Journal of stem cell research & therapy最新文献

{"title":"Adult Stem Cell Subsets from Adult Human Dermis","authors":"Agustin Vega-Crespo, B. Truong, Benjamen E Schoenberg, Alexandra K Ciminera, B. Larson, D. Anderson, J. Byrne","doi":"10.4172/2157-7633.1000411","DOIUrl":"https://doi.org/10.4172/2157-7633.1000411","url":null,"abstract":"Adult stem cells possess the ability to differentiate and mature into defined cell types; however, tissue-specificity and donor and culture inconsistencies have presented a challenge in identifying these cells. Adult adherent dermal cell-products have been efficiently utilized for isogenic cosmetic therapies. The purpose of this study is to identify, isolate, and characterize progenitor subsets from adult adherent dermal cells capable of ex vivo differentiation. LAVIV® adult dermal cells were independently immunoselected for CD146, CD271, and CD73/CD90/CD105 to investigate the mesenchymal differentiation capacity and possible enrichment in the purified fractions. After differentiation, the osteogenic, chondrogenic, and adipogenic potential and cell-specific gene expression were evaluated and compared for each phenotype. Adult dermal cells possess the ability to differentiate into the three cell lineages, osteocyte, chondrocyte, and adipocyte that co-express the adult stem cell immunophenotypic markers CD146 and CD271 with independent enrichment of the multipotent capacity for both fractions. We conclude that subpopulations in human dermal primary cultures possess the potential to differentiate into other cell types providing a novel source of multipotent cells for regenerative medicine.","PeriodicalId":89694,"journal":{"name":"Journal of stem cell research & therapy","volume":"8 1","pages":"1-10"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4172/2157-7633.1000411","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70402668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Stress Induced by Anesthesia and Surgery on Peripheral Blood Mobilization of Stem Cells in Horses","authors":"J. R. Gutti, B. J. William, R. George, T. A. Kannan, G. Rao, V. Leela","doi":"10.4172/2157-7633.1000440","DOIUrl":"https://doi.org/10.4172/2157-7633.1000440","url":null,"abstract":"","PeriodicalId":89694,"journal":{"name":"Journal of stem cell research & therapy","volume":"8 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70403737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Does Exosomes Derived Bone Marrow Mesenchymal Stem Cells Restore Ovarian Function by Promoting Stem Cell Survival on Experimentally Induced Polycystic Ovary in Adult Female Albino Rats? (Histological and Immunohistochemical Study)","authors":"E. Faruk, R. El-desoky, A. Al-Shazly, Nm Taha","doi":"10.4172/2157-7633.1000442","DOIUrl":"https://doi.org/10.4172/2157-7633.1000442","url":null,"abstract":"Poly Cystic Ovarian Syndrome (PCOS) is increasingly reported nowadays. Recently exosomes released by Mesenchymal Stem Cells (MSCs-EX) has been a novel source with a great potential donor cell in regenerative medicine because of their low immunogenicity and easy accessibility. Foeniculum vulgare (FVE) is a naturally occurring estrogen compound, which is commonly used today due to the impact of the female hormones. In this study, we evaluated the therapeutic effect of exosome released by MSCs in experimentally induced Poly Cystic Ovary (PCO). The polycystic disease was artificially induced by injecting rats daily with testosterone propionate (dissolved in propylene glycol) 1 mg/100 g body weight for 35 days. Exosomes were prepared for 3rd passage of MSCs-EX and were injected into the induced PCO rats. Another group of PCOS rats received FVE (alcoholic extract) 150 mg/kg body weight/day intra gastric for five days after induction of PCOS. The ovaries were taken for histological examination and immune-histochemical detection of Octamer-Binding Transcription Factor (OCT4), and the hormonal assay was evaluated. Both groups infused with Bone Marrow Mesenchymal Stem Cells (BM-MSCs) derived exosome and FVE have mild to moderate improvement in the histological ovarian structure by the presence of normal follicles in different stages with normal hormonal profile and highly expressive of OCT4. BM-MSCs derived exosome and FVE have moderate modulates the immunohistological structure of the PCOS ovaries, which may be a factor in the maintenance of steroid-induced PCOS.","PeriodicalId":89694,"journal":{"name":"Journal of stem cell research & therapy","volume":"8 1","pages":"1-7"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4172/2157-7633.1000442","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70403474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quantitative and Qualitative Analysis of Human Stromal Vascular Fraction from Different Methods of Liposuction Short Title: Stromal Fraction from Liposuction Types","authors":"Luiz Fern, O. Frascino, I. Filho","doi":"10.4172/2157-7633.1000439","DOIUrl":"https://doi.org/10.4172/2157-7633.1000439","url":null,"abstract":"Background: Although the widespread use for harvesting fat, liposuction is not represented by a single procedure and, in the absence of evidence based guidelines regarding cell based therapies, different protocols may be employed to extract the Stromal Vascular Fraction (SVF) and its cellular subpopulations. Usually the tissue-harvesting procedures has been underestimated as a factor to impact the outcomes.Methods: 4 methods of liposuction were employed in triplicate in 16 patient candidates for liposuction: Manual liposuction with 10.0 mL Luer-Lok syringe and 2.0 mm blunt tip cannula Manual liposuction with 60.0 mL syringe and 2.5 mm cannula Power-Assisted Liposuction (PAL) (Vibrofit®- Faga Medical) set at 3.000 cycles and 3.0 mm cannula and Suction-Assisted Liposuction (SAL) ( Nevoni® 3003 model) and 4.0 mm cannula They were divided in 3 groups, according to the aspirated volume:- group I: 20.0 mL, group II: 60.0 mL and group III: 120.0 mL, obtaining 48 samples for analysis. Cellular quantification, viability and mesenchymal characterization was performed in the extracted SVF in all samples and the results were compared by Pearson statistical test and logistic probability, adopting α significant level above 0.05 (α>0.05).Results: The worst cell yielding was obtained with SAL and 4 mm blunt tip cannulas in all volumes. The manual method with 10.0 mL seringe/2.0 mm cannula and PAL/3.0 mm cannula showed better cellular SVF extraction in all groups, with no differences statistically significant between them. The cell frequencies of these best scores showed an exponential increase with increasing volumes from 2.900.000 cells/20 mL group to 18.500.000/60 mL group (6.4xx) and to 380.000.000/120 mL group (20.5xx).Conclusion: The mechanical stress applied over the subcutaneous tissue may impact the cell yielding of the extracted SVF. Syringes with small cannulas (2.0 mm), and/or in-vivo emulsification of the adipose tissue through PAL seem to have a positive effect, optimizing future liposuction protocols.","PeriodicalId":89694,"journal":{"name":"Journal of stem cell research & therapy","volume":"21 1","pages":"1-7"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70403679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cancer stem cells (CSC), genetic drivers and therapeutic targeting via CCR5","authors":"pRichard G Pestellp","doi":"10.4172/2157-7633-C5-043","DOIUrl":"https://doi.org/10.4172/2157-7633-C5-043","url":null,"abstract":"","PeriodicalId":89694,"journal":{"name":"Journal of stem cell research & therapy","volume":"08 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70410491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization of Alginate-Based Encapsulation Utilization For Viability and Stability of The Mesenchymal Stem Cell","authors":"R. Rilianawati, H. Ago, Subintoro, R. Elrade, A. Kurnia","doi":"10.4172/2157-7633.1000412","DOIUrl":"https://doi.org/10.4172/2157-7633.1000412","url":null,"abstract":"In the past few decades, attention and research in the field of stem cell are progressing very rapidly. Hospitals in Indonesia have been using stem cells as an alternative to cure some illnesses like diabetes, heart disease, fractures and joints, dental implants, etc. Currently, adult stem cells can be obtained not only from the spinal cord and peripheral vessels, but also from fat tissues of the human body, where it can be isolated as adherent stem cells (mesenchymal stem cells). Consideration of fat tissue as the source of mesenchymal stem cells (MSCs) for autologous tissue engineering is because they are readily available in abundant quantities through minimal invasive procedures, as well as easily cultured and propagated. It is possible to proliferate and differentiate into the desired direction of the network. Stem cell growth requires conditions to grow such as requiring optimum growing conditions such as an environmental temperature of 37°C and a concentration of 5% CO2. Maintenance of MSCs also requires a subculture process, i.e. the process of moving MSCs from a full culture medium to new media; continuous subculture process can cause changes in MSCs. The viability of stem cells may be disrupted by micro-conditions in wounds such as hypoxia, oxidative stress, and inflammation. Therefore, the purpose of this research was to investigate whether alginate-based encapsulation can increase and maintenance stem cell growth at different temperature by using some concentration of alginate and CaCl2 as the formula. Results shown that alginat with low concentration and CaCl2 100mM is suitable for MSCs growth (as in MTT result shown) at 25°C temperature. This can be due to the MSCs encapsulated can adapt and grow within the alginate microcapsule with low concentration. In addition, the media may also easier to get into the microcapsule alginate.","PeriodicalId":89694,"journal":{"name":"Journal of stem cell research & therapy","volume":"8 1","pages":"1-14"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4172/2157-7633.1000412","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70402718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Kruppel-Like Factor 4 (KLF4) and its Regulation on Mitochondrial Homeostasis.","authors":"Brian Tung,&nbsp;Shuli Xia","doi":"10.4172/2157-7633.1000436","DOIUrl":"https://doi.org/10.4172/2157-7633.1000436","url":null,"abstract":"<p><p>Mitochondria are vital organelles that supply ATP and other energy metabolites to meet the bioenergetics demands of the cell. In environments of stress or increased energy requirement, mitochondria are highly dynamic and can undergo biogenesis, fusion/fission, or autophagy. The transcription factor family, Kruppel-Like Factor (KLF), is necessary to carry out normal cellular processes from proliferation to differentiation. Recently, its importance in metabolic homeostasis in various tissue types has gained much attention. A handful of evidence supports KLF4's involvement in regulating mitochondrial homeostasis in both healthy and cancer cells. In this review, we aim to summarize the available literature that demonstrates KLF4's ability to modulate the mitochondrial life cycle in: Cardiac tissue, in which KLF4-knockdown subsequently leads to Heart Failure (HF), andGlioblastoma (GBM), where its expression promotes extensive mitochondrial fusion and offers mild cell protection under serum-deprivation.</p>","PeriodicalId":89694,"journal":{"name":"Journal of stem cell research & therapy","volume":"8 9","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6594694/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37367050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metadichol ® and CD34 Expression in Umbilical Cord Cells","authors":"R. Pr","doi":"10.4172/2157-7633.1000409","DOIUrl":"https://doi.org/10.4172/2157-7633.1000409","url":null,"abstract":"Umbilical cord blood has found use in the clinic for more than 40 years in hematopoietic stem cell transplantation therapies to treat patients with bone marrow diseases or to reconstitute the bone of those cancer patients who had to have theirs wiped out to cure their leukemia or lymphoma. A feature is the presence of CD34 antigen in of hematopoietic stem and progenitor cells. These cells can differentiate and are self-renewing, multipotent stem cells that give rise to all blood cells of the immune system and erythrocytes), and lymphoid (T cells, B cells, and NK cells) lineages. This study describes increased CD34 gene expression in Umbilical Cord (UC) cells upon treatment with Metadichol which is an inverse agonist of AHR (Aryl Hydrocarbon Receptor). UC cells were subjected to treatment at one picogram, 100 picograms, 1 nanogram, 100 nanograms and 1 microgram per ml of Metadichol for 72 hrs. Cells treated at 1ng have shown the highest increase in expression of CD34 compared to untreated Control. The cells treated with 1 pg, 100 picogram/ml demonstrated the multiplicity of CD34 expression as indicated by peak shift compared to treatment with 1ng, 100 ng, and 1 μg","PeriodicalId":89694,"journal":{"name":"Journal of stem cell research & therapy","volume":"8 1","pages":"1-1"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70402556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Generation of sympathoadrenal progenitor cells from human pluripotent stem cells","authors":"pNashwa Am Mostafa Kawthar M Abdel Hamid Ola A Hussien, Hanan S Farghalyp","doi":"10.4172/2157-7633-C6-048","DOIUrl":"https://doi.org/10.4172/2157-7633-C6-048","url":null,"abstract":"","PeriodicalId":89694,"journal":{"name":"Journal of stem cell research & therapy","volume":"08 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70410771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Engraftment of Reprogrammed Human Bile Duct Cells Transiently Rescues Diabetes in Mice","authors":"Anannya Banga, J. Dutton, Margaret A. Mysz, Beverly J. Norris, B. Ogle, J. Tolar, R. Schumacher, C. Flory","doi":"10.4172/2157-7633.1000432","DOIUrl":"https://doi.org/10.4172/2157-7633.1000432","url":null,"abstract":"Differentiation of adult stem/progenitor cells into functional beta cells to provide a successful autologous cell therapy for Type 1 diabetes patients has not yet been achieved. Progenitor cells in the adult pancreas or liver have been considered potential sources of endocrine beta cells based on their ability to repopulate the organ following injury. Here we describe the isolation and reprogramming of a lineage-committed progenitor population of cells in the human bile duct towards a beta cell fate. These cells, which possess SOX9 as a marker, were able to manifest beta cell characteristics upon ectopic expression of pancreatic transcription factors. The beta cells derived from SOX9+ progenitor cells were also able to ameliorate high blood glucose in diabetic mice. The insulin+ islet-like clusters which developed from this progenitor cell type demonstrate the potential of this approach to generate functional, autologous beta cells.","PeriodicalId":89694,"journal":{"name":"Journal of stem cell research & therapy","volume":"8 1","pages":"1-7"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70403196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}