S. Lowor, Daniel Yabani, Kumi Winifred, C. K. Agyente-Badu
{"title":"Production of Wine and Vinegar from Cashew (Anacardium occidentale) “Apple”","authors":"S. Lowor, Daniel Yabani, Kumi Winifred, C. K. Agyente-Badu","doi":"10.9734/bbj/2016/23366","DOIUrl":"https://doi.org/10.9734/bbj/2016/23366","url":null,"abstract":"","PeriodicalId":90120,"journal":{"name":"British biotechnology journal","volume":"12 1","pages":"1-11"},"PeriodicalIF":0.0,"publicationDate":"2016-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71164477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Contribution of GFP expressing Dermal Papillae cells to the formation of chimeric embryos and their survival in uterine environment.","authors":"A. Madich, G. Richardson, C. Jahoda","doi":"10.9734/BBJ/2016/23393","DOIUrl":"https://doi.org/10.9734/BBJ/2016/23393","url":null,"abstract":"Aims: This is an innovative cell-based research project focused on the hair follicle dermal papillae (DP) as a source of key-element cells for regeneration of hair growth. DP is major dermal compartment which has a role in hair formation at embryogenesis and is able to differentiate down an endothelial lineage for in vitro functional assays. In the present study, we have tested the potential of DP cells from the lower end bulb region of hair follicles for multilineage differentiation in vivo. We have postulated that DP cells’ epigenetics can be changed under the influence of embryonic microenvironment when they are injected into early embryos which would demonstrate plasticity, regenerative and inductive properties of hair follicle dermal cells. Original Research Article Madich et al.; BBJ, 12(2): 1-12, 2016; Article no.BBJ.23393 2 Study Design: Pilot research. Place and Duration of Study: School of Biological and Biomedical Sciences, University of Durham, the UK, between February 2007 and December 2009. Methodology: To identify the capacity of functional contribution to organogenesis we microinjected various numbers of Green Fluorescence Protein (GFP)-expressing Dermal Papillae cells (GFP-DP cells) into mice blastocysts and transferred embryos to the foster mothers for the generation of chimeric fetuses. Results: GFP-expressing cells were detected in 3% of the epiblast egg cylinders formed from microinjected blastocysts cultured in absence or presence of mouse embryonic fibroblasts (MEFs). Surgical transfer of microinjected blastocysts resulted in 4.5% of fetuses developing fully. GFPDP cell lineages were detected in tissue samples under fluorescence and confirmed immunohistochemically. Our finding displayed subcultural localization of GFP as a proof of chimaeric tissue from unborn pups carrying DP cell lineages which had multiplied in bone marrow, parenchyma and connective tissue, brain and hair bulbs. Conclusion: The results confirm the capability of a small population of DP cells to convert into embryonic stem-like cells, multiply and contribute to organs and tissue. This makes them a reasonable source for regenerative studies and replacement therapy.","PeriodicalId":90120,"journal":{"name":"British biotechnology journal","volume":"12 1","pages":"1-12"},"PeriodicalIF":0.0,"publicationDate":"2016-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71164501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Ismail, N. Sidkey, R. Arafa, R. Fathy, A. El-Batal
{"title":"Evaluation of in vitro Antifungal Activity of Silver and Selenium Nanoparticles against Alternaria solani Caused Early Blight Disease on Potato","authors":"A. Ismail, N. Sidkey, R. Arafa, R. Fathy, A. El-Batal","doi":"10.9734/bbj/2016/24155","DOIUrl":"https://doi.org/10.9734/bbj/2016/24155","url":null,"abstract":"","PeriodicalId":90120,"journal":{"name":"British biotechnology journal","volume":"12 1","pages":"1-11"},"PeriodicalIF":0.0,"publicationDate":"2016-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71164722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Akami, C. Niu, H. Chakira, Zhenzhong Chen, T. Vandi, E. Nukenine
{"title":"Persistence and Comparative Pesticidal Potentials of Some Constituents of Lippia adoensis (Hochst. ex Walp.) (Lamiales: Verbenaceae) Essential Oil against Three Life Stages of Callosobruchus maculatus (Fab.) (Coleoptera: Bruchidae)","authors":"M. Akami, C. Niu, H. Chakira, Zhenzhong Chen, T. Vandi, E. Nukenine","doi":"10.9734/BBJ/2016/26087","DOIUrl":"https://doi.org/10.9734/BBJ/2016/26087","url":null,"abstract":"Over the past decades, the development of an alternative and eco-friendly pest control strategies has become a public concern for the sake of mankind. Plant essential oils are complex mixtures of volatile organic compounds, which play indispensable roles in the environment, for the plant itself, as well as for humans. Aims: The objectives of this study were (i) to identify and report the volatile organic compounds of Original Research Article Akami et al.; BBJ, 13(4): 1-16, 2016; Article no.BBJ.26087 2 Lippia adoensis (Hochst. Ex Walp.) (Lamiales: verbenaceae) essential oil (EO, herein after) and to compare the bioactivity of its four major compounds with the crude EO at relatively low dosages on some fitness parameters of Callosobruchus maculatus (F.) (Coleptera: Bruchidae), (ii) to test the persistency of each compounds over time, and (iii) to evaluate the type of interaction of the major constituents of the EO (whether antagonistic, synergistic or additive) in order to project the biological interaction of the whole compounds. Study Design: Young leaves of L. adoensis were randomly collected in Mbe locality (NgaoundereCameroon) for EO extraction which was then analysed by the means of Gas Chromatography (GCFID) coupled with Mass Spectrometry (GC-MS). Bioassays consisted of three shelves treatments for adult mortality, eggs laying, larval development and progeny production. A complete randomized design (CRD) with five replications was set for each treatment. Places and Duration of Study: Laboratory of Biology of the Faculty of Science, University of Ngaoundere (Cameroon) and Pest Control laboratory of Professor Niu Changying HZAU, Wuhan (China) from February 2014 to January 2016. Methodology: Essential oil was extracted with a Clevencher-type apparatus, hydrodistilled and airdried. Gas Chromatography-Flame Ionization Detector (GC-FID) and Gas Chromatography coupled with Mass Spectrometry (GC-MS) were carried out to analyze the constituents. The toxicity of crude EO and its four major constituents was evaluated at 0.5, 5, 10, 20 and 40 μL/g. Ten glass jars (volume 800mL) containing 50 g of cowpea seeds were prepared. After treatment, ten couples of C. maculatus aged 1-7days were randomly selected and separately introduced in each glass jar and kept at 22.72±1.06°C, 83.73±1.28% RH. Control ja rs were treated only with pure acetone. Each treatment was replicated five times. The exposure lasted for six days post treatment. Data on adult’s mortality, eggs laying, larvae and progeny production were assessed and monitored. Results: The GC–MS analysis allowed the identification of 43 volatile components representing 93.54% of total oil. The major components were Thymol (22.01%), Thymol-acetate (15.21%), paracymene (13.85%) and Triacetin (9.131%). The crude EO suppressed adults, completely inhibited eggs laying and adult emergence at 5 and 10 L/g after 24 h, respectively. Complete adults suppression was observed with thymol and the mixture at 20 L/g. Pa","PeriodicalId":90120,"journal":{"name":"British biotechnology journal","volume":"13 1","pages":"1-16"},"PeriodicalIF":0.0,"publicationDate":"2016-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71165058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Shoaib Mahmud Shaon, Pomeuz Zaman, S. Mahmud, Parsha Shanzana, Rabiul Rajib, Tawhidur Rahman, M. Islam, M. Sardar, M. Islam, M. Kabir, Selina Lira
{"title":"Detection of many Health Hazardous Chemicals Used in Tomato Marketing in Bangladesh","authors":"Shoaib Mahmud Shaon, Pomeuz Zaman, S. Mahmud, Parsha Shanzana, Rabiul Rajib, Tawhidur Rahman, M. Islam, M. Sardar, M. Islam, M. Kabir, Selina Lira","doi":"10.9734/BBJ/2016/26489","DOIUrl":"https://doi.org/10.9734/BBJ/2016/26489","url":null,"abstract":"","PeriodicalId":90120,"journal":{"name":"British biotechnology journal","volume":"15 1","pages":"1-12"},"PeriodicalIF":0.0,"publicationDate":"2016-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71165142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effects of the Combined Treatment of Ethanol Extract of Moringa oleifera and Pleurotus ostreatus on Blood Glucose, Amylase and Lipid Profile of Alloxan-induced Diabetic Wistar Albino Rats","authors":"T. Nnadiukwu, C. Monago, L. Chuku","doi":"10.9734/BBJ/2016/27365","DOIUrl":"https://doi.org/10.9734/BBJ/2016/27365","url":null,"abstract":"","PeriodicalId":90120,"journal":{"name":"British biotechnology journal","volume":"61 1","pages":"1-9"},"PeriodicalIF":0.0,"publicationDate":"2016-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71165449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Charnelle L. Djeukam, G. Theriault, P. Michael, K. Nkongolo
{"title":"Analysis of Gene Expression Associated with Copper Toxicity in White Birch (Betula papyrifera) Populations from a Mining Region","authors":"Charnelle L. Djeukam, G. Theriault, P. Michael, K. Nkongolo","doi":"10.9734/BBJ/2016/28425","DOIUrl":"https://doi.org/10.9734/BBJ/2016/28425","url":null,"abstract":"","PeriodicalId":90120,"journal":{"name":"British biotechnology journal","volume":"15 1","pages":"1-10"},"PeriodicalIF":0.0,"publicationDate":"2016-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71165880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
D. Ouattara, Honoré G. Ouattara, J. Adom, B. G. Goualié, G. Koua, G. Doué, S. Niamké
{"title":"Screening of Lactic Acid Bacteria Capable to Breakdown Citric Acid during Ivorian Cocoa Fermentation and Response of Bacterial Strains to Fermentative Conditions.","authors":"D. Ouattara, Honoré G. Ouattara, J. Adom, B. G. Goualié, G. Koua, G. Doué, S. Niamké","doi":"10.9734/BBJ/2016/19279","DOIUrl":"https://doi.org/10.9734/BBJ/2016/19279","url":null,"abstract":"","PeriodicalId":90120,"journal":{"name":"British biotechnology journal","volume":"10 1","pages":"1-10"},"PeriodicalIF":0.0,"publicationDate":"2016-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71163536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Molecular Characterization and Potential of Fungal Species Associated with Cassava Waste","authors":"A. Elijah, N. Asamudo","doi":"10.9734/bbj/2016/16559","DOIUrl":"https://doi.org/10.9734/bbj/2016/16559","url":null,"abstract":"","PeriodicalId":90120,"journal":{"name":"British biotechnology journal","volume":"10 1","pages":"1-15"},"PeriodicalIF":0.0,"publicationDate":"2016-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71163920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}