发布求助
文献互助 智能选刊 最新文献

British biotechnology journal最新文献

筛选
英文 中文
Isolation and Purification of Lipase from the Midgut of Fifth Instar Larvae of Antheraea mylitta drury 柞蚕5龄幼虫中肠脂肪酶的分离纯化
British biotechnology journal Pub Date : 2016-01-10 DOI: 10.9734/BBJ/2016/24442
Lakshmi Marepally, G. Benarjee
{"title":"Isolation and Purification of Lipase from the Midgut of Fifth Instar Larvae of Antheraea mylitta drury","authors":"Lakshmi Marepally, G. Benarjee","doi":"10.9734/BBJ/2016/24442","DOIUrl":"https://doi.org/10.9734/BBJ/2016/24442","url":null,"abstract":"Lipases obtained from healthy and pebrinised larvae were purified by 45-85% (NH4) 2SO 4 fractionation followed by Sephadex S-100 gel filtration and CM-Sepharose. In both the samples final enzyme purification reported was 19.02 and 17.7 folds(magnitude) and the recovery of final purified enzyme was 19.32% and 17.14% with a specific activity of 7.87 and 7.52 µmol/min/mg. Results also show that activity of purified lipase was highest at pH 8 in both the samples. Highest lipase activity was recorded between 37°C to 40°C tem perature and lipase activity was maximum at 37°C temperature in healthy sample and 38°C tempe rature in pebrinised sample. The enzyme activity reduced with addition of NaCl, Urea and MgCl 2 whereas EDTA and CaCl 2 increased the activity. The molecular weight of the purified enzyme was 30 kDa as determined by SDSpolyacrylamide gel electrophoresis. Aim: The present study was conducted to isolate, purify and characterize lipases from the midgut of both healthy and pebrinised fifth instar larvae of Antheraea mylitta drury . Study Design: Study involves dissection of midgut from the fifth instar larvae of fourth day of both healthy and pebrinised larvae, Lipases obtained from both the samples were purified by 45-85%","PeriodicalId":90120,"journal":{"name":"British biotechnology journal","volume":"12 1","pages":"1-9"},"PeriodicalIF":0.0,"publicationDate":"2016-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71164951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Antioxidant Property and Phenolic Content of Anthocleista vogelii Root Ethanolic Extract and Fractions 石菖蒲根乙醇提取物及馏分的抗氧化性能及酚类物质含量
British biotechnology journal Pub Date : 2016-01-10 DOI: 10.9734/BBJ/2016/27016
R. Sunday, O. Ilesanmi, E. Obuotor
{"title":"Antioxidant Property and Phenolic Content of Anthocleista vogelii Root Ethanolic Extract and Fractions","authors":"R. Sunday, O. Ilesanmi, E. Obuotor","doi":"10.9734/BBJ/2016/27016","DOIUrl":"https://doi.org/10.9734/BBJ/2016/27016","url":null,"abstract":"","PeriodicalId":90120,"journal":{"name":"British biotechnology journal","volume":"14 1","pages":"1-8"},"PeriodicalIF":0.0,"publicationDate":"2016-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71165359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Evaluation of Virulence of Tanzanian Strains of Fowlpox and Pigeonpox Viruses in Chickens 坦桑尼亚禽痘和鸽痘病毒株对鸡的毒力评价
British biotechnology journal Pub Date : 2016-01-10 DOI: 10.9734/bbj/2016/20150
S. Masola, A. Mzula, C. Kasanga, P. Wambura
{"title":"Evaluation of Virulence of Tanzanian Strains of Fowlpox and Pigeonpox Viruses in Chickens","authors":"S. Masola, A. Mzula, C. Kasanga, P. Wambura","doi":"10.9734/bbj/2016/20150","DOIUrl":"https://doi.org/10.9734/bbj/2016/20150","url":null,"abstract":"","PeriodicalId":90120,"journal":{"name":"British biotechnology journal","volume":"10 1","pages":"1-10"},"PeriodicalIF":0.0,"publicationDate":"2016-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71163614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Development of an Efficient Plant Regeneration System of Field Mustard (Brassica campestris) 芥菜高效植株再生体系的研制
British biotechnology journal Pub Date : 2016-01-10 DOI: 10.9734/bbj/2016/21529
Kishor Kumar Sarker, F. Monshi, Sayeda Sultana, Delara Akhter, M. Bhuiyan
{"title":"Development of an Efficient Plant Regeneration System of Field Mustard (Brassica campestris)","authors":"Kishor Kumar Sarker, F. Monshi, Sayeda Sultana, Delara Akhter, M. Bhuiyan","doi":"10.9734/bbj/2016/21529","DOIUrl":"https://doi.org/10.9734/bbj/2016/21529","url":null,"abstract":"","PeriodicalId":90120,"journal":{"name":"British biotechnology journal","volume":"12 1","pages":"1-10"},"PeriodicalIF":0.0,"publicationDate":"2016-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71163823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Biovalorization of Olive Mill Waste Water for the Production of Single Cell Protein from Saccharomyces cerevisiae, Candida utilis and Pleurotus ostreatus 橄榄厂废水在酿酒酵母菌、实用念珠菌和平菇单细胞蛋白生产中的生物活化研究
British biotechnology journal Pub Date : 2016-01-10 DOI: 10.9734/bbj/2016/22509
I. Giavasis, K. Petrotos
{"title":"Biovalorization of Olive Mill Waste Water for the Production of Single Cell Protein from Saccharomyces cerevisiae, Candida utilis and Pleurotus ostreatus","authors":"I. Giavasis, K. Petrotos","doi":"10.9734/bbj/2016/22509","DOIUrl":"https://doi.org/10.9734/bbj/2016/22509","url":null,"abstract":"","PeriodicalId":90120,"journal":{"name":"British biotechnology journal","volume":"11 1","pages":"1-16"},"PeriodicalIF":0.0,"publicationDate":"2016-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71164173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Immediate Versus Delayed Dental Implants 即刻与延迟种植牙
British biotechnology journal Pub Date : 2016-01-10 DOI: 10.9734/bbj/2016/23453
W. Fareed
{"title":"Immediate Versus Delayed Dental Implants","authors":"W. Fareed","doi":"10.9734/bbj/2016/23453","DOIUrl":"https://doi.org/10.9734/bbj/2016/23453","url":null,"abstract":"","PeriodicalId":90120,"journal":{"name":"British biotechnology journal","volume":"11 1","pages":"1-7"},"PeriodicalIF":0.0,"publicationDate":"2016-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71164371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Properties of Aspergillus flavus Cellulase Produced from Solid State Fermentation of Brewers’ Spent Grain (BSG) as Substrate 以啤酒糟为底物固体发酵产黄曲霉纤维素酶的特性研究
British biotechnology journal Pub Date : 2016-01-10 DOI: 10.9734/BBJ/2016/24882
F. Orji, E. N. Dike, A. Lawal, A. Sadiq, F. Fashola, Y. Suberu, A. Famotemi, B. Ita, A. Ugbana, A. Adefiranye, E. Itoandon, G. Elemo
{"title":"Properties of Aspergillus flavus Cellulase Produced from Solid State Fermentation of Brewers’ Spent Grain (BSG) as Substrate","authors":"F. Orji, E. N. Dike, A. Lawal, A. Sadiq, F. Fashola, Y. Suberu, A. Famotemi, B. Ita, A. Ugbana, A. Adefiranye, E. Itoandon, G. Elemo","doi":"10.9734/BBJ/2016/24882","DOIUrl":"https://doi.org/10.9734/BBJ/2016/24882","url":null,"abstract":"","PeriodicalId":90120,"journal":{"name":"British biotechnology journal","volume":"13 1","pages":"1-9"},"PeriodicalIF":0.0,"publicationDate":"2016-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71164542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Molecular Biology of Microbial Pectate Lyase: A Review 微生物果胶裂解酶的分子生物学研究进展
British biotechnology journal Pub Date : 2016-01-10 DOI: 10.9734/BBJ/2016/24893
A. Dubey, S. Yadav, Manish Kumar, Gautam Anand, Dinesh Yadav
{"title":"Molecular Biology of Microbial Pectate Lyase: A Review","authors":"A. Dubey, S. Yadav, Manish Kumar, Gautam Anand, Dinesh Yadav","doi":"10.9734/BBJ/2016/24893","DOIUrl":"https://doi.org/10.9734/BBJ/2016/24893","url":null,"abstract":"Pectate lyase represents an important member of pectinase group of enzymes responsible for the pathogenesis and softening of plant tissues. It also has role in fruit juice clarification and in retting of natural fibers. The biochemical characterization of pectate lyases from diverse microbial sources and plants along with an insight to the protein structure has been dealt earlier but there is a lack of exclusive review on the molecular biology of pectate lyases. This review tries to fill the gap by highlighting the various aspects of molecular biology of microbial pectate lyases especially the cloning and expression of pectate lyase genes from diverse sources attempted so far. The topics covered in this review are a brief description about enzymes associated with degradation of pectin, its classification, applications, updated information about the biochemical characterization of microbial pectate lyases and cloning and expression of microbial pectate lyase genes.","PeriodicalId":90120,"journal":{"name":"British biotechnology journal","volume":"13 1","pages":"1-26"},"PeriodicalIF":0.0,"publicationDate":"2016-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71164575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 16
Apoptotic Gene Expression in Sheep Hepatocytes during Fasciola hepatica Infection (Fascioliasis) 绵羊肝片吸虫病感染时肝细胞凋亡基因的表达
British biotechnology journal Pub Date : 2016-01-10 DOI: 10.9734/bbj/2016/24186
W. Al-Megrin, W. Al-Qahtani
{"title":"Apoptotic Gene Expression in Sheep Hepatocytes during Fasciola hepatica Infection (Fascioliasis)","authors":"W. Al-Megrin, W. Al-Qahtani","doi":"10.9734/bbj/2016/24186","DOIUrl":"https://doi.org/10.9734/bbj/2016/24186","url":null,"abstract":"","PeriodicalId":90120,"journal":{"name":"British biotechnology journal","volume":"12 1","pages":"1-11"},"PeriodicalIF":0.0,"publicationDate":"2016-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71164789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Isolation of a Major Antimicrobial Compound from Stem Bark of Glossonema boveanum (Decne) 从牛舌茎皮中分离出一种主要抗菌化合物
British biotechnology journal Pub Date : 2016-01-10 DOI: 10.9734/bbj/2016/24436
M. Sallau, A. Uttu, H. Ibrahim, A. Idris, H. Dama
{"title":"Isolation of a Major Antimicrobial Compound from Stem Bark of Glossonema boveanum (Decne)","authors":"M. Sallau, A. Uttu, H. Ibrahim, A. Idris, H. Dama","doi":"10.9734/bbj/2016/24436","DOIUrl":"https://doi.org/10.9734/bbj/2016/24436","url":null,"abstract":"","PeriodicalId":90120,"journal":{"name":"British biotechnology journal","volume":"16 1","pages":"1-10"},"PeriodicalIF":0.0,"publicationDate":"2016-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71164828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 6
首页 上一页 下一页 尾页
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整，为了账户安全，请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
请完成安全验证×
相关产品
×
本文献相关产品
联系我们：info@booksci.cn Book学术提供免费学术资源搜索服务，方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号：481959085
Book学术官方微信