Regeneration (Oxford, England)最新文献_第3页

The periosteal requirement and temporal dynamics of BMP2-induced middle phalanx regeneration in the adult mouse. bmp2诱导成年小鼠中指骨再生的骨膜需求和时间动力学。

Regeneration (Oxford, England) Pub Date : 2017-08-20 eCollection Date: 2017-06-01 DOI: 10.1002/reg2.81

Lindsay A Dawson, Ling Yu, Mingquan Yan, Luis Marrero, Paula P Schanes, Connor Dolan, Maegan Pela, Britta Petersen, Manjong Han, Ken Muneoka

{"title":"The periosteal requirement and temporal dynamics of BMP2-induced middle phalanx regeneration in the adult mouse.","authors":"Lindsay A Dawson, Ling Yu, Mingquan Yan, Luis Marrero, Paula P Schanes, Connor Dolan, Maegan Pela, Britta Petersen, Manjong Han, Ken Muneoka","doi":"10.1002/reg2.81","DOIUrl":"https://doi.org/10.1002/reg2.81","url":null,"abstract":"Regeneration of mammalian limbs is restricted to amputation of the distal digit tip, the terminal phalanx (P3). The adjacent skeletal element, the middle phalanx (P2), has emerged as a model system to investigate regenerative failure and as a site to test approaches aimed at enhancing regeneration. We report that exogenous application of bone morphogenetic protein 2 (BMP2) stimulates the formation of a transient cartilaginous callus distal to the amputation plane that mediates the regeneration of the amputated P2 bone. BMP2 initiates a significant regeneration response during the periosteal-derived cartilaginous healing phase of P2 bone repair, yet fails to induce regeneration in the absence of periosteal tissue, or after boney callus formation. We provide evidence that a temporal component exists in the induced regeneration of P2 that we define as the \"regeneration window.\" In this window, cells are transiently responsive to BMP2 after the amputation injury. Simple re-injury of the healed P2 stump acts to reinitiate endogenous bone repair, complete with periosteal chondrogenesis, thus reopening the \"regeneration window\" and thereby recreating a regeneration-permissive environment that is responsive to exogenous BMP2 treatment.","PeriodicalId":90316,"journal":{"name":"Regeneration (Oxford, England)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/reg2.81","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35470594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 29

Propofol (2,6-diisopropylphenol) is an applicable immersion anesthetic in the axolotl with potential uses in hemodynamic and neurophysiological experiments. 异丙酚(2,6-二异丙基酚)是一种适用于美西螈的浸泡麻醉剂，在血液动力学和神经生理学实验中具有潜在的用途。

Regeneration (Oxford, England) Pub Date : 2017-07-27 eCollection Date: 2017-06-01 DOI: 10.1002/reg2.80

Mathias Møller Thygesen, Mikkel Mylius Rasmussen, Jesper Guldsmed Madsen, Michael Pedersen, Henrik Lauridsen

{"title":"Propofol (2,6-diisopropylphenol) is an applicable immersion anesthetic in the axolotl with potential uses in hemodynamic and neurophysiological experiments.","authors":"Mathias Møller Thygesen, Mikkel Mylius Rasmussen, Jesper Guldsmed Madsen, Michael Pedersen, Henrik Lauridsen","doi":"10.1002/reg2.80","DOIUrl":"https://doi.org/10.1002/reg2.80","url":null,"abstract":"The Mexican axolotl (Ambystoma mexicanum) is an important model species in regenerative biology. Traditionally, axolotls are anesthetized using benzocaine or MS-222, both of which act to inhibit voltage gated sodium channels thereby preventing action potential propagation. In some neurophysiological experiments this is not desirable; therefore we tested propofol as an alternative anesthetic in the axolotl. We evaluated benzocaine, MS-222, and propofol's cardiovascular effects, effects on action potential propagation in the spinal cord, and gross limb regenerative effects. We found that propofol is applicable as a general anesthetic in the axolotl allowing for neurophysiological experiments and yielding a stable anesthesia with significantly less cardiovascular effect than both benzocaine and MS-222. Additionally, propofol did not affect gross limb regeneration. In conclusion we suggest the consideration of propofol as an alternative immersion anesthetic to benzocaine and MS-222.","PeriodicalId":90316,"journal":{"name":"Regeneration (Oxford, England)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/reg2.80","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35470592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 15

Planarian regeneration in space: Persistent anatomical, behavioral, and bacteriological changes induced by space travel. 太空中的涡虫再生:太空旅行引起的持续解剖学、行为学和细菌学变化。

Regeneration (Oxford, England) Pub Date : 2017-06-13 eCollection Date: 2017-04-01 DOI: 10.1002/reg2.79

Junji Morokuma, Fallon Durant, Katherine B Williams, Joshua M Finkelstein, Douglas J Blackiston, Twyman Clements, David W Reed, Michael Roberts, Mahendra Jain, Kris Kimel, Sunia A Trauger, Benjamin E Wolfe, Michael Levin

{"title":"Planarian regeneration in space: Persistent anatomical, behavioral, and bacteriological changes induced by space travel.","authors":"Junji Morokuma, Fallon Durant, Katherine B Williams, Joshua M Finkelstein, Douglas J Blackiston, Twyman Clements, David W Reed, Michael Roberts, Mahendra Jain, Kris Kimel, Sunia A Trauger, Benjamin E Wolfe, Michael Levin","doi":"10.1002/reg2.79","DOIUrl":"https://doi.org/10.1002/reg2.79","url":null,"abstract":"Regeneration is regulated not only by chemical signals but also by physical processes, such as bioelectric gradients. How these may change in the absence of the normal gravitational and geomagnetic fields is largely unknown. Planarian flatworms were moved to the International Space Station for 5 weeks, immediately after removing their heads and tails. A control group in spring water remained on Earth. No manipulation of the planaria occurred while they were in orbit, and space-exposed worms were returned to our laboratory for analysis. One animal out of 15 regenerated into a double-headed phenotype-normally an extremely rare event. Remarkably, amputating this double-headed worm again, in plain water, resulted again in the double-headed phenotype. Moreover, even when tested 20 months after return to Earth, the space-exposed worms displayed significant quantitative differences in behavior and microbiome composition. These observations may have implications for human and animal space travelers, but could also elucidate how microgravity and hypomagnetic environments could be used to trigger desired morphological, neurological, physiological, and bacteriomic changes for various regenerative and bioengineering applications.","PeriodicalId":90316,"journal":{"name":"Regeneration (Oxford, England)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/reg2.79","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35091598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 22

Regulation of dorso-ventral polarity by the nerve cord during annelid regeneration: A review of experimental evidence. 环节动物再生过程中神经索对背-腹侧极性的调节:实验证据综述。

Regeneration (Oxford, England) Pub Date : 2017-06-13 eCollection Date: 2017-04-01 DOI: 10.1002/reg2.78

Bénoni Boilly, Yolande Boilly-Marer, Alexandra E Bely

{"title":"Regulation of dorso-ventral polarity by the nerve cord during annelid regeneration: A review of experimental evidence.","authors":"Bénoni Boilly, Yolande Boilly-Marer, Alexandra E Bely","doi":"10.1002/reg2.78","DOIUrl":"10.1002/reg2.78","url":null,"abstract":"Abstract An important goal for understanding regeneration is determining how polarity is conferred to the regenerate. Here we review findings in two groups of polychaete annelids that implicate the ventral nerve cord in assigning dorso‐ventral polarity, and specifically ventral identity, to the regenerate. In nereids, surgical manipulations indicate that parapodia develop where dorsal and ventral body wall territories contact. Without a nerve cord at the wound site, the regenerate differentiates no evident polarity (with no parapodia) and only dorsal identity, while with two nerve cords the regenerate develops a twinned dorso‐ventral axis (with four parapodia per segment instead of the normal two). In sabellids, a striking natural dorso‐ventral inversion in parapodial morphology occurs along the body axis and this inversion is morphologically correlated with the position of the nerve cord. Parapodial inversion also occurs in segments in which the nerve cord has been removed, even without any segment amputation. Together, these data strongly support a role for the nerve cord in annelid dorso‐ventral pattern regulation, with the nerve cord conferring ventral identity.","PeriodicalId":90316,"journal":{"name":"Regeneration (Oxford, England)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/reg2.78","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35091595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 19

Macrophages and fibroblasts during inflammation and tissue repair in models of organ regeneration. 巨噬细胞和成纤维细胞在器官再生模型炎症和组织修复中的作用。

Regeneration (Oxford, England) Pub Date : 2017-06-06 eCollection Date: 2017-04-01 DOI: 10.1002/reg2.77

Anthony L Mescher

引用次数: 151

Fibroblast reticular cells engineer a blastema extracellular network during digit tip regeneration in mice. 成纤维细胞网状细胞在小鼠指尖再生过程中形成囊胚细胞外网络。

Regeneration (Oxford, England) Pub Date : 2017-05-03 eCollection Date: 2017-04-01 DOI: 10.1002/reg2.75

Luis Marrero, Jennifer Simkin, Mimi Sammarco, Ken Muneoka

{"title":"Fibroblast reticular cells engineer a blastema extracellular network during digit tip regeneration in mice.","authors":"Luis Marrero, Jennifer Simkin, Mimi Sammarco, Ken Muneoka","doi":"10.1002/reg2.75","DOIUrl":"https://doi.org/10.1002/reg2.75","url":null,"abstract":"The regeneration blastema which forms following amputation of the mouse digit tip is composed of undifferentiated cells bound together by an organized network of fibers. A monoclonal antibody (ER-TR7) that identifies extracellular matrix (ECM) fibers produced by fibroblast reticular cells during lymphoid organogenesis was used to characterize the ECM of the digit, the blastema, and the regenerate. Digit fibroblast reticular cells produce an ER-TR7+ ECM network associated with different tissues and represent a subset of loose connective tissue fibroblasts. During blastema formation there is an upregulation of matrix production that returns to its pre-existing level and anatomical pattern in the endpoint regenerate. Co-localization studies demonstrate a strong spatial correlation between the ER-TR7 antigen and collagen type III (COL3) in histological sections. ER-TR7 and COL3 are co-induced in cultured digit fibroblasts following treatment with tumor necrosis factor alpha and a lymphotoxin beta receptor agonist. These results provide an initial characterization of the ECM during digit regeneration and identify a subpopulation of fibroblasts involved in producing the blastema provisional matrix that is remodeled during the regeneration response.","PeriodicalId":90316,"journal":{"name":"Regeneration (Oxford, England)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/reg2.75","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35091596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 24

JAK-STAT pathway activation in response to spinal cord injury in regenerative and non-regenerative stages of Xenopus laevis. 爪蟾再生期和非再生期脊髓损伤时的 JAK-STAT 通路激活。

Regeneration (Oxford, England) Pub Date : 2017-03-14 eCollection Date: 2017-02-01 DOI: 10.1002/reg2.74

Victor S Tapia, Mauricio Herrera-Rojas, Juan Larrain

{"title":"JAK-STAT pathway activation in response to spinal cord injury in regenerative and non-regenerative stages of Xenopus laevis.","authors":"Victor S Tapia, Mauricio Herrera-Rojas, Juan Larrain","doi":"10.1002/reg2.74","DOIUrl":"10.1002/reg2.74","url":null,"abstract":"Xenopus laevis tadpoles can regenerate the spinal cord after injury but this capability is lost during metamorphosis. Comparative studies between pre-metamorphic and metamorphic Xenopus stages can aid towards understanding the molecular mechanisms of spinal cord regeneration. Analysis of a previous transcriptome-wide study suggests that, in response to injury, the JAK-STAT pathway is differentially activated in regenerative and non-regenerative stages. We characterized the activation of the JAK-STAT pathway and found that regenerative tadpoles have an early and transient activation. In contrast, the non-regenerative stages have a delayed and sustained activation of the pathway. We found that STAT3 is activated in response to injury mainly in Sox2/3+ ependymal cells, motoneurons and sensory neurons. Finally, to study the role of temporal activation we generated a transgenic line to express a constitutively active version of STAT3. The sustained activation of the JAK-STAT pathway in regenerative tadpoles reduced the expression of pro-neurogenic genes normally upregulated in response to spinal cord injury, suggesting that activation of the JAK-STAT pathway modulates the fate of neural progenitors.","PeriodicalId":90316,"journal":{"name":"Regeneration (Oxford, England)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5350081/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34832938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Epigenetic memory and cell fate reprogramming in plants. 植物的表观遗传记忆和细胞命运重编程。

Regeneration (Oxford, England) Pub Date : 2017-02-01 DOI: 10.1002/reg2.73

Kenneth D Birnbaum, François Roudier

引用次数: 45

Plant grafting: insights into tissue regeneration. 植物嫁接:组织再生的见解。

Regeneration (Oxford, England) Pub Date : 2016-12-21 eCollection Date: 2017-02-01 DOI: 10.1002/reg2.71

Charles W Melnyk

{"title":"Plant grafting: insights into tissue regeneration.","authors":"Charles W Melnyk","doi":"10.1002/reg2.71","DOIUrl":"https://doi.org/10.1002/reg2.71","url":null,"abstract":"For millennia, people have cut and joined different plants together through a process known as grafting. The severed tissues adhere, the cells divide and the vasculature differentiates through a remarkable process of regeneration between two genetically distinct organisms as they become one. Grafting is becoming increasingly important in horticulture where it provides an efficient means for asexual propagation. Grafting also combines desirable roots and shoots to generate chimeras that are more vigorous, more pathogen resistant and more abiotic stress resistant. Thus, it presents an elegant and efficient way to improve plant productivity in vegetables and trees using traditional techniques. Despite this horticultural importance, we are only beginning to understand how plants regenerate tissues at the graft junction. By understanding grafting better, we can shed light on fundamental regeneration pathways and the basis for self/non-self recognition. We can also better understand why many plants efficiently graft whereas others cannot, with the goal of improving grafting so as to broaden the range of grafted plants to create even more desirable chimeras. Here, I review the latest findings describing how plants graft and provide insight into future directions in this emerging field.","PeriodicalId":90316,"journal":{"name":"Regeneration (Oxford, England)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/reg2.71","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34832969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 139

Major Development in Regeneration. 再生的主要发展。

Regeneration (Oxford, England) Pub Date : 2016-10-28 DOI: 10.1002/reg2.72

E. Amaya, S. Bryant

引用次数: 0