Regeneration (Oxford, England)最新文献_第7页

Angiogenesis is inhibitory for mammalian digit regeneration. 血管生成对哺乳动物手指再生具有抑制作用。

Regeneration (Oxford, England) Pub Date : 2014-10-12 eCollection Date: 2014-06-01 DOI: 10.1002/reg2.24

Ling Yu, Mingquan Yan, Jennifer Simkin, Paulina D Ketcham, Eric Leininger, Manjong Han, Ken Muneoka

{"title":"Angiogenesis is inhibitory for mammalian digit regeneration.","authors":"Ling Yu, Mingquan Yan, Jennifer Simkin, Paulina D Ketcham, Eric Leininger, Manjong Han, Ken Muneoka","doi":"10.1002/reg2.24","DOIUrl":"https://doi.org/10.1002/reg2.24","url":null,"abstract":"The regenerating mouse digit tip is a unique model for investigating blastema formation and epimorphic regeneration in mammals. The blastema is characteristically avascular and we previously reported that blastema expression of a known anti-angiogenic factor gene, Pedf, correlated with a successful regenerative response (Yu, L., Han, M., Yan, M., Lee, E. C., Lee, J. & Muneoka, K. (2010). BMP signaling induces digit regeneration in neonatal mice. Development, 137, 551-559). Here we show that during regeneration Vegfa transcripts are not detected in the blastema but are expressed at the onset of differentiation. Treating the amputation wound with vascular endothelial growth factor enhances angiogenesis but inhibits regeneration. We next tested bone morphogenetic protein 9 (BMP9), another known mediator of angiogenesis, and found that BMP9 is also a potent inhibitor of digit tip regeneration. BMP9 induces Vegfa expression in the digit stump suggesting that regenerative failure is mediated by enhanced angiogenesis. Finally, we show that BMP9 inhibition of regeneration is completely rescued by treatment with pigment epithelium-derived factor. These studies show that precocious angiogenesis is inhibitory for regeneration, and provide compelling evidence that the regulation of angiogenesis is a critical factor in designing therapies aimed at stimulating mammalian regeneration. ","PeriodicalId":90316,"journal":{"name":"Regeneration (Oxford, England)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2014-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/reg2.24","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34738806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 35

Plasticity for axolotl lens regeneration is associated with age-related changes in gene expression. 蝾螈晶状体再生的可塑性与年龄相关的基因表达变化有关。

Regeneration (Oxford, England) Pub Date : 2014-10-12 eCollection Date: 2014-06-01 DOI: 10.1002/reg2.25

Konstantinos Sousounis, Antony T Athippozhy, S Randal Voss, Panagiotis A Tsonis

{"title":"Plasticity for axolotl lens regeneration is associated with age-related changes in gene expression.","authors":"Konstantinos Sousounis, Antony T Athippozhy, S Randal Voss, Panagiotis A Tsonis","doi":"10.1002/reg2.25","DOIUrl":"https://doi.org/10.1002/reg2.25","url":null,"abstract":"Mexican axolotls lose potential for lens regeneration 2 weeks after hatching. We used microarrays to identify differently expressed genes before and after this critical time, using RNA isolated from iris. Over 3700 genes were identified as differentially expressed in response to lentectomy between young (7 days post-hatching) and old (3 months post-hatching) axolotl larvae. Strikingly, many of the genes were only expressed in the early or late iris. Genes that were highly expressed in young iris significantly enriched electron transport chain, transcription, metabolism, and cell cycle gene ontologies, all of which are associated with lens regeneration. In contrast, genes associated with cellular differentiation and tissue maturation were uniquely expressed in old iris. Many of these expression differences strongly suggest that young and old iris samples were collected before and after the spleen became developmentally competent to produce and secrete cells with humoral and innate immunity functions. Our study establishes the axolotl as a powerful model to investigate age-related cellular differentiation and immune system ontogeny within the context of tissue regeneration. ","PeriodicalId":90316,"journal":{"name":"Regeneration (Oxford, England)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2014-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/reg2.25","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34738807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 25

Implication of two different regeneration systems in limb regeneration. 两种不同再生系统在肢体再生中的意义。

Regeneration (Oxford, England) Pub Date : 2014-08-29 eCollection Date: 2014-06-01 DOI: 10.1002/reg2.16

Aki Makanae, Kazumasa Mitogawa, Akira Satoh

引用次数: 15

Temporal and spatial analysis of enteric nervous system regeneration in the sea cucumber Holothuria glaberrima. 光光海参肠神经系统再生的时空分析。

Regeneration (Oxford, England) Pub Date : 2014-08-05 eCollection Date: 2014-06-01 DOI: 10.1002/reg2.15

Karen Tossas, Sunny Qi-Huang, Eugenia Cuyar, Jose E García-Arrarás

{"title":"Temporal and spatial analysis of enteric nervous system regeneration in the sea cucumber Holothuria glaberrima.","authors":"Karen Tossas, Sunny Qi-Huang, Eugenia Cuyar, Jose E García-Arrarás","doi":"10.1002/reg2.15","DOIUrl":"https://doi.org/10.1002/reg2.15","url":null,"abstract":"There is limited information on the regeneration of the enteric nervous system (ENS) following major reconstruction of the digestive tract. We have studied ENS regeneration in the sea cucumber Holothuria glaberrima which undergoes an organogenic process forming a new digestive tract at the tip of the mesentery. Our results show that (1) a degeneration of nerve fibers occurs early in the regeneration process, prior to eventual regeneration; (2) nerve fibers that innervate the regenerating intestine are of extrinsic and intrinsic origin; (3) innervation by extrinsic fibers occurs in a gradient that begins in the proximal area of the regenerate; (4) late events include the appearance of nerve fibers that project from the serosa into the connective tissue and of nerve bundles in the mesothelial layer; (5) neurons and neuroendocrine cells appear early following the formation of the epithelial layers. Our results provide not only a comparative biological approach to study ENS regeneration but also an alternative point of view for the study of enteric neuropathologies and for the innervation of organs made in vitro. ","PeriodicalId":90316,"journal":{"name":"Regeneration (Oxford, England)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2014-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/reg2.15","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34738805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

Probability of Regenerating a Normal Limb After Bite Injury in the Mexican Axolotl (Ambystoma mexicanum). 墨西哥蝾螈(Ambystoma mexicanum)咬伤后再生正常肢体的可能性。

Regeneration (Oxford, England) Pub Date : 2014-06-01 DOI: 10.1002/reg2.17

Sierra Thompson, Laura Muzinic, Christopher Muzinic, Matthew L Niemiller, S Randal Voss

{"title":"Probability of Regenerating a Normal Limb After Bite Injury in the Mexican Axolotl (Ambystoma mexicanum).","authors":"Sierra Thompson, Laura Muzinic, Christopher Muzinic, Matthew L Niemiller, S Randal Voss","doi":"10.1002/reg2.17","DOIUrl":"https://doi.org/10.1002/reg2.17","url":null,"abstract":"Multiple factors are thought to cause limb abnormalities in amphibian populations by altering processes of limb development and regeneration. We examined adult and juvenile axolotls (Ambystoma mexicanum) in the Ambystoma Genetic Stock Center (AGSC) for limb and digit abnormalities to investigate the probability of normal regeneration after bite injury. We observed that 80% of larval salamanders show evidence of bite injury at the time of transition from group housing to solitary housing. Among 717 adult axolotls that were surveyed, which included solitary-housed males and group-housed females, approximately half presented abnormalities, including examples of extra or missing digits and limbs, fused digits, and digits growing from atypical anatomical positions. Bite injury likely explains these limb defects, and not abnormal development, because limbs with normal anatomy regenerated after performing rostral amputations. We infer that only 43% of AGSC larvae will present four anatomically normal looking adult limbs after incurring a bite injury. Our results show regeneration of normal limb anatomy to be less than perfect after bite injury.","PeriodicalId":90316,"journal":{"name":"Regeneration (Oxford, England)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2014-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/reg2.17","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33107646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 15

Ectopic blastema induction by nerve deviation and skin wounding: a new regeneration model in Xenopus laevis. 神经偏离和皮肤损伤诱导非洲爪蟾异位胚源:一种新的再生模式。

Regeneration (Oxford, England) Pub Date : 2014-05-28 eCollection Date: 2014-04-01 DOI: 10.1002/reg2.11

Kazumasa Mitogawa, Ayako Hirata, Miyuki Moriyasu, Aki Makanae, Shinichirou Miura, Tetsuya Endo, Akira Satoh

{"title":"Ectopic blastema induction by nerve deviation and skin wounding: a new regeneration model in Xenopus laevis.","authors":"Kazumasa Mitogawa, Ayako Hirata, Miyuki Moriyasu, Aki Makanae, Shinichirou Miura, Tetsuya Endo, Akira Satoh","doi":"10.1002/reg2.11","DOIUrl":"https://doi.org/10.1002/reg2.11","url":null,"abstract":"Recently, the accessory limb model (ALM) has become an alternative study system for limb regeneration studies in axolotls instead of using an amputated limb. ALM progresses limb regeneration study in axolotls because of its advantages. To apply and/or to compare knowledge in axolotl ALM studies to other vertebrates is a conceivable next step. First, Xenopus laevis, an anuran amphibian, was investigated. A Xenopus frog has hypomorphic regeneration ability. Its regeneration ability has been considered intermediate between that of non-regenerative higher vertebrates and regenerative urodele amphibians. Here, we successfully induced an accessory blastema in Xenopus by skin wounding and rerouting of brachial nerve bundles to the wound site, which is the regular ALM surgery. The induced Xenopus ALM blastemas have limited regenerative potential compared with axolotl ALM blastemas. Comparison of ALM blastemas from species with different regenerative potentials may facilitate the identification of the novel expression programs necessary for the formation of cartilage and other tissues during limb regeneration. ","PeriodicalId":90316,"journal":{"name":"Regeneration (Oxford, England)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2014-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/reg2.11","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34738803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 20

A bioinformatics expert system linking functional data to anatomical outcomes in limb regeneration. 将肢体再生中的功能数据与解剖结果联系起来的生物信息学专家系统。

Regeneration (Oxford, England) Pub Date : 2014-04-01 DOI: 10.1002/reg2.13

Daniel Lobo, Erica B Feldman, Michelle Shah, Taylor J Malone, Michael Levin

{"title":"A bioinformatics expert system linking functional data to anatomical outcomes in limb regeneration.","authors":"Daniel Lobo, Erica B Feldman, Michelle Shah, Taylor J Malone, Michael Levin","doi":"10.1002/reg2.13","DOIUrl":"10.1002/reg2.13","url":null,"abstract":"Amphibians and molting arthropods have the remarkable capacity to regenerate amputated limbs, as described by an extensive literature of experimental cuts, amputations, grafts, and molecular techniques. Despite a rich history of experimental efforts, no comprehensive mechanistic model exists that can account for the pattern regulation observed in these experiments. While bioinformatics algorithms have revolutionized the study of signaling pathways, no such tools have heretofore been available to assist scientists in formulating testable models of large-scale morphogenesis that match published data in the limb regeneration field. Major barriers preventing an algorithmic approach are the lack of formal descriptions for experimental regenerative information and a repository to centralize storage and mining of functional data on limb regeneration. Establishing a new bioinformatics of shape would significantly accelerate the discovery of key insights into the mechanisms that implement complex regeneration. Here, we describe a novel mathematical ontology for limb regeneration to unambiguously encode phenotype, manipulation, and experiment data. Based on this formalism, we present the first centralized formal database of published limb regeneration experiments together with a user-friendly expert system tool to facilitate its access and mining. These resources are freely available for the community and will assist both human biologists and artificial intelligence systems to discover testable, mechanistic models of limb regeneration.","PeriodicalId":90316,"journal":{"name":"Regeneration (Oxford, England)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2014-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4339036/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33419328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of in vitro transcriptional responses of dorsal root ganglia cultured in the presence and absence of blastema cells from regenerating salamander limbs. 蝾螈肢体再生胚母细胞存在和不存在情况下背根神经节体外转录反应的表征。

Regeneration (Oxford, England) Pub Date : 2014-04-01 DOI: 10.1002/reg2.14

Antony Athippozhy, Jeffrey Lehrberg, James R Monaghan, David M Gardiner, S Randal Voss

{"title":"Characterization of in vitro transcriptional responses of dorsal root ganglia cultured in the presence and absence of blastema cells from regenerating salamander limbs.","authors":"Antony Athippozhy, Jeffrey Lehrberg, James R Monaghan, David M Gardiner, S Randal Voss","doi":"10.1002/reg2.14","DOIUrl":"https://doi.org/10.1002/reg2.14","url":null,"abstract":"Abstract During salamander limb regeneration, nerves provide signals that induce the formation of a mass of proliferative cells called the blastema. To better understand these signals, we developed a blastema−dorsal root ganglia (DRG) co‐culture model system to test the hypothesis that nerves differentially express genes in response to cues provided by the blastema. DRG with proximal and distal nerve trunks were isolated from axolotls (Ambystoma mexicanum), cultured for 5 days, and subjected to microarray analysis. Relative to freshly isolated DRG, 1541 Affymetrix probe sets were identified as differentially expressed and many of the predicted genes are known to function in injury and neurodevelopmental responses observed for mammalian DRG. We then cultured 5‐day DRG explants for an additional 5 days with or without co‐cultured blastema cells. On day 10, we identified 27 genes whose expression in cultured DRG was significantly affected by the presence or absence of blastema cells. Overall, our study established a DRG−blastema in vitro culture system and identified candidate genes for future investigations of axon regrowth, nerve−blastema signaling, and neural regulation of limb regeneration.","PeriodicalId":90316,"journal":{"name":"Regeneration (Oxford, England)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2014-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/reg2.14","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33111822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14

Rapid clearance of epigenetic protein reporters from wound edge cells in Drosophila larvae does not depend on the JNK or PDGFR/VEGFR signaling pathways. 果蝇幼虫创口边缘细胞中表观遗传蛋白报告蛋白的快速清除不依赖于JNK或PDGFR/VEGFR信号通路。

Regeneration (Oxford, England) Pub Date : 2014-04-01 DOI: 10.1002/reg2.12

Aimee E Anderson, Michael J Galko

{"title":"Rapid clearance of epigenetic protein reporters from wound edge cells in Drosophila larvae does not depend on the JNK or PDGFR/VEGFR signaling pathways.","authors":"Aimee E Anderson, Michael J Galko","doi":"10.1002/reg2.12","DOIUrl":"https://doi.org/10.1002/reg2.12","url":null,"abstract":"The drastic cellular changes required for epidermal cells to dedifferentiate and become motile during wound closure are accompanied by changes in gene transcription, suggesting corresponding alterations in chromatin. However, the epigenetic changes that underlie wound-induced transcriptional programs remain poorly understood partly because a comprehensive study of epigenetic factor expression during wound healing has not been practical. To determine which chromatin modifying factors might contribute to wound healing, we screened publicly available fluorescently-tagged reporter lines in Drosophila for altered expression at the wound periphery during healing. Thirteen reporters tagging seven different proteins showed strongly diminished expression at the wound edge. Three downregulated proteins, Osa, Kismet, and Spt6, are generally associated with active chromatin, while four others, Sin3A, Sap130, Mi-2, and Mip120, are associated with repressed chromatin. In all cases reporter down regulation was independent of the Jun N-terminal Kinase and Pvr pathways, suggesting that novel signals control reporter clearance. Taken together, our results suggest that clearance of chromatin modifying factors may enable wound edge cells to rapidly and comprehensively change their transcriptional state following tissue damage.","PeriodicalId":90316,"journal":{"name":"Regeneration (Oxford, England)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2014-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/reg2.12","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32578051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Experimentally induced metamorphosis in axolotls reduces regenerative rate and fidelity. 实验诱导的蝾螈变态会降低再生速率和保真度。

Regeneration (Oxford, England) Pub Date : 2014-02-20 eCollection Date: 2014-02-01 DOI: 10.1002/reg2.8

James R Monaghan, Adrian C Stier, François Michonneau, Matthew D Smith, Bret Pasch, Malcolm Maden, Ashley W Seifert

{"title":"Experimentally induced metamorphosis in axolotls reduces regenerative rate and fidelity.","authors":"James R Monaghan, Adrian C Stier, François Michonneau, Matthew D Smith, Bret Pasch, Malcolm Maden, Ashley W Seifert","doi":"10.1002/reg2.8","DOIUrl":"https://doi.org/10.1002/reg2.8","url":null,"abstract":"While most tetrapods are unable to regenerate severed body parts, amphibians display a remarkable ability to regenerate an array of structures. Frogs can regenerate appendages as larva, but they lose this ability around metamorphosis. In contrast, salamanders regenerate appendages as larva, juveniles, and adults. However, the extent to which fundamental traits (e.g., metamorphosis, body size, aging, etc.) restrict regenerative ability remains contentious. Here we utilize the ability of normally paedomorphic adult axolotls (Ambystoma mexicanum) to undergo induced metamorphosis by thyroxine exposure to test how metamorphosis and body size affects regeneration in age-matched paedomorphic and metamorphic individuals. We show that body size does not affect regeneration in adult axolotls, but metamorphosis causes a twofold reduction in regeneration rate, and lead to carpal and digit malformations. Furthermore, we find evidence that metamorphic blastemal cells may take longer to traverse the cell cycle and display a lower proliferative rate. This study identifies the axolotl as a powerful system to study how metamorphosis restricts regeneration independently of developmental stage, body size, and age; and more broadly how metamorphosis affects tissue-specific changes.","PeriodicalId":90316,"journal":{"name":"Regeneration (Oxford, England)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2014-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/reg2.8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34737523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 69