Induced mutation in rice (cv. RD 6) by tissue culture

The sterilized seeds of sticky rice cultivar RD 6 were cultured on the MS medium supplemented with 15% coconut water, 1g/l yeast extract and 0, 2, 4, 6, 8 and 10 mg/l BAP. The suitable medium for multiple shoots induction was MS medium supplemented with 15% coconut water, 1g/l yeast extract and 2 mg/l BAP giving the average of 5.47 shoots per seed. The shoots were gamma irradiated at 0 and 30 Grays. Non-irradiated and irradiated shoots were cultured on MS medium supplemented with 2 mg/l BAP and 0, 0.5, 1.0 and 1.5% NaCl. The number of non-irradiated surviving shoots on these media were 18, 15, 1 and 0 shoots, respectively, while those of irradiated shoots on the same media were 33, 29, 6 and 2 shoots, respectively. These survival plants of both groups were then transferred to grow in the pot containing sand, Yoshida’s solution and 1% NaCl. After 4 weeks of growth, 14 out of 70 irradiated plants survived but only 3 out of 34 non-irradiated ones could tolerate the high salt condition.