity chromatography, producing progressively greater yields with higher purity. Expanding on prior improvements, this study establishes a revised set of plasmid vectors for <i>Emesvirus zinderi</i> (MS2) derived PLPs that streamline vector manipulations for rapid prototyping of new particles, provide validation of an alternative affinity tag for purification, and contributes a high-throughput low-volume spin column purification strategy. These advancements are combined with a novel internal fusion site in MS2 maturation protein A, a passive element of the MS2 capsid in prior PLP designs, that is capable of displaying polypeptides on the particles' surface. The functionality of the chimeric maturation protein's surface display is verified with an affinity tag fusion and subsequent purification. This advancement increases the number of available peptide display sites for the MS2 PLP platform with wide-ranging implications for future applications.</p>","PeriodicalId":9076,"journal":{"name":"Biotechnology & Biotechnological Equipment","volume":"39 1","pages":""},"PeriodicalIF":1.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12393069/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144942299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Polymorphism in SNP G1 of the GDF9 gene associated with reproductive traits in Bulgarian dairy sheep 与保加利亚奶羊繁殖性状相关的 GDF9 基因 SNP G1 的多态性
IF 1.4 4区 生物学
Biotechnology & Biotechnological Equipment Pub Date : 2024-09-10 DOI: 10.1080/13102818.2024.2399939
Ivona Dimitrova, Nevyana Stancheva, Milena Bozhilova-Sakova, Todor Tzonev
{"title":"Polymorphism in SNP G1 of the GDF9 gene associated with reproductive traits in Bulgarian dairy sheep","authors":"Ivona Dimitrova, Nevyana Stancheva, Milena Bozhilova-Sakova, Todor Tzonev","doi":"10.1080/13102818.2024.2399939","DOIUrl":"https://doi.org/10.1080/13102818.2024.2399939","url":null,"abstract":"The Bulgarian Dairy Synthetic Population (BDSP) is the most numerous sheep breed in the country with a fertility of about 150%. Fertility in sheep is mainly determined by several major genes known ...","PeriodicalId":9076,"journal":{"name":"Biotechnology & Biotechnological Equipment","volume":"14 1","pages":""},"PeriodicalIF":1.4,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142247734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Costs of treating type 2 diabetes mellitus and its complications 治疗 2 型糖尿病及其并发症的费用
IF 1.4 4区 生物学
Biotechnology & Biotechnological Equipment Pub Date : 2024-09-09 DOI: 10.1080/13102818.2024.2399941
Nemanja Petrović, Miloš Milosavljević, Refet Gojak, Miralem Đešević, Dragana Lakić, Ivana Stević, Slobodan Janković
{"title":"Costs of treating type 2 diabetes mellitus and its complications","authors":"Nemanja Petrović, Miloš Milosavljević, Refet Gojak, Miralem Đešević, Dragana Lakić, Ivana Stević, Slobodan Janković","doi":"10.1080/13102818.2024.2399941","DOIUrl":"https://doi.org/10.1080/13102818.2024.2399941","url":null,"abstract":"The major driver of the costs of treating patients with diabetes mellitus type 2 (DM2) is its complications, which in developing countries are a consequence of poor glycemic control due to the low ...","PeriodicalId":9076,"journal":{"name":"Biotechnology & Biotechnological Equipment","volume":"99 1","pages":""},"PeriodicalIF":1.4,"publicationDate":"2024-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142206226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A new strategy of multiplex real-time RT-qPCR assay for differentiating Omicron variants from other SARS-CoV-2 lineages 多重实时 RT-qPCR 检测新策略，用于区分 Omicron 变体和其他 SARS-CoV-2 株系
IF 1.4 4区 生物学
Biotechnology & Biotechnological Equipment Pub Date : 2024-08-19 DOI: 10.1080/13102818.2024.2391046
Chen-Sheng Wu, Bo-Han Chen, Hiu-Lo Cheng, Wei-Ming Huang, Tian-Neng Li, Horng-Dar Wang, Chun-Hsien Kuo
{"title":"A new strategy of multiplex real-time RT-qPCR assay for differentiating Omicron variants from other SARS-CoV-2 lineages","authors":"Chen-Sheng Wu, Bo-Han Chen, Hiu-Lo Cheng, Wei-Ming Huang, Tian-Neng Li, Horng-Dar Wang, Chun-Hsien Kuo","doi":"10.1080/13102818.2024.2391046","DOIUrl":"https://doi.org/10.1080/13102818.2024.2391046","url":null,"abstract":"The global dissemination of severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2) Omicron variants has been characterized by a distinct 9-base pair (bp) deletion within the nucleocapsid...","PeriodicalId":9076,"journal":{"name":"Biotechnology & Biotechnological Equipment","volume":"6 1","pages":""},"PeriodicalIF":1.4,"publicationDate":"2024-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142206227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Fast and precise multi-site mutagenesis on linear DNA fragments 对线性 DNA 片段进行快速、精确的多位点诱变
IF 1.4 4区 生物学
Biotechnology & Biotechnological Equipment Pub Date : 2024-08-14 DOI: 10.1080/13102818.2024.2385423
Jie Zhang, Youhui Yang, Zhaoguan Wang, Qian Liu, Peixian Li, Shaohua Kang, Hao Qi
{"title":"Fast and precise multi-site mutagenesis on linear DNA fragments","authors":"Jie Zhang, Youhui Yang, Zhaoguan Wang, Qian Liu, Peixian Li, Shaohua Kang, Hao Qi","doi":"10.1080/13102818.2024.2385423","DOIUrl":"https://doi.org/10.1080/13102818.2024.2385423","url":null,"abstract":"The effectiveness and scalability of site-directed mutagenesis are constrained by the limited number of mutations and the intricate cloning process required for isolation of the target sequence. He...","PeriodicalId":9076,"journal":{"name":"Biotechnology & Biotechnological Equipment","volume":"1 1","pages":""},"PeriodicalIF":1.4,"publicationDate":"2024-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142206229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Analysis of contrast sensitivity in patients implanted with Acunex Vario and LuxSmart extended depth of focus (E-DOF) intraocular lenses (IOLs) 分析植入 Acunex Vario 和 LuxSmart 扩展焦深（E-DOF）眼内透镜（IOL）的患者的对比敏感度
IF 1.4 4区 生物学
Biotechnology & Biotechnological Equipment Pub Date : 2024-08-13 DOI: 10.1080/13102818.2024.2390501
Boryana Irinkova, Irina Kuneva, Iva Petkova
{"title":"Analysis of contrast sensitivity in patients implanted with Acunex Vario and LuxSmart extended depth of focus (E-DOF) intraocular lenses (IOLs)","authors":"Boryana Irinkova, Irina Kuneva, Iva Petkova","doi":"10.1080/13102818.2024.2390501","DOIUrl":"https://doi.org/10.1080/13102818.2024.2390501","url":null,"abstract":"The aim of this study was to track and analyze preoperative and postoperative contrast sensitivity in patients implanted unilaterally or bilaterally with extended depth of focus intraocular lenses....","PeriodicalId":9076,"journal":{"name":"Biotechnology & Biotechnological Equipment","volume":"180 1","pages":""},"PeriodicalIF":1.4,"publicationDate":"2024-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142206228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Implementation of DPYD testing in Bulgarian patients with cancer 在保加利亚癌症患者中实施 DPYD 检测
IF 1.4 4区 生物学
Biotechnology & Biotechnological Equipment Pub Date : 2024-08-07 DOI: 10.1080/13102818.2024.2386073
Nelly Miteva-Marcheva, Hristo Ivanov, Elena Bangieva-Ivanova, Nikola Boyanov, Vili Stoyanova
{"title":"Implementation of DPYD testing in Bulgarian patients with cancer","authors":"Nelly Miteva-Marcheva, Hristo Ivanov, Elena Bangieva-Ivanova, Nikola Boyanov, Vili Stoyanova","doi":"10.1080/13102818.2024.2386073","DOIUrl":"https://doi.org/10.1080/13102818.2024.2386073","url":null,"abstract":"Precision medicine has revolutionized cancer treatment, tailoring interventions based on individual genetic variations. Dihydropyrimidine dehydrogenase gene (DPYD) testing plays a crucial role in p...","PeriodicalId":9076,"journal":{"name":"Biotechnology & Biotechnological Equipment","volume":"10 1","pages":""},"PeriodicalIF":1.4,"publicationDate":"2024-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141947867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
New perspectives into the application of Effective Microorganism (EM) on phytopathogenic fungi: in-vitro antioxidant capacity, bioactive substances and fungicidal efficacy 有效微生物（EM）在植物病原真菌中应用的新视角：体外抗氧化能力、生物活性物质和杀菌效果
IF 1.4 4区 生物学
Biotechnology & Biotechnological Equipment Pub Date : 2024-08-06 DOI: 10.1080/13102818.2024.2387190
Ahmed Abdelkader Saleh, Mohamed Hamed Elsheikh, Fatma Aly El-Nakieb, Sherien Elsayed Sobhy, Sanaa Soliman Kabeil, Elsayed Elsayed Hafez
{"title":"New perspectives into the application of Effective Microorganism (EM) on phytopathogenic fungi: in-vitro antioxidant capacity, bioactive substances and fungicidal efficacy","authors":"Ahmed Abdelkader Saleh, Mohamed Hamed Elsheikh, Fatma Aly El-Nakieb, Sherien Elsayed Sobhy, Sanaa Soliman Kabeil, Elsayed Elsayed Hafez","doi":"10.1080/13102818.2024.2387190","DOIUrl":"https://doi.org/10.1080/13102818.2024.2387190","url":null,"abstract":"During a series of sampling conducted in Nobaria, El-Beheira, Egypt in 2022 and 2023, defoliation disorders were identified in tomato fields. Three soil samples were collected and subjected to fung...","PeriodicalId":9076,"journal":{"name":"Biotechnology & Biotechnological Equipment","volume":"59 1","pages":""},"PeriodicalIF":1.4,"publicationDate":"2024-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141947647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Kawasaki disease with giant coronary aneurysms formation in a 3-month old infant: a case report and review of the literature 一名 3 个月大婴儿的川崎病伴有巨大冠状动脉瘤形成：病例报告和文献综述
IF 1.4 4区 生物学
Biotechnology & Biotechnological Equipment Pub Date : 2024-08-04 DOI: 10.1080/13102818.2024.2386071
Margarita Ganeva, Teodor Vasilev, Katya Temelkova, Desislava Hristova, Anna Dimitrova, Stefan Stefanov
{"title":"Kawasaki disease with giant coronary aneurysms formation in a 3-month old infant: a case report and review of the literature","authors":"Margarita Ganeva, Teodor Vasilev, Katya Temelkova, Desislava Hristova, Anna Dimitrova, Stefan Stefanov","doi":"10.1080/13102818.2024.2386071","DOIUrl":"https://doi.org/10.1080/13102818.2024.2386071","url":null,"abstract":"Kawasaki disease (KD) is an acute systemic vasculitis that affects medium-sized vessels, particularly the coronary arteries. KD predominantly affects children under 5 years of age. Main symptoms in...","PeriodicalId":9076,"journal":{"name":"Biotechnology & Biotechnological Equipment","volume":"11 1","pages":""},"PeriodicalIF":1.4,"publicationDate":"2024-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141947648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Letter to the Editor concerning ‘cost-effectiveness of velmanase alfa vs. bone marrow transplantation or no causal therapy in patients with mild to moderate alpha-mannosidosis’ 致编辑的信，内容涉及 "轻度至中度α-甘露糖苷酶病患者接受 velmanase alfa 与骨髓移植或无因果关系疗法的成本效益对比
IF 1.4 4区 生物学
Biotechnology & Biotechnological Equipment Pub Date : 2024-08-01 DOI: 10.1080/13102818.2024.2386487
A. L. Stevenson, S. Chang-Douglass, P. Kazmierska, A. Benedict, K. Azimpour, P. Dorling
{"title":"Letter to the Editor concerning ‘cost-effectiveness of velmanase alfa vs. bone marrow transplantation or no causal therapy in patients with mild to moderate alpha-mannosidosis’","authors":"A. L. Stevenson, S. Chang-Douglass, P. Kazmierska, A. Benedict, K. Azimpour, P. Dorling","doi":"10.1080/13102818.2024.2386487","DOIUrl":"https://doi.org/10.1080/13102818.2024.2386487","url":null,"abstract":"Published in Biotechnology & Biotechnological Equipment (Vol. 38, No. 1, 2024)","PeriodicalId":9076,"journal":{"name":"Biotechnology & Biotechnological Equipment","volume":"10 1","pages":""},"PeriodicalIF":1.4,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141947650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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