Advances in genomics and genetics最新文献

{"title":"Standardized karyotype and idiogram of Thai native swamp buffalo, Bubalus bubalis (Artiodactyla, Bovidae) by convention staining, G-banding, C-banding and NOR-banding techniques","authors":"P. Supanuam, A. Tanomtong, S. Jantarat, Wanpen Kakampuy, Sarawut Kaewsri, Anan Kenthao","doi":"10.14456/TJG.2010.8","DOIUrl":"https://doi.org/10.14456/TJG.2010.8","url":null,"abstract":"Standardized karyotype and idiogram of the Thai native swamp buffalo ( Bubalus bubalis ) at Khon Kaen University, Thailand, were studied. Blood samples were taken from two male and two female swamp buffaloes. After lymphocyte culture, the mitotic chromosome preparation was accomplished by hypotonic-air-drying method. Conventional staining, G-banding, C-banding and NOR-banding techniques were applied to stain the chromosome. The results showed that diploid number of Thai native swamp buffalo was 2 n =48, the fundamental numbers (NF) were 58 in both male and female. The types of autosomes were 4 large metacentric, 4 large acrocentric, 2 small submetacentric and 36 small telocentric chromosomes. The X chromosome was large telocentric chromosome and the Y chromosome was the small telocentric chromosome. From G-banding, each chromosome pair appears with distinctively differentiated. C-banding shown C-positive (dark band) on centromere of all telocentric chromosomes but others appeared as C-negative (light band). NOR-banding exhibited 6 telocentric chromosome pairs. The karyotype formula of Thai native swamp buffalo was as follows: 2 n (diploid) 48 = L m 4 + L a 4 +S sm 2 +S t 36 + sex chromosomes","PeriodicalId":89652,"journal":{"name":"Advances in genomics and genetics","volume":"661 1","pages":"83-93"},"PeriodicalIF":0.0,"publicationDate":"2012-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76832163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic diversity of Curcuma comosa Roxb. revealed by DNA and mophological markers","authors":"S. Peyachoknagul, V. Keeratinijakal","doi":"10.14456/TJG.2010.16","DOIUrl":"https://doi.org/10.14456/TJG.2010.16","url":null,"abstract":"AFLP and morphological markers were applied to study genetic diversity of Waan Chak Modlook ( Curcuma comosa Roxb.). Thirty-three samples of Curcuma comosa Roxb. were amplified with nine Eco RI– Mse I primer combinations that generated a total of 161 bands, in which 102 of them or 63.35% of total bands were polymorphic. The UPGMA clusters derived from AFLP showed that they could be classified into two major groups. Principal coordinates analysis (PCoA) was also analyzed and the result was consistent with the grouping found in the dendrogram. The values of similarity coefficient ranged from 0.49-1.00. Morphological characteristics could be also classified into two major groups with different inflorescence.","PeriodicalId":89652,"journal":{"name":"Advances in genomics and genetics","volume":"20 1","pages":"51-59"},"PeriodicalIF":0.0,"publicationDate":"2012-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84747114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Selection of rice (Oryza sativa L.) for higher yield and quality from crosses between tropical japonica and indica","authors":"Nongyao Kaewwiset, P. Sripichitt, T. Sreewongchai, S. Junbuathong","doi":"10.14456/TJG.2010.11","DOIUrl":"https://doi.org/10.14456/TJG.2010.11","url":null,"abstract":"Selection of rice (Oryza sativa L.) for higher yield and quality was conducted by hybridization between tropical japonica rice which had low tillering capacity, large panicles with high number of grains and indica rice which possessed good grain quality including long, slender and translucent for 12 crosses. The F1 plants produced were grown in the greenhouse and the F2 to F3 progenies were planted in the experimental field at Pathum Thani Rice Research Center, Pathum Thani province. Selection was done in each succeeding generation from F2 to F3 using pedigree method for plants/lines which had high yield and good agronomic characters. Preliminary yield test of the 33 selected F4 lines, 7 parental lines and 5 check varieties (PTT1, CNT1, SPR1, SPR2 and RD31) were conducted using augmented design in randomized complete block design at Pathum Thani Rice Research Center in rainy season 2009. The results showed that there were 15 from 33 tested F4 lines which gave higher yield than the highest yielding check variety SPR1. They showed the yield varying from 621 to 1,035 kg/rai while the check variety SPR1 gave the yield of 615 kg/rai. However, there were 4 lines which gave significantly higher yield than the check variety SPR1. These lines were PTT07288-20-1, PTT07288-11-4, PTT07288-20-3 and PTT07288-14-2 which gave the yield of 1035, 974, 968 and 939 kg/rai, respectively. The 6 parental lines manifested the yield ranging from 127 to 573 kg/rai which were lower than the check variety SPR1. Grain physical quality and cooking and eating quality of the 15 F4 lines were determined. It was found that there were 4 lines including PTT0728811-4, PTT07285-21-2, PTT07266-8-6 and PTT072667-1 which exhibited good physical grain quality by having long, slender and clear grains or with slightly chalkiness. However, these lines manifested variable cooking and eating quality by having medium (20 25%) to high (25-33%) amylose content. Consequently, the texture of cooked rice varied from soft and slightly sticky to hard and loose. Selection will be done further for improving cooking and eating quality in order to meet the requirement of customers. §” ”§—≠: ¢â“« tropical japonica, ¢â“« indica, °“√§—¥ æ—π∏ÿå·∫∫ ◊∫a√–«—μ‘, √Ÿa∑√ßμâπ¢â“«·∫∫„À¡à","PeriodicalId":89652,"journal":{"name":"Advances in genomics and genetics","volume":"33 1","pages":"60-70"},"PeriodicalIF":0.0,"publicationDate":"2012-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74100335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DNA fingerprinting of eucalyptus clones using microsatellite markers","authors":"Tanarat K. Jantapanon, S. Apisitwanich, S. Peyachoknagul","doi":"10.14456/TJG.2010.10","DOIUrl":"https://doi.org/10.14456/TJG.2010.10","url":null,"abstract":"Microsatellite marker was used to verify eucalyptus clones. Thirty nine primer pairs were selected. Twenty eight primer pairs could amplify DNA in which 18 of them showed polymorphic band pattern among 25 eucalyptus clones. One hundred and fifty-two distinct DNA bands were scored and calculated for the genetic similarity and cluster analysis using simple matching and unweighed pair group method using arithmetic average (UPGMA). From the phylogenetic tree, these eucalyptus clones were divided into four clusters. Two sets of primer pairs, namely EMBRA 2 and EMBRA 22, EMBRA 2 and EMBRA 63, EMBRA 22 and EMBRA 69 or EMBRA 63 and EMBRA 69 were combined and able to simultaneously amplify DNA in a duplex PCR reaction which made fingerprinting of eucalyptus clones easier and less expensive.","PeriodicalId":89652,"journal":{"name":"Advances in genomics and genetics","volume":"12 1","pages":"31-40"},"PeriodicalIF":0.0,"publicationDate":"2012-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81999368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of microsatellite markers for Jatropha curcas L.","authors":"Yatavee Rattanamanee, S. Peyachoknagul, N. Sangduen, V. Hongtrakul","doi":"10.14456/TJG.2009.12","DOIUrl":"https://doi.org/10.14456/TJG.2009.12","url":null,"abstract":"The objective of this research is to develop microsatellite DNA markers for classification and species specific identification of physic nut ( Jatropha curcas L.). DNAs from physic nut (SS20-Sbr3, USA, Nakhon Ratchasima) were digested with Mse I restriction enzyme, then ligated to adapters. The products were PCR amplified before hybridization to biotin-oligonucleotide probes of B-(GA) 15 , B-(CA) 15 , B-(ACC) 10 and B-(CCT) 10 containing magnetic beads. The DNA fragments were then PCR amplified again before ligation to plasmid vector. The recombinant plasmids were heat-shock transformed to supercompetent E. coli JM109 cells. A total of 996 white colonies were selected and 906 clones (90.96 %) were confirmed to carry DNA inserts by PCR amplification using primers specific to the plasmid. Dot blot hybridization was performed to reconfirm the microsatellite sequences in the inserts. Ninety-seven clones were selected and sequenced. A total of 51 clones (52.58 %) were found to contain microsatellite sequences. Most abundant iterated sequences were di-nucleotide repeat of (GA) n (31.37 %) and tri-nucleotide repeat of (GGA) n (23.53 %). Twenty six primer pairs were designed and four pairs could amplify the DNA, giving the expected PCR product with polymorphism among toxic and non-toxic physic nuts.","PeriodicalId":89652,"journal":{"name":"Advances in genomics and genetics","volume":"71 1","pages":"145-154"},"PeriodicalIF":0.0,"publicationDate":"2012-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83923771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Generation mean analysis in azuki bean using matrix algebra method","authors":"W. Kunkaew, Suthat Julsrigival, C. Senthong, D. Karladee","doi":"10.14456/TJG.2008.16","DOIUrl":"https://doi.org/10.14456/TJG.2008.16","url":null,"abstract":"Six basic populations including P 1 , P 2 , F 1 , F 2 , BC 1 and BC 2 developed from three crosses of azuki bean; Hondawase x Akatsukidainagon (H x A), Hondawase x Erimo (H x E) and Akatsukidainagon x Erimo (A x E) were planted for generation mean analysis study at Pangda Royal Agricultural Station in Chiang Mai during August to December, 2006. Generation mean of these three azuki bean crosses were analysed by using matrix algebra method. Results of study indicated that matrix algebra method was able to analyse generation mean and obtained all six parameters of gene action; m, [d], [h], [i], [j] and [l] for seed yield per plant. The values of matrix algebra method for analysing generation mean were convenient efficient and simply applied to use with some softwares. It is anticipated that this matrix algebra method would be an effective way to identify the gene action parameters which these parameters will provide important basic informations for yield improvement in plants.","PeriodicalId":89652,"journal":{"name":"Advances in genomics and genetics","volume":"9 7","pages":"128-145"},"PeriodicalIF":0.0,"publicationDate":"2012-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72615533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chromosomal analysis from blood: 18 year experience of Songklanagarind Hospital","authors":"Somkhae Puangpech, Chettupon Pooljun, J. Kayman, U. Jinorose, Sinitdhorn Rujirabanjerd, P. Limprasert","doi":"10.14456/TJG.2008.17","DOIUrl":"https://doi.org/10.14456/TJG.2008.17","url":null,"abstract":"Metaphase chromosome analysis from cultured blood lymphocytes were investigated in 5,866 tests at Songklanagarind Hospital from 1990 through 2007 (18 years). Elaboration of G-bands by trypsin using Giemsa (GTG) was routinely employed with other banding techniques if required. High resolution chromosome banding was performed in suspected cases of structural chromosome abnormality. Successful studies were obtained in 5,665 tests (96.4 %) and 1,680 tests (28.7 %) were found to have chromosomal abnormalities. The failure rate was 3.4 % (201 tests). Fifty duplicated tests were discarded of the 1680 abnormal results, leaving 1,630 cases with abnormal chromosomes. Of 1,630 cases, Down, Edwards, and Patau syndromes were found in 933 (57.2 %), 87 (5.3 %) and 80 (4.9 %) cases, respectively. There were 215 cases with sex chromosome abnormalities (13.2 %), including 188 cases with Turner syndrome, 18 cases with Klinefelter syndrome, 6 cases with XXX syndrome and 3 cases with XYY syndrome. Androgen insensitivity syndrome (female with 46,XY) was found in 23 cases, and structural chromosome abnormalities were found in 222 cases (13.6 %). The remainder (70 cases/4.3 %) was composed of microdeletion, marker chromosome, fragile X syndrome and rare trisomy. To the best of our knowledge, this study is the largest group of chromosome analysis results reported from Thailand. These findings can be available to provide the database for genetic counseling.","PeriodicalId":89652,"journal":{"name":"Advances in genomics and genetics","volume":"16 1","pages":"146-162"},"PeriodicalIF":0.0,"publicationDate":"2012-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85472428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterisation of endophytic actinomycetes isolated from wattle trees (Acacia auriculiformis A. Cunn. ex Benth.) in Thailand","authors":"C. Bunyoo, Kannika Duangmal, A. Nuntagij, A. Thamchaipenet","doi":"10.14456/TJG.2009.1","DOIUrl":"https://doi.org/10.14456/TJG.2009.1","url":null,"abstract":"Eleven strains of endophytic actinomycetes were isolated from healthy roots of Acacia auriculiformis A. Cunn . ex Benth. collected from Bangkok and Nakhonpathom, Thailand. No actinomycete could be isolated from leaf samples. Analysis of 16S rRNA sequencing of those strains revealed that they belong to members of genera Streptomyces , Actinoallomurus , Amycolatopsis , Kribbella and Microbispora . Within 11 endophytic actinomycetes, GMKU 944 showed strongest antibacterial activities against tested bacteria and fungi. In this work, the residing property of endophytic actinomycetes in the roots of wattle tree was verified by inoculation of GMKU 937 to germinated seeds. It was demonstrated that GMKU 937 could be re-isolated and was a true endophyte.","PeriodicalId":89652,"journal":{"name":"Advances in genomics and genetics","volume":"12 1","pages":"155-163"},"PeriodicalIF":0.0,"publicationDate":"2012-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75558389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Blast disease and rice breeding for blast disease resistance through marker-assisted selection","authors":"ศรีสวัสดิ์ ขันทอง, ชัชวาล จันทราสุริยารัตน์, สุรีพร เกตุงาม","doi":"10.14456/TJG.2010.4","DOIUrl":"https://doi.org/10.14456/TJG.2010.4","url":null,"abstract":"","PeriodicalId":89652,"journal":{"name":"Advances in genomics and genetics","volume":"53 1","pages":"106-119"},"PeriodicalIF":0.0,"publicationDate":"2012-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86709423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Induced mutation in rice (cv. RD 6) by tissue culture","authors":"Jirapan Tongsroy, S. Peyachoknagul, P. Pongtongkam","doi":"10.14456/TJG.2008.8","DOIUrl":"https://doi.org/10.14456/TJG.2008.8","url":null,"abstract":"The sterilized seeds of sticky rice cultivar RD 6 were cultured on the MS medium supplemented with 15% coconut water, 1g/l yeast extract and 0, 2, 4, 6, 8 and 10 mg/l BAP. The suitable medium for multiple shoots induction was MS medium supplemented with 15% coconut water, 1g/l yeast extract and 2 mg/l BAP giving the average of 5.47 shoots per seed. The shoots were gamma irradiated at 0 and 30 Grays. Non-irradiated and irradiated shoots were cultured on MS medium supplemented with 2 mg/l BAP and 0, 0.5, 1.0 and 1.5% NaCl. The number of non-irradiated surviving shoots on these media were 18, 15, 1 and 0 shoots, respectively, while those of irradiated shoots on the same media were 33, 29, 6 and 2 shoots, respectively. These survival plants of both groups were then transferred to grow in the pot containing sand, Yoshida’s solution and 1% NaCl. After 4 weeks of growth, 14 out of 70 irradiated plants survived but only 3 out of 34 non-irradiated ones could tolerate the high salt condition.","PeriodicalId":89652,"journal":{"name":"Advances in genomics and genetics","volume":"102 1","pages":"32-39"},"PeriodicalIF":0.0,"publicationDate":"2012-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85477520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}