外源ABA对番茄果皮番茄红素及内源激素含量的影响

Yang Yu, Q. Weng, Bao-li Zhou
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引用次数: 2

摘要

目的:研究ABA(脱落酸)对番茄果实成熟过程中番茄红素和内源外周果皮含量的调控作用,为提高番茄果实品质提供有效措施。研究设计:当第二簇番茄果实中的番茄素达到绿色成熟期时,对果实进行标记,并在果实表面喷洒ABA (100 mg·L),以喷水为对照,避免污染叶片。处理0、4、8、12、16、20d后,取10个果实,解剖外周果皮,测定番茄红素、GA3(赤霉素酸)、ABA、ZT(玉米素)和ETH(乙烯)含量。学习地点及时间:园艺学院,2014年2月至2015年3月。方法:采用高效液相色谱法测定番茄红素、GA3、ABA和ZT的含量。采用气相色谱法测定乙醚的含量。结果:外源ABA对番茄外周果皮番茄红素含量的影响植物学报,16(1):1-5,2016;文章no.BBJ。28767 2在果实成熟期先抑制后促进。外源ABA降低了内源GA3含量,但内源ABA含量先抑制后促进,最后抑制,并促进内源ZT含量的降低。峰值出现较早,但对ETH释放量影响不大。结论:外源外源ABA可提高番茄果实中番茄红素的含量。ABA通过降低内源GA3、ZT和ABA含量,并通过内源ETH释放峰的出现促进番茄红素的积累。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effects of Exogenous ABA on Contents of Lycopene and Endogenous Hormone in Tomato Pericarp
Aims: This research studied the regulatory effect of ABA (Abscisic acid) on the contents of lycopene and endogenous hperipheral pericarp of tomato fruit during ripening, and provided an effective measure for improving the quality of tomato fruits. Study Design: When the second cluster of toormone in mato fruits reached green mature stage, fruits were labeled and sprayed ABA (100 mg·L) over fruit surface, with spaying water as control, avoiding polluting the leaves. After treatment for 0, 4, 8, 12, 16, 20d, 10 fruits were sampled and dissected, peripheral pericarp for measuring the contents of lycopene, GA3 (Gibberellic acid), ABA, ZT (Zeatin) and ETH (Ethylene). Place and Duration of Study: Horticultural College, between February 2014 and March 2015. Methodology: The contents of lycopene, GA3, ABA and ZT were determined using HPLC (High Performance Liquid Chromatography). The content of ETH was determined using GC (Gas Chromatography). Results: The effect of exogenous ABA on the lycopene content in peripheral pericarp of tomato Short Research Article Yu et al.; BBJ, 16(1): 1-5, 2016; Article no.BBJ.28767 2 fruits was firstly inhibited then promoted during fruit mature period. Exogenous ABA decreased the content of endogenous GA3, but the endogenous ABA content was inhibited firstly, and then promoted, inhibited finally, and promoted the content decrease of endogenous ZT. The peak value was appeared early, but a little effect on the amount of ETH release. Conclusion: This work proved the feasibility of elevating of lycopene levels in tomato fruit by the application of exogenous ABA. ABA promoted the accumulation of lycopene by the decreasing of endogenous GA3, ZT and ABA contents, and by releasing peak appear of endogenous ETH.
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