Regeneration (Oxford, England)最新文献_第4页

De novo assembly of plant body plan: a step ahead of Deadpool. 植物身体计划的全新组装：比 "死侍 "更进一步。

Regeneration (Oxford, England) Pub Date : 2016-10-28 eCollection Date: 2016-08-01 DOI: 10.1002/reg2.68

Abdul Kareem, Dhanya Radhakrishnan, Yash Sondhi, Mohammed Aiyaz, Merin V Roy, Kaoru Sugimoto, Kalika Prasad

{"title":"De novo assembly of plant body plan: a step ahead of Deadpool.","authors":"Abdul Kareem, Dhanya Radhakrishnan, Yash Sondhi, Mohammed Aiyaz, Merin V Roy, Kaoru Sugimoto, Kalika Prasad","doi":"10.1002/reg2.68","DOIUrl":"10.1002/reg2.68","url":null,"abstract":"While in the movie Deadpool it is possible for a human to recreate an arm from scratch, in reality plants can even surpass that. Not only can they regenerate lost parts, but also the whole plant body can be reborn from a few existing cells. Despite the decades old realization that plant cells possess the ability to regenerate a complete shoot and root system, it is only now that the underlying mechanisms are being unraveled. De novo plant regeneration involves the initiation of regenerative mass, acquisition of the pluripotent state, reconstitution of stem cells and assembly of regulatory interactions. Recent studies have furthered our understanding on the making of a complete plant system in the absence of embryonic positional cues. We review the recent studies probing the molecular mechanisms of de novo plant regeneration in response to external inductive cues and our current knowledge of direct reprogramming of root to shoot and vice versa. We further discuss how de novo regeneration can be exploited to meet the demands of green culture industries and to serve as a general model to address the fundamental questions of regeneration across the plant kingdom.","PeriodicalId":90316,"journal":{"name":"Regeneration (Oxford, England)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5084358/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"51269790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Externally imposed electric field enhances plant root tip regeneration. 外加电场对植物根尖再生有促进作用。

Regeneration (Oxford, England) Pub Date : 2016-08-20 eCollection Date: 2016-06-01 DOI: 10.1002/reg2.59

Nicolas Kral, Alexandra Hanna Ougolnikova, Giovanni Sena

引用次数: 25

The NuRD complex component p66 suppresses photoreceptor neuron regeneration in planarians. NuRD复合物p66抑制涡虫的光感受器神经元再生。

Regeneration (Oxford, England) Pub Date : 2016-08-18 eCollection Date: 2016-06-01 DOI: 10.1002/reg2.58

Constanza Vásquez-Doorman, Christian P Petersen

{"title":"The NuRD complex component p66 suppresses photoreceptor neuron regeneration in planarians.","authors":"Constanza Vásquez-Doorman, Christian P Petersen","doi":"10.1002/reg2.58","DOIUrl":"https://doi.org/10.1002/reg2.58","url":null,"abstract":"Regeneration involves precise control of cell fate to produce an appropriate complement of tissues formed within a blastema. Several chromatin-modifying complexes have been identified as required for regeneration in planarians, but it is unclear whether this class of molecules uniformly promotes the production of differentiated cells. We identify a function for p66, encoding a DNA-binding protein component of the NuRD (nucleosome remodeling and deacetylase) complex, as well as the chromodomain helicase chd4, in suppressing production of photoreceptor neurons (PRNs) in planarians. This suppressive effect appeared restricted to PRNs because p66 inhibition did not influence numbers of eye pigment cup cells (PCCs) and decreased numbers of brain neurons and epidermal progenitors. PRNs from p66(RNAi) animals differentiated with some abnormalities but nonetheless produced arrestin+ projections to the brain. p66 inhibition produced excess ovo+otxA+ PRN progenitors without affecting numbers of ovo+otxA- PCC progenitors, and ovo and otxA were each required for the p66(RNAi) excess PRN phenotype. Together these results suggest that p66 acts through the NuRD complex to suppress PRN production by limiting expression of lineage-specific transcription factors.","PeriodicalId":90316,"journal":{"name":"Regeneration (Oxford, England)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/reg2.58","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34426437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 15

The cellular and molecular mechanisms of tissue repair and regeneration as revealed by studies in Xenopus 非洲爪蟾组织修复和再生的细胞和分子机制研究

Regeneration (Oxford, England) Pub Date : 2016-08-01 DOI: 10.1002/reg2.69

Jingjing Li, Siwei Zhang, E. Amaya

引用次数: 28

Temporal regulation of planarian eye regeneration 涡虫眼再生的时间调节

Regeneration (Oxford, England) Pub Date : 2016-08-01 DOI: 10.1002/reg2.61

Michelle E. Deochand, T. Birkholz, Wendy S. Beane

{"title":"Temporal regulation of planarian eye regeneration","authors":"Michelle E. Deochand, T. Birkholz, Wendy S. Beane","doi":"10.1002/reg2.61","DOIUrl":"https://doi.org/10.1002/reg2.61","url":null,"abstract":"Abstract While tissue regeneration is typically studied using standard injury models, in nature injuries vary greatly in the amount and location of tissues lost. Planarians have the unique ability to regenerate from many different injuries (including from tiny fragments with no brain), allowing us to study the effects of different injuries on regeneration timelines. We followed the timing of regeneration for one organ, the eye, after multiple injury types that involved tissue loss (single‐ and double‐eye ablation, and decapitation) in Schmidtea mediterranea. Our data reveal that the timing of regeneration remained constant despite changing injury parameters. Optic tissue regrowth, nerve re‐innervation, and functional recovery were similar between injury types (even when the animal was simultaneously regrowing its brain). Changes in metabolic rate (i.e., starving vs. fed regenerates) also had no effect on regeneration timelines. In addition, our data suggest there may exist a role for optic nerve degeneration following eye ablation. Our results suggest that the temporal regulation of planarian eye regeneration is tightly controlled and resistant to variations in injury type.","PeriodicalId":90316,"journal":{"name":"Regeneration (Oxford, England)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/reg2.61","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"51269622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

Go ahead, grow a head! A planarian's guide to anterior regeneration. 来吧，长个脑袋!涡虫前肢再生指南。

Regeneration (Oxford, England) Pub Date : 2016-06-24 eCollection Date: 2016-06-01 DOI: 10.1002/reg2.56

Suthira Owlarn, Kerstin Bartscherer

引用次数: 53

The relationship between growth and pattern formation. 生长与模式形成之间的关系

Regeneration (Oxford, England) Pub Date : 2016-04-28 eCollection Date: 2016-04-01 DOI: 10.1002/reg2.55

Susan V Bryant, David M Gardiner

{"title":"The relationship between growth and pattern formation.","authors":"Susan V Bryant, David M Gardiner","doi":"10.1002/reg2.55","DOIUrl":"10.1002/reg2.55","url":null,"abstract":"Successful development depends on the creation of spatial gradients of transcription factors within developing fields, and images of graded distributions of gene products populate the pages of developmental biology journals. Therefore the challenge is to understand how the graded levels of intracellular transcription factors are generated across fields of cells. We propose that transcription factor gradients are generated as a result of an underlying gradient of cell cycle lengths. Very long cell cycles will permit accumulation of a high level of a gene product encoded by a large transcription unit, whereas shorter cell cycles will permit progressively fewer transcripts to be completed due to gating of transcription by the cell cycle. We also propose that the gradients of cell cycle lengths are generated by gradients of extracellular morphogens/growth factors. The model of cell cycle gated transcriptional regulation brings focus back to the functional role of morphogens as cell cycle regulators, and proposes a specific and testable mechanism by which morphogens, in their roles as growth factors (how they were originally discovered), also determine cell fate. ","PeriodicalId":90316,"journal":{"name":"Regeneration (Oxford, England)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4895327/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34737335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Physiological controls of large-scale patterning in planarian regeneration: a molecular and computational perspective on growth and form. 刨形动物再生过程中大规模模式化的生理学控制：从分子和计算角度看生长和形态。

Regeneration (Oxford, England) Pub Date : 2016-04-28 eCollection Date: 2016-04-01 DOI: 10.1002/reg2.54

Fallon Durant, Daniel Lobo, Jennifer Hammelman, Michael Levin

{"title":"Physiological controls of large-scale patterning in planarian regeneration: a molecular and computational perspective on growth and form.","authors":"Fallon Durant, Daniel Lobo, Jennifer Hammelman, Michael Levin","doi":"10.1002/reg2.54","DOIUrl":"10.1002/reg2.54","url":null,"abstract":"Planaria are complex metazoans that repair damage to their bodies and cease remodeling when a correct anatomy has been achieved. This model system offers a unique opportunity to understand how large-scale anatomical homeostasis emerges from the activities of individual cells. Much progress has been made on the molecular genetics of stem cell activity in planaria. However, recent data also indicate that the global pattern is regulated by physiological circuits composed of ionic and neurotransmitter signaling. Here, we overview the multi-scale problem of understanding pattern regulation in planaria, with specific focus on bioelectric signaling via ion channels and gap junctions (electrical synapses), and computational efforts to extract explanatory models from functional and molecular data on regeneration. We present a perspective that interprets results in this fascinating field using concepts from dynamical systems theory and computational neuroscience. Serving as a tractable nexus between genetic, physiological, and computational approaches to pattern regulation, planarian pattern homeostasis harbors many deep insights for regenerative medicine, evolutionary biology, and engineering. ","PeriodicalId":90316,"journal":{"name":"Regeneration (Oxford, England)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4895326/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34737336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Alternative flow cytometry strategies to analyze stem cells and cell death in planarians. 流式细胞术分析涡虫干细胞和细胞死亡的替代策略。

Regeneration (Oxford, England) Pub Date : 2016-03-16 eCollection Date: 2016-04-01 DOI: 10.1002/reg2.53

Tanuja Harshani Peiris, Marcos E García-Ojeda, Néstor J Oviedo

{"title":"Alternative flow cytometry strategies to analyze stem cells and cell death in planarians.","authors":"Tanuja Harshani Peiris, Marcos E García-Ojeda, Néstor J Oviedo","doi":"10.1002/reg2.53","DOIUrl":"https://doi.org/10.1002/reg2.53","url":null,"abstract":"Planarians possess remarkable stem cell populations that continuously support cellular turnover and are instrumental in the regeneration of tissues upon injury. Cellular turnover and tissue regeneration in planarians rely on the proper integration of local and systemic signals that regulate cell proliferation and cell death. Thus, understanding the signals controlling cellular proliferation and cell death in planarians could provide valuable insights for maintenance of adult body homeostasis and the biology of regeneration. Flow cytometry techniques have been utilized widely to identify, isolate, and characterize planarian stem cell populations. We developed alternative flow cytometry strategies that reduce the number of reagents and the time of sample preparation to analyze stem cells and cell death in planarians. The sensitivity of these methods is validated with functional studies using RNA interference and treatment with γ irradiation or stressful conditions that are known to trigger cell death. Altogether, we provide a community resource intended to minimize adverse effects during ex vivo studies of stem cells and cell death in planarians.","PeriodicalId":90316,"journal":{"name":"Regeneration (Oxford, England)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/reg2.53","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34484542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

Planarian brain regeneration as a model system for developmental neurotoxicology. 作为发育神经毒理学模型系统的涡虫脑再生。

Regeneration (Oxford, England) Pub Date : 2016-03-15 eCollection Date: 2016-04-01 DOI: 10.1002/reg2.52

Danielle Hagstrom, Olivier Cochet-Escartin, Eva-Maria S Collins

引用次数: 62