DNA fingerprinting of eucalyptus clones using microsatellite markers

Microsatellite marker was used to verify eucalyptus clones. Thirty nine primer pairs were selected. Twenty eight primer pairs could amplify DNA in which 18 of them showed polymorphic band pattern among 25 eucalyptus clones. One hundred and fifty-two distinct DNA bands were scored and calculated for the genetic similarity and cluster analysis using simple matching and unweighed pair group method using arithmetic average (UPGMA). From the phylogenetic tree, these eucalyptus clones were divided into four clusters. Two sets of primer pairs, namely EMBRA 2 and EMBRA 22, EMBRA 2 and EMBRA 63, EMBRA 22 and EMBRA 69 or EMBRA 63 and EMBRA 69 were combined and able to simultaneously amplify DNA in a duplex PCR reaction which made fingerprinting of eucalyptus clones easier and less expensive.