Biochemistry and analytical biochemistry : current research最新文献_第2页

Cellular Toxicity Mechanisms of TiCN-Cp-Ti Screw on Tooth Gum Cells in Rat TiCN-Cp-Ti螺钉对大鼠牙牙龈细胞的细胞毒性机制

Biochemistry and analytical biochemistry : current research Pub Date : 2021-01-01 DOI: 10.35248/2161-1009.21.10.404

P. Naserzadeh, A. Razmi, R. Yeşildal, B. Ashtari

{"title":"Cellular Toxicity Mechanisms of TiCN-Cp-Ti Screw on Tooth Gum Cells in Rat","authors":"P. Naserzadeh, A. Razmi, R. Yeşildal, B. Ashtari","doi":"10.35248/2161-1009.21.10.404","DOIUrl":"https://doi.org/10.35248/2161-1009.21.10.404","url":null,"abstract":"Cellular toxicity mechanism of TiCN film deposited on Cp-Ti substrate by Cathodic Arc Physical Vapor Deposition (CAPVD) method has yet to be clarified. The present study is aimed at finding out the possible effects of synthesized TiCN on the isolated tooth gum cells and realizing its cytotoxicity mechanism under experimental conditions compared to that of Au screws (standard in medicine). Results of the XRD analysis showed that TiCN film was formed in coating. The SEM image of TiCN film displayed a rough and irregular morphology and fine-grained surface. In addition, some other features such as cell viability, the level of Reactive Oxygen Species (ROS), lipid peroxidation (MDA), Glutathione Count (GSH and GSSG), Adenosine Triphosphate (ATP) were examined. On the other hand, activity of succinate dehydrogenase (complex II), NADH dehydrogenase (complex I), Coenzyme Q-cytochrome c reductase/Cytochrome b (complex III) and cytochrome c oxidase (complex IV) were also determined. Finally, it was also determined that level of Alkaline Phosphatase (ALP), Aspartate Amino Transferase (AST), Alanine Aminotransferase (ALP), Urea, Creatinine Clearance (CR) in rat (tooth gum cells) could also change. The results of the study revealed that TiCN coating did not incur an extremely change on cellular toxicity biomarker compared to Au standard screw in animal study. Our study provides the first evidence that TiCN coating is a biocompatible material in the cell. This paper suggests that the use of the TiCN in human dental applications need further examinations in a wide range of cellular-death signaling.","PeriodicalId":89896,"journal":{"name":"Biochemistry and analytical biochemistry : current research","volume":"10 1","pages":"1-8"},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69981465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

By Spike Investigations, DNA was Removed from Several Chromatographic Supports Used for EPO Purification 通过刺突调查，从几种用于EPO纯化的色谱支架中去除DNA

Biochemistry and analytical biochemistry : current research Pub Date : 2021-01-01 DOI: 10.35248/2161-1009.21.10.E174

Touharoki Saki

引用次数: 0

Technologies for Invadosome Analysis: An Editorial 侵入体分析技术:社论

Biochemistry and analytical biochemistry : current research Pub Date : 2021-01-01 DOI: 10.35248/2161-1009.21.10.E177

Touharoki Saki

引用次数: 0

Targeted Liposomes: Recent Applications in Human Welfare 靶向脂质体:在人类福利中的最新应用

Biochemistry and analytical biochemistry : current research Pub Date : 2021-01-01 DOI: 10.35248/2161-1009.21.10.402

Ashok Vanagamudi, Padmini Jeyarajan

引用次数: 0

Associations between Trabecular Bone Score and Biochemistry 骨小梁评分与生物化学的关系

Biochemistry and analytical biochemistry : current research Pub Date : 2021-01-01 DOI: 10.35248/2161-1009.21.10.E172

Mika Shirbasu

引用次数: 1

General Introduction on Biochemistry and its Applications 生物化学概论及其应用

Biochemistry and analytical biochemistry : current research Pub Date : 2021-01-01 DOI: 10.35248/2161-1009.21.10.E176

Susanne J Kuhel

引用次数: 0

Monocyte/Macrophage - like cell differentiation induced by TPA in HL60 cells leads to loss of Histone H4 Lysine 16 Acetylation TPA诱导HL60细胞单核/巨噬细胞样细胞分化导致组蛋白H4赖氨酸16乙酰化缺失

Biochemistry and analytical biochemistry : current research Pub Date : 2020-01-01 DOI: 10.35248/2161-1009.19.8.388

R. K. Vempati

{"title":"Monocyte/Macrophage - like cell differentiation induced by TPA in HL60 cells leads to loss of Histone H4 Lysine 16 Acetylation","authors":"R. K. Vempati","doi":"10.35248/2161-1009.19.8.388","DOIUrl":"https://doi.org/10.35248/2161-1009.19.8.388","url":null,"abstract":"12-O-Tetradecanoylphorbol-13-acetate (TPA) is a phorbol ester and induces monocyte/macrophage like cell differentiation in HL60 cells. The levels and patterns of gene expression differ greatly between differentiated and undifferentiated HL60 cells. Epigenetic histone modifications play a crucial role in transcriptional activation and alterations in the modification levels greatly affect the gene expression pattern. Acetylation of Histone H4 Lysine 16 (H4K16ac) is one such modification and has a significant role in transcriptional activation. Changes in its acetylation level either due to a physiological or pathological effect will have a dramatic effect on cellular gene expression. Here, a study was done to see the effect of TPA induced differentiation on H4K16ac levels in HL60 cells. Results obtained from flow cytometric analysis showed expression of macrophage cell surface marker CD11b on TPA differentiated HL60 cells and the western blots revealed a drastic downregulation of H4K16ac in differentiated cells. Immunoblotting and co-immunoprecipitation assay revealed DNA damage dependent enhancement of H4K16 acetylation and its co-localization with γH2AX in undifferentiated cells. Whereas, TPA differentiated cells (CD11B+ve) didn’t show any such enhancement in H4K16acetylation levels in the presence of DNA damage. The present study shows that TPA induced differentiation of HL60 cells into macrophages leads to the downregulation of H4K16 acetylation.","PeriodicalId":89896,"journal":{"name":"Biochemistry and analytical biochemistry : current research","volume":"9 1","pages":"1-4"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69980767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Colorimetric Analytical Probe for Determination of Formaldehyde and its Validation using a Single Reagent 比色分析探针测定甲醛及其单试剂验证

Biochemistry and analytical biochemistry : current research Pub Date : 2020-01-01 DOI: 10.35248/2161-1009.20.9.389

A. Shivakumar

引用次数: 0

Analytical Comparison between Spectrophotometer and Portable Glucometer for Measurement of Blood Glucose in Horse 分光光度计与便携式血糖仪测定马血糖的分析比较

Biochemistry and analytical biochemistry : current research Pub Date : 2019-08-21 DOI: 10.21203/rs.2.13351/v1

Yoseph Cherinet Megerssa, Nanoshe Taye Jima

引用次数: 0

Plasma Enzymes and Electrolytes in Heterobranchus bidorsalis Treated with Cypermethrin 氯氰菊酯对多色异枝螟血浆酶和电解质的影响

Biochemistry and analytical biochemistry : current research Pub Date : 2019-01-01 DOI: 10.35248/2161-1009.19.8.380

Gabriel Uu, E. Os, E. Egobueze

{"title":"Plasma Enzymes and Electrolytes in Heterobranchus bidorsalis Treated with Cypermethrin","authors":"Gabriel Uu, E. Os, E. Egobueze","doi":"10.35248/2161-1009.19.8.380","DOIUrl":"https://doi.org/10.35248/2161-1009.19.8.380","url":null,"abstract":"Heterobranchus bidorsalis (mean total length 31.50 ± 2.32 cm SD; mean weight 241.25 ± 30.39 g SD) were exposed to graded levels solutions of cypermethrin concentrations (0.0005, 0.0075, 0.010, 0.125 and 0.0150 ppm) and a control for 23 days. Plasma collected from the fish was analyzed for enzymes; aspartate transferase (AST), alanine transferase (ALT) and alkaline phosphatase (ALP) and electrolytes (sodium (Na+), potassium (K+) and chloride (Cl-) ions. There was a non-concentration dependent increase in ALT activity above the control value of 7.00 ± 3.83IU/L with a peak at 0.005 ppm (16.50 ± 14 IU/L), which was 135.71% above the control value. AST had variable responses with a rise in activity at 0.005 and 0.010 ppm, which were 8.26 and 5.79% above the control value of 30.25 ± 5.38IU/L. A rise in ALP was only noted at 0.005 ppm (37.75 ± 30.40IU/L), being 115.71% above the control value. A concentrationdependent inhibition was recorded from 0.0075-0.0150 ppm with the greatest inhibition of 88.57% at 0.0150 ppm. Sodium ion level was raised between 0.0075-0.0150 ppm concentrations, with a peak value at 0150 ppm, which was 11.25 units (44.55%) above the control value. A drop of 5.85 units below the control value occurred at 0.005 ppm. All the toxicant solutions caused elevation in potassium ion concentration above the value observed in the control (4.90 ± 1.83 mmol/L). Chloride ion levels was raised at 0.005 and 0.0075 ppm, which were 7.93 and 5.05% respectively above the control value (104.00 ± 10.71 mmol/L). Beyond this, there was a concentration-dependent decline in ionic level with the lowest level of 71.50 ± 13.20 mmol/L at the highest concentration. The study suggests that sublethal cypermethrin levels could cause hormone and electrolyte imbalance in the exposed fish, hence caution should be applied in its application especially near water.","PeriodicalId":89896,"journal":{"name":"Biochemistry and analytical biochemistry : current research","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69979982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5